Restriction map for
pFN21AB5457
(Linear) (Six-base) MAPSORT of: KIBB5457.seq Check: 1602 from: 1 to: 1449
KIBB5457.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 755 1449
Size: 755 694
Acc65I G'GTAC_C
Cuts at: 0 264 1449
Size: 264 1185
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 898 931 1141 1449
Size: 214 684 33 210 308
Fragments arranged by size:
684 308 214 210 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 763 1273 1449
Size: 763 510 176
Fragments arranged by size:
763 510 176
AlwNI CAG_nnn'CTG
Cuts at: 0 171 1449
Size: 171 1278
ApaI G_GGCC'C
Cuts at: 0 405 1449
Size: 405 1044
ApaLI G'TGCA_C
Cuts at: 0 556 1449
Size: 556 893
ApoI r'AATT_y
Cuts at: 0 306 359 890 1449
Size: 306 53 531 559
Fragments arranged by size:
559 531 306 53
AvrII C'CTAG_G
Cuts at: 0 1116 1449
Size: 1116 333
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 256 289 1449
Size: 256 33 1160
Fragments arranged by size:
1160 256 33
BanI G'GyrC_C
Cuts at: 0 160 264 335 1420 1449
Size: 160 104 71 1085 29
Fragments arranged by size:
1085 160 104 71 29
BanII G_rGCy'C
Cuts at: 0 405 1356 1449
Size: 405 951 93
Fragments arranged by size:
951 405 93
BciVI GTATCCnnnnn_n'
Cuts at: 0 270 1449
Size: 270 1179
BfrBI ATG'CAT
Cuts at: 0 1135 1449
Size: 1135 314
BglI GCCn_nnn'nGGC
Cuts at: 0 136 1418 1449
Size: 136 1282 31
Fragments arranged by size:
1282 136 31
BlpI GC'TnA_GC
Cuts at: 0 1334 1449
Size: 1334 115
Bme1580I G_kGCm'C
Cuts at: 0 165 405 560 787 1449
Size: 165 240 155 227 662
Fragments arranged by size:
662 240 227 165 155
BmgBI CAC'GTC
Cuts at: 0 621 1449
Size: 621 828
BmrI ACTGGGnnnn_n'
Cuts at: 0 159 525 1244 1449
Size: 159 366 719 205
Fragments arranged by size:
719 366 205 159
BmtI G_CTAG'C
Cuts at: 0 902 1449
Size: 902 547
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 201 702 1449
Size: 201 501 747
Fragments arranged by size:
747 501 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 979 1449
Size: 518 461 470
Fragments arranged by size:
518 470 461
Bpu10I CC'TnA_GC
Cuts at: 0 1024 1449
Size: 1024 425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 703 992 1449
Size: 331 372 289 457
Fragments arranged by size:
457 372 331 289
BsaI GGTCTCn'nnnn_
Cuts at: 0 189 335 1449
Size: 189 146 1114
Fragments arranged by size:
1114 189 146
BsaBI GATnn'nnATC
Cuts at: 0 700 1449
Size: 700 749
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 836 866 1449
Size: 836 30 583
Fragments arranged by size:
836 583 30
BseYI C'CCAG_C
Cuts at: 0 229 624 1449
Size: 229 395 825
Fragments arranged by size:
825 395 229
BsiHKAI G_wGCw'C
Cuts at: 0 560 1449
Size: 560 889
BsmI GAATG_Cn'
Cuts at: 0 1250 1449
Size: 1250 199
Bsp1286I G_dGCh'C
Cuts at: 0 165 405 560 787 1356 1449
Size: 165 240 155 227 569 93
Fragments arranged by size:
569 240 227 165 155 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 755 1449
Size: 755 694
BsrBI CCG'CTC
Cuts at: 0 377 1449
Size: 377 1072
BsrDI GCAATG_nn'
Cuts at: 0 476 1449
Size: 476 973
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 937 1449
Size: 156 781 512
Fragments arranged by size:
781 512 156
BtgI C'CryG_G
Cuts at: 0 72 1413 1449
Size: 72 1341 36
Fragments arranged by size:
1341 72 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 401 1056 1449
Size: 401 655 393
Fragments arranged by size:
655 401 393
BtsI GCAGTG_nn'
Cuts at: 0 1271 1449
Size: 1271 178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1026 1047 1061 1082 1449
Size: 1026 21 14 21 367
Fragments arranged by size:
1026 367 21 21 14
DraIII CAC_nnn'GTG
Cuts at: 0 546 1449
Size: 546 903
EaeI y'GGCC_r
Cuts at: 0 32 128 1373 1449
Size: 32 96 1245 76
Fragments arranged by size:
1245 96 76 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 222 798 1449
Size: 222 576 651
Fragments arranged by size:
651 576 222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 518 898 931 979 1141 1449
Size: 214 304 380 33 48 162 308
Fragments arranged by size:
380 308 304 214 162 48 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 223 1327 1449
Size: 223 1104 122
Fragments arranged by size:
1104 223 122
EcoO109I rG'GnC_Cy
Cuts at: 0 401 916 1449
Size: 401 515 533
Fragments arranged by size:
533 515 401
EcoRI G'AATT_C
Cuts at: 0 890 1449
Size: 890 559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 483 1449
Size: 483 966
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 836 868 934 966 1449
Size: 20 32 784 32 66 32 483
Fragments arranged by size:
784 483 66 32 32 32 20
KpnI G_GTAC'C
Cuts at: 0 268 1449
Size: 268 1181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1449
Size: 254 1195
MscI TGG'CCA
Cuts at: 0 130 1375 1449
Size: 130 1245 74
Fragments arranged by size:
1245 130 74
MslI CAynn'nnrTG
Cuts at: 0 1255 1361 1449
Size: 1255 106 88
Fragments arranged by size:
1255 106 88
MspA1I CmG'CkG
Cuts at: 0 588 1192 1338 1449
Size: 588 604 146 111
Fragments arranged by size:
604 588 146 111
NcoI C'CATG_G
Cuts at: 0 1413 1449
Size: 1413 36
NheI G'CTAG_C
Cuts at: 0 898 1449
Size: 898 551
NsiI A_TGCA'T
Cuts at: 0 1137 1449
Size: 1137 312
NspI r_CATG'y
Cuts at: 0 585 1449
Size: 585 864
PfoI T'CCnGG_A
Cuts at: 0 956 1449
Size: 956 493
PpuMI rG'GwC_Cy
Cuts at: 0 916 1449
Size: 916 533
PspOMI G'GGCC_C
Cuts at: 0 401 1449
Size: 401 1048
PvuII CAG'CTG
Cuts at: 0 588 1192 1449
Size: 588 604 257
Fragments arranged by size:
604 588 257
SanDI GG'GwC_CC
Cuts at: 0 916 1449
Size: 916 533
SmlI C'TyrA_G
Cuts at: 0 346 718 1007 1449
Size: 346 372 289 442
Fragments arranged by size:
442 372 346 289
SspI AAT'ATT
Cuts at: 0 714 1449
Size: 714 735
StuI AGG'CCT
Cuts at: 0 655 1449
Size: 655 794
StyI C'CwwG_G
Cuts at: 0 353 471 1116 1413 1449
Size: 353 118 645 297 36
Fragments arranged by size:
645 353 297 118 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 507 1449
Size: 148 359 942
Fragments arranged by size:
942 359 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 137 169 1449
Size: 137 32 1280
Fragments arranged by size:
1280 137 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 733 810 1449
Size: 733 77 639
Fragments arranged by size:
733 639 77
XmnI GAAnn'nnTTC
Cuts at: 0 952 1449
Size: 952 497
Enzymes that do cut:
AarI Acc65I AcuI AlfI AlwNI ApaI ApaLI ApoI
AvrII BaeI BanI BanII BciVI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaXI BseYI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrDI BstXI BtgI BtgZI BtsI CspCI DraIII
EaeI EciI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
KpnI MmeI MscI MslI MspA1I NcoI NheI NsiI
NspI PfoI PpuMI PspOMI PvuII SanDI SmlI SspI
StuI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AscI AseI AsiSI AvaI BamHI BbeI
BbsI BbvCI BcgI BclI BglII BsaAI BsaHI BsaWI
BseRI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I ClaI DraI DrdI EagI EarI EcoICRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI NaeI NarI NdeI NgoMIV NotI NruI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TatI Tth111I XbaI XhoI XmaI ZraI