Restriction map for
pFN21AB4862
(Linear) (Six-base) MAPSORT of: KIBB4862.seq Check: 3992 from: 1 to: 1455
KIBB4862.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AatII G_ACGT'C
Cuts at: 0 1436 1455
Size: 1436 19
AccI GT'mk_AC
Cuts at: 0 410 1455
Size: 410 1045
AflIII A'CryG_T
Cuts at: 0 836 1361 1455
Size: 836 525 94
Fragments arranged by size:
836 525 94
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 462 1455
Size: 462 993
AlwNI CAG_nnn'CTG
Cuts at: 0 1392 1455
Size: 1392 63
ApoI r'AATT_y
Cuts at: 0 391 1216 1455
Size: 391 825 239
Fragments arranged by size:
825 391 239
AvaI C'yCGr_G
Cuts at: 0 472 868 1455
Size: 472 396 587
Fragments arranged by size:
587 472 396
BamHI G'GATC_C
Cuts at: 0 323 1455
Size: 323 1132
BanI G'GyrC_C
Cuts at: 0 1036 1455
Size: 1036 419
BanII G_rGCy'C
Cuts at: 0 665 719 1455
Size: 665 54 736
Fragments arranged by size:
736 665 54
Bme1580I G_kGCm'C
Cuts at: 0 1039 1455
Size: 1039 416
BmrI ACTGGGnnnn_n'
Cuts at: 0 328 378 627 921 1455
Size: 328 50 249 294 534
Fragments arranged by size:
534 328 294 249 50
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 460 738 787 1455
Size: 460 278 49 668
Fragments arranged by size:
668 460 278 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 954 1455
Size: 757 197 501
Fragments arranged by size:
757 501 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 712 1455
Size: 254 458 743
Fragments arranged by size:
743 458 254
BsaI GGTCTCn'nnnn_
Cuts at: 0 758 1455
Size: 758 697
BsaAI yAC'GTr
Cuts at: 0 1362 1455
Size: 1362 93
BsaHI Gr'CG_yC
Cuts at: 0 1433 1455
Size: 1433 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 840 870 1455
Size: 840 30 585
Fragments arranged by size:
840 585 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 709 1426 1455
Size: 709 717 29
Fragments arranged by size:
717 709 29
BseYI C'CCAG_C
Cuts at: 0 22 570 664 1211 1395 1455
Size: 22 548 94 547 184 60
Fragments arranged by size:
548 547 184 94 60 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 1402 1455
Size: 245 1157 53
Fragments arranged by size:
1157 245 53
BsiHKAI G_wGCw'C
Cuts at: 0 719 1455
Size: 719 736
Bsp1286I G_dGCh'C
Cuts at: 0 665 719 1039 1455
Size: 665 54 320 416
Fragments arranged by size:
665 416 320 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1391 1455
Size: 1391 64
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1392 1425 1455
Size: 1392 33 30
Fragments arranged by size:
1392 33 30
BstYI r'GATC_y
Cuts at: 0 96 323 521 1455
Size: 96 227 198 934
Fragments arranged by size:
934 227 198 96
Bsu36I CC'TnA_GG
Cuts at: 0 1409 1455
Size: 1409 46
BtgI C'CryG_G
Cuts at: 0 1302 1455
Size: 1302 153
BtsI GCAGTG_nn'
Cuts at: 0 68 349 1131 1455
Size: 68 281 782 324
Fragments arranged by size:
782 324 281 68
DrdI GACnn_nn'nnGTC
Cuts at: 0 1087 1455
Size: 1087 368
EaeI y'GGCC_r
Cuts at: 0 1244 1455
Size: 1244 211
EarI CTCTTCn'nnn_
Cuts at: 0 707 1455
Size: 707 748
EcoICRI GAG'CTC
Cuts at: 0 717 1455
Size: 717 738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 954 1455
Size: 757 197 501
Fragments arranged by size:
757 501 197
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1075 1455
Size: 1075 380
EcoO109I rG'GnC_Cy
Cuts at: 0 76 1455
Size: 76 1379
EcoRI G'AATT_C
Cuts at: 0 391 1216 1455
Size: 391 825 239
Fragments arranged by size:
825 391 239
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1056 1455
Size: 1056 399
HaeII r_GCGC'y
Cuts at: 0 52 1455
Size: 52 1403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 191 308 340 681 713 733 765
Size: 159 32 117 32 341 32 20 32
Cuts at: 765 840 872 1455
Size: 75 32 583
Fragments arranged by size:
583 341 159 117 75 32 32 32
32 32 20
HincII GTy'rAC
Cuts at: 0 567 1455
Size: 567 888
MscI TGG'CCA
Cuts at: 0 1246 1455
Size: 1246 209
MslI CAynn'nnrTG
Cuts at: 0 841 1455
Size: 841 614
MspA1I CmG'CkG
Cuts at: 0 1140 1196 1348 1455
Size: 1140 56 152 107
Fragments arranged by size:
1140 152 107 56
NspI r_CATG'y
Cuts at: 0 631 840 1455
Size: 631 209 615
Fragments arranged by size:
631 615 209
PasI CC'CwG_GG
Cuts at: 0 453 1455
Size: 453 1002
PciI A'CATG_T
Cuts at: 0 836 1455
Size: 836 619
PflMI CCAn_nnn'nTGG
Cuts at: 0 1253 1455
Size: 1253 202
PfoI T'CCnGG_A
Cuts at: 0 931 1455
Size: 931 524
PpuMI rG'GwC_Cy
Cuts at: 0 76 1455
Size: 76 1379
PstI C_TGCA'G
Cuts at: 0 63 530 965 1388 1455
Size: 63 467 435 423 67
Fragments arranged by size:
467 435 423 67 63
PvuII CAG'CTG
Cuts at: 0 1140 1196 1455
Size: 1140 56 259
Fragments arranged by size:
1140 259 56
SacI G_AGCT'C
Cuts at: 0 719 1455
Size: 719 736
SapI GCTCTTCn'nnn_
Cuts at: 0 707 1455
Size: 707 748
SfcI C'TryA_G
Cuts at: 0 59 526 961 1384 1455
Size: 59 467 435 423 71
Fragments arranged by size:
467 435 423 71 59
SmlI C'TyrA_G
Cuts at: 0 269 472 727 1455
Size: 269 203 255 728
Fragments arranged by size:
728 269 255 203
SphI G_CATG'C
Cuts at: 0 631 1455
Size: 631 824
StyI C'CwwG_G
Cuts at: 0 1031 1455
Size: 1031 424
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 360 741 940 1455
Size: 360 381 199 515
Fragments arranged by size:
515 381 360 199
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 843 875 1455
Size: 843 32 580
Fragments arranged by size:
843 580 32
XbaI T'CTAG_A
Cuts at: 0 516 1455
Size: 516 939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1139 1455
Size: 1139 316
XhoI C'TCGA_G
Cuts at: 0 472 1455
Size: 472 983
XmnI GAAnn'nnTTC
Cuts at: 0 391 1455
Size: 391 1064
ZraI GAC'GTC
Cuts at: 0 1434 1455
Size: 1434 21
Enzymes that do cut:
AatII AccI AflIII AlfI AlwNI ApoI AvaI BamHI
BanI BanII Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BstAPI BstYI Bsu36I BtgI BtsI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII MscI MslI MspA1I NspI PasI PciI
PflMI PfoI PpuMI PstI PvuII SacI SapI SfcI
SmlI SphI StyI TaqII TstI XbaI XcmI XhoI
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI Bpu10I BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII EagI EciI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI
Tth111I XmaI