(Circular) (Six-base) MAPSORT of: pFN21AB3406.seq Check: 4870 from: 1 to: 5806 pFN21AB3406 5806 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3616 278 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3149 1937 Size: 1212 4594 Acc65I G'GTAC_C Cuts at: 3127 3127 Size: 5806 AclI AA'CG_TT Cuts at: 1563 3933 4306 1563 Size: 2370 373 3063 Fragments arranged by size: 3063 2370 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3869 5253 5521 1632 Size: 2237 1384 268 1917 Fragments arranged by size: 2237 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2096 820 Size: 197 1079 4530 Fragments arranged by size: 4530 1079 197 AflIII A'CryG_T Cuts at: 1203 4705 5682 1203 Size: 3502 977 1327 Fragments arranged by size: 3502 1327 977 AgeI A'CCGG_T Cuts at: 4637 4637 Size: 5806 AhdI GACnn_n'nnGTC Cuts at: 4535 4535 Size: 5806 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5806 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5806 AlwNI CAG_nnn'CTG Cuts at: 2350 3549 5121 2350 Size: 1199 1572 3035 Fragments arranged by size: 3035 1572 1199 ApaLI G'TGCA_C Cuts at: 3863 5019 3863 Size: 1156 4650 ApoI r'AATT_y Cuts at: 1493 2288 2833 2883 2999 3115 3341 3493 5602 Size: 795 545 50 116 116 226 152 2109 Cuts at: 5602 1493 Size: 1697 Fragments arranged by size: 2109 1697 795 545 226 152 116 116 50 AseI AT'TA_AT Cuts at: 160 1032 2571 4360 160 Size: 872 1539 1789 1606 Fragments arranged by size: 1789 1606 1539 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 AvaI C'yCGr_G Cuts at: 1870 1943 3131 1870 Size: 73 1188 4545 Fragments arranged by size: 4545 1188 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2317 2350 2317 Size: 33 5773 BamHI G'GATC_C Cuts at: 3136 3503 3136 Size: 367 5439 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2333 2375 2794 3127 4582 Size: 325 217 627 546 42 419 333 1455 Cuts at: 4582 618 Size: 1842 Fragments arranged by size: 1842 1455 627 546 419 333 325 217 42 BanII G_rGCy'C Cuts at: 1391 3125 1391 Size: 1734 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4835 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3997 4031 1903 Size: 34 2060 34 3678 Fragments arranged by size: 3678 2060 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3698 4908 3698 Size: 1210 4596 BclI T'GATC_A Cuts at: 1548 1548 Size: 5806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4417 136 Size: 107 122 71 631 605 2745 1525 Fragments arranged by size: 2745 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2856 5801 2856 Size: 2945 2861 BlpI GC'TnA_GC Cuts at: 2112 3223 2112 Size: 1111 4695 Bme1580I G_kGCm'C Cuts at: 1790 3867 5023 1790 Size: 2077 1156 2573 Fragments arranged by size: 2573 2077 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5806 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2921 3537 4495 Size: 416 53 83 399 24 1504 616 958 Cuts at: 4495 442 Size: 1753 Fragments arranged by size: 1753 1504 958 616 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5806 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2929 4466 1239 Size: 1690 1537 2579 Fragments arranged by size: 2579 1690 1537 Bpu10I CC'TnA_GC Cuts at: 2017 2017 Size: 5806 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3931 4796 5094 3931 Size: 865 298 4643 Fragments arranged by size: 4643 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3029 4469 882 Size: 617 1530 1440 2219 Fragments arranged by size: 2219 1530 1440 617 BsaAI yAC'GTr Cuts at: 493 1204 2669 3597 493 Size: 711 1465 928 2702 Fragments arranged by size: 2702 1465 928 711 BsaBI GATnn'nnATC Cuts at: 5800 5800 Size: 5806 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3613 3995 4630 275 Size: 53 83 186 936 2080 382 635 1451 Fragments arranged by size: 2080 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4239 4637 4911 5058 5469 4239 Size: 398 274 147 411 4576 Fragments arranged by size: 4576 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2747 2777 1178 Size: 30 165 30 1344 30 4207 Fragments arranged by size: 4207 1344 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5806 BseYI C'CCAG_C Cuts at: 5009 5505 5009 Size: 496 5310 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5233 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3256 4017 5045 1540 Size: 275 186 1255 761 1028 2301 Fragments arranged by size: 2301 1255 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3867 3952 5023 3867 Size: 85 1071 4650 Fragments arranged by size: 4650 1071 85 BsmI GAATG_Cn' Cuts at: 2511 2625 3317 3410 2511 Size: 114 692 93 4907 Fragments arranged by size: 4907 692 114 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3125 3867 3952 5023 1391 Size: 399 1335 742 85 1071 2174 Fragments arranged by size: 2174 1335 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5469 5469 Size: 5806 BspHI T'CATG_A Cuts at: 3695 5425 3695 Size: 1730 4076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3161 844 Size: 2317 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3693 1394 Size: 429 1870 3507 Fragments arranged by size: 3507 1870 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2409 4301 4475 65 Size: 1183 1161 1892 174 1396 Fragments arranged by size: 1892 1396 1183 1161 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4450 4637 1700 Size: 110 2640 187 2869 Fragments arranged by size: 2869 2640 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5806 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5806 BssSI C'ACGA_G Cuts at: 3866 4878 3866 Size: 1012 4794 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5806 BstBI TT'CG_AA Cuts at: 4610 4610 Size: 5806 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5806 BstYI r'GATC_y Cuts at: 1972 2340 2353 2856 3136 3503 3888 3905 5357 Size: 368 13 503 280 367 385 17 1452 Cuts at: 5357 5443 5455 5801 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 503 385 368 367 346 280 86 17 13 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5254 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5067 Fragments arranged by size: 5067 683 56 BtsI GCAGTG_nn' Cuts at: 2240 2421 3322 4116 4136 2240 Size: 181 901 794 20 3910 Fragments arranged by size: 3910 901 794 181 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5806 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5771 DraI TTT'AAA Cuts at: 3004 3110 3004 Size: 106 5700 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4813 809 Size: 507 3497 1802 Fragments arranged by size: 3497 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 3253 4142 Size: 54 1425 50 275 18 351 1072 889 Cuts at: 4142 5771 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1072 889 351 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3253 1537 Size: 275 1441 4090 Fragments arranged by size: 4090 1441 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2615 3736 1353 Size: 1262 1121 3423 Fragments arranged by size: 3423 1262 1121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4393 4767 4913 387 Size: 1064 138 2804 374 146 1280 Fragments arranged by size: 2804 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2929 3869 4466 5253 5521 1239 Size: 393 1297 940 597 787 268 1524 Fragments arranged by size: 1524 1297 940 787 597 393 268 EcoRI G'AATT_C Cuts at: 2288 3115 3493 2288 Size: 827 378 4601 Fragments arranged by size: 4601 827 378 EcoRV GAT'ATC Cuts at: 2782 2782 Size: 5806 FspI TGC'GCA Cuts at: 4312 4312 Size: 5806 HaeII r_GCGC'y Cuts at: 1678 4953 1678 Size: 3275 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2709 2741 2747 2779 2906 Size: 32 135 32 1137 32 6 32 127 Cuts at: 2906 2938 4447 4479 4521 4553 1373 Size: 32 1509 32 42 32 2626 Fragments arranged by size: 2626 1509 1137 135 127 42 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 2238 3150 3396 669 Size: 988 281 300 912 246 3079 Fragments arranged by size: 3079 988 912 300 281 246 HindIII A'AGCT_T Cuts at: 748 2114 748 Size: 1366 4440 HpaI GTT'AAC Cuts at: 3396 3396 Size: 5806 KpnI G_GTAC'C Cuts at: 3131 3131 Size: 5806 MfeI C'AATT_G Cuts at: 3405 3405 Size: 5806 MluI A'CGCG_T Cuts at: 5682 5682 Size: 5806 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3051 4920 5104 1305 Size: 1746 1869 184 2007 Fragments arranged by size: 2007 1869 1746 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2183 10 Size: 54 1425 343 351 3633 Fragments arranged by size: 3633 1425 351 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 2699 3764 4123 4282 518 Size: 648 1248 285 1065 359 159 2042 Fragments arranged by size: 2042 1248 1065 648 359 285 159 MspA1I CmG'CkG Cuts at: 1606 2684 2829 3222 3899 5047 5292 1606 Size: 1078 145 393 677 1148 245 2120 Fragments arranged by size: 2120 1148 1078 677 393 245 145 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5254 NdeI CA'TA_TG Cuts at: 387 387 Size: 5806 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5806 NotI GC'GGCC_GC Cuts at: 3253 3253 Size: 5806 NspI r_CATG'y Cuts at: 1132 1229 3164 4709 1132 Size: 97 1935 1545 2229 Fragments arranged by size: 2229 1935 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5806 PciI A'CATG_T Cuts at: 4705 4705 Size: 5806 PflMI CCAn_nnn'nTGG Cuts at: 1108 2144 2350 3070 1108 Size: 1036 206 720 3844 Fragments arranged by size: 3844 1036 720 206 PfoI T'CCnGG_A Cuts at: 2161 2349 2945 2161 Size: 188 596 5022 Fragments arranged by size: 5022 596 188 PmeI GTTT'AAAC Cuts at: 3110 3110 Size: 5806 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3875 3907 5388 5420 3875 Size: 32 1481 32 4261 Fragments arranged by size: 4261 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5806 PsiI TTA'TAA Cuts at: 3376 3376 Size: 5806 PstI C_TGCA'G Cuts at: 830 3158 830 Size: 2328 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5806 PvuII CAG'CTG Cuts at: 2684 2684 Size: 5806 SalI G'TCGA_C Cuts at: 1936 3148 1936 Size: 1212 4594 SapI GCTCTTCn'nnn_ Cuts at: 2615 2615 Size: 5806 SbfI CC_TGCA'GG Cuts at: 3158 3158 Size: 5806 ScaI AGT'ACT Cuts at: 4054 5766 4054 Size: 1712 4094 SfcI C'TryA_G Cuts at: 826 1045 2679 3154 4289 4970 5161 826 Size: 219 1634 475 1135 681 191 1471 Fragments arranged by size: 1634 1471 1135 681 475 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5806 SmaI CCC'GGG Cuts at: 1872 3133 1872 Size: 1261 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 3910 4811 5073 820 Size: 197 926 153 1814 901 262 1553 Fragments arranged by size: 1814 1553 926 901 262 197 153 SnaBI TAC'GTA Cuts at: 493 2669 3597 493 Size: 2176 928 2702 Fragments arranged by size: 2702 2176 928 SpeI A'CTAG_T Cuts at: 152 2875 152 Size: 2723 3083 SphI G_CATG'C Cuts at: 1132 3164 1132 Size: 2032 3774 SspI AAT'ATT Cuts at: 5 52 3730 5 Size: 47 3678 2081 Fragments arranged by size: 3678 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5806 StyI C'CwwG_G Cuts at: 513 1065 2379 3245 513 Size: 552 1314 866 3074 Fragments arranged by size: 3074 1314 866 552 SwaI ATTT'AAAT Cuts at: 3004 3004 Size: 5806 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3833 3850 4003 4188 4633 325 Size: 1255 2253 17 153 185 445 1498 Fragments arranged by size: 2253 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4052 5764 96 Size: 274 80 33 51 1443 2075 1712 138 Fragments arranged by size: 2075 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5766 5798 5766 Size: 32 5774 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5806 XbaI T'CTAG_A Cuts at: 3142 3142 Size: 5806 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2773 2953 2773 Size: 180 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5806 XmaI C'CCGG_G Cuts at: 1870 3131 1870 Size: 1261 4545 XmnI GAAnn'nnTTC Cuts at: 3935 3935 Size: 5806 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3614 276 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI