Restriction map for
pFN21AB4976
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HindIII HpaI KasI
KspI MfeI MluI MseI MspA1I
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 1225 GGTACC G^GTACC
AccIII 503 TCCGGA T^CCGGA
AcyI 1107 GACGCC G[GA]^CG[CT]C
AlwNI 1180 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 394 GGGCCC GGGCC^C
Asp718I 1225 GGTACC G^GTACC
AspHI 328 GAGCAC G[AT]GC[AT]^C
BalI 242 TGGCCA TGG^CCA
BamHI 129 GGATCC G^GATCC
BanII 394 GGGCCC G[GA]GC[CT]^C
BcgI 787 GCACCCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 1107 GACGCC G[GA]^CG[CT]C
BsaI 553 GAGACC GGTCTC(1/5)
BsaWI 503 TCCGGA [AT]^CCGG[AT]
BseAI 503 TCCGGA T^CCGGA
BsiHKAI 328 GAGCAC G[AT]GC[AT]^C
BsmBI 1105 GAGACG CGTCTC(1/5)
BspEI 503 TCCGGA T^CCGGA
BspMI 172 ACCTGC ACCTGC(4/8)
BsrBI 148 GAGCGG CCGCTC(-3/-3)
BssSI 97 CTCGTG CACGAG(-5/-1)
BstXI 1019 CCAGCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRI 483 GAATTC G^AATTC
Hin1I 1107 GACGCC G[GA]^CG[CT]C
HincII 646 GTCAAC GT[CT]^[GA]AC
HindII 646 GTCAAC GT[CT]^[GA]AC
HinfI 155 GAGTC G^A[ACGT]TC
KpnI 1225 GGTACC GGTAC^C
MluNI 242 TGGCCA TGG^CCA
MroI 503 TCCGGA T^CCGGA
MscI 242 TGGCCA TGG^CCA
MslI 1047 CACCCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 155 GAGTC GAGTC(4/5)
SmlI 877 CTCAAG C^T[CT][GA]AG
StuI 529 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 58 GTCTAC GT^[AC][GT]AC
811 GTCTAC
AccII 293 CGCG CG^CG
450 CGCG
AcsI 456 GAATTT [GA]^AATT[CT]
483 GAATTC
ApoI 456 GAATTT [GA]^AATT[CT]
483 GAATTC
BbsI 400 GAAGAC GAAGAC(2/6)
886 GAAGAC
BbvI 387 GCAGC GCAGC(8/12)
297 GCTGC
BcnI 192 CCGGG CC^[GC]GG
391 CCGGG
BpuAI 400 GAAGAC GAAGAC(2/6)
886 GAAGAC
BsaBI 690 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1117 GATACCAATC
BseRI 463 GAGGAG GAGGAG(10/8)
1216 GAGGAG
BsmFI 13 GGGAC GGGAC(10/14)
74 GGGAC
BsrFI 264 ACCGGC [GA]^CCGG[CT]
1228 ACCGGC
BstUI 293 CGCG CG^CG
450 CGCG
Cfr10I 264 ACCGGC [GA]^CCGG[CT]
1228 ACCGGC
DrdI 199 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
427 GACTATGAAGTC
EarI 766 GAAGAG CTCTTC(1/4)
1213 GAAGAG
Ksp632I 766 GAAGAG CTCTTC(1/4)
1213 GAAGAG
MamI 690 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1117 GATACCAATC
MvnI 293 CGCG CG^CG
450 CGCG
NciI 192 CCGGG CC^[GC]GG
391 CCGGG
NcoI 627 CCATGG C^CATGG
761 CCATGG
PstI 298 CTGCAG CTGCA^G
385 CTGCAG
SfaNI 1015 GCATC GCATC(5/9)
1083 GCATC
SfcI 298 CTGCAG C^T[GA][CT]AG
385 CTGCAG
TaqI 92 TCGA T^CGA
796 TCGA
TseI 297 GCTGC G^C[AT]GC
387 GCAGC
Tsp509I 457 AATT ^AATT
484 AATT
TthHB8I 92 TCGA T^CGA
796 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanI BfaI BglII BmyI
BsaJI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV PpuMI RsaI Sau3AI Sau96I
ScrFI StyI Tsp45I TspRI XhoII