(Circular) (Six-base) MAPSORT of: pFN21AB5228.seq Check: 1035 from: 1 to: 6124 pFN21AB5228 6124 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2059 3374 2059 Size: 1315 4809 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3934 278 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3467 1937 Size: 1530 4594 Acc65I G'GTAC_C Cuts at: 3445 3445 Size: 6124 AclI AA'CG_TT Cuts at: 1563 4251 4624 1563 Size: 2688 373 3063 Fragments arranged by size: 3063 2688 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3101 4187 5571 5839 1632 Size: 1469 1086 1384 268 1917 Fragments arranged by size: 1917 1469 1384 1086 268 AfeI AGC'GCT Cuts at: 2724 2724 Size: 6124 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5927 AflIII A'CryG_T Cuts at: 1203 3088 5023 6000 1203 Size: 1885 1935 977 1327 Fragments arranged by size: 1935 1885 1327 977 AgeI A'CCGG_T Cuts at: 4955 4955 Size: 6124 AhdI GACnn_n'nnGTC Cuts at: 4853 4853 Size: 6124 AleI CACnn'nnGTG Cuts at: 1166 3194 1166 Size: 2028 4096 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2098 2210 2679 1184 Size: 914 112 469 4629 Fragments arranged by size: 4629 914 469 112 AlwNI CAG_nnn'CTG Cuts at: 2146 2437 2509 2767 3288 3867 5439 2146 Size: 291 72 258 521 579 1572 2831 Fragments arranged by size: 2831 1572 579 521 291 258 72 ApaI G_GGCC'C Cuts at: 2444 3242 3420 2444 Size: 798 178 5148 Fragments arranged by size: 5148 798 178 ApaLI G'TGCA_C Cuts at: 2654 4181 5337 2654 Size: 1527 1156 3441 Fragments arranged by size: 3441 1527 1156 ApoI r'AATT_y Cuts at: 1493 3433 3659 3811 5920 1493 Size: 1940 226 152 2109 1697 Fragments arranged by size: 2109 1940 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4678 160 Size: 872 3646 1606 Fragments arranged by size: 3646 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 AvaI C'yCGr_G Cuts at: 1870 1943 2443 2585 3203 3235 3449 1870 Size: 73 500 142 618 32 214 4545 Fragments arranged by size: 4545 618 500 214 142 73 32 BamHI G'GATC_C Cuts at: 3454 3821 3454 Size: 367 5757 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2857 3347 3445 4900 Size: 325 217 627 303 767 490 98 1455 Cuts at: 4900 618 Size: 1842 Fragments arranged by size: 1842 1455 767 627 490 325 303 217 98 BanII G_rGCy'C Cuts at: 1391 2115 2444 3183 3212 3242 3420 3443 1391 Size: 724 329 739 29 30 178 23 4072 Fragments arranged by size: 4072 739 724 329 178 30 29 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3061 928 Size: 971 1162 3991 Fragments arranged by size: 3991 1162 971 BbvCI CC'TCA_GC Cuts at: 2339 2339 Size: 6124 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4315 4349 1903 Size: 34 2378 34 3678 Fragments arranged by size: 3678 2378 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4016 5226 4016 Size: 1210 4914 BclI T'GATC_A Cuts at: 1548 1548 Size: 6124 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2886 2995 3114 Size: 107 122 71 631 605 1214 109 119 Cuts at: 3114 3297 3306 4735 136 Size: 183 9 1429 1525 Fragments arranged by size: 1525 1429 1214 631 605 183 122 119 109 107 71 9 BglII A'GATC_T Cuts at: 6119 6119 Size: 6124 BlpI GC'TnA_GC Cuts at: 3177 3541 3177 Size: 364 5760 Bme1580I G_kGCm'C Cuts at: 1790 2160 2444 2586 2658 2758 2809 2862 3242 Size: 370 284 142 72 100 51 53 380 Cuts at: 3242 3352 3420 4185 5341 1790 Size: 110 68 765 1156 2573 Fragments arranged by size: 2573 1156 765 380 370 284 142 110 100 72 68 53 51 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3855 4813 442 Size: 416 53 83 399 24 2438 958 1753 Fragments arranged by size: 2438 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6124 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2043 2345 2560 3065 4784 1239 Size: 804 302 215 505 1719 2579 Fragments arranged by size: 2579 1719 804 505 302 215 Bpu10I CC'TnA_GC Cuts at: 2339 3343 2339 Size: 1004 5120 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4249 5114 5412 4249 Size: 865 298 4961 Fragments arranged by size: 4961 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4787 882 Size: 617 3288 2219 Fragments arranged by size: 3288 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3915 493 Size: 711 2711 2702 Fragments arranged by size: 2711 2702 711 BsaBI GATnn'nnATC Cuts at: 6118 6118 Size: 6124 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 3931 4313 4948 Size: 53 83 186 936 482 1916 382 635 Cuts at: 4948 275 Size: 1451 Fragments arranged by size: 1916 1451 936 635 482 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2358 4557 4955 5229 5376 5787 2358 Size: 2199 398 274 147 411 2695 Fragments arranged by size: 2695 2199 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2558 2588 1178 Size: 30 165 30 1155 30 4714 Fragments arranged by size: 4714 1155 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2836 2839 2999 1186 Size: 1650 3 160 4311 Fragments arranged by size: 4311 1650 160 3 BseYI C'CCAG_C Cuts at: 2136 2437 2992 3356 5327 5823 2136 Size: 301 555 364 1971 496 2437 Fragments arranged by size: 2437 1971 555 496 364 301 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2180 2252 2393 2543 2591 2615 1163 Size: 573 444 72 141 150 48 24 4672 Fragments arranged by size: 4672 573 444 150 141 72 48 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2620 3574 4335 5363 1540 Size: 275 186 619 954 761 1028 2301 Fragments arranged by size: 2301 1028 954 761 619 275 186 BsiHKAI G_wGCw'C Cuts at: 2658 4185 4270 5341 2658 Size: 1527 85 1071 3441 Fragments arranged by size: 3441 1527 1071 85 BsmI GAATG_Cn' Cuts at: 3635 3728 3635 Size: 93 6031 BsmBI CGTCTCn'nnnn_ Cuts at: 2627 2627 Size: 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2160 2444 2586 2658 2758 2809 Size: 399 325 45 284 142 72 100 51 Cuts at: 2809 2862 3183 3212 3242 3352 3420 3443 4185 Size: 53 321 29 30 110 68 23 742 Cuts at: 4185 4270 5341 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 325 321 284 142 110 100 85 72 68 53 51 45 30 29 23 BspEI T'CCGG_A Cuts at: 5787 5787 Size: 6124 BspHI T'CATG_A Cuts at: 2688 4013 5743 2688 Size: 1325 1730 3069 Fragments arranged by size: 3069 1730 1325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2059 2493 2928 3374 3479 844 Size: 1215 434 435 446 105 3489 Fragments arranged by size: 3489 1215 446 435 434 105 BsrBI CCG'CTC Cuts at: 1394 1823 3187 4011 1394 Size: 429 1364 824 3507 Fragments arranged by size: 3507 1364 824 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3079 4619 4793 65 Size: 1183 1831 1540 174 1396 Fragments arranged by size: 1831 1540 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2031 2942 2966 4768 4955 1700 Size: 110 221 911 24 1802 187 2869 Fragments arranged by size: 2869 1802 911 221 187 110 24 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6124 BssHII G'CGCG_C Cuts at: 1925 2590 1925 Size: 665 5459 BssSI C'ACGA_G Cuts at: 4184 5196 4184 Size: 1012 5112 BstAPI GCAn_nnn'nTGC Cuts at: 794 2146 2155 2509 3074 3288 794 Size: 1352 9 354 565 214 3630 Fragments arranged by size: 3630 1352 565 354 214 9 BstBI TT'CG_AA Cuts at: 4928 4928 Size: 6124 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6124 BstYI r'GATC_y Cuts at: 1972 3454 3821 4206 4223 5675 5761 5773 6119 Size: 1482 367 385 17 1452 86 12 346 Cuts at: 6119 1972 Size: 1977 Fragments arranged by size: 1977 1482 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2768 2768 Size: 6124 BtgI C'CryG_G Cuts at: 513 1065 2160 2383 2455 2599 2839 3131 513 Size: 552 1095 223 72 144 240 292 3506 Fragments arranged by size: 3506 1095 552 292 240 223 144 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5385 Fragments arranged by size: 5385 683 56 BtsI GCAGTG_nn' Cuts at: 2507 3189 3201 3286 3640 4434 4454 2507 Size: 682 12 85 354 794 20 4177 Fragments arranged by size: 4177 794 682 354 85 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6089 DraI TTT'AAA Cuts at: 3428 3428 Size: 6124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5131 809 Size: 507 3815 1802 Fragments arranged by size: 3815 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2680 2887 2937 Size: 54 1425 50 275 18 850 207 50 Cuts at: 2937 3571 4460 6089 8 Size: 634 889 1629 43 Fragments arranged by size: 1629 1425 889 850 634 275 207 54 50 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3571 1537 Size: 275 1759 4090 Fragments arranged by size: 4090 1759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2003 2558 4054 1353 Size: 650 555 1496 3423 Fragments arranged by size: 3423 1496 650 555 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4711 5085 5231 387 Size: 1064 138 3122 374 146 1280 Fragments arranged by size: 3122 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2043 2345 2560 3065 3101 4187 4784 Size: 393 411 302 215 505 36 1086 597 Cuts at: 4784 5571 5839 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1086 787 597 505 411 393 302 268 215 36 EcoO109I rG'GnC_Cy Cuts at: 2143 2737 3239 3417 2143 Size: 594 502 178 4850 Fragments arranged by size: 4850 594 502 178 EcoRI G'AATT_C Cuts at: 3433 3811 3433 Size: 378 5746 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3078 3078 Size: 6124 FspI TGC'GCA Cuts at: 4630 4630 Size: 6124 HaeII r_GCGC'y Cuts at: 1678 2726 3395 5271 1678 Size: 1048 669 1876 2531 Fragments arranged by size: 2531 1876 1048 669 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2683 2715 3020 3052 4765 Size: 32 135 32 1111 32 305 32 1713 Cuts at: 4765 4797 4839 4871 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1713 1111 305 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3468 3714 669 Size: 988 281 1530 246 3079 Fragments arranged by size: 3079 1530 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6124 HpaI GTT'AAC Cuts at: 3714 3714 Size: 6124 KpnI G_GTAC'C Cuts at: 3449 3449 Size: 6124 MfeI C'AATT_G Cuts at: 3723 3723 Size: 6124 MluI A'CGCG_T Cuts at: 3088 6000 3088 Size: 2912 3212 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5238 5422 1305 Size: 3933 184 2007 Fragments arranged by size: 3933 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2939 10 Size: 54 1425 343 1107 3195 Fragments arranged by size: 3195 1425 1107 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2274 2661 3141 3194 4082 4441 4600 Size: 648 1108 387 480 53 888 359 159 Cuts at: 4600 518 Size: 2042 Fragments arranged by size: 2042 1108 888 648 480 387 359 159 53 MspA1I CmG'CkG Cuts at: 1606 2021 2225 2332 2421 2457 2841 2992 3026 Size: 415 204 107 89 36 384 151 34 Cuts at: 3026 3312 3540 4217 5365 5610 1606 Size: 286 228 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 415 384 286 245 228 204 151 107 89 36 34 NaeI GCC'GGC Cuts at: 2033 2033 Size: 6124 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5572 NdeI CA'TA_TG Cuts at: 387 387 Size: 6124 NgoMIV G'CCGG_C Cuts at: 2031 2031 Size: 6124 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6124 NotI GC'GGCC_GC Cuts at: 3571 3571 Size: 6124 NspI r_CATG'y Cuts at: 1132 1229 2219 2666 3482 5027 1132 Size: 97 990 447 816 1545 2229 Fragments arranged by size: 2229 1545 990 816 447 97 PasI CC'CwG_GG Cuts at: 1348 2787 3382 1348 Size: 1439 595 4090 Fragments arranged by size: 4090 1439 595 PciI A'CATG_T Cuts at: 5023 5023 Size: 6124 PflMI CCAn_nnn'nTGG Cuts at: 1108 2262 2389 1108 Size: 1154 127 4843 Fragments arranged by size: 4843 1154 127 PfoI T'CCnGG_A Cuts at: 2322 2322 Size: 6124 PmeI GTTT'AAAC Cuts at: 3428 3428 Size: 6124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4193 4225 5706 5738 4193 Size: 32 1481 32 4579 Fragments arranged by size: 4579 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6124 PsiI TTA'TAA Cuts at: 3694 3694 Size: 6124 PspOMI G'GGCC_C Cuts at: 2440 3238 3416 2440 Size: 798 178 5148 Fragments arranged by size: 5148 798 178 PstI C_TGCA'G Cuts at: 830 2061 2151 2433 2475 2490 2505 2763 2832 Size: 1231 90 282 42 15 15 258 69 Cuts at: 2832 2867 2925 3196 3371 3476 830 Size: 35 58 271 175 105 3478 Fragments arranged by size: 3478 1231 282 271 258 175 105 90 69 58 42 35 15 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6124 PvuII CAG'CTG Cuts at: 2225 2332 2225 Size: 107 6017 SacII CC_GC'GG Cuts at: 2458 2842 2458 Size: 384 5740 SalI G'TCGA_C Cuts at: 1936 3466 1936 Size: 1530 4594 SbfI CC_TGCA'GG Cuts at: 2151 2433 2490 2925 3476 2151 Size: 282 57 435 551 4799 Fragments arranged by size: 4799 551 435 282 57 ScaI AGT'ACT Cuts at: 3042 4372 6084 3042 Size: 1330 1712 3082 Fragments arranged by size: 3082 1712 1330 SfcI C'TryA_G Cuts at: 826 1045 2057 2147 2429 2471 2486 2501 2759 Size: 219 1012 90 282 42 15 15 258 Cuts at: 2759 2828 2863 2921 3192 3367 3472 4607 5288 Size: 69 35 58 271 175 105 1135 681 Cuts at: 5288 5479 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1012 681 282 271 258 219 191 175 105 90 69 58 42 35 15 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6124 SmaI CCC'GGG Cuts at: 1872 2445 3451 1872 Size: 573 1006 4545 Fragments arranged by size: 4545 1006 573 SmlI C'TyrA_G Cuts at: 820 1017 1943 4228 5129 5391 820 Size: 197 926 2285 901 262 1553 Fragments arranged by size: 2285 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3915 493 Size: 3422 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6124 SphI G_CATG'C Cuts at: 1132 2666 3482 1132 Size: 1534 816 3774 Fragments arranged by size: 3774 1534 816 SspI AAT'ATT Cuts at: 5 52 4048 5 Size: 47 3996 2081 Fragments arranged by size: 3996 2081 47 StuI AGG'CCT Cuts at: 1517 2063 3035 1517 Size: 546 972 4606 Fragments arranged by size: 4606 972 546 StyI C'CwwG_G Cuts at: 513 1065 2115 2677 3563 513 Size: 552 1050 562 886 3074 Fragments arranged by size: 3074 1050 886 562 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2035 2779 4151 4168 4321 4506 4951 Size: 1255 455 744 1372 17 153 185 445 Cuts at: 4951 325 Size: 1498 Fragments arranged by size: 1498 1372 1255 744 455 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3040 4370 6082 Size: 274 80 33 51 1443 1063 1330 1712 Cuts at: 6082 96 Size: 138 Fragments arranged by size: 1712 1443 1330 1063 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2538 2570 3210 3242 6084 6116 2538 Size: 32 640 32 2842 32 2546 Fragments arranged by size: 2842 2546 640 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6124 XbaI T'CTAG_A Cuts at: 3460 3460 Size: 6124 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2737 2820 2737 Size: 83 6041 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6124 XmaI C'CCGG_G Cuts at: 1870 2443 3449 1870 Size: 573 1006 4545 Fragments arranged by size: 4545 1006 573 XmnI GAAnn'nnTTC Cuts at: 4253 4253 Size: 6124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3932 276 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI