Restriction map for
pFN21AB4862
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1432 GACGTC GACGT^C
AccI 409 GTAGAC GT^[AC][GT]AC
AcyI 1432 GACGTC G[GA]^CG[CT]C
AlwNI 1387 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1408 CCTGAGG CC^T[ACGT]AGG
Asp700I 387 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 715 GAGCTC G[AT]GC[AT]^C
BalI 1244 TGGCCA TGG^CCA
BamHI 323 GGATCC G^GATCC
BanI 1036 GGCACC G^G[CT][GA]CC
BcnI 338 CCCGG CC^[GC]GG
BsaAI 1360 TACGTG [CT]AC^GT[GA]
BsaHI 1432 GACGTC G[GA]^CG[CT]C
BsaI 764 GAGACC GGTCTC(1/5)
BsiHKAI 715 GAGCTC G[AT]GC[AT]^C
BspLU11I 836 ACATGT A^CATGT
BspMI 1382 ACCTGC ACCTGC(4/8)
Bsu36I 1408 CCTGAGG CC^T[ACGT]AGG
DraII 75 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1081 GACAAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1302 CCGTGG C^C[GA][CT]GG
EaeI 1244 TGGCCA [CT]^GGCC[GA]
EarI 712 GAAGAG CTCTTC(1/4)
Eco81I 1408 CCTGAGG CC^T[ACGT]AGG
EcoNI 1071 CCTCAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 75 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1031 CCAAGG C^C[AT][AT]GG
HaeII 48 AGCGCC [GA]GCGC^[CT]
HgaI 690 GACGC GACGC(5/10)
Hin1I 1432 GACGTC G[GA]^CG[CT]C
HincII 565 GTCAAC GT[CT]^[GA]AC
HindII 565 GTCAAC GT[CT]^[GA]AC
Ksp632I 712 GAAGAG CTCTTC(1/4)
MluNI 1244 TGGCCA TGG^CCA
MscI 1244 TGGCCA TGG^CCA
MslI 837 CATGTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 338 CCCGG CC^[GC]GG
PaeR7I 472 CTCGAG C^TCGAG
PflMI 1247 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 75 AGGACCT [GA]G^G[AT]CC[CT]
SacI 715 GAGCTC GAGCT^C
SapI 712 GAAGAGC GCTCTTC(1/4)
SfaNI 1444 GATGC GCATC(5/9)
SphI 627 GCATGC GCATG^C
StyI 1031 CCAAGG C^C[AT][AT]GG
Van91I 1247 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 516 TCTAGA T^CTAGA
XcmI 1132 CCAATCCAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 472 CTCGAG C^TCGAG
XmnI 387 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 391 GAATTC [GA]^AATT[CT]
1216 GAATTC
AfaI 1365 GTAC GT^AC
1401 GTAC
AflIII 836 ACATGT A^C[GA][CT]GT
1361 ACGTGT
ApoI 391 GAATTC [GA]^AATT[CT]
1216 GAATTC
AvaI 472 CTCGAG C^[CT]CG[GA]G
868 CTCGGG
BanII 661 GAGCCC G[GA]GC[CT]^C
715 GAGCTC
BpmI 772 CTCCAG CTGGAG(16/14)
933 CTGGAG
BseRI 718 CTCCTC GAGGAG(10/8)
1411 GAGGAG
BsgI 1417 CTGCAC GTGCAG(16/14)
224 GTGCAG
BsoBI 472 CTCGAG C^[CT]CG[GA]G
868 CTCGGG
BstAPI 1386 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1419 GCACGCCATGC
CfoI 49 GCGC GCG^C
449 GCGC
EcoRI 391 GAATTC G^AATTC
1216 GAATTC
HapII 339 CCGG C^CGG
1006 CCGG
HhaI 49 GCGC GCG^C
449 GCGC
HinP1I 49 GCGC G^CGC
449 GCGC
HpaII 339 CCGG C^CGG
1006 CCGG
MaeII 1361 ACGT A^CGT
1433 ACGT
MaeIII 823 GTCAC ^GT[ACGT]AC
1379 GTGAC
MspI 339 CCGG C^CGG
1006 CCGG
NspI 627 GCATGC [GA]CATG^[CT]
836 ACATGT
PvuII 1138 CAGCTG CAG^CTG
1194 CAGCTG
RsaI 1365 GTAC GT^AC
1401 GTAC
TfiI 16 GATTC G^A[AT]TC
583 GAATC
Tsp45I 823 GTCAC ^GT[GC]AC
1379 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MboI MboII MflI MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PstI Sau3AI
Sau96I ScrFI SfcI SmlI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII