Restriction map for
pFN21AB4535
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbrPI BbsI BbvCI BciVI BclI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI DraI DrdI Eam1105I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MseI
MunI NaeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tsp509I
XbaI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccIII 819 TCCGGA T^CCGGA
AflIII 200 ACGTGT A^C[GA][CT]GT
AlwNI 322 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1077 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 949 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 395 GCAACGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 667 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 819 TCCGGA [AT]^CCGG[AT]
BseAI 819 TCCGGA T^CCGGA
BseRI 490 GAGGAG GAGGAG(10/8)
BsmI 519 GAATGC GAATGC(1/-1)
BspEI 819 TCCGGA T^CCGGA
BstXI 29 CCATGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 323 AGATCC [GA]^GATC[CT]
DraIII 1123 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 254 CGGCCG C^GGCCG
EclXI 254 CGGCCG C^GGCCG
Eco52I 254 CGGCCG C^GGCCG
MflI 323 AGATCC [GA]^GATC[CT]
MroI 819 TCCGGA T^CCGGA
NcoI 1278 CCATGG C^CATGG
NdeI 973 CATATG CA^TATG
PflFI 949 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 321 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 493 GAGTC GAGTC(4/5)
SacI 1201 GAGCTC GAGCT^C
SapI 1203 GCTCTTC GCTCTTC(1/4)
SmaI 121 CCCGGG CCC^GGG
SphI 1171 GCATGC GCATG^C
TfiI 924 GATTC G^A[AT]TC
Tth111I 949 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 321 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 368 CCAACGGTCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 323 AGATCC [GA]^GATC[CT]
XmaCI 121 CCCGGG C^CCGGG
XmaI 121 CCCGGG C^CCGGG
XmnI 1077 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 34 enzymes) #
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AfaI 435 GTAC GT^AC
997 GTAC
AlwI 324 GATCC GGATC(4/5)
810 GATCC
AocI 453 CCTCAGG CC^T[ACGT]AGG
1015 CCTAAGG
Aor51HI 549 AGCGCT AGC^GCT
938 AGCGCT
BalI 26 TGGCCA TGG^CCA
38 TGGCCA
BanII 1183 GAGCCC G[GA]GC[CT]^C
1201 GAGCTC
BfaI 7 CTAG C^TAG
469 CTAG
BsaI 653 GAGACC GGTCTC(1/5)
373 GGTCTC
BsiEI 254 CGGCCG CG[GA][CT]^CG
297 CGACCG
BsmAI 653 GAGAC GTCTC(1/5)
374 GTCTC
BspHI 806 TCATGA T^CATGA
927 TCATGA
BsrFI 71 ACCGGC [GA]^CCGG[CT]
228 GCCGGT
Bsu36I 453 CCTCAGG CC^T[ACGT]AGG
1015 CCTAAGG
Cfr10I 71 ACCGGC [GA]^CCGG[CT]
228 GCCGGT
DsaI 279 CCACGG C^C[GA][CT]GG
1278 CCATGG
EarI 1204 CTCTTC CTCTTC(1/4)
1210 CTCTTC
Eco47III 549 AGCGCT AGC^GCT
938 AGCGCT
Eco81I 453 CCTCAGG CC^T[ACGT]AGG
1015 CCTAAGG
EcoNI 468 CCTAGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
657 CCTGACTGAGG
HgaI 225 GACGC GACGC(5/10)
353 GACGC
HinfI 493 GAGTC G^A[ACGT]TC
924 GATTC
Ksp632I 1204 CTCTTC CTCTTC(1/4)
1210 CTCTTC
MaeI 7 CTAG C^TAG
469 CTAG
MluNI 26 TGGCCA TGG^CCA
38 TGGCCA
MscI 26 TGGCCA TGG^CCA
38 TGGCCA
MspA1I 214 CAGCTG C[AC]G^C[GT]G
595 CAGCTG
PpuMI 563 AGGTCCT [GA]G^G[AT]CC[CT]
632 GGGACCC
PstI 211 CTGCAG CTGCA^G
1228 CTGCAG
PvuII 214 CAGCTG CAG^CTG
595 CAGCTG
RcaI 806 TCATGA T^CATGA
927 TCATGA
RsaI 435 GTAC GT^AC
997 GTAC
SfcI 211 CTGCAG C^T[GA][CT]AG
1228 CTGCAG
SgrAI 70 CACCGGCG C[GA]^CCGG[CT]G
227 CGCCGGTG
StuI 665 AGGCCT AGG^CCT
1139 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI AvaII BanI BbeI BbvI
BcnI BmyI BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI KasI MaeII MaeIII
MboI MboII MnlI MslI MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI Sau3AI
Sau96I ScrFI SfaNI SfoI StyI
TaqI TseI Tsp45I TspRI TthHB8I