(Circular) (Six-base) MAPSORT of: pFN21AB4095.seq Check: 2722 from: 1 to: 7954 pFN21AB4095 7954 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4217 4217 Size: 7954 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5764 278 Size: 53 83 186 936 4228 2468 Fragments arranged by size: 4228 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2082 5297 1937 Size: 145 3215 4594 Fragments arranged by size: 4594 3215 145 Acc65I G'GTAC_C Cuts at: 5275 5275 Size: 7954 AclI AA'CG_TT Cuts at: 1563 6081 6454 1563 Size: 4518 373 3063 Fragments arranged by size: 4518 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2927 3545 5060 6017 7401 7669 1632 Size: 1295 618 1515 957 1384 268 1917 Fragments arranged by size: 1917 1515 1384 1295 957 618 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7757 AflIII A'CryG_T Cuts at: 1203 3369 3678 6853 7830 1203 Size: 2166 309 3175 977 1327 Fragments arranged by size: 3175 2166 1327 977 309 AgeI A'CCGG_T Cuts at: 6785 6785 Size: 7954 AhdI GACnn_n'nnGTC Cuts at: 4588 6683 4588 Size: 2095 5859 AleI CACnn'nnGTG Cuts at: 1166 4017 4247 1166 Size: 2851 230 4873 Fragments arranged by size: 4873 2851 230 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4025 4035 1184 Size: 2841 10 5103 Fragments arranged by size: 5103 2841 10 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2885 2917 4543 4575 2885 Size: 32 1626 32 6264 Fragments arranged by size: 6264 1626 32 32 AlwNI CAG_nnn'CTG Cuts at: 2500 3007 3673 4926 5697 7269 2500 Size: 507 666 1253 771 1572 3185 Fragments arranged by size: 3185 1572 1253 771 666 507 ApaI G_GGCC'C Cuts at: 4140 4140 Size: 7954 ApaLI G'TGCA_C Cuts at: 6011 7167 6011 Size: 1156 6798 ApoI r'AATT_y Cuts at: 1493 3484 5263 5489 5641 7750 1493 Size: 1991 1779 226 152 2109 1697 Fragments arranged by size: 2109 1991 1779 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6508 160 Size: 872 5476 1606 Fragments arranged by size: 5476 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7954 AvaI C'yCGr_G Cuts at: 1870 1943 3537 4264 4825 5279 1870 Size: 73 1594 727 561 454 4545 Fragments arranged by size: 4545 1594 727 561 454 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4965 4998 4965 Size: 33 7921 BamHI G'GATC_C Cuts at: 2518 3619 5284 5651 2518 Size: 1101 1665 367 4821 Fragments arranged by size: 4821 1665 1101 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2641 2755 4260 4735 5275 Size: 325 217 627 854 114 1505 475 540 Cuts at: 5275 6730 618 Size: 1455 1842 Fragments arranged by size: 1842 1505 1455 854 627 540 475 325 217 114 BanII G_rGCy'C Cuts at: 1391 2359 2475 2886 3291 3938 4140 4256 4524 Size: 968 116 411 405 647 202 116 268 Cuts at: 4524 4832 4864 5273 1391 Size: 308 32 409 4072 Fragments arranged by size: 4072 968 647 411 409 405 308 268 202 116 116 32 BbeI G_GCGC'C Cuts at: 4264 4739 4264 Size: 475 7479 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6983 BbvCI CC'TCA_GC Cuts at: 3314 4488 3314 Size: 1174 6780 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6145 6179 1903 Size: 34 4208 34 3678 Fragments arranged by size: 4208 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4966 5846 7056 4966 Size: 880 1210 5864 Fragments arranged by size: 5864 1210 880 BclI T'GATC_A Cuts at: 1548 3135 1548 Size: 1587 6367 BfrBI ATG'CAT Cuts at: 3517 3517 Size: 7954 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2223 3099 4056 Size: 107 122 71 631 605 551 876 957 Cuts at: 4056 4269 4749 4811 4836 6565 136 Size: 213 480 62 25 1729 1525 Fragments arranged by size: 1729 1525 957 876 631 605 551 480 213 122 107 71 62 25 BglII A'GATC_T Cuts at: 7949 7949 Size: 7954 BlpI GC'TnA_GC Cuts at: 5371 5371 Size: 7954 Bme1580I G_kGCm'C Cuts at: 1790 2760 4140 6015 7171 1790 Size: 970 1380 1875 1156 2573 Fragments arranged by size: 2573 1875 1380 1156 970 BmgBI CAC'GTC Cuts at: 1318 2779 1318 Size: 1461 6493 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2411 4011 4936 Size: 416 53 83 399 24 994 1600 925 Cuts at: 4936 5685 6643 442 Size: 749 958 1753 Fragments arranged by size: 1753 1600 994 958 925 749 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4818 1056 Size: 3762 4192 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2329 4219 4933 2329 Size: 1890 714 5350 Fragments arranged by size: 5350 1890 714 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2398 3227 4044 4355 4878 4903 5102 6614 Size: 1159 829 817 311 523 25 199 1512 Cuts at: 6614 1239 Size: 2579 Fragments arranged by size: 2579 1512 1159 829 817 523 311 199 25 Bpu10I CC'TnA_GC Cuts at: 2865 3314 4488 4900 2865 Size: 449 1174 412 5919 Fragments arranged by size: 5919 1174 449 412 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2819 3347 5195 6079 6944 7242 2819 Size: 528 1848 884 865 298 3531 Fragments arranged by size: 3531 1848 884 865 528 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3653 6617 882 Size: 617 2154 2964 2219 Fragments arranged by size: 2964 2219 2154 617 BsaAI yAC'GTr Cuts at: 493 1204 2671 2695 2878 3424 5745 493 Size: 711 1467 24 183 546 2321 2702 Fragments arranged by size: 2702 2321 1467 711 546 183 24 BsaBI GATnn'nnATC Cuts at: 2343 2523 3338 7948 2343 Size: 180 815 4610 2349 Fragments arranged by size: 4610 2349 815 180 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4261 4295 4316 4409 Size: 53 83 186 936 2728 34 21 93 Cuts at: 4409 4625 4634 4736 5151 5761 6143 6778 275 Size: 216 9 102 415 610 382 635 1451 Fragments arranged by size: 2728 1451 936 635 610 415 382 216 186 102 93 83 53 34 21 9 BsaWI w'CCGG_w Cuts at: 6387 6785 7059 7206 7617 6387 Size: 398 274 147 411 6724 Fragments arranged by size: 6724 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2436 2466 2885 2915 1178 Size: 30 165 30 1033 30 419 30 6217 Fragments arranged by size: 6217 1033 419 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2054 2084 2156 2159 2168 2171 2192 Size: 865 3 30 72 3 9 3 21 Cuts at: 2192 3494 1186 Size: 1302 5646 Fragments arranged by size: 5646 1302 865 72 30 21 9 3 3 3 BseYI C'CCAG_C Cuts at: 2490 2885 3382 3929 4703 4709 4744 4771 4820 Size: 395 497 547 774 6 35 27 49 Cuts at: 4820 7157 7653 2490 Size: 2337 496 2791 Fragments arranged by size: 2791 2337 774 547 497 496 395 49 35 27 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2121 3632 4184 1163 Size: 573 385 1511 552 4933 Fragments arranged by size: 4933 1511 573 552 385 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2935 5404 6165 7193 1540 Size: 275 186 934 2469 761 1028 2301 Fragments arranged by size: 2469 2301 1028 934 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2475 2784 3227 3246 3291 3534 4864 5030 5079 Size: 309 443 19 45 243 1330 166 49 Cuts at: 5079 6015 6100 7171 2475 Size: 936 85 1071 3258 Fragments arranged by size: 3258 1330 1071 936 443 309 243 166 85 49 45 19 BsmI GAATG_Cn' Cuts at: 5465 5558 5465 Size: 93 7861 BsmBI CGTCTCn'nnnn_ Cuts at: 2441 2441 Size: 7954 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2359 2475 2760 2784 2886 3227 3246 Size: 399 569 116 285 24 102 341 19 Cuts at: 3246 3291 3534 3938 4140 4256 4524 4832 4864 Size: 45 243 404 202 116 268 308 32 Cuts at: 4864 5030 5079 5273 6015 6100 7171 1391 Size: 166 49 194 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 569 404 399 341 308 285 268 243 202 194 166 116 116 102 85 49 45 32 24 19 BspEI T'CCGG_A Cuts at: 7617 7617 Size: 7954 BspHI T'CATG_A Cuts at: 5843 7573 5843 Size: 1730 6224 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2733 3306 3552 4217 5309 844 Size: 1889 573 246 665 1092 3489 Fragments arranged by size: 3489 1889 1092 665 573 246 BsrBI CCG'CTC Cuts at: 1394 1823 2092 3238 5841 1394 Size: 429 269 1146 2603 3507 Fragments arranged by size: 3507 2603 1146 429 269 BsrDI GCAATG_nn' Cuts at: 65 1248 2592 2859 3865 6449 6623 65 Size: 1183 1344 267 1006 2584 174 1396 Fragments arranged by size: 2584 1396 1344 1183 1006 267 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4636 6598 6785 1700 Size: 110 2826 1962 187 2869 Fragments arranged by size: 2869 2826 1962 187 110 BsrGI T'GTAC_A Cuts at: 96 4644 96 Size: 4548 3406 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7954 BssSI C'ACGA_G Cuts at: 6014 7026 6014 Size: 1012 6942 BstAPI GCAn_nnn'nTGC Cuts at: 794 2500 2755 794 Size: 1706 255 5993 Fragments arranged by size: 5993 1706 255 BstBI TT'CG_AA Cuts at: 6758 6758 Size: 7954 BstEII G'GTnAC_C Cuts at: 1184 4930 1184 Size: 3746 4208 BstXI CCAn_nnnn'nTGG Cuts at: 2823 4225 4571 2823 Size: 1402 346 6206 Fragments arranged by size: 6206 1402 346 BstYI r'GATC_y Cuts at: 1972 2462 2518 3309 3619 5214 5284 5651 6036 Size: 490 56 791 310 1595 70 367 385 Cuts at: 6036 6053 7505 7591 7603 7949 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1595 1452 791 490 385 367 346 310 86 70 56 17 12 Bsu36I CC'TnA_GG Cuts at: 2130 2130 Size: 7954 BtgI C'CryG_G Cuts at: 513 1065 2218 2565 3966 4182 4245 4800 5145 Size: 552 1153 347 1401 216 63 555 345 Cuts at: 5145 513 Size: 3322 Fragments arranged by size: 3322 1401 1153 555 552 347 345 216 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7215 Fragments arranged by size: 7215 683 56 BtsI GCAGTG_nn' Cuts at: 2065 2116 4069 5014 5470 6264 6284 2065 Size: 51 1953 945 456 794 20 3735 Fragments arranged by size: 3735 1953 945 794 456 51 20 ClaI AT'CG_AT Cuts at: 1554 2322 1554 Size: 768 7186 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7919 DraI TTT'AAA Cuts at: 5258 5258 Size: 7954 DraIII CAC_nnn'GTG Cuts at: 3922 4732 3922 Size: 810 7144 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6961 809 Size: 507 5645 1802 Fragments arranged by size: 5645 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2568 2802 3439 Size: 54 1425 50 275 18 738 234 637 Cuts at: 3439 4111 4638 5401 6290 7919 8 Size: 672 527 763 889 1629 43 Fragments arranged by size: 1629 1425 889 763 738 672 637 527 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5401 1537 Size: 275 3589 4090 Fragments arranged by size: 4090 3589 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 2118 2133 2145 2169 3848 5884 1353 Size: 729 36 15 12 24 1679 2036 3423 Fragments arranged by size: 3423 2036 1679 729 36 24 15 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2110 2819 6541 6915 7061 387 Size: 1064 138 521 709 3722 374 146 1280 Fragments arranged by size: 3722 1280 1064 709 521 374 146 138 EcoICRI GAG'CTC Cuts at: 2473 3289 4862 2473 Size: 816 1573 5565 Fragments arranged by size: 5565 1573 816 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2398 2927 3227 3545 4044 4355 4878 Size: 393 766 529 300 318 499 311 523 Cuts at: 4878 4903 5060 5102 6017 6614 7401 7669 1239 Size: 25 157 42 915 597 787 268 1524 Fragments arranged by size: 1524 915 787 766 597 529 523 499 393 318 311 300 268 157 42 25 EcoNI CCTnn'n_nnAGG Cuts at: 4288 5129 4288 Size: 841 7113 EcoO109I rG'GnC_Cy Cuts at: 3030 3358 3541 4136 4137 4449 4456 3030 Size: 328 183 595 1 312 7 6528 Fragments arranged by size: 6528 595 328 312 183 7 1 EcoRI G'AATT_C Cuts at: 5263 5641 5263 Size: 378 7576 FspI TGC'GCA Cuts at: 2585 4124 6460 2585 Size: 1539 2336 4079 Fragments arranged by size: 4079 2336 1539 FspAI rTGC'GCAy Cuts at: 4124 4124 Size: 7954 HaeII r_GCGC'y Cuts at: 1678 4264 4739 7101 1678 Size: 2586 475 2362 2531 Fragments arranged by size: 2586 2531 2362 475 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2459 2491 3035 3067 3551 Size: 32 135 32 887 32 544 32 484 Cuts at: 3551 3583 3823 3855 4574 4606 4646 4678 6595 Size: 32 240 32 719 32 40 32 1917 Cuts at: 6595 6627 6669 6701 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1917 887 719 544 484 240 135 42 40 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2251 4603 5298 5544 669 Size: 988 281 313 2352 695 246 3079 Fragments arranged by size: 3079 2352 988 695 313 281 246 HindIII A'AGCT_T Cuts at: 748 3850 748 Size: 3102 4852 HpaI GTT'AAC Cuts at: 5544 5544 Size: 7954 KasI G'GCGC_C Cuts at: 4260 4735 4260 Size: 475 7479 KpnI G_GTAC'C Cuts at: 5279 5279 Size: 7954 MfeI C'AATT_G Cuts at: 5553 5553 Size: 7954 MluI A'CGCG_T Cuts at: 7830 7830 Size: 7954 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 3277 3354 7068 7252 1305 Size: 684 1288 77 3714 184 2007 Fragments arranged by size: 3714 2007 1288 684 184 77 MscI TGG'CCA Cuts at: 10 64 1489 1832 2804 10 Size: 54 1425 343 972 5160 Fragments arranged by size: 5160 1425 972 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2669 3341 3776 4017 4157 4223 4247 Size: 648 1503 672 435 241 140 66 24 Cuts at: 4247 4946 5912 6271 6430 518 Size: 699 966 359 159 2042 Fragments arranged by size: 2042 1503 966 699 672 648 435 359 241 159 140 66 24 MspA1I CmG'CkG Cuts at: 1606 2105 2500 3616 4237 5370 6047 7195 7440 Size: 499 395 1116 621 1133 677 1148 245 Cuts at: 7440 1606 Size: 2120 Fragments arranged by size: 2120 1148 1133 1116 677 621 499 395 245 NaeI GCC'GGC Cuts at: 4638 4638 Size: 7954 NarI GG'CG_CC Cuts at: 4261 4736 4261 Size: 475 7479 NcoI C'CATG_G Cuts at: 513 1065 2218 4800 513 Size: 552 1153 2582 3667 Fragments arranged by size: 3667 2582 1153 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7954 NgoMIV G'CCGG_C Cuts at: 4636 4636 Size: 7954 NheI G'CTAG_C Cuts at: 1052 4814 1052 Size: 3762 4192 NotI GC'GGCC_GC Cuts at: 5401 5401 Size: 7954 NsiI A_TGCA'T Cuts at: 3519 3519 Size: 7954 NspI r_CATG'y Cuts at: 1132 1229 2654 3373 3682 3898 5026 5312 6857 Size: 97 1425 719 309 216 1128 286 1545 Cuts at: 6857 1132 Size: 2229 Fragments arranged by size: 2229 1545 1425 1128 719 309 286 216 97 PasI CC'CwG_GG Cuts at: 1348 3034 4132 1348 Size: 1686 1098 5170 Fragments arranged by size: 5170 1686 1098 PciI A'CATG_T Cuts at: 3369 3678 6853 3369 Size: 309 3175 4470 Fragments arranged by size: 4470 3175 309 PflMI CCAn_nnn'nTGG Cuts at: 1108 2529 2752 3631 4732 4926 1108 Size: 1421 223 879 1101 194 4136 Fragments arranged by size: 4136 1421 1101 879 223 194 PfoI T'CCnGG_A Cuts at: 2334 4371 5244 2334 Size: 2037 873 5044 Fragments arranged by size: 5044 2037 873 PmeI GTTT'AAAC Cuts at: 5258 5258 Size: 7954 PmlI CAC'GTG Cuts at: 2695 3424 2695 Size: 729 7225 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2885 2917 6023 6055 7536 7568 2885 Size: 32 3106 32 1481 32 3271 Fragments arranged by size: 3271 3106 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3030 3358 3541 4456 3030 Size: 328 183 915 6528 Fragments arranged by size: 6528 915 328 183 PshAI GACnn'nnGTC Cuts at: 1613 4997 1613 Size: 3384 4570 PsiI TTA'TAA Cuts at: 5524 5524 Size: 7954 PspOMI G'GGCC_C Cuts at: 4136 4136 Size: 7954 PstI C_TGCA'G Cuts at: 830 2625 3303 3549 5306 830 Size: 1795 678 246 1757 3478 Fragments arranged by size: 3478 1795 1757 678 246 PvuI CG_AT'CG Cuts at: 2001 2935 2001 Size: 934 7020 PvuII CAG'CTG Cuts at: 2500 3616 2500 Size: 1116 6838 SacI G_AGCT'C Cuts at: 2475 3291 4864 2475 Size: 816 1573 5565 Fragments arranged by size: 5565 1573 816 SalI G'TCGA_C Cuts at: 1936 5296 1936 Size: 3360 4594 SanDI GG'GwC_CC Cuts at: 4456 4456 Size: 7954 SapI GCTCTTCn'nnn_ Cuts at: 2082 2118 2082 Size: 36 7918 SbfI CC_TGCA'GG Cuts at: 3303 5306 3303 Size: 2003 5951 ScaI AGT'ACT Cuts at: 6202 7914 6202 Size: 1712 6242 SexAI A'CCwGG_T Cuts at: 3918 4728 3918 Size: 810 7144 SfcI C'TryA_G Cuts at: 826 1045 2621 3299 3545 4840 5302 6437 7118 Size: 219 1576 678 246 1295 462 1135 681 Cuts at: 7118 7309 826 Size: 191 1471 Fragments arranged by size: 1576 1471 1295 1135 681 678 462 246 219 191 SfoI GGC'GCC Cuts at: 4262 4737 4262 Size: 475 7479 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7954 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7954 SmaI CCC'GGG Cuts at: 1872 3539 4266 5281 1872 Size: 1667 727 1015 4545 Fragments arranged by size: 4545 1667 1015 727 SmlI C'TyrA_G Cuts at: 820 1017 1943 2834 3362 5210 6058 6959 7221 Size: 197 926 891 528 1848 848 901 262 Cuts at: 7221 820 Size: 1553 Fragments arranged by size: 1848 1553 926 901 891 848 528 262 197 SnaBI TAC'GTA Cuts at: 493 5745 493 Size: 5252 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7954 SphI G_CATG'C Cuts at: 1132 5312 1132 Size: 4180 3774 SrfI GCCC'GGGC Cuts at: 4266 4266 Size: 7954 SspI AAT'ATT Cuts at: 5 52 5878 5 Size: 47 5826 2081 Fragments arranged by size: 5826 2081 47 StuI AGG'CCT Cuts at: 1517 2678 3462 4541 1517 Size: 1161 784 1079 4930 Fragments arranged by size: 4930 1161 1079 784 StyI C'CwwG_G Cuts at: 513 1065 2218 3750 4800 5393 513 Size: 552 1153 1532 1050 593 3074 Fragments arranged by size: 3074 1532 1153 1050 593 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2211 2504 3623 3959 4360 5981 5998 Size: 1255 631 293 1119 336 401 1621 17 Cuts at: 5998 6151 6336 6781 325 Size: 153 185 445 1498 Fragments arranged by size: 1621 1498 1255 1119 631 445 401 336 293 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4644 6200 7912 Size: 274 80 33 51 1443 2667 1556 1712 Cuts at: 7912 96 Size: 138 Fragments arranged by size: 2667 1712 1556 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2436 2468 7914 7946 2436 Size: 32 5446 32 2444 Fragments arranged by size: 5446 2444 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7954 XbaI T'CTAG_A Cuts at: 2302 5290 2302 Size: 2988 4966 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2282 2437 2713 3964 4189 5077 2282 Size: 155 276 1251 225 888 5159 Fragments arranged by size: 5159 1251 888 276 225 155 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7954 XmaI C'CCGG_G Cuts at: 1870 3537 4264 5279 1870 Size: 1667 727 1015 4545 Fragments arranged by size: 4545 1667 1015 727 XmnI GAAnn'nnTTC Cuts at: 6083 6083 Size: 7954 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5762 276 Size: 53 83 186 936 4228 2468 Fragments arranged by size: 4228 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BsiWI BstZ17I EcoRV FalI FseI NruI PacI PspXI PsrI RsrII SacII SfiI SwaI