Restriction map for pFN21AB7043
 (Linear) (Six-base) MAPSORT of: KIBB7043.seq  Check: 3739  from: 1  to: 1410



KIBB7043.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1329    1410 
   Size:       1329      81
 
AhdI GACnn_n'nnGTC

Cuts at:      0     302    1410 
   Size:        302    1108
 
AlwNI CAG_nnn'CTG

Cuts at:      0     831     849    1410 
   Size:        831      18     561

  Fragments arranged by size: 

                 831     561      18
 
ApaI G_GGCC'C

Cuts at:      0     699    1265    1410 
   Size:        699     566     145

  Fragments arranged by size: 

                 699     566     145
 
AvaI C'yCGr_G

Cuts at:      0     139     289     337     487    1410 
   Size:        139     150      48     150     923

  Fragments arranged by size: 

                 923     150     150     139      48
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     370     403    1104    1137    1410 
   Size:        370      33     701      33     273

  Fragments arranged by size: 

                 701     370     273      33      33
 
BanI G'GyrC_C

Cuts at:      0     276     285    1029    1053    1410 
   Size:        276       9     744      24     357

  Fragments arranged by size: 

                 744     357     276      24       9
 
BanII G_rGCy'C

Cuts at:      0     140     699     941    1265    1410 
   Size:        140     559     242     324     145

  Fragments arranged by size: 

                 559     324     242     145     140
 
BbeI G_GCGC'C

Cuts at:      0     280     289    1410 
   Size:        280       9    1121

  Fragments arranged by size: 

                1121     280       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     416     617     796    1410 
   Size:        416     201     179     614

  Fragments arranged by size: 

                 614     416     201     179
 
BbvCI CC'TCA_GC

Cuts at:      0     774    1410 
   Size:        774     636
 
Bme1580I G_kGCm'C

Cuts at:      0     650     699    1010    1058    1265    1410 
   Size:        650      49     311      48     207     145

  Fragments arranged by size: 

                 650     311     207     145      49      48
 
BmgBI CAC'GTC

Cuts at:      0    1170    1410 
   Size:       1170     240
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     789    1410 
   Size:        789     621
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71    1125    1192    1410 
   Size:         71    1054      67     218

  Fragments arranged by size: 

                1054     218      71      67
 
Bpu10I CC'TnA_GC

Cuts at:      0     774    1410 
   Size:        774     636
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     727     910    1126    1410 
   Size:        727     183     216     284

  Fragments arranged by size: 

                 727     284     216     183
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     973    1410 
   Size:        973     437
 
BsaAI yAC'GTr

Cuts at:      0     471    1410 
   Size:        471     939
 
BsaHI Gr'CG_yC

Cuts at:      0     277     286     874    1410 
   Size:        277       9     588     536

  Fragments arranged by size: 

                 588     536     277       9
 
BsaWI w'CCGG_w

Cuts at:      0     678    1410 
   Size:        678     732
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     697     705    1410 
   Size:        697       8     705

  Fragments arranged by size: 

                 705     697       8
 
BseYI C'CCAG_C

Cuts at:      0     162     300     311     869     940    1410 
   Size:        162     138      11     558      71     470

  Fragments arranged by size: 

                 558     470     162     138      71      11
 
BsiEI CG_ry'CG

Cuts at:      0     691     733    1410 
   Size:        691      42     677

  Fragments arranged by size: 

                 691     677      42
 
BsiHKAI G_wGCw'C

Cuts at:      0     658    1157    1410 
   Size:        658     499     253

  Fragments arranged by size: 

                 658     499     253
 
BsiWI C'GTAC_G

Cuts at:      0     269    1410 
   Size:        269    1141
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      85    1410 
   Size:         85    1325
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     650     658     699     941    1010    1058    1157 
   Size:        140     510       8      41     242      69      48      99

Cuts at:   1157    1265    1410 
   Size:        108     145

  Fragments arranged by size: 

                 510     242     145     140     108      99      69      48
                  41       8
 
BspEI T'CCGG_A

Cuts at:      0     678    1410 
   Size:        678     732
 
BsrBI CCG'CTC

Cuts at:      0     352     521     687    1410 
   Size:        352     169     166     723

  Fragments arranged by size: 

                 723     352     169     166
 
BsrDI GCAATG_nn'

Cuts at:      0    1328    1410 
   Size:       1328      82
 
BsrFI r'CCGG_y

Cuts at:      0     732    1035    1410 
   Size:        732     303     375

  Fragments arranged by size: 

                 732     375     303
 
BsrGI T'GTAC_A

Cuts at:      0    1399    1410 
   Size:       1399      11
 
BssHII G'CGCG_C

Cuts at:      0      69    1410 
   Size:         69    1341
 
BssSI C'ACGA_G

Cuts at:      0     651    1410 
   Size:        651     759
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     852    1410 
   Size:        852     558
 
BstYI r'GATC_y

Cuts at:      0     956    1410 
   Size:        956     454
 
Bsu36I CC'TnA_GG

Cuts at:      0      80    1410 
   Size:         80    1330
 
BtgI C'CryG_G

Cuts at:      0     220     374     418     575    1410 
   Size:        220     154      44     157     835

  Fragments arranged by size: 

                 835     220     157     154      44
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     146    1410 
   Size:        146    1264
 
BtsI GCAGTG_nn'

Cuts at:      0     997    1189    1410 
   Size:        997     192     221

  Fragments arranged by size: 

                 997     221     192
 
EaeI y'GGCC_r

Cuts at:      0     496     688     730    1410 
   Size:        496     192      42     680

  Fragments arranged by size: 

                 680     496     192      42
 
EagI C'GGCC_G

Cuts at:      0     688     730    1410 
   Size:        688      42     680

  Fragments arranged by size: 

                 688     680      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     186    1050    1054    1410 
   Size:        186     864       4     356

  Fragments arranged by size: 

                 864     356     186       4
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71    1125    1192    1329    1410 
   Size:         71    1054      67     137      81

  Fragments arranged by size: 

                1054     137      81      71      67
 
EcoO109I rG'GnC_Cy

Cuts at:      0     695     696    1261    1262    1410 
   Size:        695       1     565       1     148

  Fragments arranged by size: 

                 695     565     148       1       1
 
FspI TGC'GCA

Cuts at:      0     862    1410 
   Size:        862     548
 
HaeII r_GCGC'y

Cuts at:      0     184     280     289     487     559     583     742    1410 
   Size:        184      96       9     198      72      24     159     668

  Fragments arranged by size: 

                 668     198     184     159      96      72      24       9
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     288     320     645     677     700     732    1410 
   Size:        288      32     325      32      23      32     678

  Fragments arranged by size: 

                 678     325     288      32      32      32      23
 
KasI G'GCGC_C

Cuts at:      0     276     285    1410 
   Size:        276       9    1125

  Fragments arranged by size: 

                1125     276       9
 
MslI CAynn'nnrTG

Cuts at:      0      49    1246    1410 
   Size:         49    1197     164

  Fragments arranged by size: 

                1197     164      49
 
MspA1I CmG'CkG

Cuts at:      0     194     392     420     593     778     849    1410 
   Size:        194     198      28     173     185      71     561

  Fragments arranged by size: 

                 561     198     194     185     173      71      28
 
NaeI GCC'GGC

Cuts at:      0    1037    1410 
   Size:       1037     373
 
NarI GG'CG_CC

Cuts at:      0     277     286    1410 
   Size:        277       9    1124

  Fragments arranged by size: 

                1124     277       9
 
NgoMIV G'CCGG_C

Cuts at:      0    1035    1410 
   Size:       1035     375
 
NspI r_CATG'y

Cuts at:      0      48     249     948    1410 
   Size:         48     201     699     462

  Fragments arranged by size: 

                 699     462     201      48
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     849    1103    1410 
   Size:        849     254     307

  Fragments arranged by size: 

                 849     307     254
 
PmlI CAC'GTG

Cuts at:      0     471    1410 
   Size:        471     939
 
PspOMI G'GGCC_C

Cuts at:      0     695    1261    1410 
   Size:        695     566     149

  Fragments arranged by size: 

                 695     566     149
 
PstI C_TGCA'G

Cuts at:      0     953    1019    1184    1410 
   Size:        953      66     165     226

  Fragments arranged by size: 

                 953     226     165      66
 
PvuII CAG'CTG

Cuts at:      0     849    1410 
   Size:        849     561
 
SacII CC_GC'GG

Cuts at:      0     421    1410 
   Size:        421     989
 
ScaI AGT'ACT

Cuts at:      0     811    1410 
   Size:        811     599
 
SfcI C'TryA_G

Cuts at:      0     240     438     949    1015    1180    1410 
   Size:        240     198     511      66     165     230

  Fragments arranged by size: 

                 511     240     230     198     165      66
 
SfoI GGC'GCC

Cuts at:      0     278     287    1410 
   Size:        278       9    1123

  Fragments arranged by size: 

                1123     278       9
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1035    1410 
   Size:       1035     375
 
SmaI CCC'GGG

Cuts at:      0     141     339    1410 
   Size:        141     198    1071

  Fragments arranged by size: 

                1071     198     141
 
SmlI C'TyrA_G

Cuts at:      0     742     889    1141    1410 
   Size:        742     147     252     269

  Fragments arranged by size: 

                 742     269     252     147
 
SphI G_CATG'C

Cuts at:      0     249     948    1410 
   Size:        249     699     462

  Fragments arranged by size: 

                 699     462     249
 
StyI C'CwwG_G

Cuts at:      0    1124    1257    1410 
   Size:       1124     133     153

  Fragments arranged by size: 

                1124     153     133
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     233    1410 
   Size:        233    1177
 
TatI w'GTAC_w

Cuts at:      0     809    1193    1399    1410 
   Size:        809     384     206      11

  Fragments arranged by size: 

                 809     384     206      11
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     506    1410 
   Size:        506     904
 
XmaI C'CCGG_G

Cuts at:      0     139     337    1410 
   Size:        139     198    1073

  Fragments arranged by size: 

                1073     198     139
 

 Enzymes that do cut:

     AcuI     AhdI    AlwNI     ApaI     AvaI     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI Bme1580I    BmgBI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     EaeI     EagI     EciI  Eco57MI EcoO109I     FspI    HaeII
    Hin4I     KasI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
    PflMI     PmlI   PspOMI     PstI    PvuII    SacII     ScaI     SfcI
     SfoI    SgrAI     SmaI     SmlI     SphI     StyI    TaqII     TatI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII    BamHI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI     BmrI     BmtI    BsaBI    BsaXI     BsgI     BsmI
    BspHI    BspMI    BstBI   BstEII    BstXI  BstZ17I     ClaI    CspCI
     DraI   DraIII     DrdI     EarI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SgfI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmnI     ZraI
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