Restriction map for
pFN21AB4163
(Linear) (Six-base) MAPSORT of: KIBB4163.seq Check: 8880 from: 1 to: 2817
KIBB4163.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AccI GT'mk_AC
Cuts at: 0 206 1718 2817
Size: 206 1512 1099
Fragments arranged by size:
1512 1099 206
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 214 409 970 1667 2436 2698 2773
Size: 73 141 195 561 697 769 262 75
Cuts at: 2773 2817
Size: 44
Fragments arranged by size:
769 697 561 262 195 141 75 73
44
AfeI AGC'GCT
Cuts at: 0 548 1168 2817
Size: 548 620 1649
Fragments arranged by size:
1649 620 548
AflIII A'CryG_T
Cuts at: 0 2783 2817
Size: 2783 34
AleI CACnn'nnGTG
Cuts at: 0 271 2817
Size: 271 2546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 444 601 2623 2716 2817
Size: 444 157 2022 93 101
Fragments arranged by size:
2022 444 157 101 93
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 589 621 2817
Size: 589 32 2196
Fragments arranged by size:
2196 589 32
AlwNI CAG_nnn'CTG
Cuts at: 0 424 1452 1503 2573 2593 2676 2817
Size: 424 1028 51 1070 20 83 141
Fragments arranged by size:
1070 1028 424 141 83 51 20
ApaLI G'TGCA_C
Cuts at: 0 639 1765 2817
Size: 639 1126 1052
Fragments arranged by size:
1126 1052 639
AvaI C'yCGr_G
Cuts at: 0 1331 1408 1794 2817
Size: 1331 77 386 1023
Fragments arranged by size:
1331 1023 386 77
BamHI G'GATC_C
Cuts at: 0 399 2717 2817
Size: 399 2318 100
Fragments arranged by size:
2318 399 100
BanI G'GyrC_C
Cuts at: 0 1580 2005 2019 2107 2185 2817
Size: 1580 425 14 88 78 632
Fragments arranged by size:
1580 632 425 88 78 14
BanII G_rGCy'C
Cuts at: 0 65 312 1670 1816 2209 2383 2817
Size: 65 247 1358 146 393 174 434
Fragments arranged by size:
1358 434 393 247 174 146 65
BbeI G_GCGC'C
Cuts at: 0 1584 2817
Size: 1584 1233
BbsI GAAGACnn'nnnn_
Cuts at: 0 2436 2817
Size: 2436 381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 570 604 1167 1201 1995 2029 2817
Size: 570 34 563 34 794 34 788
Fragments arranged by size:
794 788 570 563 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 256 1042 2161 2204 2817
Size: 256 786 1119 43 613
Fragments arranged by size:
1119 786 613 256 43
BglII A'GATC_T
Cuts at: 0 41 656 2177 2354 2817
Size: 41 615 1521 177 463
Fragments arranged by size:
1521 615 463 177 41
Bme1580I G_kGCm'C
Cuts at: 0 147 643 1769 2008 2110 2817
Size: 147 496 1126 239 102 707
Fragments arranged by size:
1126 707 496 239 147 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 1727 1912 2550 2817
Size: 1727 185 638 267
Fragments arranged by size:
1727 638 267 185
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1027 1417 1853 2194 2299 2398 2817
Size: 208 819 390 436 341 105 99 419
Fragments arranged by size:
819 436 419 390 341 208 105 99
Bpu10I CC'TnA_GC
Cuts at: 0 2165 2817
Size: 2165 652
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 2794 2817
Size: 826 1968 23
Fragments arranged by size:
1968 826 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 1486 2794 2817
Size: 1486 1308 23
Fragments arranged by size:
1486 1308 23
BsaAI yAC'GTr
Cuts at: 0 999 2784 2817
Size: 999 1785 33
Fragments arranged by size:
1785 999 33
BsaBI GATnn'nnATC
Cuts at: 0 661 2817
Size: 661 2156
BsaHI Gr'CG_yC
Cuts at: 0 1581 1760 2817
Size: 1581 179 1057
Fragments arranged by size:
1581 1057 179
BsaWI w'CCGG_w
Cuts at: 0 377 2817
Size: 377 2440
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 490 520 793 823 1606 1636 2817
Size: 490 30 273 30 783 30 1181
Fragments arranged by size:
1181 783 490 273 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1885 1935 2817
Size: 1885 50 882
Fragments arranged by size:
1885 882 50
BseYI C'CCAG_C
Cuts at: 0 458 574 1884 2151 2734 2777 2817
Size: 458 116 1310 267 583 43 40
Fragments arranged by size:
1310 583 458 267 116 43 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1528 1972 2455 2724 2817
Size: 949 579 444 483 269 93
Fragments arranged by size:
949 579 483 444 269 93
BsiEI CG_ry'CG
Cuts at: 0 702 2817
Size: 702 2115
BsiHKAI G_wGCw'C
Cuts at: 0 65 643 770 1180 1432 1769 1839 2058
Size: 65 578 127 410 252 337 70 219
Cuts at: 2058 2766 2817
Size: 708 51
Fragments arranged by size:
708 578 410 337 252 219 127 70
65 51
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1645 2817
Size: 1645 1172
Bsp1286I G_dGCh'C
Cuts at: 0 65 147 312 643 770 1180 1432 1670
Size: 65 82 165 331 127 410 252 238
Cuts at: 1670 1769 1816 1839 2008 2058 2110 2209 2383
Size: 99 47 23 169 50 52 99 174
Cuts at: 2383 2766 2817
Size: 383 51
Fragments arranged by size:
410 383 331 252 238 174 169 165
127 99 99 82 65 52 51 50
47 23
BspEI T'CCGG_A
Cuts at: 0 377 2817
Size: 377 2440
BspHI T'CATG_A
Cuts at: 0 1022 2252 2387 2817
Size: 1022 1230 135 430
Fragments arranged by size:
1230 1022 430 135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 593 1503 1562 1889 2231 2238 2817
Size: 593 910 59 327 342 7 579
Fragments arranged by size:
910 593 579 342 327 59 7
BsrBI CCG'CTC
Cuts at: 0 726 1925 2817
Size: 726 1199 892
Fragments arranged by size:
1199 892 726
BsrFI r'CCGG_y
Cuts at: 0 2504 2817
Size: 2504 313
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2593 2817
Size: 2593 224
BstEII G'GTnAC_C
Cuts at: 0 729 1888 2817
Size: 729 1159 929
Fragments arranged by size:
1159 929 729
BstXI CCAn_nnnn'nTGG
Cuts at: 0 528 540 1149 1617 2016 2103 2217 2817
Size: 528 12 609 468 399 87 114 600
Fragments arranged by size:
609 600 528 468 399 114 87 12
BstYI r'GATC_y
Cuts at: 0 41 399 656 944 2177 2354 2717 2817
Size: 41 358 257 288 1233 177 363 100
Fragments arranged by size:
1233 363 358 288 257 177 100 41
BstZ17I GTA'TAC
Cuts at: 0 207 2817
Size: 207 2610
Bsu36I CC'TnA_GG
Cuts at: 0 1325 2817
Size: 1325 1492
BtgI C'CryG_G
Cuts at: 0 125 326 521 1563 1592 2216 2817
Size: 125 201 195 1042 29 624 601
Fragments arranged by size:
1042 624 601 201 195 125 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1035 2817
Size: 1035 1782
BtsI GCAGTG_nn'
Cuts at: 0 104 323 545 835 868 2073 2467 2817
Size: 104 219 222 290 33 1205 394 350
Fragments arranged by size:
1205 394 350 290 222 219 104 33
ClaI AT'CG_AT
Cuts at: 0 118 2438 2817
Size: 118 2320 379
Fragments arranged by size:
2320 379 118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1073 1108 2817
Size: 1073 35 1709
Fragments arranged by size:
1709 1073 35
DraIII CAC_nnn'GTG
Cuts at: 0 647 1295 1297 2817
Size: 647 648 2 1520
Fragments arranged by size:
1520 648 647 2
EaeI y'GGCC_r
Cuts at: 0 248 257 434 530 1131 1526 1937 2420
Size: 248 9 177 96 601 395 411 483
Cuts at: 2420 2519 2817
Size: 99 298
Fragments arranged by size:
601 483 411 395 298 248 177 99
96 9
EarI CTCTTCn'nnn_
Cuts at: 0 154 1643 2817
Size: 154 1489 1174
Fragments arranged by size:
1489 1174 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1018 2722 2817
Size: 1018 1704 95
Fragments arranged by size:
1704 1018 95
EcoICRI GAG'CTC
Cuts at: 0 63 2817
Size: 63 2754
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 208 214 409 970 1027 1417 1667
Size: 73 135 6 195 561 57 390 250
Cuts at: 1667 1853 2194 2299 2398 2436 2698 2773 2817
Size: 186 341 105 99 38 262 75 44
Fragments arranged by size:
561 390 341 262 250 195 186 135
105 99 75 73 57 44 38 6
EcoO109I rG'GnC_Cy
Cuts at: 0 288 1515 2817
Size: 288 1227 1302
Fragments arranged by size:
1302 1227 288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 987 1266 2817
Size: 987 279 1551
Fragments arranged by size:
1551 987 279
FspI TGC'GCA
Cuts at: 0 412 910 2817
Size: 412 498 1907
Fragments arranged by size:
1907 498 412
HaeII r_GCGC'y
Cuts at: 0 550 1170 1584 2817
Size: 550 620 414 1233
Fragments arranged by size:
1233 620 550 414
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 793 825 2045 2077 2385 2417
Size: 490 32 271 32 1220 32 308 32
Cuts at: 2417 2817
Size: 400
Fragments arranged by size:
1220 490 400 308 271 32 32 32
32
HincII GTy'rAC
Cuts at: 0 165 264 345 1209 2817
Size: 165 99 81 864 1608
Fragments arranged by size:
1608 864 165 99 81
KasI G'GCGC_C
Cuts at: 0 1580 2817
Size: 1580 1237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 2050 2817
Size: 533 1517 767
Fragments arranged by size:
1517 767 533
MscI TGG'CCA
Cuts at: 0 250 436 532 1528 1939 2521 2817
Size: 250 186 96 996 411 582 296
Fragments arranged by size:
996 582 411 296 250 186 96
MslI CAynn'nnrTG
Cuts at: 0 271 526 802 1147 2215 2392 2698 2817
Size: 271 255 276 345 1068 177 306 119
Fragments arranged by size:
1068 345 306 276 271 255 177 119
MspA1I CmG'CkG
Cuts at: 0 424 462 760 1136 1443 1449 2229 2738
Size: 424 38 298 376 307 6 780 509
Cuts at: 2738 2817
Size: 79
Fragments arranged by size:
780 509 424 376 307 298 79 38
6
NarI GG'CG_CC
Cuts at: 0 1581 2817
Size: 1581 1236
NcoI C'CATG_G
Cuts at: 0 326 521 2216 2817
Size: 326 195 1695 601
Fragments arranged by size:
1695 601 326 195
NdeI CA'TA_TG
Cuts at: 0 405 2124 2817
Size: 405 1719 693
Fragments arranged by size:
1719 693 405
NspI r_CATG'y
Cuts at: 0 174 2664 2817
Size: 174 2490 153
Fragments arranged by size:
2490 174 153
PasI CC'CwG_GG
Cuts at: 0 1872 2817
Size: 1872 945
PflMI CCAn_nnn'nTGG
Cuts at: 0 465 1062 1346 1503 2349 2817
Size: 465 597 284 157 846 468
Fragments arranged by size:
846 597 468 465 284 157
PfoI T'CCnGG_A
Cuts at: 0 482 665 1220 2817
Size: 482 183 555 1597
Fragments arranged by size:
1597 555 482 183
PmlI CAC'GTG
Cuts at: 0 2784 2817
Size: 2784 33
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2542 2574 2817
Size: 2542 32 243
Fragments arranged by size:
2542 243 32
PpuMI rG'GwC_Cy
Cuts at: 0 288 1515 2817
Size: 288 1227 1302
Fragments arranged by size:
1302 1227 288
PshAI GACnn'nnGTC
Cuts at: 0 1204 2817
Size: 1204 1613
PspXI vC'TCGA_Gb
Cuts at: 0 1408 1794 2817
Size: 1408 386 1023
Fragments arranged by size:
1408 1023 386
PstI C_TGCA'G
Cuts at: 0 896 1448 2228 2612 2817
Size: 896 552 780 384 205
Fragments arranged by size:
896 780 552 384 205
PvuII CAG'CTG
Cuts at: 0 424 462 1443 1449 2229 2738 2817
Size: 424 38 981 6 780 509 79
Fragments arranged by size:
981 780 509 424 79 38 6
SacI G_AGCT'C
Cuts at: 0 65 2817
Size: 65 2752
SfcI C'TryA_G
Cuts at: 0 892 1444 2224 2608 2817
Size: 892 552 780 384 209
Fragments arranged by size:
892 780 552 384 209
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 256 1042 2817
Size: 256 786 1775
Fragments arranged by size:
1775 786 256
SfoI GGC'GCC
Cuts at: 0 1582 2817
Size: 1582 1235
SmaI CCC'GGG
Cuts at: 0 1333 2817
Size: 1333 1484
SmlI C'TyrA_G
Cuts at: 0 841 1408 1794 2809 2817
Size: 841 567 386 1015 8
Fragments arranged by size:
1015 841 567 386 8
SphI G_CATG'C
Cuts at: 0 174 2817
Size: 174 2643
SrfI GCCC'GGGC
Cuts at: 0 1333 2817
Size: 1333 1484
SspI AAT'ATT
Cuts at: 0 16 2817
Size: 16 2801
StuI AGG'CCT
Cuts at: 0 939 2242 2794 2817
Size: 939 1303 552 23
Fragments arranged by size:
1303 939 552 23
StyI C'CwwG_G
Cuts at: 0 326 521 692 1202 1610 2216 2817
Size: 326 195 171 510 408 606 601
Fragments arranged by size:
606 601 510 408 326 195 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1876 2073 2272 2373 2688 2817
Size: 1876 197 199 101 315 129
Fragments arranged by size:
1876 315 199 197 129 101
TatI w'GTAC_w
Cuts at: 0 980 2817
Size: 980 1837
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2111 2143 2459 2491 2817
Size: 2111 32 316 32 326
Fragments arranged by size:
2111 326 316 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 759 1599 1740 2346 2643 2817
Size: 759 840 141 606 297 174
Fragments arranged by size:
840 759 606 297 174 141
XhoI C'TCGA_G
Cuts at: 0 1408 1794 2817
Size: 1408 386 1023
Fragments arranged by size:
1408 1023 386
XmaI C'CCGG_G
Cuts at: 0 1331 2817
Size: 1331 1486
XmnI GAAnn'nnTTC
Cuts at: 0 967 2817
Size: 967 1850
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AloI AlwNI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BcgI
BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NcoI NdeI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PshAI
PspXI PstI PvuII SacI SfcI SfiI SfoI SmaI
SmlI SphI SrfI SspI StuI StyI TaqII TatI
TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BbvCI BciVI
BclI BfrBI BlpI BmgBI BmtI BplI BsiWI BsmI
BsrDI BsrGI BssHII BssSI BstBI DraI DrdI EagI
EcoNI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PsiI PspOMI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SgfI
SgrAI SnaBI SpeI SwaI Tth111I XbaI ZraI