Restriction map for
pFN21AB5463
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AclI AcsI AcyI
AflII AgeI AhdI Alw44I ApaI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BanI BbeI BbrPI BbvCI
BciVI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsgI BsiEI BsiWI BsmFI BsmI
BsoBI Bsp1407I BspDI BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MfeI MluI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 721 GTAGAC GT^[AC][GT]AC
AccIII 344 TCCGGA T^CCGGA
AflIII 641 ACATGT A^C[GA][CT]GT
AlwNI 568 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 336 CCTGAGG CC^T[ACGT]AGG
Aor51HI 428 AGCGCT AGC^GCT
AscI 780 GGCGCGCC GG^CGCGCC
AspHI 19 GAGCTC G[AT]GC[AT]^C
BalI 656 TGGCCA TGG^CCA
BamHI 341 GGATCC G^GATCC
BanII 19 GAGCTC G[GA]GC[CT]^C
BcgI 827 GCATCGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 800 TGATCA T^GATCA
BglI 178 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 19 GAGCTC G[AGT]GC[ACT]^C
BsaBI 801 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 77 GAGACC GGTCTC(1/5)
BsaWI 344 TCCGGA [AT]^CCGG[AT]
BseAI 344 TCCGGA T^CCGGA
BsiHKAI 19 GAGCTC G[AT]GC[AT]^C
BsmBI 586 GAGACG CGTCTC(1/5)
Bsp1286I 19 GAGCTC G[AGT]GC[ACT]^C
BspEI 344 TCCGGA T^CCGGA
BspHI 797 TCATGA T^CATGA
BspLU11I 641 ACATGT A^CATGT
BspMI 105 GCAGGT ACCTGC(4/8)
BsrBI 321 CCGCTC CCGCTC(-3/-3)
BsrFI 466 ACCGGC [GA]^CCGG[CT]
BsrI 482 CCAGT ACTGG(1/-1)
BssHII 781 GCGCGC G^CGCGC
BssSI 664 CTCGTG CACGAG(-5/-1)
BstXI 659 CCATGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 341 GGATCC [GA]^GATC[CT]
Bsu36I 336 CCTGAGG CC^T[ACGT]AGG
Cfr10I 466 ACCGGC [GA]^CCGG[CT]
DsaI 68 CCGTGG C^C[GA][CT]GG
Eco47III 428 AGCGCT AGC^GCT
Eco81I 336 CCTGAGG CC^T[ACGT]AGG
EcoT14I 32 CCAAGG C^C[AT][AT]GG
FauI 520 CCCGC CCCGC(4/6)
FbaI 800 TGATCA T^GATCA
HaeII 428 AGCGCT [GA]GCGC^[CT]
HgaI 588 GACGC GACGC(5/10)
MaeII 149 ACGT A^CGT
MaeIII 64 GTCAC ^GT[ACGT]AC
MamI 801 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 341 GGATCC [GA]^GATC[CT]
MluNI 656 TGGCCA TGG^CCA
MroI 344 TCCGGA T^CCGGA
MscI 656 TGGCCA TGG^CCA
MseI 277 TTAA T^TAA
PleI 550 GACTC GAGTC(4/5)
PvuII 454 CAGCTG CAG^CTG
RcaI 797 TCATGA T^CATGA
SacI 19 GAGCTC GAGCT^C
StuI 312 AGGCCT AGG^CCT
StyI 32 CCAAGG C^C[AT][AT]GG
TfiI 117 GATTC G^A[AT]TC
Tru9I 277 TTAA T^TAA
Tsp45I 64 GTCAC ^GT[GC]AC
XhoII 341 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 10 enzymes) #
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BbvI 453 GCAGC GCAGC(8/12)
431 GCTGC
BsmAI 77 GAGAC GTCTC(1/5)
586 GAGAC
DdeI 337 CTGAG C^T[ACGT]AG
574 CTGAG
EaeI 29 CGGCCA [CT]^GGCC[GA]
656 TGGCCA
HinfI 117 GATTC G^A[ACGT]TC
550 GACTC
MspA1I 329 CCGCTG C[AC]G^C[GT]G
454 CAGCTG
NlaIV 341 GGATCC GG[ACGT]^[ACGT]CC
515 GGAACC
NspI 500 ACATGC [GA]CATG^[CT]
641 ACATGT
TseI 431 GCTGC G^C[AT]GC
453 GCAGC
XcmI 371 CCAGAAAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
482 CCAGTGAGGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbsI BpmI BpuAI BsaJI
BseRI BsiYI BslI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MboII MnlI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TspRI TthHB8I