Restriction map for
pFN21AB3552
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BalI BamHI BbrPI BbsI BciVI
BclI BfrI BglII BpuAI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Cfr10I
ClaI CpoI DpnII DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeII MamI MboI MboII MfeI
MflI MluI MluNI MroI MscI
MseI MunI MvnI NaeI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacII SalI SapI Sau3AI ScaI
SexAI SfuI SgrAI SmlI SnaBI
SpeI SspBI SspI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcyI 202 GGCGCC G[GA]^CG[CT]C
AocI 249 CCTGAGG CC^T[ACGT]AGG
AvaII 729 GGACC G^G[AT]CC
AvrII 1021 CCTAGG C^CTAGG
BbeI 202 GGCGCC GGCGC^C
BbvCI 496 GCTGAGG CCTCAGC(-5/-2)
BcgI 150 GCAGCGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1021 CCTAGG C^CTAGG
BsaHI 202 GGCGCC G[GA]^CG[CT]C
BsaI 69 GGTCTC GGTCTC(1/5)
BsmAI 70 GTCTC GTCTC(1/5)
BsmI 492 GAATGC GAATGC(1/-1)
Bsu36I 249 CCTGAGG CC^T[ACGT]AGG
DraIII 584 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 249 CCTGAGG CC^T[ACGT]AGG
EcoNI 720 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 202 GGCGCC G[GA]^CG[CT]C
KasI 202 GGCGCC G^GCGCC
MaeIII 433 GTGAC ^GT[ACGT]AC
NarI 202 GGCGCC GG^CGCC
NcoI 20 CCATGG C^CATGG
NspI 606 GCATGC [GA]CATG^[CT]
PpuMI 728 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 292 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 576 CCTGCAGG CCTGCA^GG
SfiI 124 GGCCAGACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 202 GGCGCC GGC^GCC
SmaI 551 CCCGGG CCC^GGG
SphI 606 GCATGC GCATG^C
Sse8387I 576 CCTGCAGG CCTGCA^GG
StuI 515 AGGCCT AGG^CCT
Tsp45I 433 GTGAC ^GT[GC]AC
Tsp509I 321 AATT ^AATT
XmaCI 551 CCCGGG C^CCGGG
XmaI 551 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AspHI 475 GAGCTC G[AT]GC[AT]^C
856 GAGCTC
BanI 202 GGCGCC G^G[CT][GA]CC
1012 GGTGCC
BlpI 601 GCTGAGC GC^T[ACGT]AGC
655 GCTGAGC
Bpu1102I 601 GCTGAGC GC^T[ACGT]AGC
655 GCTGAGC
BsiHKAI 475 GAGCTC G[AT]GC[AT]^C
856 GAGCTC
BsrBI 169 GAGCGG CCGCTC(-3/-3)
745 GAGCGG
CelII 601 GCTGAGC GC^T[ACGT]AGC
655 GCTGAGC
EcoT14I 20 CCATGG C^C[AT][AT]GG
1021 CCTAGG
FauI 371 CCCGC CCCGC(4/6)
465 GCGGG
HgaI 631 GACGC GACGC(5/10)
1045 GACGC
HinfI 976 GAGTC G^A[ACGT]TC
1000 GAGTC
HphI 783 GGTGA GGTGA(8/7)
694 TCACC
MslI 185 CACAAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1006 CATGCTGGTG
PleI 976 GAGTC GAGTC(4/5)
1000 GAGTC
PvuII 294 CAGCTG CAG^CTG
664 CAGCTG
SacI 475 GAGCTC GAGCT^C
856 GAGCTC
SfaNI 47 GCATC GCATC(5/9)
703 GCATC
StyI 20 CCATGG C^C[AT][AT]GG
1021 CCTAGG
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwNI ApaI AvaI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MaeI
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PflMI PstI
Sau96I ScrFI SfcI TseI TspRI
Van91I