(Circular) (Six-base) MAPSORT of: pFN21AB7043.seq Check: 1417 from: 1 to: 6115 pFN21AB7043 6115 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3925 278 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3458 1937 Size: 1521 4594 Acc65I G'GTAC_C Cuts at: 3436 3436 Size: 6115 AclI AA'CG_TT Cuts at: 1563 4242 4615 1563 Size: 2679 373 3063 Fragments arranged by size: 3063 2679 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3334 4178 5562 5830 1632 Size: 1702 844 1384 268 1917 Fragments arranged by size: 1917 1702 1384 844 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5918 AflIII A'CryG_T Cuts at: 1203 5014 5991 1203 Size: 3811 977 1327 Fragments arranged by size: 3811 1327 977 AgeI A'CCGG_T Cuts at: 4946 4946 Size: 6115 AhdI GACnn_n'nnGTC Cuts at: 2307 4844 2307 Size: 2537 3578 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6115 AlwNI CAG_nnn'CTG Cuts at: 2836 2854 3858 5430 2836 Size: 18 1004 1572 3521 Fragments arranged by size: 3521 1572 1004 18 ApaI G_GGCC'C Cuts at: 2704 3270 2704 Size: 566 5549 ApaLI G'TGCA_C Cuts at: 4172 5328 4172 Size: 1156 4959 ApoI r'AATT_y Cuts at: 1493 3424 3650 3802 5911 1493 Size: 1931 226 152 2109 1697 Fragments arranged by size: 2109 1931 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4669 160 Size: 872 3637 1606 Fragments arranged by size: 3637 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 AvaI C'yCGr_G Cuts at: 1870 1943 2144 2294 2342 2492 3440 1870 Size: 73 201 150 48 150 948 4545 Fragments arranged by size: 4545 948 201 150 150 73 48 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2375 2408 3109 3142 2375 Size: 33 701 33 5348 Fragments arranged by size: 5348 701 33 33 BamHI G'GATC_C Cuts at: 3445 3812 3445 Size: 367 5748 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2281 2290 3034 3058 3436 Size: 325 217 627 494 9 744 24 378 Cuts at: 3436 4891 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 744 627 494 378 325 217 24 9 BanII G_rGCy'C Cuts at: 1391 2145 2704 2946 3270 3434 1391 Size: 754 559 242 324 164 4072 Fragments arranged by size: 4072 754 559 324 242 164 BbeI G_GCGC'C Cuts at: 2285 2294 2285 Size: 9 6106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2421 2622 2801 928 Size: 971 522 201 179 4242 Fragments arranged by size: 4242 971 522 201 179 BbvCI CC'TCA_GC Cuts at: 2779 2779 Size: 6115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4306 4340 1903 Size: 34 2369 34 3678 Fragments arranged by size: 3678 2369 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4007 5217 4007 Size: 1210 4905 BclI T'GATC_A Cuts at: 1548 1548 Size: 6115 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4726 136 Size: 107 122 71 631 605 3054 1525 Fragments arranged by size: 3054 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6110 6110 Size: 6115 BlpI GC'TnA_GC Cuts at: 3532 3532 Size: 6115 Bme1580I G_kGCm'C Cuts at: 1790 2655 2704 3015 3063 3270 4176 5332 1790 Size: 865 49 311 48 207 906 1156 2573 Fragments arranged by size: 2573 1156 906 865 311 207 49 48 BmgBI CAC'GTC Cuts at: 1318 3175 1318 Size: 1857 4258 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3846 4804 442 Size: 416 53 83 399 24 2429 958 1753 Fragments arranged by size: 2429 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6115 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2794 2794 Size: 6115 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2076 3130 3197 4775 1239 Size: 837 1054 67 1578 2579 Fragments arranged by size: 2579 1578 1054 837 67 Bpu10I CC'TnA_GC Cuts at: 2779 2779 Size: 6115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2732 2915 3131 4240 5105 5403 2732 Size: 183 216 1109 865 298 3444 Fragments arranged by size: 3444 1109 865 298 216 183 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2978 4778 882 Size: 617 1479 1800 2219 Fragments arranged by size: 2219 1800 1479 617 BsaAI yAC'GTr Cuts at: 493 1204 2476 3906 493 Size: 711 1272 1430 2702 Fragments arranged by size: 2702 1430 1272 711 BsaBI GATnn'nnATC Cuts at: 6109 6109 Size: 6115 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2282 2291 2879 3922 Size: 53 83 186 936 749 9 588 1043 Cuts at: 3922 4304 4939 275 Size: 382 635 1451 Fragments arranged by size: 1451 1043 936 749 635 588 382 186 83 53 9 BsaWI w'CCGG_w Cuts at: 2683 4548 4946 5220 5367 5778 2683 Size: 1865 398 274 147 411 3020 Fragments arranged by size: 3020 1865 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5890 Fragments arranged by size: 5890 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2702 2710 1186 Size: 1516 8 4591 Fragments arranged by size: 4591 1516 8 BseYI C'CCAG_C Cuts at: 2167 2305 2316 2874 2945 5318 5814 2167 Size: 138 11 558 71 2373 496 2468 Fragments arranged by size: 2468 2373 558 496 138 71 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5542 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2696 2738 3565 4326 5354 1540 Size: 275 186 695 42 827 761 1028 2301 Fragments arranged by size: 2301 1028 827 761 695 275 186 42 BsiHKAI G_wGCw'C Cuts at: 2663 3162 4176 4261 5332 2663 Size: 499 1014 85 1071 3446 Fragments arranged by size: 3446 1071 1014 499 85 BsiWI C'GTAC_G Cuts at: 2274 2274 Size: 6115 BsmI GAATG_Cn' Cuts at: 3626 3719 3626 Size: 93 6022 BsmBI CGTCTCn'nnnn_ Cuts at: 2090 2090 Size: 6115 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2655 2663 2704 2946 3015 3063 Size: 399 355 510 8 41 242 69 48 Cuts at: 3063 3162 3270 3434 4176 4261 5332 1391 Size: 99 108 164 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 510 399 355 242 164 108 99 85 69 48 41 8 BspEI T'CCGG_A Cuts at: 2683 5778 2683 Size: 3095 3020 BspHI T'CATG_A Cuts at: 4004 5734 4004 Size: 1730 4385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3470 844 Size: 2626 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2357 2526 2692 4002 1394 Size: 429 534 169 166 1310 3507 Fragments arranged by size: 3507 1310 534 429 169 166 BsrDI GCAATG_nn' Cuts at: 65 1248 3333 4610 4784 65 Size: 1183 2085 1277 174 1396 Fragments arranged by size: 2085 1396 1277 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2737 3040 4759 4946 1700 Size: 110 927 303 1719 187 2869 Fragments arranged by size: 2869 1719 927 303 187 110 BsrGI T'GTAC_A Cuts at: 96 3404 96 Size: 3308 2807 BssHII G'CGCG_C Cuts at: 1925 2074 1925 Size: 149 5966 BssSI C'ACGA_G Cuts at: 2656 4175 5187 2656 Size: 1519 1012 3584 Fragments arranged by size: 3584 1519 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2857 794 Size: 2063 4052 BstBI TT'CG_AA Cuts at: 4919 4919 Size: 6115 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6115 BstYI r'GATC_y Cuts at: 1972 2961 3445 3812 4197 4214 5666 5752 5764 Size: 989 484 367 385 17 1452 86 12 Cuts at: 5764 6110 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 989 484 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2085 2085 Size: 6115 BtgI C'CryG_G Cuts at: 513 1065 2004 2225 2379 2423 2580 513 Size: 552 939 221 154 44 157 4048 Fragments arranged by size: 4048 939 552 221 157 154 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2151 487 Size: 683 56 925 4451 Fragments arranged by size: 4451 925 683 56 BtsI GCAGTG_nn' Cuts at: 3002 3194 3631 4425 4445 3002 Size: 192 437 794 20 4672 Fragments arranged by size: 4672 794 437 192 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6115 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6080 DraI TTT'AAA Cuts at: 3419 3419 Size: 6115 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5122 809 Size: 507 3806 1802 Fragments arranged by size: 3806 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2501 2693 2735 Size: 54 1425 50 275 18 671 192 42 Cuts at: 2735 3562 4451 6080 8 Size: 827 889 1629 43 Fragments arranged by size: 1629 1425 889 827 671 275 192 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 2693 2735 3562 1537 Size: 275 881 42 827 4090 Fragments arranged by size: 4090 881 827 275 42 EarI CTCTTCn'nnn_ Cuts at: 1353 4045 1353 Size: 2692 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2191 3055 3059 4702 5076 5222 Size: 1064 138 602 864 4 1643 374 146 Cuts at: 5222 387 Size: 1280 Fragments arranged by size: 1643 1280 1064 864 602 374 146 138 4 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2076 3130 3197 3334 4178 4775 5562 Size: 393 444 1054 67 137 844 597 787 Cuts at: 5562 5830 1239 Size: 268 1524 Fragments arranged by size: 1524 1054 844 787 597 444 393 268 137 67 EcoO109I rG'GnC_Cy Cuts at: 2700 2701 3266 3267 2700 Size: 1 565 1 5548 Fragments arranged by size: 5548 565 1 1 EcoRI G'AATT_C Cuts at: 3424 3802 3424 Size: 378 5737 FspI TGC'GCA Cuts at: 2867 4621 2867 Size: 1754 4361 HaeII r_GCGC'y Cuts at: 1678 2189 2285 2294 2492 2564 2588 2747 5262 Size: 511 96 9 198 72 24 159 2515 Cuts at: 5262 1678 Size: 2531 Fragments arranged by size: 2531 2515 511 198 159 96 72 24 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2293 2325 2650 2682 2705 Size: 32 135 32 721 32 325 32 23 Cuts at: 2705 2737 4756 4788 4830 4862 1373 Size: 32 2019 32 42 32 2626 Fragments arranged by size: 2626 2019 721 325 135 42 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 3459 3705 669 Size: 988 281 1521 246 3079 Fragments arranged by size: 3079 1521 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6115 HpaI GTT'AAC Cuts at: 3705 3705 Size: 6115 KasI G'GCGC_C Cuts at: 2281 2290 2281 Size: 9 6106 KpnI G_GTAC'C Cuts at: 3440 3440 Size: 6115 MfeI C'AATT_G Cuts at: 3714 3714 Size: 6115 MluI A'CGCG_T Cuts at: 5991 5991 Size: 6115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5229 5413 1305 Size: 3924 184 2007 Fragments arranged by size: 3924 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4293 Fragments arranged by size: 4293 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2054 3251 4073 4432 4591 518 Size: 648 888 1197 822 359 159 2042 Fragments arranged by size: 2042 1197 888 822 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2199 2397 2425 2598 2783 2854 3531 4208 Size: 593 198 28 173 185 71 677 677 Cuts at: 4208 5356 5601 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 677 593 245 198 185 173 71 28 NaeI GCC'GGC Cuts at: 3042 3042 Size: 6115 NarI GG'CG_CC Cuts at: 2282 2291 2282 Size: 9 6106 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4624 Fragments arranged by size: 4624 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6115 NgoMIV G'CCGG_C Cuts at: 3040 3040 Size: 6115 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6115 NotI GC'GGCC_GC Cuts at: 3562 3562 Size: 6115 NspI r_CATG'y Cuts at: 1132 1229 2053 2254 2953 3473 5018 1132 Size: 97 824 201 699 520 1545 2229 Fragments arranged by size: 2229 1545 824 699 520 201 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6115 PciI A'CATG_T Cuts at: 5014 5014 Size: 6115 PflMI CCAn_nnn'nTGG Cuts at: 1108 2854 3108 1108 Size: 1746 254 4115 Fragments arranged by size: 4115 1746 254 PmeI GTTT'AAAC Cuts at: 3419 3419 Size: 6115 PmlI CAC'GTG Cuts at: 2476 2476 Size: 6115 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4184 4216 5697 5729 4184 Size: 32 1481 32 4570 Fragments arranged by size: 4570 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6115 PsiI TTA'TAA Cuts at: 3685 3685 Size: 6115 PspOMI G'GGCC_C Cuts at: 2700 3266 2700 Size: 566 5549 PstI C_TGCA'G Cuts at: 830 2958 3024 3189 3467 830 Size: 2128 66 165 278 3478 Fragments arranged by size: 3478 2128 278 165 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6115 PvuII CAG'CTG Cuts at: 2854 2854 Size: 6115 SacII CC_GC'GG Cuts at: 2426 2426 Size: 6115 SalI G'TCGA_C Cuts at: 1936 3457 1936 Size: 1521 4594 SbfI CC_TGCA'GG Cuts at: 3467 3467 Size: 6115 ScaI AGT'ACT Cuts at: 2816 4363 6075 2816 Size: 1547 1712 2856 Fragments arranged by size: 2856 1712 1547 SfcI C'TryA_G Cuts at: 826 1045 2245 2443 2954 3020 3185 3463 4598 Size: 219 1200 198 511 66 165 278 1135 Cuts at: 4598 5279 5470 826 Size: 681 191 1471 Fragments arranged by size: 1471 1200 1135 681 511 278 219 198 191 165 66 SfoI GGC'GCC Cuts at: 2283 2292 2283 Size: 9 6106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 SgrAI Cr'CCGG_yG Cuts at: 1700 3040 1700 Size: 1340 4775 SmaI CCC'GGG Cuts at: 1872 2146 2344 3442 1872 Size: 274 198 1098 4545 Fragments arranged by size: 4545 1098 274 198 SmlI C'TyrA_G Cuts at: 820 1017 1943 2747 2894 3146 4219 5120 5382 Size: 197 926 804 147 252 1073 901 262 Cuts at: 5382 820 Size: 1553 Fragments arranged by size: 1553 1073 926 901 804 262 252 197 147 SnaBI TAC'GTA Cuts at: 493 3906 493 Size: 3413 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6115 SphI G_CATG'C Cuts at: 1132 2254 2953 3473 1132 Size: 1122 699 520 3774 Fragments arranged by size: 3774 1122 699 520 SspI AAT'ATT Cuts at: 5 52 4039 5 Size: 47 3987 2081 Fragments arranged by size: 3987 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6115 StyI C'CwwG_G Cuts at: 513 1065 2004 3129 3262 3554 513 Size: 552 939 1125 133 292 3074 Fragments arranged by size: 3074 1125 939 552 292 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2238 4142 4159 4312 4497 4942 325 Size: 1255 658 1904 17 153 185 445 1498 Fragments arranged by size: 1904 1498 1255 658 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2814 3198 3404 Size: 274 80 33 51 1443 837 384 206 Cuts at: 3404 4361 6073 96 Size: 957 1712 138 Fragments arranged by size: 1712 1443 957 837 384 274 206 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6075 6107 6075 Size: 32 6083 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6115 XbaI T'CTAG_A Cuts at: 3451 3451 Size: 6115 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2511 2511 Size: 6115 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6115 XmaI C'CCGG_G Cuts at: 1870 2144 2342 3440 1870 Size: 274 198 1098 4545 Fragments arranged by size: 4545 1098 274 198 XmnI GAAnn'nnTTC Cuts at: 4244 4244 Size: 6115 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3923 276 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BfrBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI