Restriction map for
pFN21AB5574
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BanI BanII BbeI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoO109I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
MaeI MamI MfeI MflI MluI
MroI MseI MslI MunI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 250 GTCTAC GT^[AC][GT]AC
AfaI 111 GTAC GT^AC
Alw44I 115 GTGCAC G^TGCAC
AlwI 237 GATCC GGATC(4/5)
AlwNI 542 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 115 GTGCAC G^TGCAC
AspHI 115 GTGCAC G[AT]GC[AT]^C
AviII 470 TGCGCA TGC^GCA
BalI 330 TGGCCA TGG^CCA
BbrPI 354 CACGTG CAC^GTG
BbsI 25 GTCTTC GAAGAC(2/6)
BbvCI 190 GCTGAGG CCTCAGC(-5/-2)
BmyI 115 GTGCAC G[AGT]GC[ACT]^C
BpuAI 25 GTCTTC GAAGAC(2/6)
BseRI 18 CTCCTC GAGGAG(10/8)
BsiEI 591 CGGCCG CG[GA][CT]^CG
BsiHKAI 115 GTGCAC G[AT]GC[AT]^C
BsmAI 498 GAGAC GTCTC(1/5)
BsmBI 498 GAGACG CGTCTC(1/5)
Bsp1286I 115 GTGCAC G[AGT]GC[ACT]^C
BsrI 563 ACTGG ACTGG(1/-1)
BssSI 445 CTCGTG CACGAG(-5/-1)
BstEII 303 GGTGACC G^GT[ACGT]ACC
BstF5I 508 GGATG GGATG(2/0)
BstPI 303 GGTGACC G^GT[ACGT]ACC
BstXI 92 CCAAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 591 CGGCCG C^GGCCG
EclXI 591 CGGCCG C^GGCCG
Eco52I 591 CGGCCG C^GGCCG
EcoNI 186 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 303 GGTGACC G^GT[ACGT]ACC
FauI 391 GCGGG CCCGC(4/6)
FokI 508 GGATG GGATG(9/13)
FspI 470 TGCGCA TGC^GCA
HphI 303 GGTGA GGTGA(8/7)
KspI 389 CCGCGG CCGC^GG
MaeIII 304 GTGAC ^GT[ACGT]AC
MluNI 330 TGGCCA TGG^CCA
MscI 330 TGGCCA TGG^CCA
NaeI 136 GCCGGC GCC^GGC
NcoI 431 CCATGG C^CATGG
NgoMIV 136 GCCGGC G^CCGGC
NlaIV 174 GGTCCC GG[ACGT]^[ACGT]CC
PflMI 561 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 354 CACGTG CAC^GTG
PmlI 354 CACGTG CAC^GTG
PshAI 513 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 271 CAGCTG CAG^CTG
RsaI 111 GTAC GT^AC
SacII 389 CCGCGG CCGC^GG
SmaI 177 CCCGGG CCC^GGG
SmlI 523 CTCAAG C^T[CT][GA]AG
Tsp45I 304 GTGAC ^GT[GC]AC
TspRI 562 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 561 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 177 CCCGGG C^CCGGG
XmaI 177 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 46 GACGCC G[GA]^CG[CT]C
182 GACGCC
AvaI 157 CCCGAG C^[CT]CG[GA]G
177 CCCGGG
AvaII 174 GGTCC G^G[AT]CC
435 GGTCC
BpmI 147 CTGGAG CTGGAG(16/14)
229 CTGGAG
BsaAI 112 TACGTG [CT]AC^GT[GA]
354 CACGTG
BsaHI 46 GACGCC G[GA]^CG[CT]C
182 GACGCC
BsgI 274 CTGCAC GTGCAG(16/14)
575 CTGCAC
BsoBI 157 CCCGAG C^[CT]CG[GA]G
177 CCCGGG
BsrBI 57 CCGCTC CCGCTC(-3/-3)
555 CCGCTC
BsrFI 136 GCCGGC [GA]^CCGG[CT]
171 GCCGGT
BssHII 132 GCGCGC G^CGCGC
152 GCGCGC
Cfr10I 136 GCCGGC [GA]^CCGG[CT]
171 GCCGGT
DdeI 191 CTGAG C^T[ACGT]AG
293 CTGAG
DpnII 39 GATC ^GATC
237 GATC
EcoT14I 92 CCAAGG C^C[AT][AT]GG
431 CCATGG
HaeII 14 GGCGCT [GA]GCGC^[CT]
96 GGCGCT
Hin1I 46 GACGCC G[GA]^CG[CT]C
182 GACGCC
MboI 39 GATC ^GATC
237 GATC
MspA1I 271 CAGCTG C[AC]G^C[GT]G
389 CCGCGG
NdeII 39 GATC ^GATC
237 GATC
NlaIII 42 CATG CATG^
432 CATG
Sau3AI 39 GATC ^GATC
237 GATC
SfaNI 310 GATGC GCATC(5/9)
350 GCATC
StyI 92 CCAAGG C^C[AT][AT]GG
431 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BsaJI BsiYI BslI BsmFI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DsaI EaeI EcoRII Fnu4HI HaeIII
HapII HgaI HhaI HinP1I HpaII
ItaI MaeII MboII MnlI MspI
MvaI MvnI MwoI NciI PstI
Sau96I ScrFI SfcI TseI