Restriction map for
pFN21AB4163
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BbvCI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsiWI BsmI
Bsp1407I BspLU11I BsrDI BsrGI BssHII
BssSI BstBI CelII CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI KspI
MfeI MluI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 377 TCCGGA T^CCGGA
AflIII 2783 ACGTGT A^C[GA][CT]GT
AocI 1324 CCTGAGG CC^T[ACGT]AGG
Asp700I 963 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 1580 GGCGCC GGCGC^C
BbrPI 2782 CACGTG CAC^GTG
BbsI 2443 GTCTTC GAAGAC(2/6)
BfaI 202 CTAG C^TAG
BpuAI 2443 GTCTTC GAAGAC(2/6)
BsaBI 657 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 377 TCCGGA [AT]^CCGG[AT]
BseAI 377 TCCGGA T^CCGGA
BsiEI 699 CGACCG CG[GA][CT]^CG
BsmBI 1651 GAGACG CGTCTC(1/5)
BspEI 377 TCCGGA T^CCGGA
BsrFI 2504 ACCGGC [GA]^CCGG[CT]
Bst1107I 205 GTATAC GTA^TAC
BstAPI 2587 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 205 GTATAC GTA^TAC
Bsu36I 1324 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2504 ACCGGC [GA]^CCGG[CT]
Eco81I 1324 CCTGAGG CC^T[ACGT]AGG
HgaI 1760 GCGTC GACGC(5/10)
KasI 1580 GGCGCC G^GCGCC
MaeI 202 CTAG C^TAG
MamI 657 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 377 TCCGGA T^CCGGA
MseI 494 TTAA T^TAA
NarI 1580 GGCGCC GG^CGCC
PmaCI 2782 CACGTG CAC^GTG
PmlI 2782 CACGTG CAC^GTG
PshAI 1200 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 61 GAGCTC GAGCT^C
SfoI 1580 GGCGCC GGC^GCC
SmaI 1331 CCCGGG CCC^GGG
SphI 170 GCATGC GCATG^C
SspI 14 AATATT AAT^ATT
TfiI 1302 GAATC G^A[AT]TC
Tru9I 494 TTAA T^TAA
XmaCI 1331 CCCGGG C^CCGGG
XmaI 1331 CCCGGG C^CCGGG
XmnI 963 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 205 GTATAC GT^[AC][GT]AC
1717 GTCTAC
AcyI 1580 GGCGCC G[GA]^CG[CT]C
1759 GGCGTC
Alw44I 639 GTGCAC G^TGCAC
1765 GTGCAC
Aor51HI 546 AGCGCT AGC^GCT
1166 AGCGCT
ApaLI 639 GTGCAC G^TGCAC
1765 GTGCAC
AviII 410 TGCGCA TGC^GCA
908 TGCGCA
BamHI 399 GGATCC G^GATCC
2717 GGATCC
BsaAI 997 TACGTG [CT]AC^GT[GA]
2782 CACGTG
BsaHI 1580 GGCGCC G[GA]^CG[CT]C
1759 GGCGTC
BsaI 1492 GAGACC GGTCTC(1/5)
2800 GAGACC
BseRI 1894 CTCCTC GAGGAG(10/8)
1944 CTCCTC
BspDI 117 ATCGAT AT^CGAT
2437 ATCGAT
BsrBI 724 GAGCGG CCGCTC(-3/-3)
1923 GAGCGG
BstEII 729 GGTCACC G^GT[ACGT]ACC
1888 GGTGACC
BstPI 729 GGTCACC G^GT[ACGT]ACC
1888 GGTGACC
ClaI 117 ATCGAT AT^CGAT
2437 ATCGAT
DraII 287 AGGACCC [GA]G^G[ACGT]CC[CT]
1514 AGGTCCT
EarI 148 CTCTTC CTCTTC(1/4)
1648 GAAGAG
Eco47III 546 AGCGCT AGC^GCT
1166 AGCGCT
EcoO109I 287 AGGACCC [GA]G^G[ACGT]CC[CT]
1514 AGGTCCT
EcoO65I 729 GGTCACC G^GT[ACGT]ACC
1888 GGTGACC
FspI 410 TGCGCA TGC^GCA
908 TGCGCA
Hin1I 1580 GGCGCC G[GA]^CG[CT]C
1759 GGCGTC
Ksp632I 148 CTCTTC CTCTTC(1/4)
1648 GAAGAG
NdeI 404 CATATG CA^TATG
2123 CATATG
NspI 170 GCATGC [GA]CATG^[CT]
2660 ACATGC
PaeR7I 1408 CTCGAG C^TCGAG
1794 CTCGAG
PpuMI 287 AGGACCC [GA]G^G[AT]CC[CT]
1514 AGGTCCT
SfiI 249 GGCCATTATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1035 GGCCTCTCAGGCC
Tsp509I 223 AATT ^AATT
558 AATT
XhoI 1408 CTCGAG C^TCGAG
1794 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbvI BcgI BcnI
BglI BglII BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraIII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII