(Circular) (Six-base) MAPSORT of: pFN21AB5582.seq Check: 5848 from: 1 to: 5827 pFN21AB5582 5827 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3637 278 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3170 1937 Size: 1233 4594 Acc65I G'GTAC_C Cuts at: 2381 3148 2381 Size: 767 5060 AclI AA'CG_TT Cuts at: 1563 3954 4327 1563 Size: 2391 373 3063 Fragments arranged by size: 3063 2391 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 2877 3890 5274 5542 1632 Size: 641 604 1013 1384 268 1917 Fragments arranged by size: 1917 1384 1013 641 604 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5630 AflIII A'CryG_T Cuts at: 1203 4726 5703 1203 Size: 3523 977 1327 Fragments arranged by size: 3523 1327 977 AgeI A'CCGG_T Cuts at: 2537 4658 2537 Size: 2121 3706 AhdI GACnn_n'nnGTC Cuts at: 4556 4556 Size: 5827 AleI CACnn'nnGTG Cuts at: 1166 2456 1166 Size: 1290 4537 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5827 AlwNI CAG_nnn'CTG Cuts at: 3570 5142 3570 Size: 1572 4255 ApaLI G'TGCA_C Cuts at: 3884 5040 3884 Size: 1156 4671 ApoI r'AATT_y Cuts at: 1493 2306 2687 3035 3136 3362 3514 5623 1493 Size: 813 381 348 101 226 152 2109 1697 Fragments arranged by size: 2109 1697 813 381 348 226 152 101 AseI AT'TA_AT Cuts at: 160 1032 2369 4381 160 Size: 872 1337 2012 1606 Fragments arranged by size: 2012 1606 1337 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 AvaI C'yCGr_G Cuts at: 1870 1943 3152 1870 Size: 73 1209 4545 Fragments arranged by size: 4545 1209 73 BamHI G'GATC_C Cuts at: 3157 3524 3157 Size: 367 5460 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2086 2381 3148 4603 618 Size: 325 217 627 299 295 767 1455 1842 Fragments arranged by size: 1842 1455 767 627 325 299 295 217 BanII G_rGCy'C Cuts at: 1391 3146 1391 Size: 1755 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4856 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4018 4052 1903 Size: 34 2081 34 3678 Fragments arranged by size: 3678 2081 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3719 4929 3719 Size: 1210 4617 BclI T'GATC_A Cuts at: 1548 1548 Size: 5827 BfrBI ATG'CAT Cuts at: 3091 3091 Size: 5827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4438 136 Size: 107 122 71 631 605 2766 1525 Fragments arranged by size: 2766 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5822 5822 Size: 5827 BlpI GC'TnA_GC Cuts at: 3244 3244 Size: 5827 Bme1580I G_kGCm'C Cuts at: 1790 3888 5044 1790 Size: 2098 1156 2573 Fragments arranged by size: 2573 2098 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5827 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3558 4516 442 Size: 416 53 83 399 24 2141 958 1753 Fragments arranged by size: 2141 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5827 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2596 2596 Size: 5827 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4487 1239 Size: 3248 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2340 2861 3952 4817 5115 2340 Size: 521 1091 865 298 3052 Fragments arranged by size: 3052 1091 865 521 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4490 882 Size: 617 2991 2219 Fragments arranged by size: 2991 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3618 493 Size: 711 2414 2702 Fragments arranged by size: 2702 2414 711 BsaBI GATnn'nnATC Cuts at: 5821 5821 Size: 5827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2865 3634 4016 4651 Size: 53 83 186 936 1332 769 382 635 Cuts at: 4651 275 Size: 1451 Fragments arranged by size: 1451 1332 936 769 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2537 4260 4658 4932 5079 5490 2537 Size: 1723 398 274 147 411 2874 Fragments arranged by size: 2874 1723 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5602 Fragments arranged by size: 5602 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2622 2799 1186 Size: 901 535 177 4214 Fragments arranged by size: 4214 901 535 177 BseYI C'CCAG_C Cuts at: 2859 5030 5526 2859 Size: 2171 496 3160 Fragments arranged by size: 3160 2171 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5254 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3277 4038 5066 1540 Size: 275 186 1276 761 1028 2301 Fragments arranged by size: 2301 1276 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2766 3888 3973 5044 2766 Size: 1122 85 1071 3549 Fragments arranged by size: 3549 1122 1071 85 BsmI GAATG_Cn' Cuts at: 2306 3091 3105 3338 3431 2306 Size: 785 14 233 93 4702 Fragments arranged by size: 4702 785 233 93 14 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2766 3146 3888 3973 5044 1391 Size: 399 976 380 742 85 1071 2174 Fragments arranged by size: 2174 1071 976 742 399 380 85 BspEI T'CCGG_A Cuts at: 5490 5490 Size: 5827 BspHI T'CATG_A Cuts at: 2101 2826 3716 5446 2101 Size: 725 890 1730 2482 Fragments arranged by size: 2482 1730 890 725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3182 844 Size: 2338 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3714 1394 Size: 429 1891 3507 Fragments arranged by size: 3507 1891 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2121 4322 4496 65 Size: 1183 873 2201 174 1396 Fragments arranged by size: 2201 1396 1183 873 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2537 4471 4658 1700 Size: 110 727 1934 187 2869 Fragments arranged by size: 2869 1934 727 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5827 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5827 BssSI C'ACGA_G Cuts at: 3887 4899 3887 Size: 1012 4815 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5827 BstBI TT'CG_AA Cuts at: 2110 3109 4631 2110 Size: 999 1522 3306 Fragments arranged by size: 3306 1522 999 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5827 BstYI r'GATC_y Cuts at: 1972 2485 3157 3524 3909 3926 5378 5464 5476 Size: 513 672 367 385 17 1452 86 12 Cuts at: 5476 5822 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 672 513 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2175 2733 2175 Size: 558 5269 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4336 Fragments arranged by size: 4336 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5088 Fragments arranged by size: 5088 683 56 BtsI GCAGTG_nn' Cuts at: 2127 2559 2952 3343 4137 4157 2127 Size: 432 393 391 794 20 3797 Fragments arranged by size: 3797 794 432 393 391 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5792 DraI TTT'AAA Cuts at: 3131 3131 Size: 5827 DraIII CAC_nnn'GTG Cuts at: 2294 2294 Size: 5827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4834 809 Size: 507 3518 1802 Fragments arranged by size: 3518 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3274 4163 5792 Size: 54 1425 50 275 18 1444 889 1629 Cuts at: 5792 8 Size: 43 Fragments arranged by size: 1629 1444 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3274 1537 Size: 275 1462 4090 Fragments arranged by size: 4090 1462 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2966 3757 1353 Size: 1613 791 3423 Fragments arranged by size: 3423 1613 791 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4414 4788 4934 387 Size: 1064 138 2825 374 146 1280 Fragments arranged by size: 2825 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2877 3890 4487 5274 5542 1239 Size: 393 641 604 1013 597 787 268 1524 Fragments arranged by size: 1524 1013 787 641 604 597 393 268 EcoRI G'AATT_C Cuts at: 3136 3514 3136 Size: 378 5449 EcoRV GAT'ATC Cuts at: 2467 2467 Size: 5827 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2531 2531 Size: 5827 FspI TGC'GCA Cuts at: 4333 4333 Size: 5827 HaeII r_GCGC'y Cuts at: 1678 4974 1678 Size: 3296 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3034 3066 4468 4500 4542 Size: 32 135 32 1462 32 1402 32 42 Cuts at: 4542 4574 1373 Size: 32 2626 Fragments arranged by size: 2626 1462 1402 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3171 3417 669 Size: 988 281 1233 246 3079 Fragments arranged by size: 3079 1233 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5827 HpaI GTT'AAC Cuts at: 3417 3417 Size: 5827 KpnI G_GTAC'C Cuts at: 2385 3152 2385 Size: 767 5060 MfeI C'AATT_G Cuts at: 3426 3426 Size: 5827 MluI A'CGCG_T Cuts at: 5703 5703 Size: 5827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2550 2593 2860 4941 5125 1305 Size: 1245 43 267 2081 184 2007 Fragments arranged by size: 2081 2007 1245 267 184 43 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4005 Fragments arranged by size: 4005 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2145 2208 2456 2666 2771 3785 4144 Size: 648 979 63 248 210 105 1014 359 Cuts at: 4144 4303 518 Size: 159 2042 Fragments arranged by size: 2042 1014 979 648 359 248 210 159 105 63 MspA1I CmG'CkG Cuts at: 1606 2926 3243 3920 5068 5313 1606 Size: 1320 317 677 1148 245 2120 Fragments arranged by size: 2120 1320 1148 677 317 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4336 Fragments arranged by size: 4336 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5827 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5827 NotI GC'GGCC_GC Cuts at: 3274 3274 Size: 5827 NsiI A_TGCA'T Cuts at: 3093 3093 Size: 5827 NspI r_CATG'y Cuts at: 1132 1229 2850 3185 4730 1132 Size: 97 1621 335 1545 2229 Fragments arranged by size: 2229 1621 1545 335 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5827 PciI A'CATG_T Cuts at: 4726 4726 Size: 5827 PflMI CCAn_nnn'nTGG Cuts at: 1108 2294 1108 Size: 1186 4641 PfoI T'CCnGG_A Cuts at: 2654 2754 2654 Size: 100 5727 PmeI GTTT'AAAC Cuts at: 3131 3131 Size: 5827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 5409 5441 3896 Size: 32 1481 32 4282 Fragments arranged by size: 4282 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5827 PsiI TTA'TAA Cuts at: 3397 3397 Size: 5827 PstI C_TGCA'G Cuts at: 830 2038 3179 830 Size: 1208 1141 3478 Fragments arranged by size: 3478 1208 1141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5827 PvuII CAG'CTG Cuts at: 2926 2926 Size: 5827 SalI G'TCGA_C Cuts at: 1936 3169 1936 Size: 1233 4594 SbfI CC_TGCA'GG Cuts at: 3179 3179 Size: 5827 ScaI AGT'ACT Cuts at: 4075 5787 4075 Size: 1712 4115 SfcI C'TryA_G Cuts at: 826 1045 2034 3175 4310 4991 5182 826 Size: 219 989 1141 1135 681 191 1471 Fragments arranged by size: 1471 1141 1135 989 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5827 SmaI CCC'GGG Cuts at: 1872 3154 1872 Size: 1282 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2355 2840 3931 4832 5094 820 Size: 197 926 412 485 1091 901 262 1553 Fragments arranged by size: 1553 1091 926 901 485 412 262 197 SnaBI TAC'GTA Cuts at: 493 3618 493 Size: 3125 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5827 SphI G_CATG'C Cuts at: 1132 3185 1132 Size: 2053 3774 SspI AAT'ATT Cuts at: 5 52 3751 5 Size: 47 3699 2081 Fragments arranged by size: 3699 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5827 StyI C'CwwG_G Cuts at: 513 1065 2004 3266 513 Size: 552 939 1262 3074 Fragments arranged by size: 3074 1262 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2792 3854 3871 4024 4209 4654 325 Size: 1255 1212 1062 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1212 1062 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4073 5785 96 Size: 274 80 33 51 1443 2096 1712 138 Fragments arranged by size: 2096 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2268 2300 2650 2682 5787 5819 2268 Size: 32 350 32 3105 32 2276 Fragments arranged by size: 3105 2276 350 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2267 1316 Size: 951 4876 XbaI T'CTAG_A Cuts at: 3163 3163 Size: 5827 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5827 XmaI C'CCGG_G Cuts at: 1870 3152 1870 Size: 1282 4545 XmnI GAAnn'nnTTC Cuts at: 3956 3956 Size: 5827 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3635 276 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI