(Circular) (Six-base) MAPSORT of: pFN21AB4375.seq Check: 8402 from: 1 to: 5872 pFN21AB4375 5872 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2175 2175 Size: 5872 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3682 278 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2925 3215 1937 Size: 988 290 4594 Fragments arranged by size: 4594 988 290 Acc65I G'GTAC_C Cuts at: 3193 3193 Size: 5872 AclI AA'CG_TT Cuts at: 1563 3999 4372 1563 Size: 2436 373 3063 Fragments arranged by size: 3063 2436 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2466 3935 5319 5587 1632 Size: 834 1469 1384 268 1917 Fragments arranged by size: 1917 1469 1384 834 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5675 AflIII A'CryG_T Cuts at: 1203 4771 5748 1203 Size: 3568 977 1327 Fragments arranged by size: 3568 1327 977 AgeI A'CCGG_T Cuts at: 4703 4703 Size: 5872 AhdI GACnn_n'nnGTC Cuts at: 4601 4601 Size: 5872 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5872 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3067 1184 Size: 1883 3989 AlwNI CAG_nnn'CTG Cuts at: 3615 5187 3615 Size: 1572 4300 ApaI G_GGCC'C Cuts at: 2095 2095 Size: 5872 ApaLI G'TGCA_C Cuts at: 2992 3092 3929 5085 2992 Size: 100 837 1156 3779 Fragments arranged by size: 3779 1156 837 100 ApoI r'AATT_y Cuts at: 1493 2413 2607 2625 3181 3407 3559 5668 1493 Size: 920 194 18 556 226 152 2109 1697 Fragments arranged by size: 2109 1697 920 556 226 194 152 18 AseI AT'TA_AT Cuts at: 160 1032 4426 160 Size: 872 3394 1606 Fragments arranged by size: 3394 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 AvaI C'yCGr_G Cuts at: 1870 1943 2100 2245 2733 3197 1870 Size: 73 157 145 488 464 4545 Fragments arranged by size: 4545 488 464 157 145 73 BamHI G'GATC_C Cuts at: 3202 3569 3202 Size: 367 5505 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 2258 2267 2394 2900 Size: 325 217 627 462 9 9 127 506 Cuts at: 2900 3193 4648 618 Size: 293 1455 1842 Fragments arranged by size: 1842 1455 627 506 462 325 293 217 127 9 9 BanII G_rGCy'C Cuts at: 1391 2095 3191 1391 Size: 704 1096 4072 Fragments arranged by size: 4072 1096 704 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4901 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4063 4097 1903 Size: 34 2126 34 3678 Fragments arranged by size: 3678 2126 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3764 4974 3764 Size: 1210 4662 BclI T'GATC_A Cuts at: 1548 1548 Size: 5872 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2241 2286 4483 Size: 107 122 71 631 605 569 45 2197 Cuts at: 4483 136 Size: 1525 Fragments arranged by size: 2197 1525 631 605 569 122 107 71 45 BglII A'GATC_T Cuts at: 5867 5867 Size: 5872 BlpI GC'TnA_GC Cuts at: 3289 3289 Size: 5872 Bme1580I G_kGCm'C Cuts at: 1790 2095 2252 2397 2996 3096 3933 5089 1790 Size: 305 157 145 599 100 837 1156 2573 Fragments arranged by size: 2573 1156 837 599 305 157 145 100 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5872 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2504 3603 4561 Size: 416 53 83 399 24 1087 1099 958 Cuts at: 4561 442 Size: 1753 Fragments arranged by size: 1753 1099 1087 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5872 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2215 2215 Size: 5872 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2550 2568 2646 2681 2903 4532 1239 Size: 1311 18 78 35 222 1629 2579 Fragments arranged by size: 2579 1629 1311 222 78 35 18 Bpu10I CC'TnA_GC Cuts at: 2273 2337 2273 Size: 64 5808 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2664 3044 3997 4862 5160 2664 Size: 380 953 865 298 3376 Fragments arranged by size: 3376 953 865 380 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2832 4535 882 Size: 617 1333 1703 2219 Fragments arranged by size: 2219 1703 1333 617 BsaAI yAC'GTr Cuts at: 493 1204 3663 493 Size: 711 2459 2702 Fragments arranged by size: 2702 2459 711 BsaBI GATnn'nnATC Cuts at: 5866 5866 Size: 5872 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3048 3679 4061 4696 Size: 53 83 186 936 1515 631 382 635 Cuts at: 4696 275 Size: 1451 Fragments arranged by size: 1515 1451 936 635 631 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4305 4703 4977 5124 5535 4305 Size: 398 274 147 411 4642 Fragments arranged by size: 4642 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2366 2396 1178 Size: 30 165 30 963 30 4654 Fragments arranged by size: 4654 963 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3047 1186 Size: 1861 4011 BseYI C'CCAG_C Cuts at: 3151 5075 5571 3151 Size: 1924 496 3452 Fragments arranged by size: 3452 1924 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5299 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3322 4083 5111 1540 Size: 275 186 1321 761 1028 2301 Fragments arranged by size: 2301 1321 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2817 2996 3096 3933 4018 5089 2817 Size: 179 100 837 85 1071 3600 Fragments arranged by size: 3600 1071 837 179 100 85 BsmI GAATG_Cn' Cuts at: 3383 3476 3383 Size: 93 5779 BsmBI CGTCTCn'nnnn_ Cuts at: 3002 3002 Size: 5872 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2095 2252 2397 2817 2996 3096 3191 Size: 399 305 157 145 420 179 100 95 Cuts at: 3191 3933 4018 5089 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 420 399 305 179 157 145 100 95 85 BspEI T'CCGG_A Cuts at: 5535 5535 Size: 5872 BspHI T'CATG_A Cuts at: 3761 5491 3761 Size: 1730 4142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 3227 844 Size: 1331 1052 3489 Fragments arranged by size: 3489 1331 1052 BsrBI CCG'CTC Cuts at: 1394 1823 3759 1394 Size: 429 1936 3507 Fragments arranged by size: 3507 1936 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2497 4367 4541 65 Size: 1183 1249 1870 174 1396 Fragments arranged by size: 1870 1396 1249 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4516 4703 1700 Size: 110 2706 187 2869 Fragments arranged by size: 2869 2706 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5872 BssSI C'ACGA_G Cuts at: 3932 4944 3932 Size: 1012 4860 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5872 BstBI TT'CG_AA Cuts at: 4676 4676 Size: 5872 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5872 BstXI CCAn_nnnn'nTGG Cuts at: 3151 3151 Size: 5872 BstYI r'GATC_y Cuts at: 1972 3202 3569 3954 3971 5423 5509 5521 5867 Size: 1230 367 385 17 1452 86 12 346 Cuts at: 5867 1972 Size: 1977 Fragments arranged by size: 1977 1452 1230 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2926 2926 Size: 5872 BtgI C'CryG_G Cuts at: 513 1065 2004 2054 513 Size: 552 939 50 4331 Fragments arranged by size: 4331 939 552 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5133 Fragments arranged by size: 5133 683 56 BtsI GCAGTG_nn' Cuts at: 2996 3388 4182 4202 2996 Size: 392 794 20 4666 Fragments arranged by size: 4666 794 392 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5872 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3118 3153 529 Size: 35 2554 35 3248 Fragments arranged by size: 3248 2554 35 35 DraI TTT'AAA Cuts at: 3176 3176 Size: 5872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2655 4879 809 Size: 507 1339 2224 1802 Fragments arranged by size: 2224 1802 1339 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3319 4208 5837 Size: 54 1425 50 275 18 1489 889 1629 Cuts at: 5837 8 Size: 43 Fragments arranged by size: 1629 1489 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3319 1537 Size: 275 1507 4090 Fragments arranged by size: 4090 1507 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2135 3802 1353 Size: 782 1667 3423 Fragments arranged by size: 3423 1667 782 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4459 4833 4979 387 Size: 1064 138 2870 374 146 1280 Fragments arranged by size: 2870 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2466 2550 2568 2646 2681 2903 3935 Size: 393 834 84 18 78 35 222 1032 Cuts at: 3935 4532 5319 5587 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1032 834 787 597 393 268 222 84 78 35 18 EcoNI CCTnn'n_nnAGG Cuts at: 3059 3059 Size: 5872 EcoO109I rG'GnC_Cy Cuts at: 2091 2092 2543 2570 2579 2588 2597 2666 2684 Size: 1 451 27 9 9 9 69 18 Cuts at: 2684 2702 2091 Size: 18 5261 Fragments arranged by size: 5261 451 69 27 18 18 9 9 9 1 EcoRI G'AATT_C Cuts at: 2607 2625 3181 3559 2607 Size: 18 556 378 4920 Fragments arranged by size: 4920 556 378 18 FspI TGC'GCA Cuts at: 4378 4378 Size: 5872 HaeII r_GCGC'y Cuts at: 1678 2054 5019 1678 Size: 376 2965 2531 Fragments arranged by size: 2965 2531 376 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4513 4545 4587 4619 1373 Size: 32 135 32 2941 32 42 32 2626 Fragments arranged by size: 2941 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3216 3462 669 Size: 988 281 1278 246 3079 Fragments arranged by size: 3079 1278 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5872 HpaI GTT'AAC Cuts at: 3462 3462 Size: 5872 KpnI G_GTAC'C Cuts at: 3197 3197 Size: 5872 MfeI C'AATT_G Cuts at: 3471 3471 Size: 5872 MluI A'CGCG_T Cuts at: 5748 5748 Size: 5872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4986 5170 1305 Size: 3681 184 2007 Fragments arranged by size: 3681 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4050 Fragments arranged by size: 4050 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2837 3113 3830 4189 4348 518 Size: 648 1671 276 717 359 159 2042 Fragments arranged by size: 2042 1671 717 648 359 276 159 MspA1I CmG'CkG Cuts at: 1606 2056 2121 2750 2874 3288 3965 5113 5358 Size: 450 65 629 124 414 677 1148 245 Cuts at: 5358 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 629 450 414 245 124 65 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4381 Fragments arranged by size: 4381 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5872 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5872 NotI GC'GGCC_GC Cuts at: 3319 3319 Size: 5872 NspI r_CATG'y Cuts at: 1132 1229 3230 4775 1132 Size: 97 2001 1545 2229 Fragments arranged by size: 2229 2001 1545 97 PasI CC'CwG_GG Cuts at: 1348 2593 1348 Size: 1245 4627 PciI A'CATG_T Cuts at: 4771 4771 Size: 5872 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5872 PfoI T'CCnGG_A Cuts at: 2548 2566 2584 2620 2629 2548 Size: 18 18 36 9 5791 Fragments arranged by size: 5791 36 18 18 9 PmeI GTTT'AAAC Cuts at: 3176 3176 Size: 5872 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3941 3973 5454 5486 3941 Size: 32 1481 32 4327 Fragments arranged by size: 4327 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2543 2570 2579 2588 2597 2666 2684 2702 2543 Size: 27 9 9 9 69 18 18 5713 Fragments arranged by size: 5713 69 27 18 18 9 9 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5872 PsiI TTA'TAA Cuts at: 3442 3442 Size: 5872 PspOMI G'GGCC_C Cuts at: 2091 2091 Size: 5872 PstI C_TGCA'G Cuts at: 830 2047 2991 3224 830 Size: 1217 944 233 3478 Fragments arranged by size: 3478 1217 944 233 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5872 PvuII CAG'CTG Cuts at: 2121 2750 2121 Size: 629 5243 SacII CC_GC'GG Cuts at: 2057 2057 Size: 5872 SalI G'TCGA_C Cuts at: 1936 3214 1936 Size: 1278 4594 SanDI GG'GwC_CC Cuts at: 2597 2597 Size: 5872 SapI GCTCTTCn'nnn_ Cuts at: 2135 2135 Size: 5872 SbfI CC_TGCA'GG Cuts at: 3224 3224 Size: 5872 ScaI AGT'ACT Cuts at: 4120 5832 4120 Size: 1712 4160 SexAI A'CCwGG_T Cuts at: 2653 2653 Size: 5872 SfcI C'TryA_G Cuts at: 826 1045 2043 2762 2914 2987 3220 4355 5036 Size: 219 998 719 152 73 233 1135 681 Cuts at: 5036 5227 826 Size: 191 1471 Fragments arranged by size: 1471 1135 998 719 681 233 219 191 152 73 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5872 SmaI CCC'GGG Cuts at: 1872 2102 3199 1872 Size: 230 1097 4545 Fragments arranged by size: 4545 1097 230 SmlI C'TyrA_G Cuts at: 820 1017 1943 2679 2733 3059 3976 4877 5139 Size: 197 926 736 54 326 917 901 262 Cuts at: 5139 820 Size: 1553 Fragments arranged by size: 1553 926 917 901 736 326 262 197 54 SnaBI TAC'GTA Cuts at: 493 3663 493 Size: 3170 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5872 SphI G_CATG'C Cuts at: 1132 3230 1132 Size: 2098 3774 SrfI GCCC'GGGC Cuts at: 2102 2102 Size: 5872 SspI AAT'ATT Cuts at: 5 52 3796 5 Size: 47 3744 2081 Fragments arranged by size: 3744 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5872 StyI C'CwwG_G Cuts at: 513 1065 2004 2236 2320 2770 2847 3311 513 Size: 552 939 232 84 450 77 464 3074 Fragments arranged by size: 3074 939 552 464 450 232 84 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3143 3899 3916 4069 4254 4699 325 Size: 1255 1563 756 17 153 185 445 1498 Fragments arranged by size: 1563 1498 1255 756 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4118 5830 96 Size: 274 80 33 51 1443 2141 1712 138 Fragments arranged by size: 2141 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2366 2398 5832 5864 2366 Size: 32 3434 32 2374 Fragments arranged by size: 3434 2374 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5872 XbaI T'CTAG_A Cuts at: 3208 3208 Size: 5872 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2705 2959 2705 Size: 254 5618 XhoI C'TCGA_G Cuts at: 1943 2733 1943 Size: 790 5082 XmaI C'CCGG_G Cuts at: 1870 2100 3197 1870 Size: 230 1097 4545 Fragments arranged by size: 4545 1097 230 XmnI GAAnn'nnTTC Cuts at: 2417 2981 4001 2417 Size: 564 1020 4288 Fragments arranged by size: 4288 1020 564 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3680 276 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SfiI SfoI SwaI