Restriction map for
pFN21AB5400
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AvaI
AviII AvrII BamHI BbrPI BbvCI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KspI MamI MflI MluI
MroI MvnI NaeI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1225 GGTACC G^GTACC
AfaI 1226 GTAC GT^AC
AocI 560 CCTCAGG CC^T[ACGT]AGG
Asp718I 1225 GGTACC G^GTACC
BalI 513 TGGCCA TGG^CCA
BcgI 1137 GCAGGAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 361 CTAG C^TAG
BsmAI 936 GAGAC GTCTC(1/5)
BsmFI 702 GGGAC GGGAC(10/14)
BsmI 1357 GCATTC GAATGC(1/-1)
BssSI 875 CTCGTG CACGAG(-5/-1)
BstBI 455 TTCGAA TT^CGAA
Bsu36I 560 CCTCAGG CC^T[ACGT]AGG
DpnII 547 GATC ^GATC
DraIII 398 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 513 TGGCCA [CT]^GGCC[GA]
Eco81I 560 CCTCAGG CC^T[ACGT]AGG
EcoT14I 677 CCATGG C^C[AT][AT]GG
HapII 1261 CCGG C^CGG
HpaII 1261 CCGG C^CGG
KpnI 1225 GGTACC GGTAC^C
MaeI 361 CTAG C^TAG
MaeII 1401 ACGT A^CGT
MaeIII 664 GTGAC ^GT[ACGT]AC
MboI 547 GATC ^GATC
MfeI 1337 CAATTG C^AATTG
MluNI 513 TGGCCA TGG^CCA
MscI 513 TGGCCA TGG^CCA
MseI 20 TTAA T^TAA
MspI 1261 CCGG C^CGG
MunI 1337 CAATTG C^AATTG
NcoI 677 CCATGG C^CATGG
NdeII 547 GATC ^GATC
NspV 455 TTCGAA TT^CGAA
PpuMI 1242 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 1226 GTAC GT^AC
SacI 1021 GAGCTC GAGCT^C
Sau3AI 547 GATC ^GATC
SexAI 920 ACCAGGT A^CC[AT]GGT
SfcI 530 CTGTAG C^T[GA][CT]AG
SfuI 455 TTCGAA TT^CGAA
StyI 677 CCATGG C^C[AT][AT]GG
Tru9I 20 TTAA T^TAA
Tsp45I 664 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 180 GAATTT [GA]^AATT[CT]
1384 GAATTT
AcyI 953 GGCGCC G[GA]^CG[CT]C
1046 GGCGCC
AlwNI 1241 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1370 CAGCTCCTG
ApoI 180 GAATTT [GA]^AATT[CT]
1384 GAATTT
Asp700I 201 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
961 GAAGAACTTC
AspHI 916 GAGCAC G[AT]GC[AT]^C
1021 GAGCTC
BanII 673 GAGCCC G[GA]GC[CT]^C
1021 GAGCTC
BbeI 953 GGCGCC GGCGC^C
1046 GGCGCC
BbsI 201 GAAGAC GAAGAC(2/6)
307 GTCTTC
BpuAI 201 GAAGAC GAAGAC(2/6)
307 GTCTTC
BsaHI 953 GGCGCC G[GA]^CG[CT]C
1046 GGCGCC
BsaJI 402 CCGTGG C^C[ACGT][ACGT]GG
677 CCATGG
BseRI 1235 CTCCTC GAGGAG(10/8)
1349 CTCCTC
BsiHKAI 916 GAGCAC G[AT]GC[AT]^C
1021 GAGCTC
BspMI 123 ACCTGC ACCTGC(4/8)
1245 ACCTGC
BsrI 1017 ACTGG ACTGG(1/-1)
684 CCAGT
DraII 556 AGGCCCT [GA]G^G[ACGT]CC[CT]
1242 AGGACCT
DsaI 402 CCGTGG C^C[GA][CT]GG
677 CCATGG
EcoO109I 556 AGGCCCT [GA]G^G[ACGT]CC[CT]
1242 AGGACCT
HaeII 953 GGCGCC [GA]GCGC^[CT]
1046 GGCGCC
Hin1I 953 GGCGCC G[GA]^CG[CT]C
1046 GGCGCC
HinfI 1106 GATTC G^A[ACGT]TC
1143 GATTC
HphI 66 TCACC GGTGA(8/7)
209 TCACC
KasI 953 GGCGCC G^GCGCC
1046 GGCGCC
NarI 953 GGCGCC GG^CGCC
1046 GGCGCC
PflMI 397 CCACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
803 CCATGCGCTGG
SapI 979 GAAGAGC GCTCTTC(1/4)
1036 GAAGAGC
SfoI 953 GGCGCC GGC^GCC
1046 GGCGCC
TaqI 456 TCGA T^CGA
549 TCGA
TfiI 1106 GATTC G^A[AT]TC
1143 GATTC
TspRI 255 CACTG CA[GC]TG[ACGT][ACGT]^
624 CAGTG
TthHB8I 456 TCGA T^CGA
549 TCGA
Van91I 397 CCACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
803 CCATGCGCTGG
XcmI 374 CCAAAGTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
479 CCAACGAACTGCTGG
XmnI 201 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
961 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AvaII BanI BbvI
BmyI BpmI BsgI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI EarI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
ItaI Ksp632I MboII MnlI MslI
MspA1I MvaI MwoI NlaIII NlaIV
PvuII Sau96I ScrFI SfaNI TseI
Tsp509I