(Circular) (Six-base) MAPSORT of: pFN21AB4535.seq Check: 1860 from: 1 to: 6043 pFN21AB4535 6043 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2429 2863 2429 Size: 434 5609 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3853 278 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3386 1937 Size: 1449 4594 Acc65I G'GTAC_C Cuts at: 3364 3364 Size: 6043 AclI AA'CG_TT Cuts at: 1563 4170 4543 1563 Size: 2607 373 3063 Fragments arranged by size: 3063 2607 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2510 3080 3226 4106 5490 5758 1632 Size: 878 570 146 880 1384 268 1917 Fragments arranged by size: 1917 1384 880 878 570 268 146 AfeI AGC'GCT Cuts at: 2556 2945 2556 Size: 389 5654 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5846 AflIII A'CryG_T Cuts at: 1203 2205 4942 5919 1203 Size: 1002 2737 977 1327 Fragments arranged by size: 2737 1327 1002 977 AgeI A'CCGG_T Cuts at: 4874 4874 Size: 6043 AhdI GACnn_n'nnGTC Cuts at: 4772 4772 Size: 6043 AleI CACnn'nnGTG Cuts at: 1166 2924 3285 1166 Size: 1758 361 3924 Fragments arranged by size: 3924 1758 361 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2159 1184 Size: 975 5068 AlwNI CAG_nnn'CTG Cuts at: 2332 3786 5358 2332 Size: 1454 1572 3017 Fragments arranged by size: 3017 1572 1454 ApaLI G'TGCA_C Cuts at: 4100 5256 4100 Size: 1156 4887 ApoI r'AATT_y Cuts at: 1493 3352 3578 3730 5839 1493 Size: 1859 226 152 2109 1697 Fragments arranged by size: 2109 1859 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4597 160 Size: 872 3565 1606 Fragments arranged by size: 3565 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 AvaI C'yCGr_G Cuts at: 1870 1943 2126 2655 3182 3368 1870 Size: 73 183 529 527 186 4545 Fragments arranged by size: 4545 529 527 186 183 73 BamHI G'GATC_C Cuts at: 3373 3740 3373 Size: 367 5676 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2023 2079 2190 2427 3253 Size: 325 217 627 236 56 111 237 826 Cuts at: 3253 3364 4819 618 Size: 111 1455 1842 Fragments arranged by size: 1842 1455 826 627 325 237 236 217 111 111 56 BanII G_rGCy'C Cuts at: 1391 3192 3210 3362 1391 Size: 1801 18 152 4072 Fragments arranged by size: 4072 1801 152 18 BbeI G_GCGC'C Cuts at: 2083 2194 2431 2083 Size: 111 237 5695 Fragments arranged by size: 5695 237 111 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5072 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2389 2423 4234 4268 1903 Size: 34 452 34 1811 34 3678 Fragments arranged by size: 3678 1811 452 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3935 5145 3935 Size: 1210 4833 BclI T'GATC_A Cuts at: 1548 1548 Size: 6043 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2678 4654 136 Size: 107 122 71 631 605 1006 1976 1525 Fragments arranged by size: 1976 1525 1006 631 605 122 107 71 BglII A'GATC_T Cuts at: 6038 6038 Size: 6043 BlpI GC'TnA_GC Cuts at: 3460 3460 Size: 6043 Bme1580I G_kGCm'C Cuts at: 1790 2028 2319 3258 3339 4104 5260 1790 Size: 238 291 939 81 765 1156 2573 Fragments arranged by size: 2573 1156 939 765 291 238 81 BmgBI CAC'GTC Cuts at: 1318 2206 2863 1318 Size: 888 657 4498 Fragments arranged by size: 4498 888 657 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3774 4732 442 Size: 416 53 83 399 24 2357 958 1753 Fragments arranged by size: 2357 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4703 1239 Size: 3464 2579 Bpu10I CC'TnA_GC Cuts at: 2478 2478 Size: 6043 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4168 5033 5331 4168 Size: 865 298 4880 Fragments arranged by size: 4880 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2384 2652 4706 882 Size: 617 885 268 2054 2219 Fragments arranged by size: 2219 2054 885 617 268 BsaAI yAC'GTr Cuts at: 493 1204 3834 493 Size: 711 2630 2702 Fragments arranged by size: 2702 2630 711 BsaBI GATnn'nnATC Cuts at: 6037 6037 Size: 6043 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2080 2191 2231 2359 Size: 53 83 186 936 547 111 40 128 Cuts at: 2359 2428 3850 4232 4867 275 Size: 69 1422 382 635 1451 Fragments arranged by size: 1451 1422 936 635 547 382 186 128 111 83 69 53 40 BsaWI w'CCGG_w Cuts at: 2824 4476 4874 5148 5295 5706 2824 Size: 1652 398 274 147 411 3161 Fragments arranged by size: 3161 1652 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5818 Fragments arranged by size: 5818 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2510 1186 Size: 1324 4719 BseYI C'CCAG_C Cuts at: 2088 2131 2551 2598 2641 2646 3275 5246 5742 Size: 43 420 47 43 5 629 1971 496 Cuts at: 5742 2088 Size: 2389 Fragments arranged by size: 2389 1971 629 496 420 47 43 43 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2079 2852 2960 1163 Size: 573 343 773 108 4246 Fragments arranged by size: 4246 773 573 343 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2262 2305 3493 4254 5282 1540 Size: 275 186 261 43 1188 761 1028 2301 Fragments arranged by size: 2301 1188 1028 761 275 261 186 43 BsiHKAI G_wGCw'C Cuts at: 2247 2625 3210 4104 4189 5260 2247 Size: 378 585 894 85 1071 3030 Fragments arranged by size: 3030 1071 894 585 378 85 BsmI GAATG_Cn' Cuts at: 2530 3554 3647 2530 Size: 1024 93 4926 Fragments arranged by size: 4926 1024 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2247 2319 2625 3192 3210 3258 Size: 399 238 219 72 306 567 18 48 Cuts at: 3258 3339 3362 4104 4189 5260 1391 Size: 81 23 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 567 399 306 238 219 85 81 72 48 23 18 BspEI T'CCGG_A Cuts at: 2824 5706 2824 Size: 2882 3161 BspHI T'CATG_A Cuts at: 2811 2932 3932 5662 2811 Size: 121 1000 1730 3192 Fragments arranged by size: 3192 1730 1000 121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2148 2429 2863 3398 844 Size: 1304 281 434 535 3489 Fragments arranged by size: 3489 1304 535 434 281 BsrBI CCG'CTC Cuts at: 1394 1823 3930 1394 Size: 429 2107 3507 Fragments arranged by size: 3507 2107 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4538 4712 65 Size: 1183 3290 174 1396 Fragments arranged by size: 3290 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2076 2233 4687 4874 1700 Size: 110 266 157 2454 187 2869 Fragments arranged by size: 2869 2454 266 187 157 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6043 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6043 BssSI C'ACGA_G Cuts at: 4103 5115 4103 Size: 1012 5031 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6043 BstBI TT'CG_AA Cuts at: 4847 4847 Size: 6043 BstEII G'GTnAC_C Cuts at: 1184 2278 2773 2894 3304 1184 Size: 1094 495 121 410 3923 Fragments arranged by size: 3923 1094 495 410 121 BstXI CCAn_nnnn'nTGG Cuts at: 2041 2041 Size: 6043 BstYI r'GATC_y Cuts at: 1972 2328 3373 3740 4125 4142 5594 5680 5692 Size: 356 1045 367 385 17 1452 86 12 Cuts at: 5692 6038 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1045 385 367 356 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2459 3021 2459 Size: 562 5481 BtgI C'CryG_G Cuts at: 513 1065 2004 2284 3283 513 Size: 552 939 280 999 3273 Fragments arranged by size: 3273 999 939 552 280 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5304 Fragments arranged by size: 5304 683 56 BtsI GCAGTG_nn' Cuts at: 2120 2919 3559 4353 4373 2120 Size: 799 640 794 20 3790 Fragments arranged by size: 3790 799 794 640 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6043 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6008 DraI TTT'AAA Cuts at: 3347 3347 Size: 6043 DraIII CAC_nnn'GTG Cuts at: 3133 3133 Size: 6043 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5050 809 Size: 507 3734 1802 Fragments arranged by size: 3734 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2043 2259 Size: 54 1425 50 275 18 201 12 216 Cuts at: 2259 3490 4379 6008 8 Size: 1231 889 1629 43 Fragments arranged by size: 1629 1425 1231 889 275 216 201 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2259 3490 1537 Size: 275 447 1231 4090 Fragments arranged by size: 4090 1231 447 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3215 3221 3973 1353 Size: 1862 6 752 3423 Fragments arranged by size: 3423 1862 752 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4630 5004 5150 387 Size: 1064 138 3041 374 146 1280 Fragments arranged by size: 3041 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3208 3208 Size: 6043 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2510 3080 3226 4106 4703 5490 5758 Size: 393 878 570 146 880 597 787 268 Cuts at: 5758 1239 Size: 1524 Fragments arranged by size: 1524 880 878 787 597 570 393 268 146 EcoNI CCTnn'n_nnAGG Cuts at: 2477 2666 2477 Size: 189 5854 EcoO109I rG'GnC_Cy Cuts at: 2111 2569 2638 2800 2111 Size: 458 69 162 5354 Fragments arranged by size: 5354 458 162 69 EcoRI G'AATT_C Cuts at: 3352 3730 3352 Size: 378 5665 FspI TGC'GCA Cuts at: 4549 4549 Size: 6043 HaeII r_GCGC'y Cuts at: 1678 2083 2194 2276 2431 2558 2839 2947 5190 Size: 405 111 82 155 127 281 108 2243 Cuts at: 5190 1678 Size: 2531 Fragments arranged by size: 2531 2243 405 281 155 127 111 108 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2744 2776 2806 2838 4684 Size: 32 135 32 1172 32 30 32 1846 Cuts at: 4684 4716 4758 4790 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1846 1172 135 42 32 32 32 32 32 32 30 HincII GTy'rAC Cuts at: 669 1657 1938 3387 3633 669 Size: 988 281 1449 246 3079 Fragments arranged by size: 3079 1449 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6043 HpaI GTT'AAC Cuts at: 3633 3633 Size: 6043 KasI G'GCGC_C Cuts at: 2079 2190 2427 2079 Size: 111 237 5695 Fragments arranged by size: 5695 237 111 KpnI G_GTAC'C Cuts at: 3368 3368 Size: 6043 MfeI C'AATT_G Cuts at: 3642 3642 Size: 6043 MluI A'CGCG_T Cuts at: 5919 5919 Size: 6043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2869 5157 5341 1305 Size: 1564 2288 184 2007 Fragments arranged by size: 2288 2007 1564 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2033 2045 10 Size: 54 1425 343 201 12 4008 Fragments arranged by size: 4008 1425 343 201 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2744 2826 2924 3285 4001 4360 Size: 648 873 705 82 98 361 716 359 Cuts at: 4360 4519 518 Size: 159 2042 Fragments arranged by size: 2042 873 716 705 648 361 359 159 98 82 MspA1I CmG'CkG Cuts at: 1606 2221 2602 3459 4136 5284 5529 1606 Size: 615 381 857 677 1148 245 2120 Fragments arranged by size: 2120 1148 857 677 615 381 245 NarI GG'CG_CC Cuts at: 2080 2191 2428 2080 Size: 111 237 5695 Fragments arranged by size: 5695 237 111 NcoI C'CATG_G Cuts at: 513 1065 2004 3283 513 Size: 552 939 1279 3273 Fragments arranged by size: 3273 1279 939 552 NdeI CA'TA_TG Cuts at: 387 2979 387 Size: 2592 3451 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6043 NotI GC'GGCC_GC Cuts at: 3490 3490 Size: 6043 NspI r_CATG'y Cuts at: 1132 1229 2150 2911 3180 3401 4946 1132 Size: 97 921 761 269 221 1545 2229 Fragments arranged by size: 2229 1545 921 761 269 221 97 PasI CC'CwG_GG Cuts at: 1348 2965 3121 1348 Size: 1617 156 4270 Fragments arranged by size: 4270 1617 156 PciI A'CATG_T Cuts at: 4942 4942 Size: 6043 PflMI CCAn_nnn'nTGG Cuts at: 1108 2332 1108 Size: 1224 4819 PfoI T'CCnGG_A Cuts at: 3111 3111 Size: 6043 PmeI GTTT'AAAC Cuts at: 3347 3347 Size: 6043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4112 4144 5625 5657 4112 Size: 32 1481 32 4498 Fragments arranged by size: 4498 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2569 2638 2569 Size: 69 5974 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6043 PsiI TTA'TAA Cuts at: 3613 3613 Size: 6043 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2153 2185 2153 Size: 32 6011 PstI C_TGCA'G Cuts at: 830 2220 3237 3395 830 Size: 1390 1017 158 3478 Fragments arranged by size: 3478 1390 1017 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6043 PvuII CAG'CTG Cuts at: 2221 2602 2221 Size: 381 5662 SacI G_AGCT'C Cuts at: 3210 3210 Size: 6043 SalI G'TCGA_C Cuts at: 1936 3385 1936 Size: 1449 4594 SanDI GG'GwC_CC Cuts at: 2638 2638 Size: 6043 SapI GCTCTTCn'nnn_ Cuts at: 3215 3215 Size: 6043 SbfI CC_TGCA'GG Cuts at: 3395 3395 Size: 6043 ScaI AGT'ACT Cuts at: 4291 6003 4291 Size: 1712 4331 SfcI C'TryA_G Cuts at: 826 1045 2216 3233 3391 4526 5207 5398 826 Size: 219 1171 1017 158 1135 681 191 1471 Fragments arranged by size: 1471 1171 1135 1017 681 219 191 158 SfoI GGC'GCC Cuts at: 2081 2192 2429 2081 Size: 111 237 5695 Fragments arranged by size: 5695 237 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 SgrAI Cr'CCGG_yG Cuts at: 1700 2076 2233 1700 Size: 376 157 5510 Fragments arranged by size: 5510 376 157 SmaI CCC'GGG Cuts at: 1872 2128 3370 1872 Size: 256 1242 4545 Fragments arranged by size: 4545 1242 256 SmlI C'TyrA_G Cuts at: 820 1017 1943 4147 5048 5310 820 Size: 197 926 2204 901 262 1553 Fragments arranged by size: 2204 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3834 493 Size: 3341 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6043 SphI G_CATG'C Cuts at: 1132 3180 3401 1132 Size: 2048 221 3774 Fragments arranged by size: 3774 2048 221 SrfI GCCC'GGGC Cuts at: 2128 2128 Size: 6043 SspI AAT'ATT Cuts at: 5 52 3967 5 Size: 47 3915 2081 Fragments arranged by size: 3915 2081 47 StuI AGG'CCT Cuts at: 1517 2672 3146 1517 Size: 1155 474 4414 Fragments arranged by size: 4414 1155 474 StyI C'CwwG_G Cuts at: 513 1065 2004 2353 2697 3283 3482 513 Size: 552 939 349 344 586 199 3074 Fragments arranged by size: 3074 939 586 552 349 344 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2339 2882 2957 4070 4087 4240 4425 Size: 1255 759 543 75 1113 17 153 185 Cuts at: 4425 4870 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1113 759 543 445 185 153 75 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3001 4289 6001 Size: 274 80 33 51 1443 1024 1288 1712 Cuts at: 6001 96 Size: 138 Fragments arranged by size: 1712 1443 1288 1024 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6003 6035 6003 Size: 32 6011 Tth111I GACn'n_nGTC Cuts at: 1316 2957 1316 Size: 1641 4402 XbaI T'CTAG_A Cuts at: 3379 3379 Size: 6043 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2380 2380 Size: 6043 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6043 XmaI C'CCGG_G Cuts at: 1870 2126 3368 1870 Size: 256 1242 4545 Fragments arranged by size: 4545 1242 256 XmnI GAAnn'nnTTC Cuts at: 3086 4172 3086 Size: 1086 4957 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3851 276 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SexAI SfiI SwaI