Restriction map for
pFN21AB5457
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI AocI
Aor51HI AscI AseI AsnI AspEI
AspI AvaI AviII BamHI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglII BpuAI BsaAI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MfeI MflI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI TfiI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 264 GGTACC G^GTACC
Alw44I 556 GTGCAC G^TGCAC
AlwI 209 GGATC GGATC(4/5)
AlwNI 166 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 401 GGGCCC GGGCC^C
ApaLI 556 GTGCAC G^TGCAC
Asp700I 948 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 264 GGTACC G^GTACC
AspHI 556 GTGCAC G[AT]GC[AT]^C
AvrII 1116 CCTAGG C^CTAGG
BciVI 276 GGATAC GTATCC(6/5)
BlnI 1116 CCTAGG C^CTAGG
BlpI 1333 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1333 GCTCAGC GC^T[ACGT]AGC
BsaBI 696 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 556 GTGCAC G[AT]GC[AT]^C
BsmI 1250 GCATTC GAATGC(1/-1)
BspMI 746 ACCTGC ACCTGC(4/8)
BsrBI 375 GAGCGG CCGCTC(-3/-3)
BsrDI 477 CATTGC GCAATG(2/0)
CelII 1333 GCTCAGC GC^T[ACGT]AGC
DraIII 541 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 890 GAATTC G^AATTC
EcoT22I 1133 ATGCAT ATGCA^T
KpnI 264 GGTACC GGTAC^C
MaeII 620 ACGT A^CGT
MamI 696 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1413 CCATGG C^CATGG
NheI 898 GCTAGC G^CTAGC
NsiI 1133 ATGCAT ATGCA^T
NspI 581 ACATGC [GA]CATG^[CT]
PpuMI 915 GGGTCCC [GA]G^G[AT]CC[CT]
SspI 712 AATATT AAT^ATT
StuI 653 AGGCCT AGG^CCT
XmnI 948 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccII 18 CGCG CG^CG
771 CGCG
BalI 128 TGGCCA TGG^CCA
1373 TGGCCA
BanII 401 GGGCCC G[GA]GC[CT]^C
1352 GGGCTC
BglI 130 GCCAATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1412 GCCATGGTGGC
BpmI 497 CTGGAG CTGGAG(16/14)
958 CTGGAG
BsaI 341 GAGACC GGTCTC(1/5)
183 GGTCTC
BstUI 18 CGCG CG^CG
771 CGCG
BstXI 149 CCAAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
930 CCAGTTTGATGG
DdeI 1024 CTAAG C^T[ACGT]AG
1334 CTCAG
DraII 400 GGGGCCC [GA]G^G[ACGT]CC[CT]
915 GGGTCCC
DsaI 72 CCACGG C^C[GA][CT]GG
1413 CCATGG
EcoNI 219 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1323 CCTCATCCAGG
EcoO109I 400 GGGGCCC [GA]G^G[ACGT]CC[CT]
915 GGGTCCC
MluNI 128 TGGCCA TGG^CCA
1373 TGGCCA
MscI 128 TGGCCA TGG^CCA
1373 TGGCCA
MslI 1251 CATTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1357 CACACCTATG
MvnI 18 CGCG CG^CG
771 CGCG
PvuII 586 CAGCTG CAG^CTG
1190 CAGCTG
TaqI 254 TCGA T^CGA
1143 TCGA
TthHB8I 254 TCGA T^CGA
1143 TCGA
XcmI 726 CCAAGACACCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
803 CCATCTCCGCCCTGG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BanI BbvI BcnI BfaI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DpnII
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI