(Circular) (Six-base) MAPSORT of: pFN21AB7894.seq Check: 4813 from: 1 to: 6673 pFN21AB7894 6673 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3545 4483 278 Size: 53 83 186 936 2009 938 2468 Fragments arranged by size: 2468 2009 938 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4016 1937 Size: 2079 4594 Acc65I G'GTAC_C Cuts at: 3994 3994 Size: 6673 AclI AA'CG_TT Cuts at: 1563 4800 5173 1563 Size: 3237 373 3063 Fragments arranged by size: 3237 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3291 3320 4736 6120 6388 1632 Size: 1659 29 1416 1384 268 1917 Fragments arranged by size: 1917 1659 1416 1384 268 29 AfeI AGC'GCT Cuts at: 3515 3515 Size: 6673 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6476 AflIII A'CryG_T Cuts at: 1203 3490 5572 6549 1203 Size: 2287 2082 977 1327 Fragments arranged by size: 2287 2082 1327 977 AgeI A'CCGG_T Cuts at: 5504 5504 Size: 6673 AhdI GACnn_n'nnGTC Cuts at: 2056 3112 5402 2056 Size: 1056 2290 3327 Fragments arranged by size: 3327 2290 1056 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6673 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3305 3336 1184 Size: 2121 31 4521 Fragments arranged by size: 4521 2121 31 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3339 3371 3339 Size: 32 6641 AlwNI CAG_nnn'CTG Cuts at: 3130 3646 4416 5988 3130 Size: 516 770 1572 3815 Fragments arranged by size: 3815 1572 770 516 ApaI G_GGCC'C Cuts at: 2591 2641 3138 2591 Size: 50 497 6126 Fragments arranged by size: 6126 497 50 ApaLI G'TGCA_C Cuts at: 4730 5886 4730 Size: 1156 5517 ApoI r'AATT_y Cuts at: 1493 3982 4208 4360 6469 1493 Size: 2489 226 152 2109 1697 Fragments arranged by size: 2489 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5227 160 Size: 872 4195 1606 Fragments arranged by size: 4195 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6673 AvaI C'yCGr_G Cuts at: 1870 1943 2310 2403 2640 3653 3998 1870 Size: 73 367 93 237 1013 345 4545 Fragments arranged by size: 4545 1013 367 345 237 93 73 BamHI G'GATC_C Cuts at: 2863 4003 4370 2863 Size: 1140 367 5166 Fragments arranged by size: 5166 1140 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3474 3699 3994 5449 618 Size: 325 217 627 1687 225 295 1455 1842 Fragments arranged by size: 1842 1687 1455 627 325 295 225 217 BanII G_rGCy'C Cuts at: 1391 2310 2411 2591 2641 3138 3260 3729 3992 Size: 919 101 180 50 497 122 469 263 Cuts at: 3992 1391 Size: 4072 Fragments arranged by size: 4072 919 497 469 263 180 122 101 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5702 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4864 4898 1903 Size: 34 2927 34 3678 Fragments arranged by size: 3678 2927 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4565 5775 4565 Size: 1210 5463 BclI T'GATC_A Cuts at: 1548 1548 Size: 6673 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2713 5284 136 Size: 107 122 71 631 605 1041 2571 1525 Fragments arranged by size: 2571 1525 1041 631 605 122 107 71 BglII A'GATC_T Cuts at: 3453 6668 3453 Size: 3215 3458 BlpI GC'TnA_GC Cuts at: 4090 4090 Size: 6673 Bme1580I G_kGCm'C Cuts at: 1790 2591 2641 3138 3325 3477 3653 3704 4734 Size: 801 50 497 187 152 176 51 1030 Cuts at: 4734 5890 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1030 801 497 187 176 152 51 50 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6673 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3118 4404 5362 Size: 416 53 83 399 24 1701 1286 958 Cuts at: 5362 442 Size: 1753 Fragments arranged by size: 1753 1701 1286 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6673 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2857 2857 Size: 6673 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2019 3623 3943 5333 1239 Size: 780 1604 320 1390 2579 Fragments arranged by size: 2579 1604 1390 780 320 Bpu10I CC'TnA_GC Cuts at: 2832 2832 Size: 6673 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3386 4798 5663 5961 3386 Size: 1412 865 298 4098 Fragments arranged by size: 4098 1412 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5336 882 Size: 617 3837 2219 Fragments arranged by size: 3837 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3383 3491 3713 4464 493 Size: 711 2179 108 222 751 2702 Fragments arranged by size: 2702 2179 751 711 222 108 BsaBI GATnn'nnATC Cuts at: 6667 6667 Size: 6673 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2684 2789 3542 4480 Size: 53 83 186 936 1151 105 753 938 Cuts at: 4480 4862 5497 275 Size: 382 635 1451 Fragments arranged by size: 1451 1151 938 936 753 635 382 186 105 83 53 BsaWI w'CCGG_w Cuts at: 2104 2169 5106 5504 5778 5925 6336 2104 Size: 65 2937 398 274 147 411 2441 Fragments arranged by size: 2937 2441 411 398 274 147 65 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2996 3026 3941 3971 1178 Size: 30 165 30 1593 30 915 30 3880 Fragments arranged by size: 3880 1593 915 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3044 3428 1186 Size: 1858 384 4431 Fragments arranged by size: 4431 1858 384 BseYI C'CCAG_C Cuts at: 2456 3156 3161 3231 3780 5876 6372 2456 Size: 700 5 70 549 2096 496 2757 Fragments arranged by size: 2757 2096 700 549 496 70 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2242 2910 3899 1163 Size: 573 506 668 989 3937 Fragments arranged by size: 3937 989 668 573 506 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3970 4123 4884 5912 1540 Size: 275 186 1969 153 761 1028 2301 Fragments arranged by size: 2301 1969 1028 761 275 186 153 BsiHKAI G_wGCw'C Cuts at: 3279 3660 3729 4734 4819 5890 3279 Size: 381 69 1005 85 1071 4062 Fragments arranged by size: 4062 1071 1005 381 85 69 BsmI GAATG_Cn' Cuts at: 2080 3300 4184 4277 2080 Size: 1220 884 93 4476 Fragments arranged by size: 4476 1220 884 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2310 2411 2591 2641 3138 3260 3279 Size: 399 520 101 180 50 497 122 19 Cuts at: 3279 3325 3477 3653 3660 3704 3729 3992 4734 Size: 46 152 176 7 44 25 263 742 Cuts at: 4734 4819 5890 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 520 497 399 263 180 176 152 122 101 85 50 46 44 25 19 7 BspEI T'CCGG_A Cuts at: 2169 6336 2169 Size: 4167 2506 BspHI T'CATG_A Cuts at: 2121 4562 6292 2121 Size: 2441 1730 2502 Fragments arranged by size: 2502 2441 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2364 3456 3960 4028 844 Size: 1520 1092 504 68 3489 Fragments arranged by size: 3489 1520 1092 504 68 BsrBI CCG'CTC Cuts at: 1394 1823 2278 2293 4560 1394 Size: 429 455 15 2267 3507 Fragments arranged by size: 3507 2267 455 429 15 BsrDI GCAATG_nn' Cuts at: 65 1248 2149 2293 3264 3871 5168 5342 65 Size: 1183 901 144 971 607 1297 174 1396 Fragments arranged by size: 1396 1297 1183 971 901 607 174 144 BsrFI r'CCGG_y Cuts at: 1700 1810 2613 2712 5317 5504 1700 Size: 110 803 99 2605 187 2869 Fragments arranged by size: 2869 2605 803 187 110 99 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6673 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6673 BssSI C'ACGA_G Cuts at: 4733 5745 4733 Size: 1012 5661 BstAPI GCAn_nnn'nTGC Cuts at: 794 3400 794 Size: 2606 4067 BstBI TT'CG_AA Cuts at: 5477 5477 Size: 6673 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6673 BstYI r'GATC_y Cuts at: 1972 2598 2863 3222 3453 4003 4370 4755 4772 Size: 626 265 359 231 550 367 385 17 Cuts at: 4772 6224 6310 6322 6668 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 626 550 385 367 359 346 265 231 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2814 2814 Size: 6673 BtgI C'CryG_G Cuts at: 513 1065 2004 2155 2908 513 Size: 552 939 151 753 4278 Fragments arranged by size: 4278 939 753 552 151 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5934 Fragments arranged by size: 5934 683 56 BtsI GCAGTG_nn' Cuts at: 2308 2717 2790 2982 3116 3251 4189 4983 5003 Size: 409 73 192 134 135 938 794 20 Cuts at: 5003 2308 Size: 3978 Fragments arranged by size: 3978 938 794 409 192 135 134 73 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6673 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3553 3588 529 Size: 35 2989 35 3614 Fragments arranged by size: 3614 2989 35 35 DraI TTT'AAA Cuts at: 3977 3977 Size: 6673 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5680 809 Size: 507 4364 1802 Fragments arranged by size: 4364 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2448 2714 Size: 54 1425 50 275 18 357 261 266 Cuts at: 2714 4120 5009 6638 8 Size: 1406 889 1629 43 Fragments arranged by size: 1629 1425 1406 889 357 275 266 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4120 1537 Size: 275 2308 4090 Fragments arranged by size: 4090 2308 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2931 2963 3267 3596 4603 1353 Size: 1578 32 304 329 1007 3423 Fragments arranged by size: 3423 1578 1007 329 304 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5260 5634 5780 387 Size: 1064 138 3671 374 146 1280 Fragments arranged by size: 3671 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3727 3727 Size: 6673 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2019 3291 3320 3623 3943 4736 5333 Size: 393 387 1272 29 303 320 793 597 Cuts at: 5333 6120 6388 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1272 793 787 597 393 387 320 303 268 29 EcoNI CCTnn'n_nnAGG Cuts at: 2876 2876 Size: 6673 EcoO109I rG'GnC_Cy Cuts at: 2587 2588 2706 2851 2895 3106 3134 3667 2587 Size: 1 118 145 44 211 28 533 5593 Fragments arranged by size: 5593 533 211 145 118 44 28 1 EcoRI G'AATT_C Cuts at: 3982 4360 3982 Size: 378 6295 FspI TGC'GCA Cuts at: 3872 5179 3872 Size: 1307 5366 FspAI rTGC'GCAy Cuts at: 3872 3872 Size: 6673 HaeII r_GCGC'y Cuts at: 1678 2365 3517 3760 5820 1678 Size: 687 1152 243 2060 2531 Fragments arranged by size: 2531 2060 1152 687 243 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 2239 2271 2996 Size: 32 135 32 470 32 165 32 725 Cuts at: 2996 3028 3098 3130 3941 3973 5314 5346 5388 Size: 32 70 32 811 32 1341 32 42 Cuts at: 5388 5420 1373 Size: 32 2626 Fragments arranged by size: 2626 1341 811 725 470 165 135 70 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4017 4263 669 Size: 988 281 2079 246 3079 Fragments arranged by size: 3079 2079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6673 HpaI GTT'AAC Cuts at: 4263 4263 Size: 6673 KpnI G_GTAC'C Cuts at: 3998 3998 Size: 6673 MfeI C'AATT_G Cuts at: 4272 4272 Size: 6673 MluI A'CGCG_T Cuts at: 6549 6549 Size: 6673 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5787 5971 1305 Size: 4482 184 2007 Fragments arranged by size: 4482 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2189 2450 10 Size: 54 1425 343 357 261 4233 Fragments arranged by size: 4233 1425 357 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 3878 4631 4990 5149 518 Size: 648 2712 753 359 159 2042 Fragments arranged by size: 2712 2042 753 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2632 3683 4089 4766 5914 6159 1606 Size: 1026 1051 406 677 1148 245 2120 Fragments arranged by size: 2120 1148 1051 1026 677 406 245 NaeI GCC'GGC Cuts at: 2615 2714 2615 Size: 99 6574 NcoI C'CATG_G Cuts at: 513 1065 2004 2155 2908 513 Size: 552 939 151 753 4278 Fragments arranged by size: 4278 939 753 552 151 NdeI CA'TA_TG Cuts at: 387 387 Size: 6673 NgoMIV G'CCGG_C Cuts at: 2613 2712 2613 Size: 99 6574 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6673 NotI GC'GGCC_GC Cuts at: 4120 4120 Size: 6673 NspI r_CATG'y Cuts at: 1132 1229 2509 2529 2993 4031 5576 1132 Size: 97 1280 20 464 1038 1545 2229 Fragments arranged by size: 2229 1545 1280 1038 464 97 20 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6673 PciI A'CATG_T Cuts at: 5572 5572 Size: 6673 PflMI CCAn_nnn'nTGG Cuts at: 1108 3130 1108 Size: 2022 4651 PfoI T'CCnGG_A Cuts at: 2199 2949 2199 Size: 750 5923 PmeI GTTT'AAAC Cuts at: 3977 3977 Size: 6673 PmlI CAC'GTG Cuts at: 3491 3491 Size: 6673 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4742 4774 6255 6287 4742 Size: 32 1481 32 5128 Fragments arranged by size: 5128 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2851 3106 3667 2851 Size: 255 561 5857 Fragments arranged by size: 5857 561 255 PshAI GACnn'nnGTC Cuts at: 1613 2547 1613 Size: 934 5739 PsiI TTA'TAA Cuts at: 4243 4243 Size: 6673 PspOMI G'GGCC_C Cuts at: 2587 2637 3134 2587 Size: 50 497 6126 Fragments arranged by size: 6126 497 50 PstI C_TGCA'G Cuts at: 830 2361 3453 4025 830 Size: 1531 1092 572 3478 Fragments arranged by size: 3478 1531 1092 572 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6673 PvuII CAG'CTG Cuts at: 2632 3683 2632 Size: 1051 5622 SacI G_AGCT'C Cuts at: 3729 3729 Size: 6673 SalI G'TCGA_C Cuts at: 1936 4015 1936 Size: 2079 4594 SanDI GG'GwC_CC Cuts at: 3667 3667 Size: 6673 SapI GCTCTTCn'nnn_ Cuts at: 3267 3267 Size: 6673 SbfI CC_TGCA'GG Cuts at: 4025 4025 Size: 6673 ScaI AGT'ACT Cuts at: 2657 4921 6633 2657 Size: 2264 1712 2697 Fragments arranged by size: 2697 2264 1712 SfcI C'TryA_G Cuts at: 826 1045 2357 2820 3449 4021 5156 5837 6028 Size: 219 1312 463 629 572 1135 681 191 Cuts at: 6028 826 Size: 1471 Fragments arranged by size: 1471 1312 1135 681 629 572 463 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2713 2713 Size: 6673 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6673 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6673 SmaI CCC'GGG Cuts at: 1872 2312 2642 4000 1872 Size: 440 330 1358 4545 Fragments arranged by size: 4545 1358 440 330 SmlI C'TyrA_G Cuts at: 820 1017 1943 3365 4777 5678 5940 820 Size: 197 926 1422 1412 901 262 1553 Fragments arranged by size: 1553 1422 1412 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4464 493 Size: 3971 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6673 SphI G_CATG'C Cuts at: 1132 4031 1132 Size: 2899 3774 SspI AAT'ATT Cuts at: 5 52 4597 5 Size: 47 4545 2081 Fragments arranged by size: 4545 2081 47 StuI AGG'CCT Cuts at: 1517 2338 1517 Size: 821 5852 StyI C'CwwG_G Cuts at: 513 1065 2004 2155 2190 2442 2702 2908 3102 Size: 552 939 151 35 252 260 206 194 Cuts at: 3102 4112 513 Size: 1010 3074 Fragments arranged by size: 3074 1010 939 552 260 252 206 194 151 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2470 2741 3099 4700 4717 4870 5055 Size: 1255 890 271 358 1601 17 153 185 Cuts at: 5055 5500 325 Size: 445 1498 Fragments arranged by size: 1601 1498 1255 890 445 358 271 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2655 4919 6631 Size: 274 80 33 51 1443 678 2264 1712 Cuts at: 6631 96 Size: 138 Fragments arranged by size: 2264 1712 1443 678 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6633 6665 6633 Size: 32 6641 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6673 XbaI T'CTAG_A Cuts at: 4009 4009 Size: 6673 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3545 3545 Size: 6673 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6673 XmaI C'CCGG_G Cuts at: 1870 2310 2640 3998 1870 Size: 440 330 1358 4545 Fragments arranged by size: 4545 1358 440 330 XmnI GAAnn'nnTTC Cuts at: 2579 3300 4802 2579 Size: 721 1502 4450 Fragments arranged by size: 4450 1502 721 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3543 4481 276 Size: 53 83 186 936 2009 938 2468 Fragments arranged by size: 2468 2009 938 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI