Restriction map for
pFN21AB5225
(Linear) (Six-base) MAPSORT of: KIBB5225.seq Check: 9774 from: 1 to: 2103
KIBB5225.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 851 1024 2103
Size: 851 173 1079
Fragments arranged by size:
1079 851 173
AatII G_ACGT'C
Cuts at: 0 368 1824 2103
Size: 368 1456 279
Fragments arranged by size:
1456 368 279
Acc65I G'GTAC_C
Cuts at: 0 1199 2103
Size: 1199 904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 613 979 1141 1562 2103
Size: 592 21 366 162 421 541
Fragments arranged by size:
592 541 421 366 162 21
AflIII A'CryG_T
Cuts at: 0 1427 2103
Size: 1427 676
AleI CACnn'nnGTG
Cuts at: 0 1507 2103
Size: 1507 596
AlwNI CAG_nnn'CTG
Cuts at: 0 114 1606 2103
Size: 114 1492 497
Fragments arranged by size:
1492 497 114
ApaI G_GGCC'C
Cuts at: 0 1137 2103
Size: 1137 966
ApoI r'AATT_y
Cuts at: 0 492 2103
Size: 492 1611
AvaI C'yCGr_G
Cuts at: 0 525 1177 2103
Size: 525 652 926
Fragments arranged by size:
926 652 525
AvrII C'CTAG_G
Cuts at: 0 513 2103
Size: 513 1590
BanI G'GyrC_C
Cuts at: 0 1036 1199 1611 1704 1895 2103
Size: 1036 163 412 93 191 208
Fragments arranged by size:
1036 412 208 191 163 93
BanII G_rGCy'C
Cuts at: 0 730 1137 1848 2022 2103
Size: 730 407 711 174 81
Fragments arranged by size:
730 711 407 174 81
BbeI G_GCGC'C
Cuts at: 0 1708 1899 2103
Size: 1708 191 204
Fragments arranged by size:
1708 204 191
BbsI GAAGACnn'nnnn_
Cuts at: 0 1340 2103
Size: 1340 763
BbvCI CC'TCA_GC
Cuts at: 0 754 1186 1883 2103
Size: 754 432 697 220
Fragments arranged by size:
754 697 432 220
BglI GCCn_nnn'nGGC
Cuts at: 0 130 518 1713 1893 2026 2103
Size: 130 388 1195 180 133 77
Fragments arranged by size:
1195 388 180 133 130 77
BlpI GC'TnA_GC
Cuts at: 0 749 2103
Size: 749 1354
Bme1580I G_kGCm'C
Cuts at: 0 1041 1137 1514 2103
Size: 1041 96 377 589
Fragments arranged by size:
1041 589 377 96
BmrI ACTGGGnnnn_n'
Cuts at: 0 1663 1954 2103
Size: 1663 291 149
Fragments arranged by size:
1663 291 149
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1029 2103
Size: 1029 1074
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 376 418 712 1021 1430 1441 1532
Size: 136 240 42 294 309 409 11 91
Cuts at: 1532 1538 1745 2103
Size: 6 207 358
Fragments arranged by size:
409 358 309 294 240 207 136 91
42 11 6
Bpu10I CC'TnA_GC
Cuts at: 0 754 1186 1883 2103
Size: 754 432 697 220
Fragments arranged by size:
754 697 432 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 952 1027 2103
Size: 448 504 75 1076
Fragments arranged by size:
1076 504 448 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 61 931 1743 2089 2103
Size: 61 870 812 346 14
Fragments arranged by size:
870 812 346 61 14
BsaBI GATnn'nnATC
Cuts at: 0 710 2103
Size: 710 1393
BsaHI Gr'CG_yC
Cuts at: 0 365 1367 1705 1821 1896 2103
Size: 365 1002 338 116 75 207
Fragments arranged by size:
1002 365 338 207 116 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1518 1548 2103
Size: 1518 30 555
Fragments arranged by size:
1518 555 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 409 412 592 595 598 601 1018 1246
Size: 409 3 180 3 3 3 417 228
Cuts at: 1246 1743 2030 2103
Size: 497 287 73
Fragments arranged by size:
497 417 409 287 228 180 73 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 852 943 1258 1325 1518 1566 1924 1954
Size: 852 91 315 67 193 48 358 30
Cuts at: 1954 2052 2103
Size: 98 51
Fragments arranged by size:
852 358 315 193 98 91 67 51
48 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 1182 1964 2103
Size: 1 1181 782 139
Fragments arranged by size:
1181 782 139 1
BsiHKAI G_wGCw'C
Cuts at: 0 730 2103
Size: 730 1373
BsmBI CGTCTCn'nnnn_
Cuts at: 0 559 691 970 2103
Size: 559 132 279 1133
Fragments arranged by size:
1133 559 279 132
Bsp1286I G_dGCh'C
Cuts at: 0 730 1041 1137 1514 1848 2022 2103
Size: 730 311 96 377 334 174 81
Fragments arranged by size:
730 377 334 311 174 96 81
BspHI T'CATG_A
Cuts at: 0 890 2103
Size: 890 1213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 90 101 707 851 1024 2103
Size: 90 11 606 144 173 1079
Fragments arranged by size:
1079 606 173 144 90 11
BsrBI CCG'CTC
Cuts at: 0 182 1751 2103
Size: 182 1569 352
Fragments arranged by size:
1569 352 182
BsrDI GCAATG_nn'
Cuts at: 0 616 2103
Size: 616 1487
BsrFI r'CCGG_y
Cuts at: 0 120 1904 2103
Size: 120 1784 199
Fragments arranged by size:
1784 199 120
BssSI C'ACGA_G
Cuts at: 0 442 2103
Size: 442 1661
BstAPI GCAn_nnn'nTGC
Cuts at: 0 24 2103
Size: 24 2079
BstEII G'GTnAC_C
Cuts at: 0 1638 2103
Size: 1638 465
BstXI CCAn_nnnn'nTGG
Cuts at: 0 240 561 1278 1359 2103
Size: 240 321 717 81 744
Fragments arranged by size:
744 717 321 240 81
Bsu36I CC'TnA_GG
Cuts at: 0 1195 2024 2103
Size: 1195 829 79
Fragments arranged by size:
1195 829 79
BtgI C'CryG_G
Cuts at: 0 548 765 2103
Size: 548 217 1338
Fragments arranged by size:
1338 548 217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 697 1251 2103
Size: 697 554 852
Fragments arranged by size:
852 697 554
BtsI GCAGTG_nn'
Cuts at: 0 1502 2103
Size: 1502 601
DraIII CAC_nnn'GTG
Cuts at: 0 1029 2103
Size: 1029 1074
EaeI y'GGCC_r
Cuts at: 0 122 335 551 768 1226 1297 2103
Size: 122 213 216 217 458 71 806
Fragments arranged by size:
806 458 217 216 213 122 71
EarI CTCTTCn'nnn_
Cuts at: 0 1 589 914 934 1408 1444 2103
Size: 1 588 325 20 474 36 659
Fragments arranged by size:
659 588 474 325 36 20 1
EcoICRI GAG'CTC
Cuts at: 0 728 2103
Size: 728 1375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 376 418 592 613 712 979 1021
Size: 136 240 42 174 21 99 267 42
Cuts at: 1021 1141 1430 1441 1532 1538 1562 1745 2103
Size: 120 289 11 91 6 24 183 358
Fragments arranged by size:
358 289 267 240 183 174 136 120
99 91 42 42 24 21 11 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 85 97 1153 2103
Size: 85 12 1056 950
Fragments arranged by size:
1056 950 85 12
EcoO109I rG'GnC_Cy
Cuts at: 0 90 947 1133 1146 1282 1799 2040 2103
Size: 90 857 186 13 136 517 241 63
Fragments arranged by size:
857 517 241 186 136 90 63 13
EcoRI G'AATT_C
Cuts at: 0 492 2103
Size: 492 1611
HaeII r_GCGC'y
Cuts at: 0 1381 1701 1708 1899 2103
Size: 1381 320 7 191 204
Fragments arranged by size:
1381 320 204 191 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1324 1356 2103
Size: 1324 32 747
Fragments arranged by size:
1324 747 32
HindIII A'AGCT_T
Cuts at: 0 211 2103
Size: 211 1892
KasI G'GCGC_C
Cuts at: 0 1704 1895 2103
Size: 1704 191 208
Fragments arranged by size:
1704 208 191
KpnI G_GTAC'C
Cuts at: 0 1203 2103
Size: 1203 900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 1040 2103
Size: 473 567 1063
Fragments arranged by size:
1063 567 473
MscI TGG'CCA
Cuts at: 0 553 1299 2103
Size: 553 746 804
Fragments arranged by size:
804 746 553
MslI CAynn'nnrTG
Cuts at: 0 238 1357 1507 2103
Size: 238 1119 150 596
Fragments arranged by size:
1119 596 238 150
MspA1I CmG'CkG
Cuts at: 0 15 33 73 111 321 1329 1447 1663
Size: 15 18 40 38 210 1008 118 216
Cuts at: 1663 2103
Size: 440
Fragments arranged by size:
1008 440 216 210 118 40 38 18
15
NaeI GCC'GGC
Cuts at: 0 122 1906 2103
Size: 122 1784 197
Fragments arranged by size:
1784 197 122
NarI GG'CG_CC
Cuts at: 0 1705 1896 2103
Size: 1705 191 207
Fragments arranged by size:
1705 207 191
NcoI C'CATG_G
Cuts at: 0 548 2103
Size: 548 1555
NgoMIV G'CCGG_C
Cuts at: 0 120 1904 2103
Size: 120 1784 199
Fragments arranged by size:
1784 199 120
NspI r_CATG'y
Cuts at: 0 27 2103
Size: 27 2076
PasI CC'CwG_GG
Cuts at: 0 812 1795 2103
Size: 812 983 308
Fragments arranged by size:
983 812 308
PflMI CCAn_nnn'nTGG
Cuts at: 0 999 1362 2103
Size: 999 363 741
Fragments arranged by size:
999 741 363
PfoI T'CCnGG_A
Cuts at: 0 689 1457 2103
Size: 689 768 646
Fragments arranged by size:
768 689 646
PpuMI rG'GwC_Cy
Cuts at: 0 90 1282 1799 2040 2103
Size: 90 1192 517 241 63
Fragments arranged by size:
1192 517 241 90 63
PspOMI G'GGCC_C
Cuts at: 0 1133 2103
Size: 1133 970
PstI C_TGCA'G
Cuts at: 0 101 161 173 374 1145 2103
Size: 101 60 12 201 771 958
Fragments arranged by size:
958 771 201 101 60 12
PvuII CAG'CTG
Cuts at: 0 15 33 321 1329 1447 1663 2103
Size: 15 18 288 1008 118 216 440
Fragments arranged by size:
1008 440 288 216 118 18 15
SacI G_AGCT'C
Cuts at: 0 730 2103
Size: 730 1373
SanDI GG'GwC_CC
Cuts at: 0 1282 1799 2040 2103
Size: 1282 517 241 63
Fragments arranged by size:
1282 517 241 63
ScaI AGT'ACT
Cuts at: 0 625 2103
Size: 625 1478
SfcI C'TryA_G
Cuts at: 0 97 157 169 370 1141 2103
Size: 97 60 12 201 771 962
Fragments arranged by size:
962 771 201 97 60 12
SfoI GGC'GCC
Cuts at: 0 1706 1897 2103
Size: 1706 191 206
Fragments arranged by size:
1706 206 191
SmaI CCC'GGG
Cuts at: 0 527 2103
Size: 527 1576
SmlI C'TyrA_G
Cuts at: 0 463 967 1042 2103
Size: 463 504 75 1061
Fragments arranged by size:
1061 504 463 75
SphI G_CATG'C
Cuts at: 0 27 2103
Size: 27 2076
StuI AGG'CCT
Cuts at: 0 163 289 512 883 1436 2103
Size: 163 126 223 371 553 667
Fragments arranged by size:
667 553 371 223 163 126
StyI C'CwwG_G
Cuts at: 0 290 513 548 653 1571 1589 1634 2103
Size: 290 223 35 105 918 18 45 469
Fragments arranged by size:
918 469 290 223 105 45 35 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 73 817 1272 2103
Size: 73 744 455 831
Fragments arranged by size:
831 744 455 73
TatI w'GTAC_w
Cuts at: 0 623 2103
Size: 623 1480
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1518 1550 1632 1664 2103
Size: 1518 32 82 32 439
Fragments arranged by size:
1518 439 82 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1465 1602 2103
Size: 1465 137 501
Fragments arranged by size:
1465 501 137
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1359 2103
Size: 1359 744
XmaI C'CCGG_G
Cuts at: 0 525 2103
Size: 525 1578
ZraI GAC'GTC
Cuts at: 0 366 1822 2103
Size: 366 1456 281
Fragments arranged by size:
1456 366 281
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AleI AlwNI ApaI
ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI
BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SanDI ScaI SfcI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AlfI AloI
ApaLI AscI AseI AsiSI BaeI BamHI BcgI BciVI
BclI BfrBI BglII BmgBI BmtI BsaAI BsaWI BsiEI
BsiWI BsmI BspEI BsrGI BssHII BstBI BstYI BstZ17I
ClaI CspCI DraI DrdI EagI EciI EcoRV FalI
FseI FspI FspAI HincII HpaI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI XbaI XhoI XmnI