(Circular) (Six-base) MAPSORT of: pFN21AB5497.seq Check: 6275 from: 1 to: 6142 pFN21AB5497 6142 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3485 1937 Size: 1548 4594 Acc65I G'GTAC_C Cuts at: 3463 3463 Size: 6142 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 3367 4205 5589 5857 1632 Size: 440 1295 838 1384 268 1917 Fragments arranged by size: 1917 1384 1295 838 440 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 5041 6018 1203 Size: 3838 977 1327 Fragments arranged by size: 3838 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6142 AlwNI CAG_nnn'CTG Cuts at: 2275 2302 3885 5457 2275 Size: 27 1583 1572 2960 Fragments arranged by size: 2960 1583 1572 27 ApaLI G'TGCA_C Cuts at: 4199 5355 4199 Size: 1156 4986 ApoI r'AATT_y Cuts at: 1493 2997 3043 3081 3249 3451 3677 3829 5938 Size: 1504 46 38 168 202 226 152 2109 Cuts at: 5938 1493 Size: 1697 Fragments arranged by size: 2109 1697 1504 226 202 168 152 46 38 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 2199 2249 2331 3467 1870 Size: 73 256 50 82 1136 4545 Fragments arranged by size: 4545 1136 256 82 73 50 BamHI G'GATC_C Cuts at: 3472 3839 3472 Size: 367 5775 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3463 4918 618 Size: 325 217 627 1676 1455 1842 Fragments arranged by size: 1842 1676 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2229 2325 2654 3102 3461 1391 Size: 838 96 329 448 359 4072 Fragments arranged by size: 4072 838 448 359 329 96 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5171 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4333 4367 1903 Size: 34 2396 34 3678 Fragments arranged by size: 3678 2396 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3038 4034 5244 3038 Size: 996 1210 3936 Fragments arranged by size: 3936 1210 996 BclI T'GATC_A Cuts at: 1548 2304 1548 Size: 756 5386 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4753 136 Size: 107 122 71 631 605 3081 1525 Fragments arranged by size: 3081 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6137 6137 Size: 6142 BlpI GC'TnA_GC Cuts at: 3321 3559 3321 Size: 238 5904 Bme1580I G_kGCm'C Cuts at: 1790 2029 4203 5359 1790 Size: 239 2174 1156 2573 Fragments arranged by size: 2573 2174 1156 239 BmgBI CAC'GTC Cuts at: 1318 2368 1318 Size: 1050 5092 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3873 4831 442 Size: 416 53 83 399 24 2456 958 1753 Fragments arranged by size: 2456 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2297 2309 2312 2402 3027 3279 4802 1239 Size: 1058 12 3 90 625 252 1523 2579 Fragments arranged by size: 2579 1523 1058 625 252 90 12 3 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4267 5132 5430 4267 Size: 865 298 4979 Fragments arranged by size: 4979 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2180 2246 4805 882 Size: 617 681 66 2559 2219 Fragments arranged by size: 2559 2219 681 617 66 BsaAI yAC'GTr Cuts at: 493 1204 3933 493 Size: 711 2729 2702 Fragments arranged by size: 2729 2702 711 BsaBI GATnn'nnATC Cuts at: 6136 6136 Size: 6142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3949 4331 4966 275 Size: 53 83 186 936 2416 382 635 1451 Fragments arranged by size: 2416 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4575 4973 5247 5394 5805 4575 Size: 398 274 147 411 4912 Fragments arranged by size: 4912 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2138 2168 1178 Size: 30 165 30 735 30 5152 Fragments arranged by size: 5152 735 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2477 2561 2899 3110 3137 1186 Size: 1291 84 338 211 27 4191 Fragments arranged by size: 4191 1291 338 211 84 27 BseYI C'CCAG_C Cuts at: 5345 5841 5345 Size: 496 5646 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2357 1163 Size: 573 621 4948 Fragments arranged by size: 4948 621 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3592 4353 5381 1540 Size: 275 186 1591 761 1028 2301 Fragments arranged by size: 2301 1591 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2229 2325 4203 4288 5359 2229 Size: 96 1878 85 1071 3012 Fragments arranged by size: 3012 1878 1071 96 85 BsmI GAATG_Cn' Cuts at: 2977 3653 3746 2977 Size: 676 93 5373 Fragments arranged by size: 5373 676 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2229 2325 2654 3102 3461 4203 Size: 399 239 200 96 329 448 359 742 Cuts at: 4203 4288 5359 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 448 399 359 329 239 200 96 85 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 2860 4031 5761 2860 Size: 1171 1730 3241 Fragments arranged by size: 3241 1730 1171 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2488 3497 844 Size: 1644 1009 3489 Fragments arranged by size: 3489 1644 1009 BsrBI CCG'CTC Cuts at: 1394 1823 4029 1394 Size: 429 2206 3507 Fragments arranged by size: 3507 2206 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2950 4637 4811 65 Size: 1183 1702 1687 174 1396 Fragments arranged by size: 1702 1687 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4786 4973 1700 Size: 110 2976 187 2869 Fragments arranged by size: 2976 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6142 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6142 BstYI r'GATC_y Cuts at: 1972 2271 2398 3472 3839 4224 4241 5693 5779 Size: 299 127 1074 367 385 17 1452 86 Cuts at: 5779 5791 6137 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1074 385 367 346 299 127 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2316 2316 Size: 6142 BtgI C'CryG_G Cuts at: 513 1065 2004 2631 513 Size: 552 939 627 4024 Fragments arranged by size: 4024 939 627 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5403 Fragments arranged by size: 5403 683 56 BtsI GCAGTG_nn' Cuts at: 3658 4452 4472 3658 Size: 794 20 5328 Fragments arranged by size: 5328 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2932 2967 3100 3135 529 Size: 35 2368 35 133 35 3536 Fragments arranged by size: 3536 2368 133 35 35 35 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5149 809 Size: 507 3833 1802 Fragments arranged by size: 3833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3589 4478 6107 Size: 54 1425 50 275 18 1759 889 1629 Cuts at: 6107 8 Size: 43 Fragments arranged by size: 1759 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 1537 Size: 275 1777 4090 Fragments arranged by size: 4090 1777 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2178 2593 4072 1353 Size: 825 415 1479 3423 Fragments arranged by size: 3423 1479 825 415 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4729 5103 5249 387 Size: 1064 138 3140 374 146 1280 Fragments arranged by size: 3140 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2227 2323 2227 Size: 96 6046 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2297 2309 2312 2402 3027 3279 Size: 393 440 225 12 3 90 625 252 Cuts at: 3279 3367 4205 4802 5589 5857 1239 Size: 88 838 597 787 268 1524 Fragments arranged by size: 1524 838 787 625 597 440 393 268 252 225 90 88 12 3 EcoNI CCTnn'n_nnAGG Cuts at: 2141 2315 2478 2881 2141 Size: 174 163 403 5402 Fragments arranged by size: 5402 403 174 163 EcoO109I rG'GnC_Cy Cuts at: 2195 2195 Size: 6142 EcoRI G'AATT_C Cuts at: 2997 3081 3249 3451 3829 2997 Size: 84 168 202 378 5310 Fragments arranged by size: 5310 378 202 168 84 EcoRV GAT'ATC Cuts at: 3419 3419 Size: 6142 FspI TGC'GCA Cuts at: 4648 4648 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 5289 1678 Size: 3611 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2068 2075 2100 2107 2458 Size: 32 135 32 496 7 25 7 351 Cuts at: 2458 2490 2794 2826 4783 4815 4857 4889 1373 Size: 32 304 32 1957 32 42 32 2626 Fragments arranged by size: 2626 1957 496 351 304 135 42 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2234 3486 3732 669 Size: 988 281 296 1252 246 3079 Fragments arranged by size: 3079 1252 988 296 281 246 HindIII A'AGCT_T Cuts at: 748 2570 3210 748 Size: 1822 640 3680 Fragments arranged by size: 3680 1822 640 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KpnI G_GTAC'C Cuts at: 3467 3467 Size: 6142 MfeI C'AATT_G Cuts at: 3741 3741 Size: 6142 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3325 5256 5440 1305 Size: 2020 1931 184 2007 Fragments arranged by size: 2020 2007 1931 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4320 Fragments arranged by size: 4320 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2511 2865 2922 4100 4459 4618 518 Size: 648 1345 354 57 1178 359 159 2042 Fragments arranged by size: 2042 1345 1178 648 359 354 159 57 MspA1I CmG'CkG Cuts at: 1606 2395 3558 4235 5383 5628 1606 Size: 789 1163 677 1148 245 2120 Fragments arranged by size: 2120 1163 1148 789 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2631 513 Size: 552 939 627 4024 Fragments arranged by size: 4024 939 627 552 NdeI CA'TA_TG Cuts at: 387 3284 387 Size: 2897 3245 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6142 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 2756 3329 3500 5045 1132 Size: 97 1527 573 171 1545 2229 Fragments arranged by size: 2229 1545 1527 573 171 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6142 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 2035 2737 1108 Size: 927 702 4513 Fragments arranged by size: 4513 927 702 PfoI T'CCnGG_A Cuts at: 2220 2274 2220 Size: 54 6088 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2195 2195 Size: 6142 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PstI C_TGCA'G Cuts at: 830 3494 830 Size: 2664 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 PvuII CAG'CTG Cuts at: 2395 2395 Size: 6142 SacI G_AGCT'C Cuts at: 2229 2325 2229 Size: 96 6046 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 4390 6102 4390 Size: 1712 4430 SfcI C'TryA_G Cuts at: 826 1045 2243 3490 4625 5306 5497 826 Size: 219 1198 1247 1135 681 191 1471 Fragments arranged by size: 1471 1247 1198 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 2333 3469 1872 Size: 461 1136 4545 Fragments arranged by size: 4545 1136 461 SmlI C'TyrA_G Cuts at: 820 1017 1943 2199 4246 5147 5409 820 Size: 197 926 256 2047 901 262 1553 Fragments arranged by size: 2047 1553 926 901 262 256 197 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 3500 1132 Size: 2368 3774 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 2136 2979 3140 1517 Size: 619 843 161 4519 Fragments arranged by size: 4519 843 619 161 StyI C'CwwG_G Cuts at: 513 1065 2004 2137 2262 2631 3581 513 Size: 552 939 133 125 369 950 3074 Fragments arranged by size: 3074 950 939 552 369 133 125 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2364 3167 4169 4186 4339 4524 4969 Size: 1255 784 803 1002 17 153 185 445 Cuts at: 4969 325 Size: 1498 Fragments arranged by size: 1498 1255 1002 803 784 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2485 2759 4388 Size: 274 80 33 51 1443 508 274 1629 Cuts at: 4388 6100 96 Size: 1712 138 Fragments arranged by size: 1712 1629 1443 508 274 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2138 2170 2406 2438 6102 6134 2138 Size: 32 236 32 3664 32 2146 Fragments arranged by size: 3664 2146 236 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 2837 3478 2837 Size: 641 5501 XhoI C'TCGA_G Cuts at: 1943 2199 1943 Size: 256 5886 XmaI C'CCGG_G Cuts at: 1870 2331 3467 1870 Size: 461 1136 4545 Fragments arranged by size: 4545 1136 461 XmnI GAAnn'nnTTC Cuts at: 3296 4271 3296 Size: 975 5167 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI