(Circular) (Six-base) MAPSORT of: pFN21AB3552.seq Check: 9639 from: 1 to: 5824 pFN21AB3552 5824 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2099 2228 2099 Size: 129 5695 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3167 1937 Size: 1230 4594 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 3887 5271 5539 1632 Size: 422 1833 1384 268 1917 Fragments arranged by size: 1917 1833 1384 422 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 4723 5700 1203 Size: 3520 977 1327 Fragments arranged by size: 3520 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 2194 1166 Size: 1028 4796 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2483 2514 1184 Size: 1299 31 4494 Fragments arranged by size: 4494 1299 31 AlwNI CAG_nnn'CTG Cuts at: 2185 2632 2737 2842 3080 3121 3567 5139 2185 Size: 447 105 105 238 41 446 1572 2870 Fragments arranged by size: 2870 1572 447 446 238 105 105 41 ApaI G_GGCC'C Cuts at: 2271 2694 2800 2942 2960 2271 Size: 423 106 142 18 5135 Fragments arranged by size: 5135 423 142 106 18 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 3133 3359 3511 5620 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2393 2556 3033 3149 1870 Size: 73 450 163 477 116 4545 Fragments arranged by size: 4545 477 450 163 116 73 AvrII C'CTAG_G Cuts at: 3026 3026 Size: 5824 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2207 3017 3145 4600 618 Size: 325 217 627 420 810 128 1455 1842 Fragments arranged by size: 1842 1455 810 627 420 325 217 128 BanII G_rGCy'C Cuts at: 1391 2271 2376 2484 2694 2800 2865 2942 2960 Size: 880 105 108 210 106 65 77 18 Cuts at: 2960 3143 1391 Size: 183 4072 Fragments arranged by size: 4072 880 210 183 108 106 105 77 65 18 BbeI G_GCGC'C Cuts at: 2211 2211 Size: 5824 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4853 BbvCI CC'TCA_GC Cuts at: 2502 2502 Size: 5824 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2144 2178 4015 4049 1903 Size: 34 207 34 1837 34 3678 Fragments arranged by size: 3678 1837 207 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2136 2657 2804 Size: 107 122 71 631 605 464 521 147 Cuts at: 2804 2825 2926 2946 4435 136 Size: 21 101 20 1489 1525 Fragments arranged by size: 1525 1489 631 605 521 464 147 122 107 101 71 21 20 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 2607 2661 3241 2607 Size: 54 580 5190 Fragments arranged by size: 5190 580 54 Bme1580I G_kGCm'C Cuts at: 1790 2271 2653 2694 2800 2942 2960 3022 3885 Size: 481 382 41 106 142 18 62 863 Cuts at: 3885 5041 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 863 481 382 142 106 62 41 18 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3555 4513 442 Size: 416 53 83 399 24 2138 958 1753 Fragments arranged by size: 2138 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2498 2675 2822 2864 2952 3083 4484 1239 Size: 1259 177 147 42 88 131 1401 2579 Fragments arranged by size: 2579 1401 1259 177 147 131 88 42 Bpu10I CC'TnA_GC Cuts at: 2483 2502 2640 2829 2835 2483 Size: 19 138 189 6 5472 Fragments arranged by size: 5472 189 138 19 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3949 4814 5112 3949 Size: 865 298 4661 Fragments arranged by size: 4661 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2080 4487 882 Size: 617 581 2407 2219 Fragments arranged by size: 2407 2219 617 581 BsaAI yAC'GTr Cuts at: 493 1204 3615 493 Size: 711 2411 2702 Fragments arranged by size: 2702 2411 711 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2208 3631 4013 4648 Size: 53 83 186 936 675 1423 382 635 Cuts at: 4648 275 Size: 1451 Fragments arranged by size: 1451 1423 936 675 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4257 4655 4929 5076 5487 4257 Size: 398 274 147 411 4594 Fragments arranged by size: 4594 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2165 2195 1178 Size: 30 165 30 762 30 4807 Fragments arranged by size: 4807 762 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2281 2519 2861 2993 1186 Size: 1095 238 342 132 4017 Fragments arranged by size: 4017 1095 342 238 132 BseYI C'CCAG_C Cuts at: 2270 2279 2362 2405 2509 3021 3108 5027 5523 Size: 9 83 43 104 512 87 1919 496 Cuts at: 5523 2270 Size: 2571 Fragments arranged by size: 2571 1919 512 496 104 87 83 43 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2174 2443 2510 2711 2789 1163 Size: 573 438 269 67 201 78 4198 Fragments arranged by size: 4198 573 438 269 201 78 67 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2484 2865 3885 3970 5041 2484 Size: 381 1020 85 1071 3267 Fragments arranged by size: 3267 1071 1020 381 85 BsmI GAATG_Cn' Cuts at: 2503 3335 3428 2503 Size: 832 93 4899 Fragments arranged by size: 4899 832 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2271 2376 2484 2653 2694 2800 2865 Size: 399 481 105 108 169 41 106 65 Cuts at: 2865 2942 2960 3022 3143 3885 3970 5041 1391 Size: 77 18 62 121 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 481 399 169 121 108 106 105 85 77 65 62 41 18 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2099 2228 2475 2886 3179 844 Size: 1255 129 247 411 293 3489 Fragments arranged by size: 3489 1255 411 293 247 129 BsrBI CCG'CTC Cuts at: 1394 1823 2176 2752 3711 1394 Size: 429 353 576 959 3507 Fragments arranged by size: 3507 959 576 429 353 BsrDI GCAATG_nn' Cuts at: 65 1248 4319 4493 65 Size: 1183 3071 174 1396 Fragments arranged by size: 3071 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4468 4655 1700 Size: 110 2658 187 2869 Fragments arranged by size: 2869 2658 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 2185 2539 2594 3080 794 Size: 1391 354 55 486 3538 Fragments arranged by size: 3538 1391 486 354 55 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5824 BstYI r'GATC_y Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819 Size: 1182 367 385 17 1452 86 12 346 Cuts at: 5819 1972 Size: 1977 Fragments arranged by size: 1977 1452 1182 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2255 2255 Size: 5824 BtgI C'CryG_G Cuts at: 513 1065 2025 2351 2924 513 Size: 552 960 326 573 3413 Fragments arranged by size: 3413 960 573 552 326 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2092 2253 487 Size: 683 56 866 161 4058 Fragments arranged by size: 4058 866 683 161 56 BtsI GCAGTG_nn' Cuts at: 2601 3340 4134 4154 2601 Size: 739 794 20 4271 Fragments arranged by size: 4271 794 739 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DraIII CAC_nnn'GTG Cuts at: 2594 2594 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2137 2598 Size: 54 1425 50 275 18 192 115 461 Cuts at: 2598 3271 4160 5789 8 Size: 673 889 1629 43 Fragments arranged by size: 1629 1425 889 673 461 275 192 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3754 1353 Size: 2401 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2482 2863 2482 Size: 381 5443 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2498 2675 2822 2864 2952 3083 Size: 393 422 444 177 147 42 88 131 Cuts at: 3083 3887 4484 5271 5539 1239 Size: 804 597 787 268 1524 Fragments arranged by size: 1524 804 787 597 444 422 393 268 177 147 131 88 42 EcoNI CCTnn'n_nnAGG Cuts at: 2729 2729 Size: 5824 EcoO109I rG'GnC_Cy Cuts at: 2267 2286 2473 2629 2683 2691 2734 2797 2938 Size: 19 187 156 54 8 43 63 141 Cuts at: 2938 2939 2956 2957 3030 2267 Size: 1 17 1 73 5061 Fragments arranged by size: 5061 187 156 141 73 63 54 43 19 17 8 1 1 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2211 2725 2997 4971 1678 Size: 533 514 272 1974 2531 Fragments arranged by size: 2531 1974 533 514 272 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4465 4497 4539 4571 1373 Size: 32 135 32 2893 32 42 32 2626 Fragments arranged by size: 2893 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3168 3414 669 Size: 988 281 1230 246 3079 Fragments arranged by size: 3079 1230 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KasI G'GCGC_C Cuts at: 2207 2207 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 5122 1305 Size: 3633 184 2007 Fragments arranged by size: 3633 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4002 Fragments arranged by size: 4002 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2194 3015 3782 4141 4300 518 Size: 648 1028 821 767 359 159 2042 Fragments arranged by size: 2042 1028 821 767 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2158 2301 2671 2678 3240 3917 5065 5310 Size: 552 143 370 7 562 677 1148 245 Cuts at: 5310 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 562 552 370 245 143 7 NarI GG'CG_CC Cuts at: 2208 2208 Size: 5824 NcoI C'CATG_G Cuts at: 513 1065 2025 513 Size: 552 960 4312 Fragments arranged by size: 4312 960 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 2615 3182 4727 1132 Size: 97 1386 567 1545 2229 Fragments arranged by size: 2229 1545 1386 567 97 PasI CC'CwG_GG Cuts at: 1348 2420 2687 1348 Size: 1072 267 4485 Fragments arranged by size: 4485 1072 267 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 2062 2101 2737 3121 1108 Size: 954 39 636 384 3811 Fragments arranged by size: 3811 954 636 384 39 PfoI T'CCnGG_A Cuts at: 3060 3060 Size: 5824 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2734 2734 Size: 5824 PshAI GACnn'nnGTC Cuts at: 1613 2301 1613 Size: 688 5136 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2267 2690 2796 2938 2956 2267 Size: 423 106 142 18 5135 Fragments arranged by size: 5135 423 142 106 18 PstI C_TGCA'G Cuts at: 830 2586 2628 2670 2883 3176 830 Size: 1756 42 42 213 293 3478 Fragments arranged by size: 3478 1756 293 213 42 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 PvuII CAG'CTG Cuts at: 2301 2671 2301 Size: 370 5454 SacI G_AGCT'C Cuts at: 2484 2865 2484 Size: 381 5443 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SbfI CC_TGCA'GG Cuts at: 2586 3176 2586 Size: 590 5234 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SfcI C'TryA_G Cuts at: 826 1045 2582 2624 2666 2879 3172 4307 4988 Size: 219 1537 42 42 213 293 1135 681 Cuts at: 4988 5179 826 Size: 191 1471 Fragments arranged by size: 1537 1471 1135 681 293 219 213 191 42 42 SfiI GGCCn_nnn'nGGCC Cuts at: 2136 2136 Size: 5824 SfoI GGC'GCC Cuts at: 2209 2209 Size: 5824 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 2558 3151 1872 Size: 686 593 4545 Fragments arranged by size: 4545 686 593 SmlI C'TyrA_G Cuts at: 820 1017 1943 3928 4829 5091 820 Size: 197 926 1985 901 262 1553 Fragments arranged by size: 1985 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 2615 3182 1132 Size: 1483 567 3774 Fragments arranged by size: 3774 1483 567 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 2522 1517 Size: 1005 4819 StyI C'CwwG_G Cuts at: 513 1065 2025 3026 3263 513 Size: 552 960 1001 237 3074 Fragments arranged by size: 3074 1001 960 552 237 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2032 2458 3851 3868 4021 4206 4651 Size: 1255 452 426 1393 17 153 185 445 Cuts at: 4651 325 Size: 1498 Fragments arranged by size: 1498 1393 1255 452 445 426 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4070 5782 96 Size: 274 80 33 51 1443 2093 1712 138 Fragments arranged by size: 2093 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5784 5816 5784 Size: 32 5792 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 2556 3149 1870 Size: 686 593 4545 Fragments arranged by size: 4545 686 593 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI XcmI