(Circular) (Six-base) MAPSORT of: pFN21AB5221.seq Check: 6200 from: 1 to: 5701 pFN21AB5221 5701 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2692 2692 Size: 5701 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3511 278 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3044 1937 Size: 1107 4594 Acc65I G'GTAC_C Cuts at: 3022 3022 Size: 5701 AclI AA'CG_TT Cuts at: 1563 3828 4201 1563 Size: 2265 373 3063 Fragments arranged by size: 3063 2265 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2657 3764 5148 5416 1632 Size: 1025 1107 1384 268 1917 Fragments arranged by size: 1917 1384 1107 1025 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5504 AflIII A'CryG_T Cuts at: 1203 4600 5577 1203 Size: 3397 977 1327 Fragments arranged by size: 3397 1327 977 AgeI A'CCGG_T Cuts at: 4532 4532 Size: 5701 AhdI GACnn_n'nnGTC Cuts at: 4430 4430 Size: 5701 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5701 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5701 AlwNI CAG_nnn'CTG Cuts at: 3444 5016 3444 Size: 1572 4129 ApaLI G'TGCA_C Cuts at: 2813 3758 4914 2813 Size: 945 1156 3600 Fragments arranged by size: 3600 1156 945 ApoI r'AATT_y Cuts at: 1493 2357 3010 3236 3388 5497 1493 Size: 864 653 226 152 2109 1697 Fragments arranged by size: 2109 1697 864 653 226 152 AseI AT'TA_AT Cuts at: 160 1032 4255 160 Size: 872 3223 1606 Fragments arranged by size: 3223 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 AvaI C'yCGr_G Cuts at: 1870 1943 3026 1870 Size: 73 1083 4545 Fragments arranged by size: 4545 1083 73 BamHI G'GATC_C Cuts at: 2979 3031 3398 2979 Size: 52 367 5282 Fragments arranged by size: 5282 367 52 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3022 4477 618 Size: 325 217 627 1235 1455 1842 Fragments arranged by size: 1842 1455 1235 627 325 217 BanII G_rGCy'C Cuts at: 1391 3020 1391 Size: 1629 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2175 2645 2857 928 Size: 971 276 470 212 3772 Fragments arranged by size: 3772 971 470 276 212 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3892 3926 1903 Size: 34 1955 34 3678 Fragments arranged by size: 3678 1955 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3593 4803 3593 Size: 1210 4491 BclI T'GATC_A Cuts at: 1548 2727 1548 Size: 1179 4522 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4312 136 Size: 107 122 71 631 605 2640 1525 Fragments arranged by size: 2640 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5696 5696 Size: 5701 BlpI GC'TnA_GC Cuts at: 3118 3118 Size: 5701 Bme1580I G_kGCm'C Cuts at: 1790 2299 2817 3762 4918 1790 Size: 509 518 945 1156 2573 Fragments arranged by size: 2573 1156 945 518 509 BmgBI CAC'GTC Cuts at: 1318 2300 1318 Size: 982 4719 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2813 3432 4390 Size: 416 53 83 399 24 1396 619 958 Cuts at: 4390 442 Size: 1753 Fragments arranged by size: 1753 1396 958 619 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5701 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4361 1239 Size: 3122 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3826 4691 4989 3826 Size: 865 298 4538 Fragments arranged by size: 4538 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4364 882 Size: 617 2865 2219 Fragments arranged by size: 2865 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2440 3492 493 Size: 711 1236 1052 2702 Fragments arranged by size: 2702 1236 1052 711 BsaBI GATnn'nnATC Cuts at: 5695 5695 Size: 5701 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3508 3890 4525 275 Size: 53 83 186 936 1975 382 635 1451 Fragments arranged by size: 1975 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4134 4532 4806 4953 5364 4134 Size: 398 274 147 411 4471 Fragments arranged by size: 4471 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5476 Fragments arranged by size: 5476 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5701 BseYI C'CCAG_C Cuts at: 2291 2536 4904 5400 2291 Size: 245 2368 496 2592 Fragments arranged by size: 2592 2368 496 245 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2630 2982 1163 Size: 573 894 352 3882 Fragments arranged by size: 3882 894 573 352 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3151 3912 4940 1540 Size: 275 186 1150 761 1028 2301 Fragments arranged by size: 2301 1150 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2817 3762 3847 4918 2817 Size: 945 85 1071 3600 Fragments arranged by size: 3600 1071 945 85 BsmI GAATG_Cn' Cuts at: 3212 3305 3212 Size: 93 5608 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2299 2817 3020 3762 3847 4918 1391 Size: 399 509 518 203 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 518 509 399 203 85 BspEI T'CCGG_A Cuts at: 5364 5364 Size: 5701 BspHI T'CATG_A Cuts at: 3590 5320 3590 Size: 1730 3971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2692 3056 844 Size: 1848 364 3489 Fragments arranged by size: 3489 1848 364 BsrBI CCG'CTC Cuts at: 1394 1823 3588 1394 Size: 429 1765 3507 Fragments arranged by size: 3507 1765 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4196 4370 65 Size: 1183 2948 174 1396 Fragments arranged by size: 2948 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4345 4532 1700 Size: 110 2535 187 2869 Fragments arranged by size: 2869 2535 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5701 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5701 BssSI C'ACGA_G Cuts at: 3761 4773 3761 Size: 1012 4689 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5701 BstBI TT'CG_AA Cuts at: 4505 4505 Size: 5701 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5701 BstYI r'GATC_y Cuts at: 1972 2194 2979 3031 3398 3783 3800 5252 5338 Size: 222 785 52 367 385 17 1452 86 Cuts at: 5338 5350 5696 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 785 385 367 346 222 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4210 Fragments arranged by size: 4210 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4962 Fragments arranged by size: 4962 683 56 BtsI GCAGTG_nn' Cuts at: 3217 4011 4031 3217 Size: 794 20 4887 Fragments arranged by size: 4887 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5701 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2403 2438 529 Size: 35 1839 35 3792 Fragments arranged by size: 3792 1839 35 35 DraI TTT'AAA Cuts at: 2356 3005 2356 Size: 649 5052 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4708 809 Size: 507 3392 1802 Fragments arranged by size: 3392 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3148 4037 5666 Size: 54 1425 50 275 18 1318 889 1629 Cuts at: 5666 8 Size: 43 Fragments arranged by size: 1629 1425 1318 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3148 1537 Size: 275 1336 4090 Fragments arranged by size: 4090 1336 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2934 2951 3631 1353 Size: 1581 17 680 3423 Fragments arranged by size: 3423 1581 680 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4288 4662 4808 387 Size: 1064 138 2699 374 146 1280 Fragments arranged by size: 2699 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2657 3764 4361 5148 5416 1239 Size: 393 1025 1107 597 787 268 1524 Fragments arranged by size: 1524 1107 1025 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2915 2915 Size: 5701 EcoO109I rG'GnC_Cy Cuts at: 2569 2569 Size: 5701 EcoRI G'AATT_C Cuts at: 3010 3388 3010 Size: 378 5323 EcoRV GAT'ATC Cuts at: 2428 2428 Size: 5701 FspI TGC'GCA Cuts at: 4207 4207 Size: 5701 HaeII r_GCGC'y Cuts at: 1678 4848 1678 Size: 3170 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2020 2052 2179 2211 2824 Size: 32 135 32 448 32 127 32 613 Cuts at: 2824 2856 4342 4374 4416 4448 1373 Size: 32 1486 32 42 32 2626 Fragments arranged by size: 2626 1486 613 448 135 127 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3045 3291 669 Size: 988 281 1107 246 3079 Fragments arranged by size: 3079 1107 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5701 HpaI GTT'AAC Cuts at: 3291 3291 Size: 5701 KpnI G_GTAC'C Cuts at: 3026 3026 Size: 5701 MfeI C'AATT_G Cuts at: 3300 3300 Size: 5701 MluI A'CGCG_T Cuts at: 5577 5577 Size: 5701 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2224 4815 4999 1305 Size: 919 2591 184 2007 Fragments arranged by size: 2591 2007 919 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3879 Fragments arranged by size: 3879 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3659 4018 4177 518 Size: 648 2493 359 159 2042 Fragments arranged by size: 2493 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 3117 3794 4942 5187 1606 Size: 420 1091 677 1148 245 2120 Fragments arranged by size: 2120 1148 1091 677 420 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4210 Fragments arranged by size: 4210 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5701 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5701 NotI GC'GGCC_GC Cuts at: 3148 3148 Size: 5701 NspI r_CATG'y Cuts at: 1132 1229 3059 4604 1132 Size: 97 1830 1545 2229 Fragments arranged by size: 2229 1830 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5701 PciI A'CATG_T Cuts at: 4600 4600 Size: 5701 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5701 PmeI GTTT'AAAC Cuts at: 3005 3005 Size: 5701 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3770 3802 5283 5315 3770 Size: 32 1481 32 4156 Fragments arranged by size: 4156 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2569 2569 Size: 5701 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5701 PsiI TTA'TAA Cuts at: 2034 3271 2034 Size: 1237 4464 PstI C_TGCA'G Cuts at: 830 3053 830 Size: 2223 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5701 PvuII CAG'CTG Cuts at: 2026 2026 Size: 5701 SalI G'TCGA_C Cuts at: 1936 3043 1936 Size: 1107 4594 SbfI CC_TGCA'GG Cuts at: 3053 3053 Size: 5701 ScaI AGT'ACT Cuts at: 3949 5661 3949 Size: 1712 3989 SfcI C'TryA_G Cuts at: 826 1045 2769 3049 4184 4865 5056 826 Size: 219 1724 280 1135 681 191 1471 Fragments arranged by size: 1724 1471 1135 681 280 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5701 SmaI CCC'GGG Cuts at: 1872 3028 1872 Size: 1156 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3805 4706 4968 820 Size: 197 926 1862 901 262 1553 Fragments arranged by size: 1862 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3492 493 Size: 2999 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5701 SphI G_CATG'C Cuts at: 1132 3059 1132 Size: 1927 3774 SspI AAT'ATT Cuts at: 5 52 3625 5 Size: 47 3573 2081 Fragments arranged by size: 3573 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5701 StyI C'CwwG_G Cuts at: 513 1065 2004 2572 3140 513 Size: 552 939 568 568 3074 Fragments arranged by size: 3074 939 568 568 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2553 2562 2664 2832 3728 3745 3898 Size: 1255 973 9 102 168 896 17 153 Cuts at: 3898 4083 4528 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 973 896 445 185 168 153 102 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3947 5659 96 Size: 274 80 33 51 1443 1970 1712 138 Fragments arranged by size: 1970 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2440 2472 2807 2839 5661 5693 2440 Size: 32 335 32 2822 32 2448 Fragments arranged by size: 2822 2448 335 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5701 XbaI T'CTAG_A Cuts at: 3037 3037 Size: 5701 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5701 XmaI C'CCGG_G Cuts at: 1870 3026 1870 Size: 1156 4545 XmnI GAAnn'nnTTC Cuts at: 3830 3830 Size: 5701 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3509 276 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI