Restriction map for
pFN21AB5228
(Linear) (Six-base) MAPSORT of: KIBB5228.seq Check: 3489 from: 1 to: 1419
KIBB5228.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 54 1369 1419
Size: 54 1315 50
Fragments arranged by size:
1315 54 50
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1419
Size: 1096 323
AfeI AGC'GCT
Cuts at: 0 719 1419
Size: 719 700
AflIII A'CryG_T
Cuts at: 0 1083 1419
Size: 1083 336
AleI CACnn'nnGTG
Cuts at: 0 1189 1419
Size: 1189 230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 93 205 674 1419
Size: 93 112 469 745
Fragments arranged by size:
745 469 112 93
AlwNI CAG_nnn'CTG
Cuts at: 0 141 432 504 762 1283 1419
Size: 141 291 72 258 521 136
Fragments arranged by size:
521 291 258 141 136 72
ApaI G_GGCC'C
Cuts at: 0 439 1237 1415 1419
Size: 439 798 178 4
Fragments arranged by size:
798 439 178 4
ApaLI G'TGCA_C
Cuts at: 0 649 1419
Size: 649 770
AvaI C'yCGr_G
Cuts at: 0 438 580 1198 1230 1419
Size: 438 142 618 32 189
Fragments arranged by size:
618 438 189 142 32
BanI G'GyrC_C
Cuts at: 0 85 852 1342 1419
Size: 85 767 490 77
Fragments arranged by size:
767 490 85 77
BanII G_rGCy'C
Cuts at: 0 110 439 1178 1207 1237 1415 1419
Size: 110 329 739 29 30 178 4
Fragments arranged by size:
739 329 178 110 30 29 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 1056 1419
Size: 1056 363
BbvCI CC'TCA_GC
Cuts at: 0 334 1419
Size: 334 1085
BglI GCCn_nnn'nGGC
Cuts at: 0 881 990 1109 1292 1301 1419
Size: 881 109 119 183 9 118
Fragments arranged by size:
881 183 119 118 109 9
BlpI GC'TnA_GC
Cuts at: 0 1172 1419
Size: 1172 247
Bme1580I G_kGCm'C
Cuts at: 0 155 439 581 653 753 804 857 1237
Size: 155 284 142 72 100 51 53 380
Cuts at: 1237 1347 1415 1419
Size: 110 68 4
Fragments arranged by size:
380 284 155 142 110 100 72 68
53 51 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 340 555 1060 1419
Size: 38 302 215 505 359
Fragments arranged by size:
505 359 302 215 38
Bpu10I CC'TnA_GC
Cuts at: 0 334 1338 1419
Size: 334 1004 81
Fragments arranged by size:
1004 334 81
BsaHI Gr'CG_yC
Cuts at: 0 10 1419
Size: 10 1409
BsaWI w'CCGG_w
Cuts at: 0 353 1419
Size: 353 1066
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 553 583 1419
Size: 553 30 836
Fragments arranged by size:
836 553 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 831 834 994 1419
Size: 831 3 160 425
Fragments arranged by size:
831 425 160 3
BseYI C'CCAG_C
Cuts at: 0 131 432 987 1351 1419
Size: 131 301 555 364 68
Fragments arranged by size:
555 364 301 131 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 247 388 538 586 610 1419
Size: 175 72 141 150 48 24 809
Fragments arranged by size:
809 175 150 141 72 48 24
BsiEI CG_ry'CG
Cuts at: 0 615 1419
Size: 615 804
BsiHKAI G_wGCw'C
Cuts at: 0 653 1419
Size: 653 766
BsmBI CGTCTCn'nnnn_
Cuts at: 0 622 1419
Size: 622 797
Bsp1286I G_dGCh'C
Cuts at: 0 110 155 439 581 653 753 804 857
Size: 110 45 284 142 72 100 51 53
Cuts at: 857 1178 1207 1237 1347 1415 1419
Size: 321 29 30 110 68 4
Fragments arranged by size:
321 284 142 110 110 100 72 68
53 51 45 30 29 4
BspHI T'CATG_A
Cuts at: 0 683 1419
Size: 683 736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 54 488 923 1369 1419
Size: 54 434 435 446 50
Fragments arranged by size:
446 435 434 54 50
BsrBI CCG'CTC
Cuts at: 0 1182 1419
Size: 1182 237
BsrDI GCAATG_nn'
Cuts at: 0 1074 1419
Size: 1074 345
BsrFI r'CCGG_y
Cuts at: 0 26 937 961 1419
Size: 26 911 24 458
Fragments arranged by size:
911 458 26 24
BssHII G'CGCG_C
Cuts at: 0 585 1419
Size: 585 834
BstAPI GCAn_nnn'nTGC
Cuts at: 0 141 150 504 1069 1283 1419
Size: 141 9 354 565 214 136
Fragments arranged by size:
565 354 214 141 136 9
Bsu36I CC'TnA_GG
Cuts at: 0 763 1419
Size: 763 656
BtgI C'CryG_G
Cuts at: 0 155 378 450 594 834 1126 1419
Size: 155 223 72 144 240 292 293
Fragments arranged by size:
293 292 240 223 155 144 72
BtsI GCAGTG_nn'
Cuts at: 0 502 1184 1196 1281 1419
Size: 502 682 12 85 138
Fragments arranged by size:
682 502 138 85 12
EaeI y'GGCC_r
Cuts at: 0 675 882 932 1419
Size: 675 207 50 487
Fragments arranged by size:
675 487 207 50
EarI CTCTTCn'nnn_
Cuts at: 0 553 1419
Size: 553 866
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 340 555 1060 1096 1419
Size: 38 302 215 505 36 323
Fragments arranged by size:
505 323 302 215 38 36
EcoO109I rG'GnC_Cy
Cuts at: 0 138 732 1234 1412 1419
Size: 138 594 502 178 7
Fragments arranged by size:
594 502 178 138 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1073 1419
Size: 1073 346
HaeII r_GCGC'y
Cuts at: 0 721 1390 1419
Size: 721 669 29
Fragments arranged by size:
721 669 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 678 710 1015 1047 1419
Size: 678 32 305 32 372
Fragments arranged by size:
678 372 305 32 32
MluI A'CGCG_T
Cuts at: 0 1083 1419
Size: 1083 336
MscI TGG'CCA
Cuts at: 0 934 1419
Size: 934 485
MslI CAynn'nnrTG
Cuts at: 0 269 656 1136 1189 1419
Size: 269 387 480 53 230
Fragments arranged by size:
480 387 269 230 53
MspA1I CmG'CkG
Cuts at: 0 16 220 327 416 452 836 987 1021
Size: 16 204 107 89 36 384 151 34
Cuts at: 1021 1307 1419
Size: 286 112
Fragments arranged by size:
384 286 204 151 112 107 89 36
34 16
NaeI GCC'GGC
Cuts at: 0 28 1419
Size: 28 1391
NgoMIV G'CCGG_C
Cuts at: 0 26 1419
Size: 26 1393
NspI r_CATG'y
Cuts at: 0 214 661 1419
Size: 214 447 758
Fragments arranged by size:
758 447 214
PasI CC'CwG_GG
Cuts at: 0 782 1377 1419
Size: 782 595 42
Fragments arranged by size:
782 595 42
PflMI CCAn_nnn'nTGG
Cuts at: 0 257 384 1419
Size: 257 127 1035
Fragments arranged by size:
1035 257 127
PfoI T'CCnGG_A
Cuts at: 0 317 1419
Size: 317 1102
PspOMI G'GGCC_C
Cuts at: 0 435 1233 1411 1419
Size: 435 798 178 8
Fragments arranged by size:
798 435 178 8
PstI C_TGCA'G
Cuts at: 0 56 146 428 470 485 500 758 827
Size: 56 90 282 42 15 15 258 69
Cuts at: 827 862 920 1191 1366 1419
Size: 35 58 271 175 53
Fragments arranged by size:
282 271 258 175 90 69 58 56
53 42 35 15 15
PvuII CAG'CTG
Cuts at: 0 220 327 1419
Size: 220 107 1092
Fragments arranged by size:
1092 220 107
SacII CC_GC'GG
Cuts at: 0 453 837 1419
Size: 453 384 582
Fragments arranged by size:
582 453 384
SbfI CC_TGCA'GG
Cuts at: 0 146 428 485 920 1419
Size: 146 282 57 435 499
Fragments arranged by size:
499 435 282 146 57
ScaI AGT'ACT
Cuts at: 0 1037 1419
Size: 1037 382
SfcI C'TryA_G
Cuts at: 0 52 142 424 466 481 496 754 823
Size: 52 90 282 42 15 15 258 69
Cuts at: 823 858 916 1187 1362 1419
Size: 35 58 271 175 57
Fragments arranged by size:
282 271 258 175 90 69 58 57
52 42 35 15 15
SmaI CCC'GGG
Cuts at: 0 440 1419
Size: 440 979
SphI G_CATG'C
Cuts at: 0 661 1419
Size: 661 758
StuI AGG'CCT
Cuts at: 0 58 1030 1419
Size: 58 972 389
Fragments arranged by size:
972 389 58
StyI C'CwwG_G
Cuts at: 0 110 672 1419
Size: 110 562 747
Fragments arranged by size:
747 562 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 30 774 1419
Size: 30 744 645
Fragments arranged by size:
744 645 30
TatI w'GTAC_w
Cuts at: 0 1035 1419
Size: 1035 384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 533 565 1205 1237 1419
Size: 533 32 640 32 182
Fragments arranged by size:
640 533 182 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 732 815 1419
Size: 732 83 604
Fragments arranged by size:
732 604 83
XmaI C'CCGG_G
Cuts at: 0 438 1419
Size: 438 981
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
ApaLI AvaI BanI BanII BbsI BbvCI BglI BlpI
Bme1580I BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssHII BstAPI Bsu36I BtgI BtsI EaeI
EarI Eco57MI EcoO109I FalI HaeII Hin4I MluI MscI
MslI MspA1I NaeI NgoMIV NspI PasI PflMI PfoI
PspOMI PstI PvuII SacII SbfI ScaI SfcI SmaI
SphI StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsiWI BsmI BspEI
BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MmeI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XhoI XmnI ZraI