Restriction map for
pFN21AB7894
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI Asp718I AspI AvrII
BbeI BbsI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MseI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 1536 GACGTC GACGT^C
AccIII 164 TCCGGA T^CCGGA
AflIII 1485 ACGTGT A^C[GA][CT]GT
AocI 808 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1508 AGCGCT AGC^GCT
AviII 1865 TGCGCA TGC^GCA
BamHI 858 GGATCC G^GATCC
BbrPI 1484 CACGTG CAC^GTG
BfaI 238 CTAG C^TAG
BglI 702 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1448 AGATCT A^GATCT
BseAI 164 TCCGGA T^CCGGA
BsiEI 1962 CGACCG CG[GA][CT]^CG
BspEI 164 TCCGGA T^CCGGA
BspHI 116 TCATGA T^CATGA
BstAPI 1389 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 808 CCTGAGG CC^T[ACGT]AGG
Eco47III 1508 AGCGCT AGC^GCT
Eco81I 808 CCTGAGG CC^T[ACGT]AGG
EcoNI 867 CCTGTATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1865 TGCGCA TGC^GCA
MaeI 238 CTAG C^TAG
MroI 164 TCCGGA T^CCGGA
MslI 1869 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 1119 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1484 CACGTG CAC^GTG
PmlI 1484 CACGTG CAC^GTG
PshAI 538 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 116 TCATGA T^CATGA
SacI 1720 GAGCTC GAGCT^C
SapI 1267 GAAGAGC GCTCTTC(1/4)
ScaI 650 AGTACT AGT^ACT
SfiI 701 GGCCCCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1360 CTTGAG C^T[CT][GA]AG
StuI 331 AGGCCT AGG^CCT
TaqI 1289 TCGA T^CGA
TthHB8I 1289 TCGA T^CGA
Van91I 1119 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1533 CCAGACGTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AhdI 46 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1102 GACCCTGCGTC
AlwNI 1120 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1636 CAGGTGCTG
Asp700I 570 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1291 GAACGCATTC
AspEI 46 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1102 GACCCTGCGTC
BalI 182 TGGCCA TGG^CCA
443 TGGCCA
BanI 1469 GGCACC G^G[CT][GA]CC
1694 GGTGCC
BsaWI 99 ACCGGA [AT]^CCGG[AT]
164 TCCGGA
BseRI 1024 GAGGAG GAGGAG(10/8)
1408 GAGGAG
BsmI 69 GAATGC GAATGC(1/-1)
1295 GCATTC
BsrBI 271 GAGCGG CCGCTC(-3/-3)
286 GAGCGG
BsrFI 608 GCCGGC [GA]^CCGG[CT]
707 GCCGGC
Cfr10I 608 GCCGGC [GA]^CCGG[CT]
707 GCCGGC
DsaI 150 CCATGG C^C[GA][CT]GG
903 CCATGG
Eam1105I 46 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1102 GACCCTGCGTC
MluNI 182 TGGCCA TGG^CCA
443 TGGCCA
MscI 182 TGGCCA TGG^CCA
443 TGGCCA
MspA1I 625 CAGCTG C[AC]G^C[GT]G
1676 CAGCTG
NaeI 608 GCCGGC GCC^GGC
707 GCCGGC
NcoI 150 CCATGG C^CATGG
903 CCATGG
NgoMIV 608 GCCGGC G^CCGGC
707 GCCGGC
PstI 352 CTGCAG CTGCA^G
1444 CTGCAG
PvuII 625 CAGCTG CAG^CTG
1676 CAGCTG
SmaI 305 CCCGGG CCC^GGG
635 CCCGGG
XmaCI 305 CCCGGG C^CCGGG
635 CCCGGG
XmaI 305 CCCGGG C^CCGGG
635 CCCGGG
XmnI 570 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1291 GAACGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaAI BsaHI BsaJI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp45I TspRI XhoII