(Circular) (Six-base) MAPSORT of: pFN21AB5377.seq Check: 9365 from: 1 to: 5725 pFN21AB5377 5725 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3068 1937 Size: 1131 4594 Acc65I G'GTAC_C Cuts at: 2264 3046 2264 Size: 782 4943 AclI AA'CG_TT Cuts at: 1563 3852 4225 1563 Size: 2289 373 3063 Fragments arranged by size: 3063 2289 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3788 5172 5440 1632 Size: 2156 1384 268 1917 Fragments arranged by size: 2156 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 4624 5601 1203 Size: 3421 977 1327 Fragments arranged by size: 3421 1327 977 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 4454 4454 Size: 5725 AleI CACnn'nnGTG Cuts at: 1166 2432 1166 Size: 1266 4459 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5725 AlwNI CAG_nnn'CTG Cuts at: 2089 2538 3468 5040 2089 Size: 449 930 1572 2774 Fragments arranged by size: 2774 1572 930 449 ApaLI G'TGCA_C Cuts at: 3782 4938 3782 Size: 1156 4569 ApoI r'AATT_y Cuts at: 1493 3034 3260 3412 5521 1493 Size: 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 2927 3050 1870 Size: 73 984 123 4545 Fragments arranged by size: 4545 984 123 73 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 2255 2264 3046 4501 Size: 325 217 627 336 132 9 782 1455 Cuts at: 4501 618 Size: 1842 Fragments arranged by size: 1842 1455 782 627 336 325 217 132 9 BanII G_rGCy'C Cuts at: 1391 2121 2469 3044 1391 Size: 730 348 575 4072 Fragments arranged by size: 4072 730 575 348 BbeI G_GCGC'C Cuts at: 2127 2127 Size: 5725 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2610 928 Size: 971 711 4043 Fragments arranged by size: 4043 971 711 BbvCI CC'TCA_GC Cuts at: 2151 2151 Size: 5725 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3916 3950 1903 Size: 34 1979 34 3678 Fragments arranged by size: 3678 1979 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3617 4827 3617 Size: 1210 4515 BclI T'GATC_A Cuts at: 1548 1548 Size: 5725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2238 4336 136 Size: 107 122 71 631 605 566 2098 1525 Fragments arranged by size: 2098 1525 631 605 566 122 107 71 BglII A'GATC_T Cuts at: 2190 2526 5720 2190 Size: 336 3194 2195 Fragments arranged by size: 3194 2195 336 BlpI GC'TnA_GC Cuts at: 2168 3142 2168 Size: 974 4751 Bme1580I G_kGCm'C Cuts at: 1790 3786 4942 1790 Size: 1996 1156 2573 Fragments arranged by size: 2573 1996 1156 BmgBI CAC'GTC Cuts at: 1318 2164 1318 Size: 846 4879 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2229 2486 3456 Size: 416 53 83 399 24 812 257 970 Cuts at: 3456 4414 442 Size: 958 1753 Fragments arranged by size: 1753 970 958 812 416 399 257 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2924 2924 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2919 4385 1239 Size: 1680 1466 2579 Fragments arranged by size: 2579 1680 1466 Bpu10I CC'TnA_GC Cuts at: 2151 2151 Size: 5725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3026 3850 4715 5013 3026 Size: 824 865 298 3738 Fragments arranged by size: 3738 865 824 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2286 2670 4388 882 Size: 617 787 384 1718 2219 Fragments arranged by size: 2219 1718 787 617 384 BsaAI yAC'GTr Cuts at: 493 1204 3516 493 Size: 711 2312 2702 Fragments arranged by size: 2702 2312 711 BsaBI GATnn'nnATC Cuts at: 2555 5719 2555 Size: 3164 2561 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 3532 3914 4549 Size: 53 83 186 936 591 1408 382 635 Cuts at: 4549 275 Size: 1451 Fragments arranged by size: 1451 1408 936 635 591 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4158 4556 4830 4977 5388 4158 Size: 398 274 147 411 4495 Fragments arranged by size: 4495 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2669 2699 2786 2816 1178 Size: 30 165 30 1266 30 87 30 4087 Fragments arranged by size: 4087 1266 165 87 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2144 1186 Size: 886 72 4767 Fragments arranged by size: 4767 886 72 BseYI C'CCAG_C Cuts at: 4928 5424 4928 Size: 496 5229 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2794 1163 Size: 573 1058 4094 Fragments arranged by size: 4094 1058 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2200 3175 3936 4964 1540 Size: 275 186 199 975 761 1028 2301 Fragments arranged by size: 2301 1028 975 761 275 199 186 BsiHKAI G_wGCw'C Cuts at: 2169 3786 3871 4942 2169 Size: 1617 85 1071 2952 Fragments arranged by size: 2952 1617 1071 85 BsmI GAATG_Cn' Cuts at: 2592 3236 3329 2592 Size: 644 93 4988 Fragments arranged by size: 4988 644 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2169 2469 3044 3786 3871 4942 Size: 399 331 48 300 575 742 85 1071 Cuts at: 4942 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 575 399 331 300 85 48 BspEI T'CCGG_A Cuts at: 5388 5388 Size: 5725 BspHI T'CATG_A Cuts at: 3614 5344 3614 Size: 1730 3995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3001 3080 844 Size: 2157 79 3489 Fragments arranged by size: 3489 2157 79 BsrBI CCG'CTC Cuts at: 1394 1823 2448 2454 3612 1394 Size: 429 625 6 1158 3507 Fragments arranged by size: 3507 1158 625 429 6 BsrDI GCAATG_nn' Cuts at: 65 1248 4220 4394 65 Size: 1183 2972 174 1396 Fragments arranged by size: 2972 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4369 4556 1700 Size: 110 2559 187 2869 Fragments arranged by size: 2869 2559 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5725 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 3785 4797 3785 Size: 1012 4713 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5725 BstBI TT'CG_AA Cuts at: 2596 4529 2596 Size: 1933 3792 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5725 BstYI r'GATC_y Cuts at: 1972 2190 2485 2526 3055 3422 3807 3824 5276 Size: 218 295 41 529 367 385 17 1452 Cuts at: 5276 5362 5374 5720 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 529 385 367 346 295 218 86 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2041 2287 513 Size: 552 939 37 246 3951 Fragments arranged by size: 3951 939 552 246 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4986 Fragments arranged by size: 4986 683 56 BtsI GCAGTG_nn' Cuts at: 3241 4035 4055 3241 Size: 794 20 4911 Fragments arranged by size: 4911 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5690 DraI TTT'AAA Cuts at: 2721 3029 2721 Size: 308 5417 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2510 4732 809 Size: 507 1194 2222 1802 Fragments arranged by size: 2222 1802 1194 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2443 3172 4061 Size: 54 1425 50 275 18 613 729 889 Cuts at: 4061 5690 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 729 613 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3172 1537 Size: 275 1360 4090 Fragments arranged by size: 4090 1360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2517 3655 1353 Size: 1164 1138 3423 Fragments arranged by size: 3423 1164 1138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4312 4686 4832 387 Size: 1064 138 2723 374 146 1280 Fragments arranged by size: 2723 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2919 3788 4385 5172 5440 1239 Size: 393 1287 869 597 787 268 1524 Fragments arranged by size: 1524 1287 869 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2792 2901 2792 Size: 109 5616 EcoO109I rG'GnC_Cy Cuts at: 2941 2941 Size: 5725 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 EcoRV GAT'ATC Cuts at: 2707 2707 Size: 5725 FspI TGC'GCA Cuts at: 4231 4231 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 2090 2127 4872 1678 Size: 412 37 2745 2531 Fragments arranged by size: 2745 2531 412 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 2669 2701 4366 Size: 32 135 32 809 32 256 32 1665 Cuts at: 4366 4398 4440 4472 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1665 809 256 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3069 3315 669 Size: 988 281 1131 246 3079 Fragments arranged by size: 3079 1131 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5725 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KasI G'GCGC_C Cuts at: 2123 2123 Size: 5725 KpnI G_GTAC'C Cuts at: 2268 3050 2268 Size: 782 4943 MfeI C'AATT_G Cuts at: 3324 3324 Size: 5725 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2031 2712 4839 5023 1305 Size: 726 681 2127 184 2007 Fragments arranged by size: 2127 2007 726 681 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3903 Fragments arranged by size: 3903 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2432 2624 3683 4042 4201 518 Size: 648 1266 192 1059 359 159 2042 Fragments arranged by size: 2042 1266 1059 648 359 192 159 MspA1I CmG'CkG Cuts at: 1606 2043 2359 2535 2702 3141 3818 4966 5211 Size: 437 316 176 167 439 677 1148 245 Cuts at: 5211 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 439 437 316 245 176 167 NarI GG'CG_CC Cuts at: 2124 2124 Size: 5725 NcoI C'CATG_G Cuts at: 513 1065 2004 2287 513 Size: 552 939 283 3951 Fragments arranged by size: 3951 939 552 283 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NspI r_CATG'y Cuts at: 1132 1229 2884 3083 4628 1132 Size: 97 1655 199 1545 2229 Fragments arranged by size: 2229 1655 1545 199 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 4624 4624 Size: 5725 PflMI CCAn_nnn'nTGG Cuts at: 1108 2029 2399 1108 Size: 921 370 4434 Fragments arranged by size: 4434 921 370 PfoI T'CCnGG_A Cuts at: 2790 2896 2790 Size: 106 5619 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2120 2152 3794 3826 5307 5339 2120 Size: 32 1642 32 1481 32 2506 Fragments arranged by size: 2506 1642 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2941 2941 Size: 5725 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PstI C_TGCA'G Cuts at: 830 3077 830 Size: 2247 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 PvuII CAG'CTG Cuts at: 2702 2702 Size: 5725 SacII CC_GC'GG Cuts at: 2044 2044 Size: 5725 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SbfI CC_TGCA'GG Cuts at: 3077 3077 Size: 5725 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SfcI C'TryA_G Cuts at: 826 1045 2530 3073 4208 4889 5080 826 Size: 219 1485 543 1135 681 191 1471 Fragments arranged by size: 1485 1471 1135 681 543 219 191 SfoI GGC'GCC Cuts at: 2125 2125 Size: 5725 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 3052 1872 Size: 1180 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3005 3829 4730 4992 820 Size: 197 926 1062 824 901 262 1553 Fragments arranged by size: 1553 1062 926 901 824 262 197 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 2884 3083 1132 Size: 1752 199 3774 Fragments arranged by size: 3774 1752 199 SspI AAT'ATT Cuts at: 5 52 2613 3649 5 Size: 47 2561 1036 2081 Fragments arranged by size: 2561 2081 1036 47 StuI AGG'CCT Cuts at: 1517 2156 2217 2875 2907 1517 Size: 639 61 658 32 4335 Fragments arranged by size: 4335 658 639 61 32 StyI C'CwwG_G Cuts at: 513 1065 2004 2287 2667 3164 513 Size: 552 939 283 380 497 3074 Fragments arranged by size: 3074 939 552 497 380 283 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2408 2513 3752 3769 3922 4107 4552 Size: 1255 828 105 1239 17 153 185 445 Cuts at: 4552 325 Size: 1498 Fragments arranged by size: 1498 1255 1239 828 445 185 153 105 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3971 5683 96 Size: 274 80 33 51 1443 1994 1712 138 Fragments arranged by size: 1994 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2786 2818 5685 5717 2786 Size: 32 2867 32 2794 Fragments arranged by size: 2867 2794 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5725 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 3050 1870 Size: 1180 4545 XmnI GAAnn'nnTTC Cuts at: 2979 3854 2979 Size: 875 4850 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI