Restriction map for
pFN21AB5228
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AlwI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I ClaI CpoI
DpnII DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI MaeII MamI MboI
MfeI MflI MroI MseI MunI
NarI NcoI NdeI NdeII NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI SapI
Sau3AI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SspBI
SspI SwaI TfiI Tru9I Tth111I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 9 GGCGTC G[GA]^CG[CT]C
AflIII 1083 ACGCGT A^C[GA][CT]GT
Alw44I 649 GTGCAC G^TGCAC
AocI 762 CCTGAGG CC^T[ACGT]AGG
Aor51HI 717 AGCGCT AGC^GCT
ApaLI 649 GTGCAC G^TGCAC
AspHI 649 GTGCAC G[AT]GC[AT]^C
BalI 932 TGGCCA TGG^CCA
BbsI 1049 GAAGAC GAAGAC(2/6)
BbvCI 333 CCTCAGC CCTCAGC(-5/-2)
BfaI 901 CTAG C^TAG
BlpI 1171 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1171 GCTGAGC GC^T[ACGT]AGC
BpuAI 1049 GAAGAC GAAGAC(2/6)
BsaHI 9 GGCGTC G[GA]^CG[CT]C
BsaWI 353 TCCGGT [AT]^CCGG[AT]
BsiEI 612 CGACCG CG[GA][CT]^CG
BsiHKAI 649 GTGCAC G[AT]GC[AT]^C
BsmAI 617 GTCTC GTCTC(1/5)
BsmBI 616 CGTCTC CGTCTC(1/5)
BspHI 683 TCATGA T^CATGA
BsrBI 1180 CCGCTC CCGCTC(-3/-3)
BsrDI 1067 GCAATG GCAATG(2/0)
BssHII 585 GCGCGC G^CGCGC
Bsu36I 762 CCTGAGG CC^T[ACGT]AGG
CelII 1171 GCTGAGC GC^T[ACGT]AGC
Eco47III 717 AGCGCT AGC^GCT
Eco81I 762 CCTGAGG CC^T[ACGT]AGG
Hin1I 9 GGCGTC G[GA]^CG[CT]C
MaeI 901 CTAG C^TAG
MluI 1083 ACGCGT A^CGCGT
MluNI 932 TGGCCA TGG^CCA
MscI 932 TGGCCA TGG^CCA
NaeI 26 GCCGGC GCC^GGC
NgoMIV 26 GCCGGC G^CCGGC
RcaI 683 TCATGA T^CATGA
ScaI 1035 AGTACT AGT^ACT
SmaI 438 CCCGGG CCC^GGG
SphI 657 GCATGC GCATG^C
Tsp509I 285 AATT ^AATT
XmaCI 438 CCCGGG C^CCGGG
XmaI 438 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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EarI 547 CTCTTC CTCTTC(1/4)
3 GAAGAG
EcoT14I 110 CCAAGG C^C[AT][AT]GG
672 CCTTGG
HaeII 717 AGCGCT [GA]GCGC^[CT]
1386 GGCGCT
HgaI 1082 GACGC GACGC(5/10)
10 GCGTC
Ksp632I 547 CTCTTC CTCTTC(1/4)
3 GAAGAG
KspI 450 CCGCGG CCGC^GG
834 CCGCGG
NspI 210 GCATGT [GA]CATG^[CT]
657 GCATGC
PflMI 251 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
378 CCACGGCCTGG
PvuII 218 CAGCTG CAG^CTG
325 CAGCTG
SacII 450 CCGCGG CCGC^GG
834 CCGCGG
StuI 56 AGGCCT AGG^CCT
1028 AGGCCT
StyI 110 CCAAGG C^C[AT][AT]GG
672 CCTTGG
TaqI 268 TCGA T^CGA
611 TCGA
TthHB8I 268 TCGA T^CGA
611 TCGA
Van91I 251 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
378 CCACGGCCTGG
XcmI 725 CCACTGAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
808 CCACTCTGGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
PstI RsaI Sau96I SbfI ScrFI
SfaNI SfcI Sse8387I TseI Tsp45I
TspRI