(Circular) (Six-base) MAPSORT of: pFN21AB5636.seq Check: 9829 from: 1 to: 5698 pFN21AB5636 5698 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3041 1937 Size: 1104 4594 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3761 5145 5413 1632 Size: 2129 1384 268 1917 Fragments arranged by size: 2129 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 4597 5574 1203 Size: 3394 977 1327 Fragments arranged by size: 3394 1327 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AlwNI CAG_nnn'CTG Cuts at: 2434 3441 5013 2434 Size: 1007 1572 3119 Fragments arranged by size: 3119 1572 1007 ApaI G_GGCC'C Cuts at: 2301 2301 Size: 5698 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 3007 3233 3385 5494 1493 Size: 1514 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 226 152 AscI GG'CGCG_CC Cuts at: 2826 2826 Size: 5698 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 2182 2294 2994 3023 1870 Size: 73 239 112 700 29 4545 Fragments arranged by size: 4545 700 239 112 73 29 BamHI G'GATC_C Cuts at: 3028 3395 3028 Size: 367 5331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2210 2266 2753 3019 4474 Size: 325 217 627 423 56 487 266 1455 Cuts at: 4474 618 Size: 1842 Fragments arranged by size: 1842 1455 627 487 423 325 266 217 56 BanII G_rGCy'C Cuts at: 1391 2151 2301 2634 2928 3017 1391 Size: 760 150 333 294 89 4072 Fragments arranged by size: 4072 760 333 294 150 89 BbeI G_GCGC'C Cuts at: 2214 2214 Size: 5698 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4727 BbvCI CC'TCA_GC Cuts at: 2423 2423 Size: 5698 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2081 2187 4309 Size: 107 122 71 631 605 409 106 2122 Cuts at: 4309 136 Size: 1525 Fragments arranged by size: 2122 1525 631 605 409 122 107 106 71 BglII A'GATC_T Cuts at: 5693 5693 Size: 5698 BlpI GC'TnA_GC Cuts at: 2672 3115 2672 Size: 443 5255 Bme1580I G_kGCm'C Cuts at: 1790 2271 2301 2756 3759 4915 1790 Size: 481 30 455 1003 1156 2573 Fragments arranged by size: 2573 1156 1003 481 455 30 BmgBI CAC'GTC Cuts at: 1318 2854 1318 Size: 1536 4162 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2147 2174 3429 Size: 416 53 83 399 24 730 27 1255 Cuts at: 3429 4387 442 Size: 958 1753 Fragments arranged by size: 1753 1255 958 730 416 399 83 53 27 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2854 4358 1239 Size: 1615 1504 2579 Fragments arranged by size: 2579 1615 1504 Bpu10I CC'TnA_GC Cuts at: 2423 2423 Size: 5698 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3823 4688 4986 3823 Size: 865 298 4535 Fragments arranged by size: 4535 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2506 4361 882 Size: 617 1007 1855 2219 Fragments arranged by size: 2219 1855 1007 617 BsaAI yAC'GTr Cuts at: 493 1204 3489 493 Size: 711 2285 2702 Fragments arranged by size: 2702 2285 711 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2112 2211 3505 3887 Size: 53 83 186 936 579 99 1294 382 Cuts at: 3887 4522 275 Size: 635 1451 Fragments arranged by size: 1451 1294 936 635 579 382 186 99 83 53 BsaWI w'CCGG_w Cuts at: 4131 4529 4803 4950 5361 4131 Size: 398 274 147 411 4468 Fragments arranged by size: 4468 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5473 Fragments arranged by size: 5473 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2524 2759 2762 2936 1186 Size: 1338 235 3 174 3948 Fragments arranged by size: 3948 1338 235 174 3 BseYI C'CCAG_C Cuts at: 2046 2757 4901 5397 2046 Size: 711 2144 496 2347 Fragments arranged by size: 2347 2144 711 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2305 2381 1163 Size: 573 569 76 4480 Fragments arranged by size: 4480 573 569 76 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2018 2070 2139 2250 2353 2404 Size: 275 186 17 52 69 111 103 51 Cuts at: 2404 3148 3909 4937 1540 Size: 744 761 1028 2301 Fragments arranged by size: 2301 1028 761 744 275 186 111 103 69 52 51 17 BsiHKAI G_wGCw'C Cuts at: 2634 3759 3844 4915 2634 Size: 1125 85 1071 3417 Fragments arranged by size: 3417 1125 1071 85 BsmI GAATG_Cn' Cuts at: 3209 3302 3209 Size: 93 5605 BsmBI CGTCTCn'nnnn_ Cuts at: 2117 2117 Size: 5698 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2271 2301 2634 2756 2928 3017 Size: 399 361 120 30 333 122 172 89 Cuts at: 3017 3759 3844 4915 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 361 333 172 122 120 89 85 30 BspEI T'CCGG_A Cuts at: 5361 5361 Size: 5698 BspHI T'CATG_A Cuts at: 3587 5317 3587 Size: 1730 3968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3053 844 Size: 2209 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2014 3585 1394 Size: 429 191 1571 3507 Fragments arranged by size: 3507 1571 429 191 BsrDI GCAATG_nn' Cuts at: 65 1248 4193 4367 65 Size: 1183 2945 174 1396 Fragments arranged by size: 2945 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2198 2987 4342 4529 1700 Size: 110 388 789 1355 187 2869 Fragments arranged by size: 2869 1355 789 388 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5698 BssHII G'CGCG_C Cuts at: 1925 2169 2171 2255 2406 2826 1925 Size: 244 2 84 151 420 4797 Fragments arranged by size: 4797 420 244 151 84 2 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 2854 794 Size: 2060 3638 BstBI TT'CG_AA Cuts at: 4502 4502 Size: 5698 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5698 BstYI r'GATC_y Cuts at: 1972 2685 3028 3395 3780 3797 5249 5335 5347 Size: 713 343 367 385 17 1452 86 12 Cuts at: 5347 5693 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 713 385 367 346 343 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2608 487 Size: 683 56 1382 3577 Fragments arranged by size: 3577 1382 683 56 BtsI GCAGTG_nn' Cuts at: 2044 3214 4008 4028 2044 Size: 1170 794 20 3714 Fragments arranged by size: 3714 1170 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2015 2067 2136 Size: 54 1425 50 275 18 185 52 69 Cuts at: 2136 2247 2350 2401 2697 2721 3145 4034 5663 Size: 111 103 51 296 24 424 889 1629 Cuts at: 5663 8 Size: 43 Fragments arranged by size: 1629 1425 889 424 296 275 185 111 103 69 54 52 51 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2015 2067 2136 2247 2350 2401 3145 Size: 275 203 52 69 111 103 51 744 Cuts at: 3145 1537 Size: 4090 Fragments arranged by size: 4090 744 275 203 111 103 69 52 51 EarI CTCTTCn'nnn_ Cuts at: 1353 2501 3628 1353 Size: 1148 1127 3423 Fragments arranged by size: 3423 1148 1127 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2681 4285 4659 4805 387 Size: 1064 138 1092 1604 374 146 1280 Fragments arranged by size: 1604 1280 1092 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2632 2632 Size: 5698 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2854 3761 4358 5145 5413 1239 Size: 393 1222 907 597 787 268 1524 Fragments arranged by size: 1524 1222 907 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2619 2779 2619 Size: 160 5538 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2873 2873 Size: 5698 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 2214 4845 1678 Size: 536 2631 2531 Fragments arranged by size: 2631 2531 536 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4339 4371 4413 4445 1373 Size: 32 135 32 2767 32 42 32 2626 Fragments arranged by size: 2767 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3042 3288 669 Size: 988 281 1104 246 3079 Fragments arranged by size: 3079 1104 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5698 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KasI G'GCGC_C Cuts at: 2210 2210 Size: 5698 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4812 4996 1305 Size: 3507 184 2007 Fragments arranged by size: 3507 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2699 2723 10 Size: 54 1425 343 867 24 2985 Fragments arranged by size: 2985 1425 867 343 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 3656 4015 4174 518 Size: 648 2490 359 159 2042 Fragments arranged by size: 2490 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2144 2333 2365 2434 2451 2761 2874 3114 Size: 538 189 32 69 17 310 113 240 Cuts at: 3114 3791 4939 5184 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 538 310 245 240 189 113 69 32 17 NaeI GCC'GGC Cuts at: 2989 2989 Size: 5698 NarI GG'CG_CC Cuts at: 2211 2211 Size: 5698 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5146 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NgoMIV G'CCGG_C Cuts at: 2987 2987 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 2015 2136 3145 2015 Size: 121 1009 4568 Fragments arranged by size: 4568 1009 121 NspI r_CATG'y Cuts at: 1132 1229 3056 4601 1132 Size: 97 1827 1545 2229 Fragments arranged by size: 2229 1827 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5698 PciI A'CATG_T Cuts at: 4597 4597 Size: 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 2434 1108 Size: 1326 4372 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2779 2779 Size: 5698 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PspOMI G'GGCC_C Cuts at: 2297 2297 Size: 5698 PstI C_TGCA'G Cuts at: 830 3050 830 Size: 2220 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 PvuII CAG'CTG Cuts at: 2365 2874 2365 Size: 509 5189 SacI G_AGCT'C Cuts at: 2634 2634 Size: 5698 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SexAI A'CCwGG_T Cuts at: 2094 2094 Size: 5698 SfcI C'TryA_G Cuts at: 826 1045 2416 3046 4181 4862 5053 826 Size: 219 1371 630 1135 681 191 1471 Fragments arranged by size: 1471 1371 1135 681 630 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2081 2081 Size: 5698 SfoI GGC'GCC Cuts at: 2212 2212 Size: 5698 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 2184 2296 3025 1872 Size: 312 112 729 4545 Fragments arranged by size: 4545 729 312 112 SmlI C'TyrA_G Cuts at: 820 1017 1943 3802 4703 4965 820 Size: 197 926 1859 901 262 1553 Fragments arranged by size: 1859 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3489 493 Size: 2996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5698 StyI C'CwwG_G Cuts at: 513 1065 2118 3137 513 Size: 552 1053 1019 3074 Fragments arranged by size: 3074 1053 1019 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 2771 3725 3742 3895 4080 4525 Size: 1255 444 747 954 17 153 185 445 Cuts at: 4525 325 Size: 1498 Fragments arranged by size: 1498 1255 954 747 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3944 5656 96 Size: 274 80 33 51 1443 1967 1712 138 Fragments arranged by size: 1967 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5658 5690 5658 Size: 32 5666 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5698 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 2182 2294 3023 1870 Size: 312 112 729 4545 Fragments arranged by size: 4545 729 312 112 XmnI GAAnn'nnTTC Cuts at: 3827 3827 Size: 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SrfI SwaI XcmI