Restriction map for
pFN21AB3552
(Linear) (Six-base) MAPSORT of: KIBB3552.seq Check: 5007 from: 1 to: 1119
KIBB3552.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 94 223 1119
Size: 94 129 896
Fragments arranged by size:
896 129 94
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 1119
Size: 49 1070
AleI CACnn'nnGTG
Cuts at: 0 189 1119
Size: 189 930
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 478 509 1119
Size: 478 31 610
Fragments arranged by size:
610 478 31
AlwNI CAG_nnn'CTG
Cuts at: 0 180 627 732 837 1075 1116 1119
Size: 180 447 105 105 238 41 3
Fragments arranged by size:
447 238 180 105 105 41 3
ApaI G_GGCC'C
Cuts at: 0 266 689 795 937 955 1119
Size: 266 423 106 142 18 164
Fragments arranged by size:
423 266 164 142 106 18
AvaI C'yCGr_G
Cuts at: 0 388 551 1028 1119
Size: 388 163 477 91
Fragments arranged by size:
477 388 163 91
AvrII C'CTAG_G
Cuts at: 0 1021 1119
Size: 1021 98
BanI G'GyrC_C
Cuts at: 0 202 1012 1119
Size: 202 810 107
Fragments arranged by size:
810 202 107
BanII G_rGCy'C
Cuts at: 0 266 371 479 689 795 860 937 955
Size: 266 105 108 210 106 65 77 18
Cuts at: 955 1119
Size: 164
Fragments arranged by size:
266 210 164 108 106 105 77 65
18
BbeI G_GCGC'C
Cuts at: 0 206 1119
Size: 206 913
BbvCI CC'TCA_GC
Cuts at: 0 497 1119
Size: 497 622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 139 173 1119
Size: 139 34 946
Fragments arranged by size:
946 139 34
BglI GCCn_nnn'nGGC
Cuts at: 0 131 652 799 820 921 941 1119
Size: 131 521 147 21 101 20 178
Fragments arranged by size:
521 178 147 131 101 21 20
BlpI GC'TnA_GC
Cuts at: 0 602 656 1119
Size: 602 54 463
Fragments arranged by size:
602 463 54
Bme1580I G_kGCm'C
Cuts at: 0 266 648 689 795 937 955 1017 1119
Size: 266 382 41 106 142 18 62 102
Fragments arranged by size:
382 266 142 106 102 62 41 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 670 817 859 947 1078 1119
Size: 493 177 147 42 88 131 41
Fragments arranged by size:
493 177 147 131 88 42 41
Bpu10I CC'TnA_GC
Cuts at: 0 478 497 635 824 830 1119
Size: 478 19 138 189 6 289
Fragments arranged by size:
478 289 189 138 19 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 75 1119
Size: 75 1044
BsaHI Gr'CG_yC
Cuts at: 0 203 1119
Size: 203 916
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 160 190 1119
Size: 160 30 929
Fragments arranged by size:
929 160 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 276 514 856 988 1119
Size: 276 238 342 132 131
Fragments arranged by size:
342 276 238 132 131
BseYI C'CCAG_C
Cuts at: 0 265 274 357 400 504 1016 1103 1119
Size: 265 9 83 43 104 512 87 16
Fragments arranged by size:
512 265 104 87 83 43 16 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 438 505 706 784 1119
Size: 169 269 67 201 78 335
Fragments arranged by size:
335 269 201 169 78 67
BsiHKAI G_wGCw'C
Cuts at: 0 479 860 1119
Size: 479 381 259
Fragments arranged by size:
479 381 259
BsmI GAATG_Cn'
Cuts at: 0 498 1119
Size: 498 621
Bsp1286I G_dGCh'C
Cuts at: 0 266 371 479 648 689 795 860 937
Size: 266 105 108 169 41 106 65 77
Cuts at: 937 955 1017 1119
Size: 18 62 102
Fragments arranged by size:
266 169 108 106 105 102 77 65
62 41 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 223 470 881 1119
Size: 94 129 247 411 238
Fragments arranged by size:
411 247 238 129 94
BsrBI CCG'CTC
Cuts at: 0 171 747 1119
Size: 171 576 372
Fragments arranged by size:
576 372 171
BstAPI GCAn_nnn'nTGC
Cuts at: 0 180 534 589 1075 1119
Size: 180 354 55 486 44
Fragments arranged by size:
486 354 180 55 44
Bsu36I CC'TnA_GG
Cuts at: 0 250 1119
Size: 250 869
BtgI C'CryG_G
Cuts at: 0 20 346 919 1119
Size: 20 326 573 200
Fragments arranged by size:
573 326 200 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 87 248 1119
Size: 87 161 871
Fragments arranged by size:
871 161 87
BtsI GCAGTG_nn'
Cuts at: 0 596 1119
Size: 596 523
DraIII CAC_nnn'GTG
Cuts at: 0 589 1119
Size: 589 530
EaeI y'GGCC_r
Cuts at: 0 17 132 593 1119
Size: 17 115 461 526
Fragments arranged by size:
526 461 115 17
EcoICRI GAG'CTC
Cuts at: 0 477 858 1119
Size: 477 381 261
Fragments arranged by size:
477 381 261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 493 670 817 859 947 1078 1119
Size: 49 444 177 147 42 88 131 41
Fragments arranged by size:
444 177 147 131 88 49 42 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 724 1119
Size: 724 395
EcoO109I rG'GnC_Cy
Cuts at: 0 262 281 468 624 678 686 729 792
Size: 262 19 187 156 54 8 43 63
Cuts at: 792 933 934 951 952 1025 1119
Size: 141 1 17 1 73 94
Fragments arranged by size:
262 187 156 141 94 73 63 54
43 19 17 8 1 1
HaeII r_GCGC'y
Cuts at: 0 206 720 992 1119
Size: 206 514 272 127
Fragments arranged by size:
514 272 206 127
KasI G'GCGC_C
Cuts at: 0 202 1119
Size: 202 917
MslI CAynn'nnrTG
Cuts at: 0 189 1010 1119
Size: 189 821 109
Fragments arranged by size:
821 189 109
MspA1I CmG'CkG
Cuts at: 0 153 296 666 673 1119
Size: 153 143 370 7 446
Fragments arranged by size:
446 370 153 143 7
NarI GG'CG_CC
Cuts at: 0 203 1119
Size: 203 916
NcoI C'CATG_G
Cuts at: 0 20 1119
Size: 20 1099
NspI r_CATG'y
Cuts at: 0 610 1119
Size: 610 509
PasI CC'CwG_GG
Cuts at: 0 415 682 1119
Size: 415 267 437
Fragments arranged by size:
437 415 267
PflMI CCAn_nnn'nTGG
Cuts at: 0 57 96 732 1119
Size: 57 39 636 387
Fragments arranged by size:
636 387 57 39
PfoI T'CCnGG_A
Cuts at: 0 1055 1119
Size: 1055 64
PpuMI rG'GwC_Cy
Cuts at: 0 729 1119
Size: 729 390
PshAI GACnn'nnGTC
Cuts at: 0 296 1119
Size: 296 823
PspOMI G'GGCC_C
Cuts at: 0 262 685 791 933 951 1119
Size: 262 423 106 142 18 168
Fragments arranged by size:
423 262 168 142 106 18
PstI C_TGCA'G
Cuts at: 0 581 623 665 878 1119
Size: 581 42 42 213 241
Fragments arranged by size:
581 241 213 42 42
PvuII CAG'CTG
Cuts at: 0 296 666 1119
Size: 296 370 453
Fragments arranged by size:
453 370 296
SacI G_AGCT'C
Cuts at: 0 479 860 1119
Size: 479 381 259
Fragments arranged by size:
479 381 259
SbfI CC_TGCA'GG
Cuts at: 0 581 1119
Size: 581 538
SfcI C'TryA_G
Cuts at: 0 577 619 661 874 1119
Size: 577 42 42 213 245
Fragments arranged by size:
577 245 213 42 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 131 1119
Size: 131 988
SfoI GGC'GCC
Cuts at: 0 204 1119
Size: 204 915
SmaI CCC'GGG
Cuts at: 0 553 1119
Size: 553 566
SphI G_CATG'C
Cuts at: 0 610 1119
Size: 610 509
StuI AGG'CCT
Cuts at: 0 517 1119
Size: 517 602
StyI C'CwwG_G
Cuts at: 0 20 1021 1119
Size: 20 1001 98
Fragments arranged by size:
1001 98 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 27 453 1119
Size: 27 426 666
Fragments arranged by size:
666 426 27
XmaI C'CCGG_G
Cuts at: 0 551 1119
Size: 551 568
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaI AvaI AvrII
BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BstAPI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I
HaeII KasI MslI MspA1I NarI NcoI NspI PasI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI
SbfI SfcI SfiI SfoI SmaI SphI StuI StyI
TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BbsI BciVI BclI BfrBI BglII BmgBI BmrI
BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstYI BstZ17I ClaI CspCI DraI
DrdI EagI EarI EciI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI