(Circular) (Six-base) MAPSORT of: pFN21AB3990.seq Check: 4465 from: 1 to: 7879 pFN21AB3990 7879 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3649 4059 4196 3649 Size: 410 137 7332 Fragments arranged by size: 7332 410 137 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5689 278 Size: 53 83 186 936 4153 2468 Fragments arranged by size: 4153 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5222 1937 Size: 3285 4594 Acc65I G'GTAC_C Cuts at: 3790 5200 3790 Size: 1410 6469 AclI AA'CG_TT Cuts at: 1563 6006 6379 1563 Size: 4443 373 3063 Fragments arranged by size: 4443 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3590 3803 4814 4940 5942 7326 7594 1632 Size: 1958 213 1011 126 1002 1384 268 1917 Fragments arranged by size: 1958 1917 1384 1011 1002 268 213 126 AflII C'TTAA_G Cuts at: 820 1017 2263 820 Size: 197 1246 6436 Fragments arranged by size: 6436 1246 197 AflIII A'CryG_T Cuts at: 1203 2709 3062 6778 7755 1203 Size: 1506 353 3716 977 1327 Fragments arranged by size: 3716 1506 1327 977 353 AgeI A'CCGG_T Cuts at: 6710 6710 Size: 7879 AhdI GACnn_n'nnGTC Cuts at: 6608 6608 Size: 7879 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7879 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7879 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3929 3961 3972 4004 3929 Size: 32 11 32 7804 Fragments arranged by size: 7804 32 32 11 AlwNI CAG_nnn'CTG Cuts at: 2632 3441 3586 4291 5622 7194 2632 Size: 809 145 705 1331 1572 3317 Fragments arranged by size: 3317 1572 1331 809 705 145 ApaI G_GGCC'C Cuts at: 2803 3475 2803 Size: 672 7207 ApaLI G'TGCA_C Cuts at: 2726 5936 7092 2726 Size: 3210 1156 3513 Fragments arranged by size: 3513 3210 1156 ApoI r'AATT_y Cuts at: 1493 5077 5188 5414 5566 7675 1493 Size: 3584 111 226 152 2109 1697 Fragments arranged by size: 3584 2109 1697 226 152 111 AseI AT'TA_AT Cuts at: 160 1032 6433 160 Size: 872 5401 1606 Fragments arranged by size: 5401 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7879 AvaI C'yCGr_G Cuts at: 1870 1943 2759 4039 4600 5204 1870 Size: 73 816 1280 561 604 4545 Fragments arranged by size: 4545 1280 816 604 561 73 BamHI G'GATC_C Cuts at: 2895 5209 5576 2895 Size: 2314 367 5198 Fragments arranged by size: 5198 2314 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2746 2840 3011 3125 3790 Size: 325 217 627 959 94 171 114 665 Cuts at: 3790 4072 5200 6655 618 Size: 282 1128 1455 1842 Fragments arranged by size: 1842 1455 1128 959 665 627 325 282 217 171 114 94 BanII G_rGCy'C Cuts at: 1391 2364 2803 2937 3102 3251 3324 3433 3475 Size: 973 439 134 165 149 73 109 42 Cuts at: 3475 4135 5198 1391 Size: 660 1063 4072 Fragments arranged by size: 4072 1063 973 660 439 165 149 134 109 73 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4104 928 Size: 971 2205 4703 Fragments arranged by size: 4703 2205 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6070 6104 1903 Size: 34 4133 34 3678 Fragments arranged by size: 4133 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5771 6981 5771 Size: 1210 6669 BclI T'GATC_A Cuts at: 1548 4842 1548 Size: 3294 4585 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3074 4060 4300 Size: 107 122 71 631 605 1402 986 240 Cuts at: 4300 4715 6490 136 Size: 415 1775 1525 Fragments arranged by size: 1775 1525 1402 986 631 605 415 240 122 107 71 BglII A'GATC_T Cuts at: 7874 7874 Size: 7879 BlpI GC'TnA_GC Cuts at: 3318 4268 5296 3318 Size: 950 1028 5901 Fragments arranged by size: 5901 1028 950 Bme1580I G_kGCm'C Cuts at: 1790 2353 2730 2751 2803 2828 2890 3070 3475 Size: 563 377 21 52 25 62 180 405 Cuts at: 3475 5940 7096 1790 Size: 2465 1156 2573 Fragments arranged by size: 2573 2465 1156 563 405 377 180 62 52 25 21 BmgBI CAC'GTC Cuts at: 1318 3063 4102 4915 1318 Size: 1745 1039 813 4282 Fragments arranged by size: 4282 1745 1039 813 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5610 6568 442 Size: 416 53 83 399 24 4193 958 1753 Fragments arranged by size: 4193 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7879 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4699 4699 Size: 7879 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2195 2522 2946 3159 3620 4166 4561 4682 Size: 956 327 424 213 461 546 395 121 Cuts at: 4682 4805 6539 1239 Size: 123 1734 2579 Fragments arranged by size: 2579 1734 956 546 461 424 395 327 213 123 121 Bpu10I CC'TnA_GC Cuts at: 4056 5132 4056 Size: 1076 6803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2091 2190 3434 3632 3843 6004 6869 7167 2091 Size: 99 1244 198 211 2161 865 298 2803 Fragments arranged by size: 2803 2161 1244 865 298 211 198 99 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4475 6542 882 Size: 617 2976 2067 2219 Fragments arranged by size: 2976 2219 2067 617 BsaAI yAC'GTr Cuts at: 493 1204 2878 5670 493 Size: 711 1674 2792 2702 Fragments arranged by size: 2792 2702 1674 711 BsaBI GATnn'nnATC Cuts at: 3897 7873 3897 Size: 3976 3903 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2646 3231 4887 5686 Size: 53 83 186 936 1113 585 1656 799 Cuts at: 5686 6068 6703 275 Size: 382 635 1451 Fragments arranged by size: 1656 1451 1113 936 799 635 585 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5088 6312 6710 6984 7131 7542 5088 Size: 1224 398 274 147 411 5425 Fragments arranged by size: 5425 1224 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3972 4002 4427 4457 1178 Size: 30 165 30 2569 30 425 30 4600 Fragments arranged by size: 4600 2569 425 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2498 3557 3902 4163 4451 4454 4510 Size: 837 475 1059 345 261 288 3 56 Cuts at: 4510 4564 1186 Size: 54 4501 Fragments arranged by size: 4501 1059 837 475 345 288 261 56 54 3 BseYI C'CCAG_C Cuts at: 2318 2868 4339 7082 7578 2318 Size: 550 1471 2743 496 2619 Fragments arranged by size: 2743 2619 1471 550 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3166 4834 1163 Size: 573 1430 1668 4208 Fragments arranged by size: 4208 1668 1430 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2445 2866 3342 5329 6090 7118 Size: 275 186 444 421 476 1987 761 1028 Cuts at: 7118 1540 Size: 2301 Fragments arranged by size: 2301 1987 1028 761 476 444 421 275 186 BsiHKAI G_wGCw'C Cuts at: 2730 3251 4415 4935 5940 6025 7096 2730 Size: 521 1164 520 1005 85 1071 3513 Fragments arranged by size: 3513 1164 1071 1005 521 520 85 BsmI GAATG_Cn' Cuts at: 3050 5390 5483 3050 Size: 2340 93 5446 Fragments arranged by size: 5446 2340 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2353 2364 2730 2751 2803 2828 2890 Size: 399 563 11 366 21 52 25 62 Cuts at: 2890 2937 3070 3102 3251 3324 3433 3475 4135 Size: 47 133 32 149 73 109 42 660 Cuts at: 4135 4415 4935 5198 5940 6025 7096 1391 Size: 280 520 263 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 660 563 520 399 366 280 263 149 133 109 85 73 62 52 47 42 32 25 21 11 BspEI T'CCGG_A Cuts at: 5088 7542 5088 Size: 2454 5425 BspHI T'CATG_A Cuts at: 5768 7498 5768 Size: 1730 6149 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3649 3664 4059 4196 4457 5234 844 Size: 2805 15 395 137 261 777 3489 Fragments arranged by size: 3489 2805 777 395 261 137 15 BsrBI CCG'CTC Cuts at: 1394 1823 2248 4060 4591 5766 1394 Size: 429 425 1812 531 1175 3507 Fragments arranged by size: 3507 1812 1175 531 429 425 BsrDI GCAATG_nn' Cuts at: 65 1248 6374 6548 65 Size: 1183 5126 174 1396 Fragments arranged by size: 5126 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2335 4636 6523 6710 1700 Size: 110 525 2301 1887 187 2869 Fragments arranged by size: 2869 2301 1887 525 187 110 BsrGI T'GTAC_A Cuts at: 96 3084 96 Size: 2988 4891 BssHII G'CGCG_C Cuts at: 1925 3604 1925 Size: 1679 6200 BssSI C'ACGA_G Cuts at: 5939 6951 5939 Size: 1012 6867 BstAPI GCAn_nnn'nTGC Cuts at: 794 3024 3586 794 Size: 2230 562 5087 Fragments arranged by size: 5087 2230 562 BstBI TT'CG_AA Cuts at: 4320 6683 4320 Size: 2363 5516 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7879 BstXI CCAn_nnnn'nTGG Cuts at: 2970 3077 2970 Size: 107 7772 BstYI r'GATC_y Cuts at: 1972 2895 4755 5209 5576 5961 5978 7430 7516 Size: 923 1860 454 367 385 17 1452 86 Cuts at: 7516 7528 7874 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1860 1452 923 454 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2326 3681 3822 3957 4076 4296 5143 Size: 552 1261 1355 141 135 119 220 847 Cuts at: 5143 513 Size: 3249 Fragments arranged by size: 3249 1355 1261 847 552 220 141 135 119 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7140 Fragments arranged by size: 7140 683 56 BtsI GCAGTG_nn' Cuts at: 2071 2199 2596 2782 4678 5006 5395 6189 6209 Size: 128 397 186 1896 328 389 794 20 Cuts at: 6209 2071 Size: 3741 Fragments arranged by size: 3741 1896 794 397 389 328 186 128 20 ClaI AT'CG_AT Cuts at: 1554 2940 1554 Size: 1386 6493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2818 2853 529 Size: 35 2254 35 5555 Fragments arranged by size: 5555 2254 35 35 DraI TTT'AAA Cuts at: 5183 5183 Size: 7879 DraIII CAC_nnn'GTG Cuts at: 2881 2881 Size: 7879 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6886 809 Size: 507 5570 1802 Fragments arranged by size: 5570 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2343 2442 2721 Size: 54 1425 50 275 18 513 99 279 Cuts at: 2721 3396 4716 4738 4873 5326 6215 7844 8 Size: 675 1320 22 135 453 889 1629 43 Fragments arranged by size: 1629 1425 1320 889 675 513 453 279 275 135 99 54 50 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 2442 5326 1537 Size: 275 630 2884 4090 Fragments arranged by size: 4090 2884 630 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2705 4026 4317 4389 5809 1353 Size: 1352 1321 291 72 1420 3423 Fragments arranged by size: 3423 1420 1352 1321 291 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2386 6466 6840 6986 387 Size: 1064 138 797 4080 374 146 1280 Fragments arranged by size: 4080 1280 1064 797 374 146 138 EcoICRI GAG'CTC Cuts at: 3249 3249 Size: 7879 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2522 2946 3159 3590 3620 3803 Size: 393 563 327 424 213 431 30 183 Cuts at: 3803 4166 4561 4682 4805 4814 4940 5942 6539 Size: 363 395 121 123 9 126 1002 597 Cuts at: 6539 7326 7594 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1002 787 597 563 431 424 395 393 363 327 268 213 183 126 123 121 30 9 EcoO109I rG'GnC_Cy Cuts at: 2311 3471 3472 3748 2311 Size: 1160 1 276 6442 Fragments arranged by size: 6442 1160 276 1 EcoRI G'AATT_C Cuts at: 5077 5188 5566 5077 Size: 111 378 7390 Fragments arranged by size: 7390 378 111 FspI TGC'GCA Cuts at: 2372 3632 3768 6385 2372 Size: 1260 136 2617 3866 Fragments arranged by size: 3866 2617 1260 136 FspAI rTGC'GCAy Cuts at: 2372 2372 Size: 7879 HaeII r_GCGC'y Cuts at: 1678 2146 2325 4459 7026 1678 Size: 468 179 2134 2567 2531 Fragments arranged by size: 2567 2531 2134 468 179 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2089 2121 2576 2608 2612 Size: 32 135 32 517 32 455 32 4 Cuts at: 2612 2644 3373 3405 4427 4459 6520 6552 6594 Size: 32 729 32 1022 32 2061 32 42 Cuts at: 6594 6626 1373 Size: 32 2626 Fragments arranged by size: 2626 2061 1022 729 517 455 135 42 32 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 5223 5469 669 Size: 988 281 3285 246 3079 Fragments arranged by size: 3285 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7879 HpaI GTT'AAC Cuts at: 5469 5469 Size: 7879 KpnI G_GTAC'C Cuts at: 3794 5204 3794 Size: 1410 6469 MfeI C'AATT_G Cuts at: 5478 5478 Size: 7879 MluI A'CGCG_T Cuts at: 7755 7755 Size: 7879 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4356 6993 7177 1305 Size: 3051 2637 184 2007 Fragments arranged by size: 3051 2637 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3398 4875 10 Size: 54 1425 343 1566 1477 3014 Fragments arranged by size: 3014 1566 1477 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2663 3092 5837 6196 6355 518 Size: 648 1497 429 2745 359 159 2042 Fragments arranged by size: 2745 2042 1497 648 429 359 159 MspA1I CmG'CkG Cuts at: 1606 2868 3406 3583 3703 3736 3959 4078 4156 Size: 1262 538 177 120 33 223 119 78 Cuts at: 4156 4206 4818 5295 5972 7120 7365 1606 Size: 50 612 477 677 1148 245 2120 Fragments arranged by size: 2120 1262 1148 677 612 538 477 245 223 177 120 119 78 50 33 NaeI GCC'GGC Cuts at: 2337 2337 Size: 7879 NcoI C'CATG_G Cuts at: 513 1065 3681 3822 513 Size: 552 2616 141 4570 Fragments arranged by size: 4570 2616 552 141 NdeI CA'TA_TG Cuts at: 387 387 Size: 7879 NgoMIV G'CCGG_C Cuts at: 2335 2335 Size: 7879 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7879 NotI GC'GGCC_GC Cuts at: 5326 5326 Size: 7879 NspI r_CATG'y Cuts at: 1132 1229 2560 2713 2985 4177 4419 4753 4939 Size: 97 1331 153 272 1192 242 334 186 Cuts at: 4939 5237 6782 1132 Size: 298 1545 2229 Fragments arranged by size: 2229 1545 1331 1192 334 298 272 242 186 153 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7879 PciI A'CATG_T Cuts at: 2709 6778 2709 Size: 4069 3810 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7879 PfoI T'CCnGG_A Cuts at: 2355 3461 4211 2355 Size: 1106 750 6023 Fragments arranged by size: 6023 1106 750 PmeI GTTT'AAAC Cuts at: 5183 5183 Size: 7879 PmlI CAC'GTG Cuts at: 2878 2878 Size: 7879 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3972 4004 4946 4978 5948 5980 7461 7493 3972 Size: 32 942 32 970 32 1481 32 4358 Fragments arranged by size: 4358 1481 970 942 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3748 3748 Size: 7879 PshAI GACnn'nnGTC Cuts at: 1613 4467 1613 Size: 2854 5025 PsiI TTA'TAA Cuts at: 5449 5449 Size: 7879 PspOMI G'GGCC_C Cuts at: 2799 3471 2799 Size: 672 7207 PspXI vC'TCGA_Gb Cuts at: 4600 4600 Size: 7879 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2411 2443 2411 Size: 32 7847 PstI C_TGCA'G Cuts at: 830 2041 2793 3495 3507 4392 5231 830 Size: 1211 752 702 12 885 839 3478 Fragments arranged by size: 3478 1211 885 839 752 702 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7879 PvuII CAG'CTG Cuts at: 3736 4156 3736 Size: 420 7459 SacI G_AGCT'C Cuts at: 3251 3251 Size: 7879 SacII CC_GC'GG Cuts at: 3960 4079 3960 Size: 119 7760 SalI G'TCGA_C Cuts at: 1936 5221 1936 Size: 3285 4594 SbfI CC_TGCA'GG Cuts at: 5231 5231 Size: 7879 ScaI AGT'ACT Cuts at: 3575 6127 7839 3575 Size: 2552 1712 3615 Fragments arranged by size: 3615 2552 1712 SfcI C'TryA_G Cuts at: 826 1045 2037 2789 3491 3503 3949 4388 5046 Size: 219 992 752 702 12 446 439 658 Cuts at: 5046 5227 6362 7043 7234 826 Size: 181 1135 681 191 1471 Fragments arranged by size: 1471 1135 992 752 702 681 658 446 439 219 191 181 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7879 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7879 SmaI CCC'GGG Cuts at: 1872 5206 1872 Size: 3334 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2106 2205 2263 3449 3647 3858 Size: 197 926 163 99 58 1186 198 211 Cuts at: 3858 4600 5983 6884 7146 820 Size: 742 1383 901 262 1553 Fragments arranged by size: 1553 1383 1186 926 901 742 262 211 198 197 163 99 58 SnaBI TAC'GTA Cuts at: 493 5670 493 Size: 5177 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7879 SphI G_CATG'C Cuts at: 1132 5237 1132 Size: 4105 3774 SspI AAT'ATT Cuts at: 5 52 3202 5803 5 Size: 47 3150 2601 2081 Fragments arranged by size: 3150 2601 2081 47 StuI AGG'CCT Cuts at: 1517 2410 3193 4037 1517 Size: 893 783 844 5359 Fragments arranged by size: 5359 893 844 783 StyI C'CwwG_G Cuts at: 513 1065 2364 3276 3681 3822 4394 4710 5029 Size: 552 1299 912 405 141 572 316 319 Cuts at: 5029 5318 513 Size: 289 3074 Fragments arranged by size: 3074 1299 912 572 552 405 319 316 289 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2999 5906 5923 6076 6261 6706 325 Size: 1255 1419 2907 17 153 185 445 1498 Fragments arranged by size: 2907 1498 1419 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2382 2418 3084 Size: 274 80 33 51 1443 405 36 666 Cuts at: 3084 3573 3924 6125 7837 96 Size: 489 351 2201 1712 138 Fragments arranged by size: 2201 1712 1443 666 489 405 351 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2636 2660 2668 2692 7839 7871 2636 Size: 24 8 24 5147 32 2644 Fragments arranged by size: 5147 2644 32 24 24 8 Tth111I GACn'n_nGTC Cuts at: 1316 2022 2534 4945 1316 Size: 706 512 2411 4250 Fragments arranged by size: 4250 2411 706 512 XbaI T'CTAG_A Cuts at: 5215 5215 Size: 7879 XhoI C'TCGA_G Cuts at: 1943 4600 1943 Size: 2657 5222 XmaI C'CCGG_G Cuts at: 1870 5204 1870 Size: 3334 4545 XmnI GAAnn'nnTTC Cuts at: 4365 4961 6008 4365 Size: 596 1047 6236 Fragments arranged by size: 6236 1047 596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5687 276 Size: 53 83 186 936 4153 2468 Fragments arranged by size: 4153 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI KasI NarI NruI NsiI PacI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI