(Circular) (Six-base) MAPSORT of: pFN21AB4990.seq Check: 206 from: 1 to: 6184 pFN21AB4990 6184 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3994 278 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3527 1937 Size: 1590 4594 Acc65I G'GTAC_C Cuts at: 3505 3505 Size: 6184 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3266 4247 5631 5899 1632 Size: 1634 981 1384 268 1917 Fragments arranged by size: 1917 1634 1384 981 268 AfeI AGC'GCT Cuts at: 2034 2034 Size: 6184 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5987 AflIII A'CryG_T Cuts at: 1203 5083 6060 1203 Size: 3880 977 1327 Fragments arranged by size: 3880 1327 977 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 4913 4913 Size: 6184 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2957 1184 Size: 1773 4411 AlwNI CAG_nnn'CTG Cuts at: 2266 3479 3927 5499 2266 Size: 1213 448 1572 2951 Fragments arranged by size: 2951 1572 1213 448 ApaI G_GGCC'C Cuts at: 2023 2860 2023 Size: 837 5347 ApaLI G'TGCA_C Cuts at: 4241 5397 4241 Size: 1156 5028 ApoI r'AATT_y Cuts at: 1493 3493 3719 3871 5980 1493 Size: 2000 226 152 2109 1697 Fragments arranged by size: 2109 2000 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4738 160 Size: 872 3706 1606 Fragments arranged by size: 3706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 2400 3509 1870 Size: 73 457 1109 4545 Fragments arranged by size: 4545 1109 457 73 BamHI G'GATC_C Cuts at: 2404 2668 3514 3881 2404 Size: 264 846 367 4707 Fragments arranged by size: 4707 846 367 264 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2887 3026 3505 4960 618 Size: 325 217 627 1100 139 479 1455 1842 Fragments arranged by size: 1842 1455 1100 627 479 325 217 139 BanII G_rGCy'C Cuts at: 1391 2023 2244 2397 2769 2860 2951 3503 1391 Size: 632 221 153 372 91 91 552 4072 Fragments arranged by size: 4072 632 552 372 221 153 91 91 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3254 928 Size: 971 1355 3858 Fragments arranged by size: 3858 1355 971 BbvCI CC'TCA_GC Cuts at: 2359 2364 2912 2359 Size: 5 548 5631 Fragments arranged by size: 5631 548 5 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3195 3229 4375 4409 1903 Size: 34 1258 34 1146 34 3678 Fragments arranged by size: 3678 1258 1146 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4076 5286 4076 Size: 1210 4974 BclI T'GATC_A Cuts at: 1548 1548 Size: 6184 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3134 4795 136 Size: 107 122 71 631 605 1462 1661 1525 Fragments arranged by size: 1661 1525 1462 631 605 122 107 71 BglII A'GATC_T Cuts at: 2553 6179 2553 Size: 3626 2558 BlpI GC'TnA_GC Cuts at: 2945 3601 2945 Size: 656 5528 Bme1580I G_kGCm'C Cuts at: 1790 2023 2860 2892 4245 5401 1790 Size: 233 837 32 1353 1156 2573 Fragments arranged by size: 2573 1353 1156 837 233 32 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6184 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3915 4873 442 Size: 416 53 83 399 24 2498 958 1753 Fragments arranged by size: 2498 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3051 1056 Size: 1995 4189 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2150 2162 2411 2440 2761 2999 3230 Size: 878 33 12 249 29 321 238 231 Cuts at: 3230 4844 1239 Size: 1614 2579 Fragments arranged by size: 2579 1614 878 321 249 238 231 33 29 12 Bpu10I CC'TnA_GC Cuts at: 2359 2364 2912 2359 Size: 5 548 5631 Fragments arranged by size: 5631 548 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4309 5174 5472 4309 Size: 865 298 5021 Fragments arranged by size: 5021 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4847 882 Size: 617 3348 2219 Fragments arranged by size: 3348 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3975 493 Size: 711 2771 2702 Fragments arranged by size: 2771 2702 711 BsaBI GATnn'nnATC Cuts at: 6178 6178 Size: 6184 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3114 3449 3991 4373 Size: 53 83 186 936 1581 335 542 382 Cuts at: 4373 5008 275 Size: 635 1451 Fragments arranged by size: 1581 1451 936 635 542 382 335 186 83 53 BsaWI w'CCGG_w Cuts at: 2158 2307 4617 5015 5289 5436 5847 2158 Size: 149 2310 398 274 147 411 2495 Fragments arranged by size: 2495 2310 411 398 274 149 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5959 Fragments arranged by size: 5959 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2531 1186 Size: 1345 4839 BseYI C'CCAG_C Cuts at: 2769 2950 5387 5883 2769 Size: 181 2437 496 3070 Fragments arranged by size: 3070 2437 496 181 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2992 3189 3326 1163 Size: 573 1256 197 137 4021 Fragments arranged by size: 4021 1256 573 197 137 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3634 4395 5423 1540 Size: 275 186 1633 761 1028 2301 Fragments arranged by size: 2301 1633 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2244 2397 4245 4330 5401 2244 Size: 153 1848 85 1071 3027 Fragments arranged by size: 3027 1848 1071 153 85 BsmI GAATG_Cn' Cuts at: 2466 3695 3788 2466 Size: 1229 93 4862 Fragments arranged by size: 4862 1229 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3125 3125 Size: 6184 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2244 2397 2769 2860 2892 2951 Size: 399 233 221 153 372 91 32 59 Cuts at: 2951 3503 4245 4330 5401 1391 Size: 552 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 552 399 372 233 221 153 91 85 59 32 BspEI T'CCGG_A Cuts at: 2158 2307 5847 2158 Size: 149 3540 2495 Fragments arranged by size: 3540 2495 149 BspHI T'CATG_A Cuts at: 2661 4073 5803 2661 Size: 1412 1730 3042 Fragments arranged by size: 3042 1730 1412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2172 3168 3180 3539 844 Size: 1328 996 12 359 3489 Fragments arranged by size: 3489 1328 996 359 12 BsrBI CCG'CTC Cuts at: 1394 1823 2788 3289 4071 1394 Size: 429 965 501 782 3507 Fragments arranged by size: 3507 965 782 501 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3057 4679 4853 65 Size: 1183 1809 1622 174 1396 Fragments arranged by size: 1809 1622 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3429 4828 5015 1700 Size: 110 1619 1399 187 2869 Fragments arranged by size: 2869 1619 1399 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6184 BssSI C'ACGA_G Cuts at: 4244 5256 4244 Size: 1012 5172 BstAPI GCAn_nnn'nTGC Cuts at: 794 2869 794 Size: 2075 4109 BstBI TT'CG_AA Cuts at: 4988 4988 Size: 6184 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6184 BstXI CCAn_nnnn'nTGG Cuts at: 2095 2692 3064 2095 Size: 597 372 5215 Fragments arranged by size: 5215 597 372 BstYI r'GATC_y Cuts at: 1972 2073 2404 2553 2668 3309 3514 3881 4266 Size: 101 331 149 115 641 205 367 385 Cuts at: 4266 4283 5735 5821 5833 6179 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 641 385 367 346 331 205 149 115 101 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2524 3084 3321 2524 Size: 560 237 5387 Fragments arranged by size: 5387 560 237 BtgI C'CryG_G Cuts at: 513 1065 2004 2832 513 Size: 552 939 828 3865 Fragments arranged by size: 3865 939 828 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5445 Fragments arranged by size: 5445 683 56 BtsI GCAGTG_nn' Cuts at: 2766 2961 3042 3453 3700 4494 4514 2766 Size: 195 81 411 247 794 20 4436 Fragments arranged by size: 4436 794 411 247 195 81 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2908 2943 529 Size: 35 2344 35 3770 Fragments arranged by size: 3770 2344 35 35 DraI TTT'AAA Cuts at: 3488 3488 Size: 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5191 809 Size: 507 3875 1802 Fragments arranged by size: 3875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3631 4520 6149 Size: 54 1425 50 275 18 1801 889 1629 Cuts at: 6149 8 Size: 43 Fragments arranged by size: 1801 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3631 1537 Size: 275 1819 4090 Fragments arranged by size: 4090 1819 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2030 2778 4114 1353 Size: 665 12 748 1336 3423 Fragments arranged by size: 3423 1336 748 665 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4771 5145 5291 387 Size: 1064 138 3182 374 146 1280 Fragments arranged by size: 3182 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2242 2395 2242 Size: 153 6031 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2150 2162 2411 2440 2761 2999 Size: 393 485 33 12 249 29 321 238 Cuts at: 2999 3230 3266 4247 4844 5631 5899 1239 Size: 231 36 981 597 787 268 1524 Fragments arranged by size: 1524 981 787 597 485 393 321 268 249 238 231 36 33 29 12 EcoNI CCTnn'n_nnAGG Cuts at: 2663 2663 Size: 6184 EcoO109I rG'GnC_Cy Cuts at: 2019 2020 2712 2856 2857 2989 3151 3238 2019 Size: 1 692 144 1 132 162 87 4965 Fragments arranged by size: 4965 692 162 144 132 87 1 1 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 FspI TGC'GCA Cuts at: 2621 3158 3176 4690 2621 Size: 537 18 1514 4115 Fragments arranged by size: 4115 1514 537 18 HaeII r_GCGC'y Cuts at: 1678 2036 2096 3129 5331 1678 Size: 358 60 1033 2202 2531 Fragments arranged by size: 2531 2202 1033 358 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2304 2336 2357 2389 2492 Size: 32 135 32 732 32 21 32 103 Cuts at: 2492 2524 4825 4857 4899 4931 1373 Size: 32 2301 32 42 32 2626 Fragments arranged by size: 2626 2301 732 135 103 42 32 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 669 1657 1938 3528 3774 669 Size: 988 281 1590 246 3079 Fragments arranged by size: 3079 1590 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6184 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KpnI G_GTAC'C Cuts at: 3509 3509 Size: 6184 MfeI C'AATT_G Cuts at: 3783 3783 Size: 6184 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3219 5298 5482 1305 Size: 1914 2079 184 2007 Fragments arranged by size: 2079 2007 1914 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4362 Fragments arranged by size: 4362 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3107 4142 4501 4660 518 Size: 648 1941 1035 359 159 2042 Fragments arranged by size: 2042 1941 1035 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2317 2363 2411 2773 2866 3094 3166 3479 Size: 711 46 48 362 93 228 72 313 Cuts at: 3479 3600 4277 5425 5670 1606 Size: 121 677 1148 245 2120 Fragments arranged by size: 2120 1148 711 677 362 313 245 228 121 93 72 48 46 NcoI C'CATG_G Cuts at: 513 1065 2004 2832 513 Size: 552 939 828 3865 Fragments arranged by size: 3865 939 828 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6184 NheI G'CTAG_C Cuts at: 1052 3047 1052 Size: 1995 4189 NotI GC'GGCC_GC Cuts at: 3631 3631 Size: 6184 NspI r_CATG'y Cuts at: 1132 1229 2054 2908 3542 5087 1132 Size: 97 825 854 634 1545 2229 Fragments arranged by size: 2229 1545 854 825 634 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6184 PciI A'CATG_T Cuts at: 5083 5083 Size: 6184 PflMI CCAn_nnn'nTGG Cuts at: 1108 2899 1108 Size: 1791 4393 PfoI T'CCnGG_A Cuts at: 2601 2601 Size: 6184 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4253 4285 5766 5798 4253 Size: 32 1481 32 4639 Fragments arranged by size: 4639 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2989 2989 Size: 6184 PshAI GACnn'nnGTC Cuts at: 1613 3117 1613 Size: 1504 4680 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PspOMI G'GGCC_C Cuts at: 2019 2856 2019 Size: 837 5347 PstI C_TGCA'G Cuts at: 830 2778 2865 3261 3536 830 Size: 1948 87 396 275 3478 Fragments arranged by size: 3478 1948 396 275 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 PvuII CAG'CTG Cuts at: 2317 2363 2773 2866 3094 3166 3479 2317 Size: 46 410 93 228 72 313 5022 Fragments arranged by size: 5022 410 313 228 93 72 46 SacI G_AGCT'C Cuts at: 2244 2397 2244 Size: 153 6031 SalI G'TCGA_C Cuts at: 1936 3526 1936 Size: 1590 4594 SapI GCTCTTCn'nnn_ Cuts at: 2778 2778 Size: 6184 SbfI CC_TGCA'GG Cuts at: 3536 3536 Size: 6184 ScaI AGT'ACT Cuts at: 4432 6144 4432 Size: 1712 4472 SfcI C'TryA_G Cuts at: 826 1045 2774 2861 3257 3532 4667 5348 5539 Size: 219 1729 87 396 275 1135 681 191 Cuts at: 5539 826 Size: 1471 Fragments arranged by size: 1729 1471 1135 681 396 275 219 191 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6184 SmaI CCC'GGG Cuts at: 1872 2402 3511 1872 Size: 530 1109 4545 Fragments arranged by size: 4545 1109 530 SmlI C'TyrA_G Cuts at: 820 1017 1943 4288 5189 5451 820 Size: 197 926 2345 901 262 1553 Fragments arranged by size: 2345 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3975 493 Size: 3482 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 3542 1132 Size: 2410 3774 SspI AAT'ATT Cuts at: 5 52 4108 5 Size: 47 4056 2081 Fragments arranged by size: 4056 2081 47 StuI AGG'CCT Cuts at: 1517 2447 1517 Size: 930 5254 StyI C'CwwG_G Cuts at: 513 1065 2004 2088 2292 2832 3623 513 Size: 552 939 84 204 540 791 3074 Fragments arranged by size: 3074 939 791 552 540 204 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4211 4228 4381 4566 5011 325 Size: 1255 2631 17 153 185 445 1498 Fragments arranged by size: 2631 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4430 6142 96 Size: 274 80 33 51 1443 2453 1712 138 Fragments arranged by size: 2453 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3283 3315 6144 6176 3283 Size: 32 2829 32 3291 Fragments arranged by size: 3291 2829 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6184 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2290 2689 2758 3106 2290 Size: 399 69 348 5368 Fragments arranged by size: 5368 399 348 69 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6184 XmaI C'CCGG_G Cuts at: 1870 2400 3509 1870 Size: 530 1109 4545 Fragments arranged by size: 4545 1109 530 XmnI GAAnn'nnTTC Cuts at: 3190 4313 3190 Size: 1123 5061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3992 276 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI