Restriction map for
pFN21AB3501
(Linear) (Six-base) MAPSORT of: KIBB3501.seq Check: 9649 from: 1 to: 1140
KIBB3501.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 298 740 1140
Size: 214 84 442 400
Fragments arranged by size:
442 400 214 84
AflIII A'CryG_T
Cuts at: 0 89 158 849 1140
Size: 89 69 691 291
Fragments arranged by size:
691 291 89 69
AlwNI CAG_nnn'CTG
Cuts at: 0 763 863 1086 1140
Size: 763 100 223 54
Fragments arranged by size:
763 223 100 54
ApoI r'AATT_y
Cuts at: 0 228 271 308 336 1033 1140
Size: 228 43 37 28 697 107
Fragments arranged by size:
697 228 107 43 37 28
AvaI C'yCGr_G
Cuts at: 0 20 138 1140
Size: 20 118 1002
Fragments arranged by size:
1002 118 20
BamHI G'GATC_C
Cuts at: 0 506 731 1140
Size: 506 225 409
Fragments arranged by size:
506 409 225
BanI G'GyrC_C
Cuts at: 0 144 1140
Size: 144 996
BanII G_rGCy'C
Cuts at: 0 1112 1140
Size: 1112 28
BbeI G_GCGC'C
Cuts at: 0 148 1140
Size: 148 992
BfrBI ATG'CAT
Cuts at: 0 1098 1140
Size: 1098 42
BglII A'GATC_T
Cuts at: 0 996 1140
Size: 996 144
BlpI GC'TnA_GC
Cuts at: 0 52 895 1140
Size: 52 843 245
Fragments arranged by size:
843 245 52
Bme1580I G_kGCm'C
Cuts at: 0 221 1140
Size: 221 919
BsaAI yAC'GTr
Cuts at: 0 90 1140
Size: 90 1050
BsaHI Gr'CG_yC
Cuts at: 0 145 1140
Size: 145 995
BsaWI w'CCGG_w
Cuts at: 0 176 431 1140
Size: 176 255 709
Fragments arranged by size:
709 255 176
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 510 540 1140
Size: 510 30 600
Fragments arranged by size:
600 510 30
BseYI C'CCAG_C
Cuts at: 0 36 634 1140
Size: 36 598 506
Fragments arranged by size:
598 506 36
BsiHKAI G_wGCw'C
Cuts at: 0 901 1140
Size: 901 239
BsmI GAATG_Cn'
Cuts at: 0 1100 1140
Size: 1100 40
Bsp1286I G_dGCh'C
Cuts at: 0 221 901 1112 1140
Size: 221 680 211 28
Fragments arranged by size:
680 221 211 28
BsrGI T'GTAC_A
Cuts at: 0 155 914 1140
Size: 155 759 226
Fragments arranged by size:
759 226 155
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 642 955 1140
Size: 36 606 313 185
Fragments arranged by size:
606 313 185 36
BstYI r'GATC_y
Cuts at: 0 506 731 944 996 1140
Size: 506 225 213 52 144
Fragments arranged by size:
506 225 213 144 52
DraI TTT'AAA
Cuts at: 0 704 1140
Size: 704 436
DraIII CAC_nnn'GTG
Cuts at: 0 1046 1135 1140
Size: 1046 89 5
Fragments arranged by size:
1046 89 5
EaeI y'GGCC_r
Cuts at: 0 829 964 1140
Size: 829 135 176
Fragments arranged by size:
829 176 135
EarI CTCTTCn'nnn_
Cuts at: 0 935 1140
Size: 935 205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 298 740 1140
Size: 214 84 442 400
Fragments arranged by size:
442 400 214 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 100 1140
Size: 100 1040
EcoRI G'AATT_C
Cuts at: 0 228 1140
Size: 228 912
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 328 1140
Size: 328 812
HaeII r_GCGC'y
Cuts at: 0 148 1140
Size: 148 992
HincII GTy'rAC
Cuts at: 0 783 855 1140
Size: 783 72 285
Fragments arranged by size:
783 285 72
HindIII A'AGCT_T
Cuts at: 0 709 1140
Size: 709 431
KasI G'GCGC_C
Cuts at: 0 144 1140
Size: 144 996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 582 843 1140
Size: 474 108 261 297
Fragments arranged by size:
474 297 261 108
MslI CAynn'nnrTG
Cuts at: 0 323 404 953 1013 1140
Size: 323 81 549 60 127
Fragments arranged by size:
549 323 127 81 60
MspA1I CmG'CkG
Cuts at: 0 1065 1083 1140
Size: 1065 18 57
Fragments arranged by size:
1065 57 18
NarI GG'CG_CC
Cuts at: 0 145 1140
Size: 145 995
NsiI A_TGCA'T
Cuts at: 0 1100 1140
Size: 1100 40
NspI r_CATG'y
Cuts at: 0 162 322 853 1140
Size: 162 160 531 287
Fragments arranged by size:
531 287 162 160
PciI A'CATG_T
Cuts at: 0 158 849 1140
Size: 158 691 291
Fragments arranged by size:
691 291 158
PflMI CCAn_nnn'nTGG
Cuts at: 0 728 1046 1140
Size: 728 318 94
Fragments arranged by size:
728 318 94
PfoI T'CCnGG_A
Cuts at: 0 641 1140
Size: 641 499
PsiI TTA'TAA
Cuts at: 0 476 1140
Size: 476 664
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1020 1052 1140
Size: 1020 32 88
Fragments arranged by size:
1020 88 32
PvuII CAG'CTG
Cuts at: 0 1065 1083 1140
Size: 1065 18 57
Fragments arranged by size:
1065 57 18
SfoI GGC'GCC
Cuts at: 0 146 1140
Size: 146 994
SmaI CCC'GGG
Cuts at: 0 22 140 1140
Size: 22 118 1000
Fragments arranged by size:
1000 118 22
SspI AAT'ATT
Cuts at: 0 480 652 1140
Size: 480 172 488
Fragments arranged by size:
488 480 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 253 471 1140
Size: 253 218 669
Fragments arranged by size:
669 253 218
TatI w'GTAC_w
Cuts at: 0 155 914 1140
Size: 155 759 226
Fragments arranged by size:
759 226 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 510 542 1140
Size: 510 32 598
Fragments arranged by size:
598 510 32
Tth111I GACn'n_nGTC
Cuts at: 0 1125 1140
Size: 1125 15
XbaI T'CTAG_A
Cuts at: 0 201 1140
Size: 201 939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 598 1140
Size: 598 542
XmaI C'CCGG_G
Cuts at: 0 20 138 1140
Size: 20 118 1002
Fragments arranged by size:
1002 118 20
XmnI GAAnn'nnTTC
Cuts at: 0 112 311 998 1140
Size: 112 199 687 142
Fragments arranged by size:
687 199 142 112
Enzymes that do cut:
AcuI AflIII AlwNI ApoI AvaI BamHI BanI BanII
BbeI BfrBI BglII BlpI Bme1580I BsaAI BsaHI BsaWI
BsaXI BseYI BsiHKAI BsmI Bsp1286I BsrGI BstXI BstYI
DraI DraIII EaeI EarI Eco57MI EcoNI EcoRI FalI
HaeII HincII HindIII KasI MmeI MslI MspA1I NarI
NsiI NspI PciI PflMI PfoI PsiI PsrI PvuII
SfoI SmaI SspI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI
BglI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BseRI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DrdI EagI EciI EcoICRI EcoO109I EcoRV FseI
FspI FspAI Hin4I HpaI KpnI MfeI MluI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI StuI StyI SwaI XhoI
ZraI