Restriction map for
pFN21AB4600
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BanI BanII BbeI
BbsI BciVI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tsp45I
TspRI Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AclI 10 AACGTT AA^CGTT
AflIII 423 ACGTGT A^C[GA][CT]GT
Asp700I 488 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 792 GGACC G^G[AT]CC
BbrPI 422 CACGTG CAC^GTG
BcgI 103 GCAATCTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 845 TGATCA T^GATCA
BmyI 460 GTGCCC G[AGT]GC[ACT]^C
BpmI 838 CTGGAG CTGGAG(16/14)
BsaAI 422 CACGTG [CT]AC^GT[GA]
BsgI 767 GTGCAG GTGCAG(16/14)
Bsp1286I 460 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 407 TGTACA T^GTACA
BspMI 283 GCAGGT ACCTGC(4/8)
BsrGI 407 TGTACA T^GTACA
BsrI 798 ACTGG ACTGG(1/-1)
BstBI 111 TTCGAA TT^CGAA
CfoI 896 GCGC GCG^C
Cfr13I 792 GGACC G^G[ACGT]CC
DraII 791 GGGACCT [GA]G^G[ACGT]CC[CT]
EarI 700 GAAGAG CTCTTC(1/4)
EcoO109I 791 GGGACCT [GA]G^G[ACGT]CC[CT]
FauI 537 GCGGG CCCGC(4/6)
FbaI 845 TGATCA T^GATCA
HaeII 895 AGCGCC [GA]GCGC^[CT]
HapII 189 CCGG C^CGG
HhaI 896 GCGC GCG^C
HinP1I 896 GCGC G^CGC
HpaII 189 CCGG C^CGG
Ksp632I 700 GAAGAG CTCTTC(1/4)
MslI 72 CACTCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 189 CCGG C^CGG
NlaIII 39 CATG CATG^
NlaIV 791 GGGACC GG[ACGT]^[ACGT]CC
NspV 111 TTCGAA TT^CGAA
PmaCI 422 CACGTG CAC^GTG
PmlI 422 CACGTG CAC^GTG
PpuMI 791 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 10 AACGTT AA^CGTT
PvuII 835 CAGCTG CAG^CTG
Sau96I 792 GGACC G^G[ACGT]CC
SfuI 111 TTCGAA TT^CGAA
SmaI 188 CCCGGG CCC^GGG
SspBI 407 TGTACA T^GTACA
XmaCI 188 CCCGGG C^CCGGG
XmaI 188 CCCGGG C^CCGGG
XmnI 488 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 304 GCGG CCGC(-3/-1)
537 GCGG
AlwI 28 GATCC GGATC(4/5)
541 GGATC
AvaI 188 CCCGGG C^[CT]CG[GA]G
449 CTCGGG
BalI 2 TGGCCA TGG^CCA
228 TGGCCA
BbvCI 781 GCTGAGG CCTCAGC(-5/-2)
868 GCTGAGG
BcnI 188 CCCGG CC^[GC]GG
189 CCGGG
BsaJI 188 CCCGGG C^C[ACGT][ACGT]GG
273 CCGAGG
BsmAI 736 GAGAC GTCTC(1/5)
890 GAGAC
BsoBI 188 CCCGGG C^[CT]CG[GA]G
449 CTCGGG
BstYI 27 AGATCC [GA]^GATC[CT]
541 GGATCT
Cac8I 4 GCCAGC GC[ACGT]^[ACGT]GC
226 GCTGGC
EaeI 2 TGGCCA [CT]^GGCC[GA]
228 TGGCCA
MflI 27 AGATCC [GA]^GATC[CT]
541 GGATCT
MluNI 2 TGGCCA TGG^CCA
228 TGGCCA
MscI 2 TGGCCA TGG^CCA
228 TGGCCA
NciI 188 CCCGG CC^[GC]GG
189 CCGGG
PstI 254 CTGCAG CTGCA^G
299 CTGCAG
TaqI 112 TCGA T^CGA
466 TCGA
TfiI 63 GAATC G^A[AT]TC
622 GATTC
TthHB8I 112 TCGA T^CGA
466 TCGA
XhoII 27 AGATCC [GA]^GATC[CT]
541 GGATCT
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI BbvI BseRI BsmFI
BssKI BstF5I BstNI DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MvaI MwoI
NdeII PleI RsaI Sau3AI ScrFI
SfcI Tru9I TseI Tsp509I