Restriction map for
pFN21AB5018
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspHI AviII AvrII BalI
BamHI BbrPI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I CelII
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NciI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I TspRI XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 393 GACGTC GACGT^C
Acc65I 250 GGTACC G^GTACC
AcsI 602 GAATTC [GA]^AATT[CT]
AfaI 251 GTAC GT^AC
AlwI 48 GATCC GGATC(4/5)
AocI 323 CCTCAGG CC^T[ACGT]AGG
ApoI 602 GAATTC [GA]^AATT[CT]
Asp718I 250 GGTACC G^GTACC
AspI 169 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 616 CCCGAG C^[CT]CG[GA]G
AvaII 705 GGACC G^G[AT]CC
BbeI 901 GGCGCC GGCGC^C
BbsI 451 GAAGAC GAAGAC(2/6)
BfaI 469 CTAG C^TAG
BglII 158 AGATCT A^GATCT
BpuAI 451 GAAGAC GAAGAC(2/6)
BseRI 913 GAGGAG GAGGAG(10/8)
BsoBI 616 CCCGAG C^[CT]CG[GA]G
BspDI 196 ATCGAT AT^CGAT
BsrBI 532 GAGCGG CCGCTC(-3/-3)
BsrI 714 CCAGT ACTGG(1/-1)
BstEII 225 GGTGACC G^GT[ACGT]ACC
BstPI 225 GGTGACC G^GT[ACGT]ACC
Bsu36I 323 CCTCAGG CC^T[ACGT]AGG
ClaI 196 ATCGAT AT^CGAT
EarI 426 CTCTTC CTCTTC(1/4)
Eco81I 323 CCTCAGG CC^T[ACGT]AGG
EcoO65I 225 GGTGACC G^GT[ACGT]ACC
EcoRI 602 GAATTC G^AATTC
EcoT14I 515 CCATGG C^C[AT][AT]GG
HaeII 901 GGCGCC [GA]GCGC^[CT]
KasI 901 GGCGCC G^GCGCC
KpnI 250 GGTACC GGTAC^C
Ksp632I 426 CTCTTC CTCTTC(1/4)
MaeI 469 CTAG C^TAG
MaeII 394 ACGT A^CGT
NarI 901 GGCGCC GG^CGCC
NcoI 515 CCATGG C^CATGG
PflFI 169 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 774 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 28 CTGCAG CTGCA^G
RsaI 251 GTAC GT^AC
SfoI 901 GGCGCC GGC^GCC
SmlI 162 CTTGAG C^T[CT][GA]AG
StyI 515 CCATGG C^C[AT][AT]GG
TaqI 197 TCGA T^CGA
Tsp509I 603 AATT ^AATT
Tth111I 169 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 197 TCGA T^CGA
Van91I 774 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 456 CGCG CG^CG
1023 CGCG
BbvCI 254 CCTCAGC CCTCAGC(-5/-2)
796 CCTCAGC
BpmI 772 CTCCAG CTGGAG(16/14)
691 CTGGAG
BsmAI 67 GAGAC GTCTC(1/5)
490 GAGAC
BsmBI 67 GAGACG CGTCTC(1/5)
490 GAGACG
BspMI 345 ACCTGC ACCTGC(4/8)
732 GCAGGT
BstUI 456 CGCG CG^CG
1023 CGCG
BstYI 47 AGATCC [GA]^GATC[CT]
158 AGATCT
CfoI 754 GCGC GCG^C
902 GCGC
DraII 917 AGGCCCC [GA]G^G[ACGT]CC[CT]
980 AGGCCCT
EcoO109I 917 AGGCCCC [GA]G^G[ACGT]CC[CT]
980 AGGCCCT
HgaI 454 GACGC GACGC(5/10)
945 GACGC
HhaI 754 GCGC GCG^C
902 GCGC
HinP1I 754 GCGC G^CGC
902 GCGC
MflI 47 AGATCC [GA]^GATC[CT]
158 AGATCT
MvnI 456 CGCG CG^CG
1023 CGCG
NspI 329 GCATGT [GA]CATG^[CT]
725 ACATGC
PvuII 281 CAGCTG CAG^CTG
778 CAGCTG
SfcI 28 CTGCAG C^T[GA][CT]AG
965 CTATAG
StuI 269 AGGCCT AGG^CCT
1013 AGGCCT
TfiI 379 GAATC G^A[AT]TC
738 GATTC
XhoII 47 AGATCC [GA]^GATC[CT]
158 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcyI AluI AlwNI BanI
BanII BbvI BmyI BsaHI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrFI
BssKI BstF5I BstNI Cac8I Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII Hin1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NaeI NdeII NgoMIV NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I