Restriction map for pFN21AB3758
 (Linear) (Six-base) MAPSORT of: KIBB3758.seq  Check: 7614  from: 1  to: 1782



KIBB3758.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1353    1782 
   Size:       1353     429
 
AatII G_ACGT'C

Cuts at:      0     521    1782 
   Size:        521    1261
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1195    1782 
   Size:       1195     587
 
AfeI AGC'GCT

Cuts at:      0    1189    1782 
   Size:       1189     593
 
AlwNI CAG_nnn'CTG

Cuts at:      0     639     775    1173    1273    1659    1782 
   Size:        639     136     398     100     386     123

  Fragments arranged by size: 

                 639     398     386     136     123     100
 
ApaI G_GGCC'C

Cuts at:      0    1624    1740    1782 
   Size:       1624     116      42

  Fragments arranged by size: 

                1624     116      42
 
ApaLI G'TGCA_C

Cuts at:      0      74    1782 
   Size:         74    1708
 
AvaI C'yCGr_G

Cuts at:      0       6      49     155     344     418    1782 
   Size:          6      43     106     189      74    1364

  Fragments arranged by size: 

                1364     189     106      74      43       6
 
BamHI G'GATC_C

Cuts at:      0     151    1782 
   Size:        151    1631
 
BanI G'GyrC_C

Cuts at:      0     242    1449    1482    1782 
   Size:        242    1207      33     300

  Fragments arranged by size: 

                1207     300     242      33
 
BanII G_rGCy'C

Cuts at:      0     110     204     240     700     755    1241    1624    1740 
   Size:        110      94      36     460      55     486     383     116

Cuts at:   1740    1782 
   Size:         42

  Fragments arranged by size: 

                 486     460     383     116     110      94      55      42
                  36
 
BbeI G_GCGC'C

Cuts at:      0     246    1782 
   Size:        246    1536
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     394     521     863    1782 
   Size:        394     127     342     919

  Fragments arranged by size: 

                 919     394     342     127
 
BbvCI CC'TCA_GC

Cuts at:      0      87     961    1232    1382    1490    1696    1782 
   Size:         87     874     271     150     108     206      86

  Fragments arranged by size: 

                 874     271     206     150     108      87      86
 
BglI GCCn_nnn'nGGC

Cuts at:      0     217    1606    1782 
   Size:        217    1389     176

  Fragments arranged by size: 

                1389     217     176
 
BlpI GC'TnA_GC

Cuts at:      0     699    1091    1782 
   Size:        699     392     691

  Fragments arranged by size: 

                 699     691     392
 
Bme1580I G_kGCm'C

Cuts at:      0      78     136    1454    1624    1729    1740    1782 
   Size:         78      58    1318     170     105      11      42

  Fragments arranged by size: 

                1318     170     105      78      58      42      11
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     168    1782 
   Size:        168    1614
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     481     562     742     991    1069    1579    1624 
   Size:        242     239      81     180     249      78     510      45

Cuts at:   1624    1636    1696    1782 
   Size:         12      60      86

  Fragments arranged by size: 

                 510     249     242     239     180      86      81      78
                  60      45      12
 
Bpu10I CC'TnA_GC

Cuts at:      0      87     434     890     961    1114    1232    1382    1490 
   Size:         87     347     456      71     153     118     150     108

Cuts at:   1490    1696    1782 
   Size:        206      86

  Fragments arranged by size: 

                 456     347     206     153     150     118     108      87
                  86      71
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1042    1782 
   Size:       1042     740
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1525    1782 
   Size:       1525     257
 
BsaHI Gr'CG_yC

Cuts at:      0     243     391     518    1782 
   Size:        243     148     127    1264

  Fragments arranged by size: 

                1264     243     148     127
 
BsaWI w'CCGG_w

Cuts at:      0     102     196     278    1782 
   Size:        102      94      82    1504

  Fragments arranged by size: 

                1504     102      94      82
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      24     559     682     724     745     796     817     820 
   Size:         24     535     123      42      21      51      21       3

Cuts at:    820     988     991    1249    1456    1474    1543    1561    1782 
   Size:        168       3     258     207      18      69      18     221

  Fragments arranged by size: 

                 535     258     221     207     168     123      69      51
                  42      24      21      21      18      18       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0      57     693    1464    1541    1782 
   Size:         57     636     771      77     241

  Fragments arranged by size: 

                 771     636     241      77      57
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     901    1782 
   Size:        901     881
 
BsiHKAI G_wGCw'C

Cuts at:      0      78     204     755     873    1115    1241    1782 
   Size:         78     126     551     118     242     126     541

  Fragments arranged by size: 

                 551     541     242     126     126     118      78
 
BsmI GAATG_Cn'

Cuts at:      0     253    1782 
   Size:        253    1529
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1216    1253    1782 
   Size:       1216      37     529

  Fragments arranged by size: 

                1216     529      37
 
Bsp1286I G_dGCh'C

Cuts at:      0      78     110     136     204     240     700     755     873 
   Size:         78      32      26      68      36     460      55     118

Cuts at:    873    1115    1241    1454    1624    1729    1740    1782 
   Size:        242     126     213     170     105      11      42

  Fragments arranged by size: 

                 460     242     213     170     126     118     105      78
                  68      55      42      36      32      26      11
 
BspEI T'CCGG_A

Cuts at:      0     102    1782 
   Size:        102    1680
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     869    1353    1782 
   Size:        869     484     429

  Fragments arranged by size: 

                 869     484     429
 
BsrBI CCG'CTC

Cuts at:      0     100    1782 
   Size:        100    1682
 
BsrDI GCAATG_nn'

Cuts at:      0     910    1782 
   Size:        910     872
 
BsrFI r'CCGG_y

Cuts at:      0      20      38     410    1485    1782 
   Size:         20      18     372    1075     297

  Fragments arranged by size: 

                1075     372     297      20      18
 
BssHII G'CGCG_C

Cuts at:      0     438    1782 
   Size:        438    1344
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     563    1617    1782 
   Size:        563    1054     165

  Fragments arranged by size: 

                1054     563     165
 
BstYI r'GATC_y

Cuts at:      0     151    1782 
   Size:        151    1631
 
Bsu36I CC'TnA_GG

Cuts at:      0    1327    1782 
   Size:       1327     455
 
BtgI C'CryG_G

Cuts at:      0     307    1731    1782 
   Size:        307    1424      51

  Fragments arranged by size: 

                1424     307      51
 
BtsI GCAGTG_nn'

Cuts at:      0     614     871     927    1782 
   Size:        614     257      56     855

  Fragments arranged by size: 

                 855     614     257      56
 
DraIII CAC_nnn'GTG

Cuts at:      0     735    1782 
   Size:        735    1047
 
EaeI y'GGCC_r

Cuts at:      0     260     354     408     630    1661    1782 
   Size:        260      94      54     222    1031     121

  Fragments arranged by size: 

                1031     260     222     121      94      54
 
EarI CTCTTCn'nnn_

Cuts at:      0     794     821     992    1211    1502    1782 
   Size:        794      27     171     219     291     280

  Fragments arranged by size: 

                 794     291     280     219     171      27
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     479    1782 
   Size:        479    1303
 
EcoICRI GAG'CTC

Cuts at:      0     202     753    1239    1782 
   Size:        202     551     486     543

  Fragments arranged by size: 

                 551     543     486     202
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     481     562     742     991    1069    1195    1579 
   Size:        242     239      81     180     249      78     126     384

Cuts at:   1579    1624    1636    1696    1782 
   Size:         45      12      60      86

  Fragments arranged by size: 

                 384     249     242     239     180     126      86      81
                  78      60      45      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54     606    1621    1736    1737    1782 
   Size:         54     552    1015     115       1      45

  Fragments arranged by size: 

                1015     552     115      54      45       1
 
EcoRV GAT'ATC

Cuts at:      0    1410    1782 
   Size:       1410     372
 
FspI TGC'GCA

Cuts at:      0     116    1782 
   Size:        116    1666
 
HaeII r_GCGC'y

Cuts at:      0     246    1191    1782 
   Size:        246     945     591

  Fragments arranged by size: 

                 945     591     246
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     265     297    1188    1220    1507    1539    1782 
   Size:        265      32     891      32     287      32     243

  Fragments arranged by size: 

                 891     287     265     243      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     242    1782 
   Size:        242    1540
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1079    1782 
   Size:       1079     703
 
MscI TGG'CCA

Cuts at:      0    1663    1782 
   Size:       1663     119
 
MslI CAynn'nnrTG

Cuts at:      0     878    1782 
   Size:        878     904
 
MspA1I CmG'CkG

Cuts at:      0     376     939     990    1405    1613    1659    1695    1782 
   Size:        376     563      51     415     208      46      36      87

  Fragments arranged by size: 

                 563     415     376     208      87      51      46      36
            
 
NaeI GCC'GGC

Cuts at:      0      40     412    1487    1782 
   Size:         40     372    1075     295

  Fragments arranged by size: 

                1075     372     295      40
 
NarI GG'CG_CC

Cuts at:      0     243    1782 
   Size:        243    1539
 
NgoMIV G'CCGG_C

Cuts at:      0      38     410    1485    1782 
   Size:         38     372    1075     297

  Fragments arranged by size: 

                1075     372     297      38
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     639    1086    1659    1782 
   Size:        639     447     573     123

  Fragments arranged by size: 

                 639     573     447     123
 
PfoI T'CCnGG_A

Cuts at:      0    1217    1782 
   Size:       1217     565
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1025    1057    1782 
   Size:       1025      32     725

  Fragments arranged by size: 

                1025     725      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      54     606    1782 
   Size:         54     552    1176

  Fragments arranged by size: 

                1176     552      54
 
PspOMI G'GGCC_C

Cuts at:      0    1620    1736    1782 
   Size:       1620     116      46

  Fragments arranged by size: 

                1620     116      46
 
PstI C_TGCA'G

Cuts at:      0     596     621     866    1163    1184    1768    1782 
   Size:        596      25     245     297      21     584      14

  Fragments arranged by size: 

                 596     584     297     245      25      21      14
 
PvuII CAG'CTG

Cuts at:      0     939     990    1659    1695    1782 
   Size:        939      51     669      36      87

  Fragments arranged by size: 

                 939     669      87      51      36
 
SacI G_AGCT'C

Cuts at:      0     204     755    1241    1782 
   Size:        204     551     486     541

  Fragments arranged by size: 

                 551     541     486     204
 
SanDI GG'GwC_CC

Cuts at:      0      54    1782 
   Size:         54    1728
 
SexAI A'CCwGG_T

Cuts at:      0     731    1782 
   Size:        731    1051
 
SfcI C'TryA_G

Cuts at:      0     592     617     862    1159    1180    1344    1764    1782 
   Size:        592      25     245     297      21     164     420      18

  Fragments arranged by size: 

                 592     420     297     245     164      25      21      18
            
 
SfoI GGC'GCC

Cuts at:      0     244    1782 
   Size:        244    1538
 
SmaI CCC'GGG

Cuts at:      0      51     346     420    1782 
   Size:         51     295      74    1362

  Fragments arranged by size: 

                1362     295      74      51
 
SmlI C'TyrA_G

Cuts at:      0    1021    1782 
   Size:       1021     761
 
StuI AGG'CCT

Cuts at:      0    1003    1782 
   Size:       1003     779
 
StyI C'CwwG_G

Cuts at:      0     227     267     327     704    1782 
   Size:        227      40      60     377    1078

  Fragments arranged by size: 

                1078     377     227      60      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     523    1549    1782 
   Size:        523    1026     233

  Fragments arranged by size: 

                1026     523     233
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     292     324     457     489    1782 
   Size:        292      32     133      32    1293

  Fragments arranged by size: 

                1293     292     133      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     334    1083    1782 
   Size:        334     749     699

  Fragments arranged by size: 

                 749     699     334
 
XmaI C'CCGG_G

Cuts at:      0      49     344     418    1782 
   Size:         49     295      74    1364

  Fragments arranged by size: 

                1364     295      74      49
 
ZraI GAC'GTC

Cuts at:      0     519    1782 
   Size:        519    1263
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI    AlwNI     ApaI    ApaLI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BglI     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BstXI    BstYI   Bsu36I
     BtgI     BtsI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRV     FspI    HaeII    Hin4I     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV    PflMI     PfoI     PpiI
    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI    SexAI     SfcI
     SfoI     SmaI     SmlI     StuI     StyI    TaqII     TstI     XcmI
     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI     BplI
    BsaAI    BsaBI    BsaXI    BsiEI    BsiWI    BspHI    BsrGI    BssSI
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI    EcoNI    EcoRI     FalI     FseI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI  Tth111I     XbaI
     XhoI     XmnI
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