(Circular) (Six-base) MAPSORT of: pFN21AB3758.seq Check: 6423 from: 1 to: 6487 pFN21AB3758 6487 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3358 3358 Size: 6487 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2526 4297 278 Size: 53 83 186 936 990 1771 2468 Fragments arranged by size: 2468 1771 990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3830 1937 Size: 1893 4594 Acc65I G'GTAC_C Cuts at: 3808 3808 Size: 6487 AclI AA'CG_TT Cuts at: 1563 4614 4987 1563 Size: 3051 373 3063 Fragments arranged by size: 3063 3051 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3200 4550 5934 6202 1632 Size: 1568 1350 1384 268 1917 Fragments arranged by size: 1917 1568 1384 1350 268 AfeI AGC'GCT Cuts at: 3194 3194 Size: 6487 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6290 AflIII A'CryG_T Cuts at: 1203 5386 6363 1203 Size: 4183 977 1327 Fragments arranged by size: 4183 1327 977 AgeI A'CCGG_T Cuts at: 5318 5318 Size: 6487 AhdI GACnn_n'nnGTC Cuts at: 5216 5216 Size: 6487 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6487 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6487 AlwNI CAG_nnn'CTG Cuts at: 2644 2780 3178 3278 3664 4230 5802 2644 Size: 136 398 100 386 566 1572 3329 Fragments arranged by size: 3329 1572 566 398 386 136 100 ApaI G_GGCC'C Cuts at: 3629 3745 3629 Size: 116 6371 ApaLI G'TGCA_C Cuts at: 2079 4544 5700 2079 Size: 2465 1156 2866 Fragments arranged by size: 2866 2465 1156 ApoI r'AATT_y Cuts at: 1493 3796 4022 4174 6283 1493 Size: 2303 226 152 2109 1697 Fragments arranged by size: 2303 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5041 160 Size: 872 4009 1606 Fragments arranged by size: 4009 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 AvaI C'yCGr_G Cuts at: 1870 1943 2011 2054 2160 2349 2423 3812 1870 Size: 73 68 43 106 189 74 1389 4545 Fragments arranged by size: 4545 1389 189 106 74 73 68 43 BamHI G'GATC_C Cuts at: 2156 3817 4184 2156 Size: 1661 367 4459 Fragments arranged by size: 4459 1661 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2247 3454 3487 3808 5263 Size: 325 217 627 460 1207 33 321 1455 Cuts at: 5263 618 Size: 1842 Fragments arranged by size: 1842 1455 1207 627 460 325 321 217 33 BanII G_rGCy'C Cuts at: 1391 2115 2209 2245 2705 2760 3246 3629 3745 Size: 724 94 36 460 55 486 383 116 Cuts at: 3745 3806 1391 Size: 61 4072 Fragments arranged by size: 4072 724 486 460 383 116 94 61 55 36 BbeI G_GCGC'C Cuts at: 2251 2251 Size: 6487 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2399 2526 2868 928 Size: 971 500 127 342 4547 Fragments arranged by size: 4547 971 500 342 127 BbvCI CC'TCA_GC Cuts at: 2092 2966 3237 3387 3495 3701 2092 Size: 874 271 150 108 206 4878 Fragments arranged by size: 4878 874 271 206 150 108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4678 4712 1903 Size: 34 50 34 2657 34 3678 Fragments arranged by size: 3678 2657 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4379 5589 4379 Size: 1210 5277 BclI T'GATC_A Cuts at: 1548 1548 Size: 6487 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2222 3611 5098 Size: 107 122 71 631 605 550 1389 1487 Cuts at: 5098 136 Size: 1525 Fragments arranged by size: 1525 1487 1389 631 605 550 122 107 71 BglII A'GATC_T Cuts at: 6482 6482 Size: 6487 BlpI GC'TnA_GC Cuts at: 2704 3096 3904 2704 Size: 392 808 5287 Fragments arranged by size: 5287 808 392 Bme1580I G_kGCm'C Cuts at: 1790 2083 2141 3459 3629 3734 3745 4548 5704 Size: 293 58 1318 170 105 11 803 1156 Cuts at: 5704 1790 Size: 2573 Fragments arranged by size: 2573 1318 1156 803 293 170 105 58 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6487 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2173 4218 5176 Size: 416 53 83 399 24 756 2045 958 Cuts at: 5176 442 Size: 1753 Fragments arranged by size: 2045 1753 958 756 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6487 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2247 2486 2567 2747 2996 3074 3584 3629 Size: 1008 239 81 180 249 78 510 45 Cuts at: 3629 3641 3701 5147 1239 Size: 12 60 1446 2579 Fragments arranged by size: 2579 1446 1008 510 249 239 180 81 78 60 45 12 Bpu10I CC'TnA_GC Cuts at: 2092 2439 2895 2966 3119 3237 3387 3495 3701 Size: 347 456 71 153 118 150 108 206 Cuts at: 3701 2092 Size: 4878 Fragments arranged by size: 4878 456 347 206 153 150 118 108 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3047 4612 5477 5775 3047 Size: 1565 865 298 3759 Fragments arranged by size: 3759 1565 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3530 5150 882 Size: 617 2031 1620 2219 Fragments arranged by size: 2219 2031 1620 617 BsaAI yAC'GTr Cuts at: 493 1204 4278 493 Size: 711 3074 2702 Fragments arranged by size: 3074 2702 711 BsaBI GATnn'nnATC Cuts at: 6481 6481 Size: 6487 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2248 2396 2523 4294 Size: 53 83 186 936 715 148 127 1771 Cuts at: 4294 4676 5311 275 Size: 382 635 1451 Fragments arranged by size: 1771 1451 936 715 635 382 186 148 127 83 53 BsaWI w'CCGG_w Cuts at: 2107 2201 2283 4920 5318 5592 5739 6150 2107 Size: 94 82 2637 398 274 147 411 2444 Fragments arranged by size: 2637 2444 411 398 274 147 94 82 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6262 Fragments arranged by size: 6262 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 2564 2687 2729 2750 2801 2822 2825 Size: 843 535 123 42 21 51 21 3 Cuts at: 2825 2993 2996 3254 3461 3479 3548 3566 1186 Size: 168 3 258 207 18 69 18 4107 Fragments arranged by size: 4107 843 535 258 207 168 123 69 51 42 21 21 18 18 3 3 BseYI C'CCAG_C Cuts at: 2062 2698 3469 3546 5690 6186 2062 Size: 636 771 77 2144 496 2363 Fragments arranged by size: 2363 2144 771 636 496 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2906 1163 Size: 573 1170 4744 Fragments arranged by size: 4744 1170 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3937 4698 5726 1540 Size: 275 186 1936 761 1028 2301 Fragments arranged by size: 2301 1936 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2083 2209 2760 2878 3120 3246 4548 4633 5704 Size: 126 551 118 242 126 1302 85 1071 Cuts at: 5704 2083 Size: 2866 Fragments arranged by size: 2866 1302 1071 551 242 126 126 118 85 BsmI GAATG_Cn' Cuts at: 2258 3998 4091 2258 Size: 1740 93 4654 Fragments arranged by size: 4654 1740 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3221 3258 3221 Size: 37 6450 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2115 2141 2209 2245 2705 2760 Size: 399 293 32 26 68 36 460 55 Cuts at: 2760 2878 3120 3246 3459 3629 3734 3745 3806 Size: 118 242 126 213 170 105 11 61 Cuts at: 3806 4548 4633 5704 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 460 399 293 242 213 170 126 118 105 85 68 61 55 36 32 26 11 BspEI T'CCGG_A Cuts at: 2107 6150 2107 Size: 4043 2444 BspHI T'CATG_A Cuts at: 4376 6106 4376 Size: 1730 4757 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2874 3358 3842 844 Size: 2030 484 484 3489 Fragments arranged by size: 3489 2030 484 484 BsrBI CCG'CTC Cuts at: 1394 1823 2105 4374 1394 Size: 429 282 2269 3507 Fragments arranged by size: 3507 2269 429 282 BsrDI GCAATG_nn' Cuts at: 65 1248 2915 4982 5156 65 Size: 1183 1667 2067 174 1396 Fragments arranged by size: 2067 1667 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2025 2043 2415 3490 5131 5318 1700 Size: 110 215 18 372 1075 1641 187 2869 Fragments arranged by size: 2869 1641 1075 372 215 187 110 18 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6487 BssHII G'CGCG_C Cuts at: 1925 2443 1925 Size: 518 5969 BssSI C'ACGA_G Cuts at: 4547 5559 4547 Size: 1012 5475 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6487 BstBI TT'CG_AA Cuts at: 5291 5291 Size: 6487 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6487 BstXI CCAn_nnnn'nTGG Cuts at: 2568 3622 2568 Size: 1054 5433 BstYI r'GATC_y Cuts at: 1972 2156 3817 4184 4569 4586 6038 6124 6136 Size: 184 1661 367 385 17 1452 86 12 Cuts at: 6136 6482 1972 Size: 346 1977 Fragments arranged by size: 1977 1661 1452 385 367 346 184 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3332 3332 Size: 6487 BtgI C'CryG_G Cuts at: 513 1065 2312 3736 513 Size: 552 1247 1424 3264 Fragments arranged by size: 3264 1424 1247 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5748 Fragments arranged by size: 5748 683 56 BtsI GCAGTG_nn' Cuts at: 2619 2876 2932 4003 4797 4817 2619 Size: 257 56 1071 794 20 4289 Fragments arranged by size: 4289 1071 794 257 56 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6487 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6452 DraI TTT'AAA Cuts at: 3791 3791 Size: 6487 DraIII CAC_nnn'GTG Cuts at: 2740 2740 Size: 6487 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5494 809 Size: 507 4178 1802 Fragments arranged by size: 4178 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2265 2359 2413 Size: 54 1425 50 275 18 435 94 54 Cuts at: 2413 2635 3666 3934 4823 6452 8 Size: 222 1031 268 889 1629 43 Fragments arranged by size: 1629 1425 1031 889 435 275 268 222 94 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3934 1537 Size: 275 2122 4090 Fragments arranged by size: 4090 2122 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2799 2826 2997 3216 3507 4417 1353 Size: 1446 27 171 219 291 910 3423 Fragments arranged by size: 3423 1446 910 291 219 171 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2484 5074 5448 5594 387 Size: 1064 138 895 2590 374 146 1280 Fragments arranged by size: 2590 1280 1064 895 374 146 138 EcoICRI GAG'CTC Cuts at: 2207 2758 3244 2207 Size: 551 486 5450 Fragments arranged by size: 5450 551 486 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2247 2486 2567 2747 2996 3074 3200 Size: 393 615 239 81 180 249 78 126 Cuts at: 3200 3584 3629 3641 3701 4550 5147 5934 6202 Size: 384 45 12 60 849 597 787 268 Cuts at: 6202 1239 Size: 1524 Fragments arranged by size: 1524 849 787 615 597 393 384 268 249 239 180 126 81 78 60 45 12 EcoO109I rG'GnC_Cy Cuts at: 2059 2611 3626 3741 3742 2059 Size: 552 1015 115 1 4804 Fragments arranged by size: 4804 1015 552 115 1 EcoRI G'AATT_C Cuts at: 3796 4174 3796 Size: 378 6109 EcoRV GAT'ATC Cuts at: 3415 3415 Size: 6487 FspI TGC'GCA Cuts at: 2121 4993 2121 Size: 2872 3615 HaeII r_GCGC'y Cuts at: 1678 2251 3196 5634 1678 Size: 573 945 2438 2531 Fragments arranged by size: 2531 2438 945 573 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2270 2302 3193 3225 3512 Size: 32 135 32 698 32 891 32 287 Cuts at: 3512 3544 5128 5160 5202 5234 1373 Size: 32 1584 32 42 32 2626 Fragments arranged by size: 2626 1584 891 698 287 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3831 4077 669 Size: 988 281 1893 246 3079 Fragments arranged by size: 3079 1893 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6487 HpaI GTT'AAC Cuts at: 4077 4077 Size: 6487 KasI G'GCGC_C Cuts at: 2247 2247 Size: 6487 KpnI G_GTAC'C Cuts at: 3812 3812 Size: 6487 MfeI C'AATT_G Cuts at: 4086 4086 Size: 6487 MluI A'CGCG_T Cuts at: 6363 6363 Size: 6487 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3084 5601 5785 1305 Size: 1779 2517 184 2007 Fragments arranged by size: 2517 2007 1779 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3668 10 Size: 54 1425 343 1836 2829 Fragments arranged by size: 2829 1836 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2883 4445 4804 4963 518 Size: 648 1717 1562 359 159 2042 Fragments arranged by size: 2042 1717 1562 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2381 2944 2995 3410 3618 3664 3700 3903 Size: 775 563 51 415 208 46 36 203 Cuts at: 3903 4580 5728 5973 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 775 677 563 415 245 208 203 51 46 36 NaeI GCC'GGC Cuts at: 2045 2417 3492 2045 Size: 372 1075 5040 Fragments arranged by size: 5040 1075 372 NarI GG'CG_CC Cuts at: 2248 2248 Size: 6487 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5935 NdeI CA'TA_TG Cuts at: 387 387 Size: 6487 NgoMIV G'CCGG_C Cuts at: 2043 2415 3490 2043 Size: 372 1075 5040 Fragments arranged by size: 5040 1075 372 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6487 NotI GC'GGCC_GC Cuts at: 3934 3934 Size: 6487 NspI r_CATG'y Cuts at: 1132 1229 3845 5390 1132 Size: 97 2616 1545 2229 Fragments arranged by size: 2616 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6487 PciI A'CATG_T Cuts at: 5386 5386 Size: 6487 PflMI CCAn_nnn'nTGG Cuts at: 1108 2644 3091 3664 1108 Size: 1536 447 573 3931 Fragments arranged by size: 3931 1536 573 447 PfoI T'CCnGG_A Cuts at: 3222 3222 Size: 6487 PmeI GTTT'AAAC Cuts at: 3791 3791 Size: 6487 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3030 3062 4556 4588 6069 6101 3030 Size: 32 1494 32 1481 32 3416 Fragments arranged by size: 3416 1494 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2611 2059 Size: 552 5935 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6487 PsiI TTA'TAA Cuts at: 4057 4057 Size: 6487 PspOMI G'GGCC_C Cuts at: 3625 3741 3625 Size: 116 6371 PstI C_TGCA'G Cuts at: 830 2601 2626 2871 3168 3189 3773 3839 830 Size: 1771 25 245 297 21 584 66 3478 Fragments arranged by size: 3478 1771 584 297 245 66 25 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6487 PvuII CAG'CTG Cuts at: 2944 2995 3664 3700 2944 Size: 51 669 36 5731 Fragments arranged by size: 5731 669 51 36 SacI G_AGCT'C Cuts at: 2209 2760 3246 2209 Size: 551 486 5450 Fragments arranged by size: 5450 551 486 SalI G'TCGA_C Cuts at: 1936 3829 1936 Size: 1893 4594 SanDI GG'GwC_CC Cuts at: 2059 2059 Size: 6487 SbfI CC_TGCA'GG Cuts at: 3839 3839 Size: 6487 ScaI AGT'ACT Cuts at: 4735 6447 4735 Size: 1712 4775 SexAI A'CCwGG_T Cuts at: 2736 2736 Size: 6487 SfcI C'TryA_G Cuts at: 826 1045 2597 2622 2867 3164 3185 3349 3769 Size: 219 1552 25 245 297 21 164 420 Cuts at: 3769 3835 4970 5651 5842 826 Size: 66 1135 681 191 1471 Fragments arranged by size: 1552 1471 1135 681 420 297 245 219 191 164 66 25 21 SfoI GGC'GCC Cuts at: 2249 2249 Size: 6487 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6487 SmaI CCC'GGG Cuts at: 1872 2056 2351 2425 3814 1872 Size: 184 295 74 1389 4545 Fragments arranged by size: 4545 1389 295 184 74 SmlI C'TyrA_G Cuts at: 820 1017 1943 3026 4591 5492 5754 820 Size: 197 926 1083 1565 901 262 1553 Fragments arranged by size: 1565 1553 1083 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4278 493 Size: 3785 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6487 SphI G_CATG'C Cuts at: 1132 3845 1132 Size: 2713 3774 SspI AAT'ATT Cuts at: 5 52 4411 5 Size: 47 4359 2081 Fragments arranged by size: 4359 2081 47 StuI AGG'CCT Cuts at: 1517 3008 1517 Size: 1491 4996 StyI C'CwwG_G Cuts at: 513 1065 2232 2272 2332 2709 3926 513 Size: 552 1167 40 60 377 1217 3074 Fragments arranged by size: 3074 1217 1167 552 377 60 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2528 3554 4514 4531 4684 4869 5314 Size: 1255 948 1026 960 17 153 185 445 Cuts at: 5314 325 Size: 1498 Fragments arranged by size: 1498 1255 1026 960 948 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4733 6445 96 Size: 274 80 33 51 1443 2756 1712 138 Fragments arranged by size: 2756 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2297 2329 2462 2494 6447 6479 2297 Size: 32 133 32 3953 32 2305 Fragments arranged by size: 3953 2305 133 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6487 XbaI T'CTAG_A Cuts at: 3823 3823 Size: 6487 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2339 3088 2339 Size: 749 5738 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6487 XmaI C'CCGG_G Cuts at: 1870 2054 2349 2423 3812 1870 Size: 184 295 74 1389 4545 Fragments arranged by size: 4545 1389 295 184 74 XmnI GAAnn'nnTTC Cuts at: 4616 4616 Size: 6487 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2524 4295 276 Size: 53 83 186 936 990 1771 2468 Fragments arranged by size: 2468 1771 990 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoNI FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI