(Circular) (Six-base) MAPSORT of: pFN21AB3441.seq Check: 5843 from: 1 to: 5725 pFN21AB3441 5725 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2759 2909 2759 Size: 150 5575 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3068 1937 Size: 1131 4594 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 3024 3852 4225 1563 Size: 1461 828 373 3063 Fragments arranged by size: 3063 1461 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2607 3788 5172 5440 1632 Size: 975 1181 1384 268 1917 Fragments arranged by size: 1917 1384 1181 975 268 AflII C'TTAA_G Cuts at: 820 1017 2045 820 Size: 197 1028 4500 Fragments arranged by size: 4500 1028 197 AflIII A'CryG_T Cuts at: 1203 2498 4624 5601 1203 Size: 1295 2126 977 1327 Fragments arranged by size: 2126 1327 1295 977 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 2600 4454 2600 Size: 1854 3871 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5725 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5725 AlwNI CAG_nnn'CTG Cuts at: 3468 5040 3468 Size: 1572 4153 ApaLI G'TGCA_C Cuts at: 3782 4938 3782 Size: 1156 4569 ApoI r'AATT_y Cuts at: 1493 3034 3260 3412 5521 1493 Size: 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 3050 1870 Size: 73 1107 4545 Fragments arranged by size: 4545 1107 73 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2163 2850 3046 4501 618 Size: 325 217 627 376 687 196 1455 1842 Fragments arranged by size: 1842 1455 687 627 376 325 217 196 BanII G_rGCy'C Cuts at: 1391 2787 3044 1391 Size: 1396 257 4072 Fragments arranged by size: 4072 1396 257 BbeI G_GCGC'C Cuts at: 2854 2854 Size: 5725 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4754 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3916 3950 1903 Size: 34 1979 34 3678 Fragments arranged by size: 3678 1979 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3617 4827 3617 Size: 1210 4515 BclI T'GATC_A Cuts at: 1548 1548 Size: 5725 BfrBI ATG'CAT Cuts at: 2723 2723 Size: 5725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2915 4336 136 Size: 107 122 71 631 605 1243 1421 1525 Fragments arranged by size: 1525 1421 1243 631 605 122 107 71 BglII A'GATC_T Cuts at: 2175 3015 5720 2175 Size: 840 2705 2180 Fragments arranged by size: 2705 2180 840 BlpI GC'TnA_GC Cuts at: 3142 3142 Size: 5725 Bme1580I G_kGCm'C Cuts at: 1790 2166 2198 2506 3786 4942 1790 Size: 376 32 308 1280 1156 2573 Fragments arranged by size: 2573 1280 1156 376 308 32 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5725 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2114 2200 3456 Size: 416 53 83 399 24 697 86 1256 Cuts at: 3456 4414 442 Size: 958 1753 Fragments arranged by size: 1753 1256 958 697 416 399 86 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2592 2592 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2188 2508 2577 4385 1239 Size: 949 320 69 1808 2579 Fragments arranged by size: 2579 1808 949 320 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2996 3850 4715 5013 2996 Size: 854 865 298 3708 Fragments arranged by size: 3708 865 854 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2513 4388 882 Size: 617 1014 1875 2219 Fragments arranged by size: 2219 1875 1014 617 BsaAI yAC'GTr Cuts at: 493 1204 2499 3516 493 Size: 711 1295 1017 2702 Fragments arranged by size: 2702 1295 1017 711 BsaBI GATnn'nnATC Cuts at: 2466 5719 2466 Size: 3253 2472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 2745 2851 3532 Size: 53 83 186 936 531 681 106 681 Cuts at: 3532 3914 4549 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 681 681 635 531 382 186 106 83 53 BsaWI w'CCGG_w Cuts at: 2546 4158 4556 4830 4977 5388 2546 Size: 1612 398 274 147 411 2883 Fragments arranged by size: 2883 1612 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2351 2381 1178 Size: 30 165 30 948 30 4522 Fragments arranged by size: 4522 948 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2161 2284 2846 1186 Size: 975 123 562 4065 Fragments arranged by size: 4065 975 562 123 BseYI C'CCAG_C Cuts at: 2864 2930 4928 5424 2864 Size: 66 1998 496 3165 Fragments arranged by size: 3165 1998 496 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2100 1163 Size: 573 364 4788 Fragments arranged by size: 4788 573 364 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2914 3175 3936 4964 1540 Size: 275 186 913 261 761 1028 2301 Fragments arranged by size: 2301 1028 913 761 275 261 186 BsiHKAI G_wGCw'C Cuts at: 2787 3786 3871 4942 2787 Size: 999 85 1071 3570 Fragments arranged by size: 3570 1071 999 85 BsmI GAATG_Cn' Cuts at: 3236 3329 3236 Size: 93 5632 BsmBI CGTCTCn'nnnn_ Cuts at: 2055 2608 2055 Size: 553 5172 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2198 2506 2787 3044 3786 3871 Size: 399 376 32 308 281 257 742 85 Cuts at: 3871 4942 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 376 308 281 257 85 32 BspEI T'CCGG_A Cuts at: 5388 5388 Size: 5725 BspHI T'CATG_A Cuts at: 2304 2565 2787 3614 5344 2304 Size: 261 222 827 1730 2685 Fragments arranged by size: 2685 1730 827 261 222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2759 2909 3080 844 Size: 1915 150 171 3489 Fragments arranged by size: 3489 1915 171 150 BsrBI CCG'CTC Cuts at: 1394 1823 2847 3612 1394 Size: 429 1024 765 3507 Fragments arranged by size: 3507 1024 765 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2725 4220 4394 65 Size: 1183 1477 1495 174 1396 Fragments arranged by size: 1495 1477 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2522 2658 4369 4556 1700 Size: 110 712 136 1711 187 2869 Fragments arranged by size: 2869 1711 712 187 136 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5725 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 2143 2388 2678 3785 4797 2143 Size: 245 290 1107 1012 3071 Fragments arranged by size: 3071 1107 1012 290 245 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5725 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 2435 1184 Size: 1251 4474 BstYI r'GATC_y Cuts at: 1972 2175 3015 3055 3422 3807 3824 5276 5362 Size: 203 840 40 367 385 17 1452 86 Cuts at: 5362 5374 5720 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 840 385 367 346 203 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5173 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4986 Fragments arranged by size: 4986 683 56 BtsI GCAGTG_nn' Cuts at: 2153 3241 4035 4055 2153 Size: 1088 794 20 3823 Fragments arranged by size: 3823 1088 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5690 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DraIII CAC_nnn'GTG Cuts at: 2499 2499 Size: 5725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2945 4732 809 Size: 507 1629 1787 1802 Fragments arranged by size: 1802 1787 1629 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2169 2524 2674 Size: 54 1425 50 275 18 339 355 150 Cuts at: 2674 3172 4061 5690 8 Size: 498 889 1629 43 Fragments arranged by size: 1629 1425 889 498 355 339 275 150 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3172 1537 Size: 275 1360 4090 Fragments arranged by size: 4090 1360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3655 1353 Size: 2302 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4312 4686 4832 387 Size: 1064 138 2723 374 146 1280 Fragments arranged by size: 2723 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2785 2785 Size: 5725 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2188 2508 2577 2607 3788 4385 5172 Size: 393 556 320 69 30 1181 597 787 Cuts at: 5172 5440 1239 Size: 268 1524 Fragments arranged by size: 1524 1181 787 597 556 393 320 268 69 30 EcoO109I rG'GnC_Cy Cuts at: 2994 2994 Size: 5725 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 FspI TGC'GCA Cuts at: 2141 4231 2141 Size: 2090 3635 FspAI rTGC'GCAy Cuts at: 2141 2141 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 2854 4872 1678 Size: 1176 2018 2531 Fragments arranged by size: 2531 2018 1176 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2586 2618 4366 4398 4440 Size: 32 135 32 1014 32 1748 32 42 Cuts at: 4440 4472 1373 Size: 32 2626 Fragments arranged by size: 2626 1748 1014 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3069 3315 669 Size: 988 281 1131 246 3079 Fragments arranged by size: 3079 1131 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5725 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KasI G'GCGC_C Cuts at: 2850 2850 Size: 5725 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 3324 3324 Size: 5725 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4839 5023 1305 Size: 3534 184 2007 Fragments arranged by size: 3534 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2171 2676 10 Size: 54 1425 343 339 505 3059 Fragments arranged by size: 3059 1425 505 343 339 54 MslI CAynn'nnrTG Cuts at: 518 1166 2981 3683 4042 4201 518 Size: 648 1815 702 359 159 2042 Fragments arranged by size: 2042 1815 702 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2952 3141 3818 4966 5211 1606 Size: 1346 189 677 1148 245 2120 Fragments arranged by size: 2120 1346 1148 677 245 189 NaeI GCC'GGC Cuts at: 2660 2660 Size: 5725 NarI GG'CG_CC Cuts at: 2851 2851 Size: 5725 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5173 NdeI CA'TA_TG Cuts at: 387 2317 387 Size: 1930 3795 NgoMIV G'CCGG_C Cuts at: 2658 2658 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NsiI A_TGCA'T Cuts at: 2725 2725 Size: 5725 NspI r_CATG'y Cuts at: 1132 1229 2079 2656 3083 4628 1132 Size: 97 850 577 427 1545 2229 Fragments arranged by size: 2229 1545 850 577 427 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 4624 4624 Size: 5725 PflMI CCAn_nnn'nTGG Cuts at: 1108 2190 2634 1108 Size: 1082 444 4199 Fragments arranged by size: 4199 1082 444 PfoI T'CCnGG_A Cuts at: 2457 2457 Size: 5725 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PmlI CAC'GTG Cuts at: 2499 2499 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3794 3826 5307 5339 3794 Size: 32 1481 32 4180 Fragments arranged by size: 4180 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2994 2994 Size: 5725 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PstI C_TGCA'G Cuts at: 830 2756 3077 830 Size: 1926 321 3478 Fragments arranged by size: 3478 1926 321 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 PvuII CAG'CTG Cuts at: 2952 2952 Size: 5725 SacI G_AGCT'C Cuts at: 2787 2787 Size: 5725 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SbfI CC_TGCA'GG Cuts at: 2756 3077 2756 Size: 321 5404 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SfcI C'TryA_G Cuts at: 826 1045 2752 3073 4208 4889 5080 826 Size: 219 1707 321 1135 681 191 1471 Fragments arranged by size: 1707 1471 1135 681 321 219 191 SfoI GGC'GCC Cuts at: 2852 2852 Size: 5725 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 3052 1872 Size: 1180 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 3011 3829 4730 4992 820 Size: 197 926 102 966 818 901 262 1553 Fragments arranged by size: 1553 966 926 901 818 262 197 102 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 3083 1132 Size: 1951 3774 SspI AAT'ATT Cuts at: 5 52 3649 5 Size: 47 3597 2081 Fragments arranged by size: 3597 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5725 StyI C'CwwG_G Cuts at: 513 1065 3164 513 Size: 552 2099 3074 Fragments arranged by size: 3074 2099 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3752 3769 3922 4107 4552 325 Size: 1255 2172 17 153 185 445 1498 Fragments arranged by size: 2172 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3971 5683 96 Size: 274 80 33 51 1443 1994 1712 138 Fragments arranged by size: 1994 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2062 2094 5685 5717 2062 Size: 32 3591 32 2070 Fragments arranged by size: 3591 2070 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2992 1316 Size: 1676 4049 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 3050 1870 Size: 1180 4545 XmnI GAAnn'nnTTC Cuts at: 3854 3854 Size: 5725 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI