(Circular) (Six-base) MAPSORT of: pFN21AB3433.seq Check: 3013 from: 1 to: 5986 pFN21AB3433 5986 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 3307 3307 Size: 5986 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2650 4049 5433 5701 1632 Size: 1018 1399 1384 268 1917 Fragments arranged by size: 1917 1399 1384 1018 268 AflII C'TTAA_G Cuts at: 820 1017 2516 820 Size: 197 1499 4290 Fragments arranged by size: 4290 1499 197 AflIII A'CryG_T Cuts at: 1203 2286 4885 5862 1203 Size: 1083 2599 977 1327 Fragments arranged by size: 2599 1327 1083 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5986 AlwNI CAG_nnn'CTG Cuts at: 3071 3230 3729 5301 3071 Size: 159 499 1572 3756 Fragments arranged by size: 3756 1572 499 159 ApaI G_GGCC'C Cuts at: 2624 2624 Size: 5986 ApaLI G'TGCA_C Cuts at: 4043 5199 4043 Size: 1156 4830 ApoI r'AATT_y Cuts at: 1493 2229 3295 3521 3673 5782 1493 Size: 736 1066 226 152 2109 1697 Fragments arranged by size: 2109 1697 1066 736 226 152 AseI AT'TA_AT Cuts at: 160 1032 2859 4540 160 Size: 872 1827 1681 1606 Fragments arranged by size: 1827 1681 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 3311 1870 Size: 73 1368 4545 Fragments arranged by size: 4545 1368 73 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2328 3307 4762 618 Size: 325 217 627 541 979 1455 1842 Fragments arranged by size: 1842 1455 979 627 541 325 217 BanII G_rGCy'C Cuts at: 1391 2624 2908 3305 1391 Size: 1233 284 397 4072 Fragments arranged by size: 4072 1233 397 284 BbeI G_GCGC'C Cuts at: 2332 2332 Size: 5986 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2498 928 Size: 971 599 4416 Fragments arranged by size: 4416 971 599 BbvCI CC'TCA_GC Cuts at: 2395 2395 Size: 5986 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4177 4211 1903 Size: 34 2240 34 3678 Fragments arranged by size: 3678 2240 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2618 3878 5088 2618 Size: 1260 1210 3516 Fragments arranged by size: 3516 1260 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4597 136 Size: 107 122 71 631 605 2925 1525 Fragments arranged by size: 2925 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2866 5981 2866 Size: 3115 2871 BlpI GC'TnA_GC Cuts at: 3403 3403 Size: 5986 Bme1580I G_kGCm'C Cuts at: 1790 2624 4047 5203 1790 Size: 834 1423 1156 2573 Fragments arranged by size: 2573 1423 1156 834 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3717 4675 442 Size: 416 53 83 399 24 2300 958 1753 Fragments arranged by size: 2300 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4646 1239 Size: 3407 2579 Bpu10I CC'TnA_GC Cuts at: 2395 2395 Size: 5986 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2807 2989 4111 4976 5274 2807 Size: 182 1122 865 298 3519 Fragments arranged by size: 3519 1122 865 298 182 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4649 882 Size: 617 3150 2219 Fragments arranged by size: 3150 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3777 493 Size: 711 2573 2702 Fragments arranged by size: 2702 2573 711 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2329 3793 4175 4810 Size: 53 83 186 936 796 1464 382 635 Cuts at: 4810 275 Size: 1451 Fragments arranged by size: 1464 1451 936 796 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2775 4419 4817 5091 5238 5649 2775 Size: 1644 398 274 147 411 3112 Fragments arranged by size: 3112 1644 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5761 Fragments arranged by size: 5761 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3281 1186 Size: 2095 3891 BseYI C'CCAG_C Cuts at: 5189 5685 5189 Size: 496 5490 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5413 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3436 4197 5225 1540 Size: 275 186 1435 761 1028 2301 Fragments arranged by size: 2301 1435 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2017 2917 4047 4132 5203 2017 Size: 900 1130 85 1071 2800 Fragments arranged by size: 2800 1130 1071 900 85 BsmI GAATG_Cn' Cuts at: 3497 3590 3497 Size: 93 5893 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2624 2908 2917 3305 4047 4132 Size: 399 227 607 284 9 388 742 85 Cuts at: 4132 5203 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 607 399 388 284 227 85 9 BspEI T'CCGG_A Cuts at: 2775 5649 2775 Size: 2874 3112 BspHI T'CATG_A Cuts at: 3875 5605 3875 Size: 1730 4256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2168 3341 844 Size: 1324 1173 3489 Fragments arranged by size: 3489 1324 1173 BsrBI CCG'CTC Cuts at: 1394 1823 2262 3873 1394 Size: 429 439 1611 3507 Fragments arranged by size: 3507 1611 439 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4481 4655 65 Size: 1183 3233 174 1396 Fragments arranged by size: 3233 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4630 4817 1700 Size: 110 2820 187 2869 Fragments arranged by size: 2869 2820 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 4046 5058 4046 Size: 1012 4974 BstAPI GCAn_nnn'nTGC Cuts at: 794 3071 794 Size: 2277 3709 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2866 2895 2928 3316 3683 4068 4085 5537 Size: 894 29 33 388 367 385 17 1452 Cuts at: 5537 5623 5635 5981 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 894 388 385 367 346 86 33 29 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4495 Fragments arranged by size: 4495 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2918 487 Size: 683 56 1692 3555 Fragments arranged by size: 3555 1692 683 56 BtsI GCAGTG_nn' Cuts at: 3502 4296 4316 3502 Size: 794 20 5172 Fragments arranged by size: 5172 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 3290 3290 Size: 5986 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2266 3433 4322 Size: 54 1425 50 275 18 436 1167 889 Cuts at: 4322 5951 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1167 889 436 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3916 1353 Size: 2563 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4573 4947 5093 387 Size: 1064 138 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2650 4049 4646 5433 5701 1239 Size: 393 1018 1399 597 787 268 1524 Fragments arranged by size: 1524 1399 1018 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3135 3135 Size: 5986 EcoO109I rG'GnC_Cy Cuts at: 2620 2620 Size: 5986 EcoRI G'AATT_C Cuts at: 2229 3295 3673 2229 Size: 1066 378 4542 Fragments arranged by size: 4542 1066 378 EcoRV GAT'ATC Cuts at: 2028 2542 2028 Size: 514 5472 FspI TGC'GCA Cuts at: 2138 4492 2138 Size: 2354 3632 HaeII r_GCGC'y Cuts at: 1678 2332 5133 1678 Size: 654 2801 2531 Fragments arranged by size: 2801 2531 654 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2358 2390 2584 2616 4627 Size: 32 135 32 786 32 194 32 2011 Cuts at: 4627 4659 4701 4733 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2011 786 194 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3330 3576 669 Size: 988 281 1392 246 3079 Fragments arranged by size: 3079 1392 988 281 246 HindIII A'AGCT_T Cuts at: 748 2720 748 Size: 1972 4014 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KasI G'GCGC_C Cuts at: 2328 2328 Size: 5986 KpnI G_GTAC'C Cuts at: 3311 3311 Size: 5986 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5100 5284 1305 Size: 3795 184 2007 Fragments arranged by size: 3795 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2268 10 Size: 54 1425 343 436 3728 Fragments arranged by size: 3728 1425 436 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3944 4303 4462 518 Size: 648 2778 359 159 2042 Fragments arranged by size: 2778 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3068 3080 3402 4079 5227 5472 1606 Size: 1462 12 322 677 1148 245 2120 Fragments arranged by size: 2120 1462 1148 677 322 245 12 NarI GG'CG_CC Cuts at: 2329 2329 Size: 5986 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4495 Fragments arranged by size: 4495 939 552 NdeI CA'TA_TG Cuts at: 387 2995 387 Size: 2608 3378 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 2290 2741 3344 4889 1132 Size: 97 1061 451 603 1545 2229 Fragments arranged by size: 2229 1545 1061 603 451 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5986 PciI A'CATG_T Cuts at: 2286 4885 2286 Size: 2599 3387 PflMI CCAn_nnn'nTGG Cuts at: 1108 2325 1108 Size: 1217 4769 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PspOMI G'GGCC_C Cuts at: 2620 2620 Size: 5986 PstI C_TGCA'G Cuts at: 830 3076 3247 3286 3338 830 Size: 2246 171 39 52 3478 Fragments arranged by size: 3478 2246 171 52 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 PvuII CAG'CTG Cuts at: 3068 3068 Size: 5986 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SfcI C'TryA_G Cuts at: 826 1045 3072 3243 3261 3282 3334 4469 5150 Size: 219 2027 171 18 21 52 1135 681 Cuts at: 5150 5341 826 Size: 191 1471 Fragments arranged by size: 2027 1471 1135 681 219 191 171 52 21 18 SfoI GGC'GCC Cuts at: 2330 2330 Size: 5986 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 3313 1872 Size: 1441 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2516 2822 2968 4090 4991 5253 Size: 197 926 573 306 146 1122 901 262 Cuts at: 5253 820 Size: 1553 Fragments arranged by size: 1553 1122 926 901 573 306 262 197 146 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 2189 152 Size: 2037 3949 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 2663 1517 Size: 1146 4840 StyI C'CwwG_G Cuts at: 513 1065 2004 2496 3425 513 Size: 552 939 492 929 3074 Fragments arranged by size: 3074 939 929 552 492 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4013 4030 4183 4368 4813 325 Size: 1255 2433 17 153 185 445 1498 Fragments arranged by size: 2433 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2034 4232 5944 Size: 274 80 33 51 1443 57 2198 1712 Cuts at: 5944 96 Size: 138 Fragments arranged by size: 2198 1712 1443 274 138 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2752 2784 5946 5978 2752 Size: 32 3162 32 2760 Fragments arranged by size: 3162 2760 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5986 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2276 2322 2276 Size: 46 5940 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 3311 1870 Size: 1441 4545 XmnI GAAnn'nnTTC Cuts at: 4115 4115 Size: 5986 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI