(Circular) (Six-base) MAPSORT of: pFN21AB5319.seq Check: 4434 from: 1 to: 5983 pFN21AB5319 5983 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2712 3326 1937 Size: 775 614 4594 Fragments arranged by size: 4594 775 614 Acc65I G'GTAC_C Cuts at: 3304 3304 Size: 5983 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2147 2279 4046 5430 5698 1632 Size: 515 132 1767 1384 268 1917 Fragments arranged by size: 1917 1767 1384 515 268 132 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5786 AflIII A'CryG_T Cuts at: 1203 4882 5859 1203 Size: 3679 977 1327 Fragments arranged by size: 3679 1327 977 AgeI A'CCGG_T Cuts at: 4814 4814 Size: 5983 AhdI GACnn_n'nnGTC Cuts at: 4712 4712 Size: 5983 AleI CACnn'nnGTG Cuts at: 1166 2765 1166 Size: 1599 4384 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2966 1184 Size: 1782 4201 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2871 2903 2871 Size: 32 5951 AlwNI CAG_nnn'CTG Cuts at: 3226 3726 5298 3226 Size: 500 1572 3911 Fragments arranged by size: 3911 1572 500 ApaLI G'TGCA_C Cuts at: 4040 5196 4040 Size: 1156 4827 ApoI r'AATT_y Cuts at: 1493 2845 3030 3047 3292 3518 3670 5779 1493 Size: 1352 185 17 245 226 152 2109 1697 Fragments arranged by size: 2109 1697 1352 245 226 185 152 17 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 3308 1870 Size: 73 1365 4545 Fragments arranged by size: 4545 1365 73 BamHI G'GATC_C Cuts at: 3313 3680 3313 Size: 367 5616 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 3304 4759 618 Size: 325 217 627 354 1163 1455 1842 Fragments arranged by size: 1842 1455 1163 627 354 325 217 BanII G_rGCy'C Cuts at: 1391 2724 3302 1391 Size: 1333 578 4072 Fragments arranged by size: 4072 1333 578 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5012 BbvCI CC'TCA_GC Cuts at: 2448 2448 Size: 5983 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4174 4208 1903 Size: 34 2237 34 3678 Fragments arranged by size: 3678 2237 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3875 5085 3875 Size: 1210 4773 BclI T'GATC_A Cuts at: 1548 1548 Size: 5983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4594 136 Size: 107 122 71 631 605 2922 1525 Fragments arranged by size: 2922 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5978 5978 Size: 5983 BlpI GC'TnA_GC Cuts at: 3400 3400 Size: 5983 Bme1580I G_kGCm'C Cuts at: 1790 4044 5200 1790 Size: 2254 1156 2573 Fragments arranged by size: 2573 2254 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5983 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3714 4672 442 Size: 416 53 83 399 24 2297 958 1753 Fragments arranged by size: 2297 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3203 4643 1239 Size: 1964 1440 2579 Fragments arranged by size: 2579 1964 1440 Bpu10I CC'TnA_GC Cuts at: 2448 2448 Size: 5983 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2188 4108 4973 5271 2188 Size: 1920 865 298 2900 Fragments arranged by size: 2900 1920 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4646 882 Size: 617 3147 2219 Fragments arranged by size: 3147 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3125 3774 493 Size: 711 1921 649 2702 Fragments arranged by size: 2702 1921 711 649 BsaBI GATnn'nnATC Cuts at: 5977 5977 Size: 5983 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3790 4172 4807 275 Size: 53 83 186 936 2257 382 635 1451 Fragments arranged by size: 2257 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2241 2841 4416 4814 5088 5235 5646 2241 Size: 600 1575 398 274 147 411 2578 Fragments arranged by size: 2578 1575 600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2898 2928 1178 Size: 30 165 30 1495 30 4233 Fragments arranged by size: 4233 1495 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2746 1186 Size: 1560 4423 BseYI C'CCAG_C Cuts at: 3144 5186 5682 3144 Size: 2042 496 3445 Fragments arranged by size: 3445 2042 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3191 1163 Size: 573 1455 3955 Fragments arranged by size: 3955 1455 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3433 4194 5222 1540 Size: 275 186 1432 761 1028 2301 Fragments arranged by size: 2301 1432 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2705 2724 4044 4129 5200 2705 Size: 19 1320 85 1071 3488 Fragments arranged by size: 3488 1320 1071 85 19 BsmI GAATG_Cn' Cuts at: 2330 2930 3494 3587 2330 Size: 600 564 93 4726 Fragments arranged by size: 4726 600 564 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2705 2724 3302 4044 4129 5200 1391 Size: 399 915 19 578 742 85 1071 2174 Fragments arranged by size: 2174 1071 915 742 578 399 85 19 BspEI T'CCGG_A Cuts at: 5646 5646 Size: 5983 BspHI T'CATG_A Cuts at: 3872 5602 3872 Size: 1730 4253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3338 844 Size: 2494 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2211 3870 1394 Size: 429 388 1659 3507 Fragments arranged by size: 3507 1659 429 388 BsrDI GCAATG_nn' Cuts at: 65 1248 4478 4652 65 Size: 1183 3230 174 1396 Fragments arranged by size: 3230 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4627 4814 1700 Size: 110 2817 187 2869 Fragments arranged by size: 2869 2817 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5983 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5983 BssSI C'ACGA_G Cuts at: 4043 5055 4043 Size: 1012 4971 BstAPI GCAn_nnn'nTGC Cuts at: 794 3186 794 Size: 2392 3591 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5983 BstXI CCAn_nnnn'nTGG Cuts at: 2697 2697 Size: 5983 BstYI r'GATC_y Cuts at: 1972 3006 3313 3680 4065 4082 5534 5620 5632 Size: 1034 307 367 385 17 1452 86 12 Cuts at: 5632 5978 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1034 385 367 346 307 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2145 3079 513 Size: 552 1080 934 3417 Fragments arranged by size: 3417 1080 934 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2088 487 Size: 683 56 862 4382 Fragments arranged by size: 4382 862 683 56 BtsI GCAGTG_nn' Cuts at: 3499 4293 4313 3499 Size: 794 20 5169 Fragments arranged by size: 5169 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5948 DraI TTT'AAA Cuts at: 3287 3287 Size: 5983 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4990 809 Size: 507 3674 1802 Fragments arranged by size: 3674 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3430 4319 5948 Size: 54 1425 50 275 18 1600 889 1629 Cuts at: 5948 8 Size: 43 Fragments arranged by size: 1629 1600 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3430 1537 Size: 275 1618 4090 Fragments arranged by size: 4090 1618 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 3913 1353 Size: 689 1871 3423 Fragments arranged by size: 3423 1871 689 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4570 4944 5090 387 Size: 1064 138 2981 374 146 1280 Fragments arranged by size: 2981 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2722 2722 Size: 5983 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2279 3203 4046 4643 5430 5698 Size: 393 515 132 924 843 597 787 268 Cuts at: 5698 1239 Size: 1524 Fragments arranged by size: 1524 924 843 787 597 515 393 268 132 EcoRI G'AATT_C Cuts at: 2845 3292 3670 2845 Size: 447 378 5158 Fragments arranged by size: 5158 447 378 FspI TGC'GCA Cuts at: 4489 4489 Size: 5983 HaeII r_GCGC'y Cuts at: 1678 2284 5130 1678 Size: 606 2846 2531 Fragments arranged by size: 2846 2531 606 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4624 4656 4698 4730 1373 Size: 32 135 32 3052 32 42 32 2626 Fragments arranged by size: 3052 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2839 3327 3573 669 Size: 988 281 901 488 246 3079 Fragments arranged by size: 3079 988 901 488 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5983 HpaI GTT'AAC Cuts at: 2839 3573 2839 Size: 734 5249 KpnI G_GTAC'C Cuts at: 3308 3308 Size: 5983 MfeI C'AATT_G Cuts at: 3582 3582 Size: 5983 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2820 2884 2939 5097 5281 1305 Size: 1515 64 55 2158 184 2007 Fragments arranged by size: 2158 2007 1515 184 64 55 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4161 Fragments arranged by size: 4161 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2323 2765 2948 3941 4300 4459 Size: 648 843 314 442 183 993 359 159 Cuts at: 4459 518 Size: 2042 Fragments arranged by size: 2042 993 843 648 442 359 314 183 159 MspA1I CmG'CkG Cuts at: 1606 2042 3399 4076 5224 5469 1606 Size: 436 1357 677 1148 245 2120 Fragments arranged by size: 2120 1357 1148 677 436 245 NcoI C'CATG_G Cuts at: 513 1065 2145 3079 513 Size: 552 1080 934 3417 Fragments arranged by size: 3417 1080 934 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5983 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 2503 3341 4886 1132 Size: 97 1274 838 1545 2229 Fragments arranged by size: 2229 1545 1274 838 97 PasI CC'CwG_GG Cuts at: 1348 2177 1348 Size: 829 5154 PciI A'CATG_T Cuts at: 4882 4882 Size: 5983 PflMI CCAn_nnn'nTGG Cuts at: 1108 3226 1108 Size: 2118 3865 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4052 4084 5565 5597 4052 Size: 32 1481 32 4438 Fragments arranged by size: 4438 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5983 PsiI TTA'TAA Cuts at: 3553 3553 Size: 5983 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2954 2986 2954 Size: 32 5951 PstI C_TGCA'G Cuts at: 830 3335 830 Size: 2505 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5983 PvuII CAG'CTG Cuts at: 2042 2042 Size: 5983 SacI G_AGCT'C Cuts at: 2724 2724 Size: 5983 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SbfI CC_TGCA'GG Cuts at: 3335 3335 Size: 5983 ScaI AGT'ACT Cuts at: 4231 5943 4231 Size: 1712 4271 SfcI C'TryA_G Cuts at: 826 1045 3331 4466 5147 5338 826 Size: 219 2286 1135 681 191 1471 Fragments arranged by size: 2286 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5983 SmaI CCC'GGG Cuts at: 1872 3310 1872 Size: 1438 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2167 4087 4988 5250 820 Size: 197 926 224 1920 901 262 1553 Fragments arranged by size: 1920 1553 926 901 262 224 197 SnaBI TAC'GTA Cuts at: 493 3125 3774 493 Size: 2632 649 2702 Fragments arranged by size: 2702 2632 649 SpeI A'CTAG_T Cuts at: 152 152 Size: 5983 SphI G_CATG'C Cuts at: 1132 3341 1132 Size: 2209 3774 SspI AAT'ATT Cuts at: 5 52 3907 5 Size: 47 3855 2081 Fragments arranged by size: 3855 2081 47 StuI AGG'CCT Cuts at: 1517 2165 1517 Size: 648 5335 StyI C'CwwG_G Cuts at: 513 1065 2145 2818 3079 3422 513 Size: 552 1080 673 261 343 3074 Fragments arranged by size: 3074 1080 673 552 343 261 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2049 2726 3006 4010 4027 4180 4365 Size: 1255 469 677 280 1004 17 153 185 Cuts at: 4365 4810 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1004 677 469 445 280 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2404 3116 4229 Size: 274 80 33 51 1443 427 712 1113 Cuts at: 4229 5941 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1113 712 427 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2898 2930 5943 5975 2898 Size: 32 3013 32 2906 Fragments arranged by size: 3013 2906 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5983 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2152 2767 2152 Size: 615 5368 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5983 XmaI C'CCGG_G Cuts at: 1870 3308 1870 Size: 1438 4545 XmnI GAAnn'nnTTC Cuts at: 2810 4112 2810 Size: 1302 4681 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI