(Circular) (Six-base) MAPSORT of: pFN21AB5486.seq Check: 2689 from: 1 to: 5284 pFN21AB5486 5284 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3094 278 Size: 53 83 186 936 1558 2468 Fragments arranged by size: 2468 1558 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2627 1937 Size: 690 4594 Acc65I G'GTAC_C Cuts at: 2605 2605 Size: 5284 AclI AA'CG_TT Cuts at: 1563 3411 3784 1563 Size: 1848 373 3063 Fragments arranged by size: 3063 1848 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2302 2446 3347 4731 4999 1632 Size: 670 144 901 1384 268 1917 Fragments arranged by size: 1917 1384 901 670 268 144 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5087 AflIII A'CryG_T Cuts at: 1203 4183 5160 1203 Size: 2980 977 1327 Fragments arranged by size: 2980 1327 977 AgeI A'CCGG_T Cuts at: 4115 4115 Size: 5284 AhdI GACnn_n'nnGTC Cuts at: 4013 4013 Size: 5284 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5284 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2381 1184 Size: 1197 4087 AlwNI CAG_nnn'CTG Cuts at: 2050 3027 4599 2050 Size: 977 1572 2735 Fragments arranged by size: 2735 1572 977 ApaLI G'TGCA_C Cuts at: 3341 4497 3341 Size: 1156 4128 ApoI r'AATT_y Cuts at: 1493 2507 2593 2819 2971 5080 1493 Size: 1014 86 226 152 2109 1697 Fragments arranged by size: 2109 1697 1014 226 152 86 AseI AT'TA_AT Cuts at: 160 1032 3838 160 Size: 872 2806 1606 Fragments arranged by size: 2806 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5284 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2609 1870 Size: 73 90 576 4545 Fragments arranged by size: 4545 576 90 73 BamHI G'GATC_C Cuts at: 2614 2981 2614 Size: 367 4917 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2237 2519 2605 4060 618 Size: 325 217 627 450 282 86 1455 1842 Fragments arranged by size: 1842 1455 627 450 325 282 217 86 BanII G_rGCy'C Cuts at: 1391 2603 1391 Size: 1212 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2200 928 Size: 971 301 4012 Fragments arranged by size: 4012 971 301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3475 3509 1903 Size: 34 1538 34 3678 Fragments arranged by size: 3678 1538 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3176 4386 3176 Size: 1210 4074 BclI T'GATC_A Cuts at: 1548 1548 Size: 5284 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2124 3895 136 Size: 107 122 71 631 605 452 1771 1525 Fragments arranged by size: 1771 1525 631 605 452 122 107 71 BglII A'GATC_T Cuts at: 2154 2457 5279 2154 Size: 303 2822 2159 Fragments arranged by size: 2822 2159 303 BlpI GC'TnA_GC Cuts at: 2270 2701 2270 Size: 431 4853 Bme1580I G_kGCm'C Cuts at: 1790 3345 4501 1790 Size: 1555 1156 2573 Fragments arranged by size: 2573 1555 1156 BmgBI CAC'GTC Cuts at: 1318 2206 1318 Size: 888 4396 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3015 3973 442 Size: 416 53 83 399 24 1598 958 1753 Fragments arranged by size: 1753 1598 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5284 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 2390 3944 1239 Size: 1076 75 1554 2579 Fragments arranged by size: 2579 1554 1076 75 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2093 2291 3409 4274 4572 2093 Size: 198 1118 865 298 2805 Fragments arranged by size: 2805 1118 865 298 198 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3947 882 Size: 617 2448 2219 Fragments arranged by size: 2448 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3075 493 Size: 711 1871 2702 Fragments arranged by size: 2702 1871 711 BsaBI GATnn'nnATC Cuts at: 5278 5278 Size: 5284 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3091 3473 4108 275 Size: 53 83 186 936 1558 382 635 1451 Fragments arranged by size: 1558 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3717 4115 4389 4536 4947 3717 Size: 398 274 147 411 4054 Fragments arranged by size: 4054 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2414 2444 1178 Size: 30 165 30 1011 30 4018 Fragments arranged by size: 4018 1011 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5284 BseYI C'CCAG_C Cuts at: 2564 4487 4983 2564 Size: 1923 496 2865 Fragments arranged by size: 2865 1923 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2399 1163 Size: 573 663 4048 Fragments arranged by size: 4048 663 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2734 3495 4523 1540 Size: 275 186 733 761 1028 2301 Fragments arranged by size: 2301 1028 761 733 275 186 BsiHKAI G_wGCw'C Cuts at: 2205 3345 3430 4501 2205 Size: 1140 85 1071 2988 Fragments arranged by size: 2988 1140 1071 85 BsmI GAATG_Cn' Cuts at: 2795 2888 2795 Size: 93 5191 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2205 2603 3345 3430 4501 1391 Size: 399 415 398 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 415 399 398 85 BspEI T'CCGG_A Cuts at: 4947 4947 Size: 5284 BspHI T'CATG_A Cuts at: 3173 4903 3173 Size: 1730 3554 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2058 2639 844 Size: 1214 581 3489 Fragments arranged by size: 3489 1214 581 BsrBI CCG'CTC Cuts at: 1394 1823 3171 1394 Size: 429 1348 3507 Fragments arranged by size: 3507 1348 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2262 3779 3953 65 Size: 1183 1014 1517 174 1396 Fragments arranged by size: 1517 1396 1183 1014 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2123 3928 4115 1700 Size: 110 313 1805 187 2869 Fragments arranged by size: 2869 1805 313 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5284 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5284 BssSI C'ACGA_G Cuts at: 2078 3344 4356 2078 Size: 1266 1012 3006 Fragments arranged by size: 3006 1266 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2050 794 Size: 1256 4028 BstBI TT'CG_AA Cuts at: 4088 4088 Size: 5284 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5284 BstYI r'GATC_y Cuts at: 1972 2154 2457 2614 2981 3366 3383 4835 4921 Size: 182 303 157 367 385 17 1452 86 Cuts at: 4921 4933 5279 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 303 182 157 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2136 513 Size: 552 939 132 3661 Fragments arranged by size: 3661 939 552 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4545 Fragments arranged by size: 4545 683 56 BtsI GCAGTG_nn' Cuts at: 2561 2575 2800 3594 3614 2561 Size: 14 225 794 20 4231 Fragments arranged by size: 4231 794 225 20 14 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5284 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5249 DraI TTT'AAA Cuts at: 2588 2588 Size: 5284 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4291 809 Size: 507 2975 1802 Fragments arranged by size: 2975 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2125 2731 3620 Size: 54 1425 50 275 18 295 606 889 Cuts at: 3620 5249 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 606 295 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2731 1537 Size: 275 919 4090 Fragments arranged by size: 4090 919 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3214 1353 Size: 1861 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2534 3871 4245 4391 387 Size: 1064 138 945 1337 374 146 1280 Fragments arranged by size: 1337 1280 1064 945 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2302 2315 2390 2446 3347 3944 4731 Size: 393 670 13 75 56 901 597 787 Cuts at: 4731 4999 1239 Size: 268 1524 Fragments arranged by size: 1524 901 787 670 597 393 268 75 56 13 EcoO109I rG'GnC_Cy Cuts at: 2047 2047 Size: 5284 EcoRI G'AATT_C Cuts at: 2507 2593 2971 2507 Size: 86 378 4820 Fragments arranged by size: 4820 378 86 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2332 2332 Size: 5284 FspI TGC'GCA Cuts at: 3790 3790 Size: 5284 HaeII r_GCGC'y Cuts at: 1678 4431 1678 Size: 2753 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2414 2446 3925 3957 3999 Size: 32 135 32 842 32 1479 32 42 Cuts at: 3999 4031 1373 Size: 32 2626 Fragments arranged by size: 2626 1479 842 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2628 2874 669 Size: 988 281 690 246 3079 Fragments arranged by size: 3079 988 690 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5284 HpaI GTT'AAC Cuts at: 2874 2874 Size: 5284 KpnI G_GTAC'C Cuts at: 2609 2609 Size: 5284 MfeI C'AATT_G Cuts at: 2883 2883 Size: 5284 MluI A'CGCG_T Cuts at: 5160 5160 Size: 5284 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4398 4582 1305 Size: 3093 184 2007 Fragments arranged by size: 3093 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3462 Fragments arranged by size: 3462 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3242 3601 3760 518 Size: 648 2076 359 159 2042 Fragments arranged by size: 2076 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2700 3377 4525 4770 1606 Size: 1094 677 1148 245 2120 Fragments arranged by size: 2120 1148 1094 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3793 Fragments arranged by size: 3793 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5284 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5284 NotI GC'GGCC_GC Cuts at: 2731 2731 Size: 5284 NspI r_CATG'y Cuts at: 1132 1229 2326 2642 4187 1132 Size: 97 1097 316 1545 2229 Fragments arranged by size: 2229 1545 1097 316 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5284 PciI A'CATG_T Cuts at: 4183 4183 Size: 5284 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5284 PfoI T'CCnGG_A Cuts at: 2250 2250 Size: 5284 PmeI GTTT'AAAC Cuts at: 2588 2588 Size: 5284 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3353 3385 4866 4898 3353 Size: 32 1481 32 3739 Fragments arranged by size: 3739 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 2047 Size: 5284 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5284 PsiI TTA'TAA Cuts at: 2854 2854 Size: 5284 PstI C_TGCA'G Cuts at: 830 2046 2088 2556 2636 830 Size: 1216 42 468 80 3478 Fragments arranged by size: 3478 1216 468 80 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5284 SalI G'TCGA_C Cuts at: 1936 2626 1936 Size: 690 4594 SbfI CC_TGCA'GG Cuts at: 2636 2636 Size: 5284 ScaI AGT'ACT Cuts at: 3532 5244 3532 Size: 1712 3572 SfcI C'TryA_G Cuts at: 826 1045 2042 2084 2552 2632 3767 4448 4639 Size: 219 997 42 468 80 1135 681 191 Cuts at: 4639 826 Size: 1471 Fragments arranged by size: 1471 1135 997 681 468 219 191 80 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5284 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5284 SmaI CCC'GGG Cuts at: 1872 2611 1872 Size: 739 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2108 2306 3388 4289 4551 820 Size: 197 926 165 198 1082 901 262 1553 Fragments arranged by size: 1553 1082 926 901 262 198 197 165 SnaBI TAC'GTA Cuts at: 493 3075 493 Size: 2582 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5284 SphI G_CATG'C Cuts at: 1132 2642 1132 Size: 1510 3774 SspI AAT'ATT Cuts at: 5 52 3208 5 Size: 47 3156 2081 Fragments arranged by size: 3156 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5284 StyI C'CwwG_G Cuts at: 513 1065 2004 2499 2723 513 Size: 552 939 495 224 3074 Fragments arranged by size: 3074 939 552 495 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3311 3328 3481 3666 4111 325 Size: 1255 1731 17 153 185 445 1498 Fragments arranged by size: 1731 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3530 5242 96 Size: 274 80 33 51 1443 1553 1712 138 Fragments arranged by size: 1712 1553 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5244 5276 5244 Size: 32 5252 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5284 XbaI T'CTAG_A Cuts at: 2620 2620 Size: 5284 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2392 2392 Size: 5284 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5284 XmaI C'CCGG_G Cuts at: 1870 2609 1870 Size: 739 4545 XmnI GAAnn'nnTTC Cuts at: 3413 3413 Size: 5284 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3092 276 Size: 53 83 186 936 1558 2468 Fragments arranged by size: 2468 1558 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI