(Circular) (Six-base) MAPSORT of: pFN21AB3501.seq Check: 902 from: 1 to: 5845 pFN21AB3501 5845 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3655 278 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3188 1937 Size: 1251 4594 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2219 2303 2745 3908 5292 5560 1632 Size: 587 84 442 1163 1384 268 1917 Fragments arranged by size: 1917 1384 1163 587 442 268 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 2094 2163 2854 4744 5721 1203 Size: 891 69 691 1890 977 1327 Fragments arranged by size: 1890 1327 977 891 691 69 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 4574 4574 Size: 5845 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5845 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5845 AlwNI CAG_nnn'CTG Cuts at: 2768 2868 3091 3588 5160 2768 Size: 100 223 497 1572 3453 Fragments arranged by size: 3453 1572 497 223 100 ApaLI G'TGCA_C Cuts at: 3902 5058 3902 Size: 1156 4689 ApoI r'AATT_y Cuts at: 1493 2233 2276 2313 2341 3038 3154 3380 3532 Size: 740 43 37 28 697 116 226 152 Cuts at: 3532 5641 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 740 697 226 152 116 43 37 28 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2143 3170 1870 Size: 73 82 118 1027 4545 Fragments arranged by size: 4545 1027 118 82 73 BamHI G'GATC_C Cuts at: 2511 2736 3175 3542 2511 Size: 225 439 367 4814 Fragments arranged by size: 4814 439 367 225 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2149 3166 4621 618 Size: 325 217 627 362 1017 1455 1842 Fragments arranged by size: 1842 1455 1017 627 362 325 217 BanII G_rGCy'C Cuts at: 1391 3117 3164 1391 Size: 1726 47 4072 Fragments arranged by size: 4072 1726 47 BbeI G_GCGC'C Cuts at: 2153 2153 Size: 5845 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4874 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4036 4070 1903 Size: 34 2099 34 3678 Fragments arranged by size: 3678 2099 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3737 4947 3737 Size: 1210 4635 BclI T'GATC_A Cuts at: 1548 1548 Size: 5845 BfrBI ATG'CAT Cuts at: 3103 3103 Size: 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4456 136 Size: 107 122 71 631 605 2784 1525 Fragments arranged by size: 2784 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3001 5840 3001 Size: 2839 3006 BlpI GC'TnA_GC Cuts at: 2057 2900 3262 2057 Size: 843 362 4640 Fragments arranged by size: 4640 843 362 Bme1580I G_kGCm'C Cuts at: 1790 2226 3906 5062 1790 Size: 436 1680 1156 2573 Fragments arranged by size: 2573 1680 1156 436 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3576 4534 442 Size: 416 53 83 399 24 2159 958 1753 Fragments arranged by size: 2159 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4505 1239 Size: 3266 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3970 4835 5133 3970 Size: 865 298 4682 Fragments arranged by size: 4682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4508 882 Size: 617 3009 2219 Fragments arranged by size: 3009 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2095 3636 493 Size: 711 891 1541 2702 Fragments arranged by size: 2702 1541 891 711 BsaBI GATnn'nnATC Cuts at: 5839 5839 Size: 5845 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2150 3652 4034 4669 Size: 53 83 186 936 617 1502 382 635 Cuts at: 4669 275 Size: 1451 Fragments arranged by size: 1502 1451 936 635 617 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2181 2436 4278 4676 4950 5097 5508 2181 Size: 255 1842 398 274 147 411 2518 Fragments arranged by size: 2518 1842 411 398 274 255 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2515 2545 1178 Size: 30 165 30 1112 30 4478 Fragments arranged by size: 4478 1112 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5845 BseYI C'CCAG_C Cuts at: 2041 2639 5048 5544 2041 Size: 598 2409 496 2342 Fragments arranged by size: 2409 2342 598 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3295 4056 5084 1540 Size: 275 186 1294 761 1028 2301 Fragments arranged by size: 2301 1294 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2906 3906 3991 5062 2906 Size: 1000 85 1071 3689 Fragments arranged by size: 3689 1071 1000 85 BsmI GAATG_Cn' Cuts at: 3105 3356 3449 3105 Size: 251 93 5501 Fragments arranged by size: 5501 251 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2906 3117 3164 3906 3991 5062 Size: 399 436 680 211 47 742 85 1071 Cuts at: 5062 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 680 436 399 211 85 47 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 3734 5464 3734 Size: 1730 4115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3200 844 Size: 2356 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3732 1394 Size: 429 1909 3507 Fragments arranged by size: 3507 1909 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4340 4514 65 Size: 1183 3092 174 1396 Fragments arranged by size: 3092 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4489 4676 1700 Size: 110 2679 187 2869 Fragments arranged by size: 2869 2679 187 110 BsrGI T'GTAC_A Cuts at: 96 2160 2919 96 Size: 2064 759 3022 Fragments arranged by size: 3022 2064 759 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5845 BssSI C'ACGA_G Cuts at: 3905 4917 3905 Size: 1012 4833 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5845 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5845 BstXI CCAn_nnnn'nTGG Cuts at: 2041 2647 2960 2041 Size: 606 313 4926 Fragments arranged by size: 4926 606 313 BstYI r'GATC_y Cuts at: 1972 2511 2736 2949 3001 3175 3542 3927 3944 Size: 539 225 213 52 174 367 385 17 Cuts at: 3944 5396 5482 5494 5840 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 539 385 367 346 225 213 174 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5293 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5106 Fragments arranged by size: 5106 683 56 BtsI GCAGTG_nn' Cuts at: 3361 4155 4175 3361 Size: 794 20 5031 Fragments arranged by size: 5031 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 2709 3149 2709 Size: 440 5405 DraIII CAC_nnn'GTG Cuts at: 3051 3140 3051 Size: 89 5756 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4852 809 Size: 507 3536 1802 Fragments arranged by size: 3536 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2834 2969 3292 Size: 54 1425 50 275 18 1004 135 323 Cuts at: 3292 4181 5810 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1004 889 323 275 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3292 1537 Size: 275 1480 4090 Fragments arranged by size: 4090 1480 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2940 3775 1353 Size: 1587 835 3423 Fragments arranged by size: 3423 1587 835 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4432 4806 4952 387 Size: 1064 138 2843 374 146 1280 Fragments arranged by size: 2843 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2303 2745 3908 4505 5292 5560 Size: 393 587 84 442 1163 597 787 268 Cuts at: 5560 1239 Size: 1524 Fragments arranged by size: 1524 1163 787 597 587 442 393 268 84 EcoNI CCTnn'n_nnAGG Cuts at: 2105 2105 Size: 5845 EcoRI G'AATT_C Cuts at: 2233 3154 3532 2233 Size: 921 378 4546 Fragments arranged by size: 4546 921 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2333 2333 Size: 5845 FspI TGC'GCA Cuts at: 4351 4351 Size: 5845 HaeII r_GCGC'y Cuts at: 1678 2153 4992 1678 Size: 475 2839 2531 Fragments arranged by size: 2839 2531 475 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4486 4518 4560 4592 1373 Size: 32 135 32 2914 32 42 32 2626 Fragments arranged by size: 2914 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2788 2860 3189 3435 669 Size: 988 281 850 72 329 246 3079 Fragments arranged by size: 3079 988 850 329 281 246 72 HindIII A'AGCT_T Cuts at: 748 2714 748 Size: 1966 3879 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KasI G'GCGC_C Cuts at: 2149 2149 Size: 5845 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2479 2587 2848 4959 5143 1305 Size: 1174 108 261 2111 184 2007 Fragments arranged by size: 2111 2007 1174 261 184 108 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4023 Fragments arranged by size: 4023 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2328 2409 2958 3018 3803 4162 4321 Size: 648 1162 81 549 60 785 359 159 Cuts at: 4321 518 Size: 2042 Fragments arranged by size: 2042 1162 785 648 549 359 159 81 60 MspA1I CmG'CkG Cuts at: 1606 3070 3088 3261 3938 5086 5331 1606 Size: 1464 18 173 677 1148 245 2120 Fragments arranged by size: 2120 1464 1148 677 245 173 18 NarI GG'CG_CC Cuts at: 2150 2150 Size: 5845 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5293 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NsiI A_TGCA'T Cuts at: 3105 3105 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 2167 2327 2858 3203 4748 1132 Size: 97 938 160 531 345 1545 2229 Fragments arranged by size: 2229 1545 938 531 345 160 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5845 PciI A'CATG_T Cuts at: 2163 2854 4744 2163 Size: 691 1890 3264 Fragments arranged by size: 3264 1890 691 PflMI CCAn_nnn'nTGG Cuts at: 1108 2733 3051 1108 Size: 1625 318 3902 Fragments arranged by size: 3902 1625 318 PfoI T'CCnGG_A Cuts at: 2646 2646 Size: 5845 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 2481 3415 2481 Size: 934 4911 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3025 3057 3025 Size: 32 5813 PstI C_TGCA'G Cuts at: 830 3197 830 Size: 2367 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 PvuII CAG'CTG Cuts at: 3070 3088 3070 Size: 18 5827 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SfcI C'TryA_G Cuts at: 826 1045 3193 4328 5009 5200 826 Size: 219 2148 1135 681 191 1471 Fragments arranged by size: 2148 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2151 2151 Size: 5845 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 2027 2145 3172 1872 Size: 155 118 1027 4545 Fragments arranged by size: 4545 1027 155 118 SmlI C'TyrA_G Cuts at: 820 1017 1943 3949 4850 5112 820 Size: 197 926 2006 901 262 1553 Fragments arranged by size: 2006 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 2485 2657 3769 5 Size: 47 2433 172 1112 2081 Fragments arranged by size: 2433 2081 1112 172 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5845 StyI C'CwwG_G Cuts at: 513 1065 3284 513 Size: 552 2219 3074 Fragments arranged by size: 3074 2219 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2258 2476 3872 3889 4042 4227 4672 Size: 1255 678 218 1396 17 153 185 445 Cuts at: 4672 325 Size: 1498 Fragments arranged by size: 1498 1396 1255 678 445 218 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2160 2919 4091 Size: 274 80 33 51 1443 183 759 1172 Cuts at: 4091 5803 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1172 759 274 183 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2515 2547 5805 5837 2515 Size: 32 3258 32 2523 Fragments arranged by size: 3258 2523 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3130 1316 Size: 1814 4031 XbaI T'CTAG_A Cuts at: 2206 3181 2206 Size: 975 4870 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2603 2603 Size: 5845 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 2025 2143 3170 1870 Size: 155 118 1027 4545 Fragments arranged by size: 4545 1027 155 118 XmnI GAAnn'nnTTC Cuts at: 2117 2316 3003 3974 2117 Size: 199 687 971 3988 Fragments arranged by size: 3988 971 687 199 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3653 276 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI