(Circular) (Six-base) MAPSORT of: pFN21AB3336.seq Check: 9495 from: 1 to: 6358 pFN21AB3336 6358 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2904 4168 278 Size: 53 83 186 936 1368 1264 2468 Fragments arranged by size: 2468 1368 1264 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3701 1937 Size: 1764 4594 Acc65I G'GTAC_C Cuts at: 3679 3679 Size: 6358 AclI AA'CG_TT Cuts at: 1563 4485 4858 1563 Size: 2922 373 3063 Fragments arranged by size: 3063 2922 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2674 2846 2858 2926 4421 5805 6073 1632 Size: 1042 172 12 68 1495 1384 268 1917 Fragments arranged by size: 1917 1495 1384 1042 268 172 68 12 AflII C'TTAA_G Cuts at: 820 1017 3049 820 Size: 197 2032 4129 Fragments arranged by size: 4129 2032 197 AflIII A'CryG_T Cuts at: 1203 5257 6234 1203 Size: 4054 977 1327 Fragments arranged by size: 4054 1327 977 AgeI A'CCGG_T Cuts at: 5189 5189 Size: 6358 AhdI GACnn_n'nnGTC Cuts at: 5087 5087 Size: 6358 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6358 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6358 AlwNI CAG_nnn'CTG Cuts at: 2296 2683 3235 3402 3418 4101 5673 2296 Size: 387 552 167 16 683 1572 2981 Fragments arranged by size: 2981 1572 683 552 387 167 16 ApaI G_GGCC'C Cuts at: 3577 3577 Size: 6358 ApaLI G'TGCA_C Cuts at: 4415 5571 4415 Size: 1156 5202 ApoI r'AATT_y Cuts at: 1493 3667 3893 4045 6154 1493 Size: 2174 226 152 2109 1697 Fragments arranged by size: 2174 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4912 160 Size: 872 3880 1606 Fragments arranged by size: 3880 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 AvaI C'yCGr_G Cuts at: 1870 1943 2122 3683 1870 Size: 73 179 1561 4545 Fragments arranged by size: 4545 1561 179 73 BamHI G'GATC_C Cuts at: 3688 4055 3688 Size: 367 5991 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2959 3031 3199 3679 5134 Size: 325 217 627 1172 72 168 480 1455 Cuts at: 5134 618 Size: 1842 Fragments arranged by size: 1842 1455 1172 627 480 325 217 168 72 BanII G_rGCy'C Cuts at: 1391 2164 2281 2669 2885 3042 3577 3677 1391 Size: 773 117 388 216 157 535 100 4072 Fragments arranged by size: 4072 773 535 388 216 157 117 100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3180 3204 928 Size: 971 1281 24 4082 Fragments arranged by size: 4082 1281 971 24 BbvCI CC'TCA_GC Cuts at: 2756 2756 Size: 6358 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4549 4583 1903 Size: 34 2612 34 3678 Fragments arranged by size: 3678 2612 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4250 5460 4250 Size: 1210 5148 BclI T'GATC_A Cuts at: 1548 2796 1548 Size: 1248 5110 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2952 3421 Size: 107 122 71 631 605 337 943 469 Cuts at: 3421 3556 4969 136 Size: 135 1413 1525 Fragments arranged by size: 1525 1413 943 631 605 469 337 135 122 107 71 BglII A'GATC_T Cuts at: 2967 6353 2967 Size: 3386 2972 BlpI GC'TnA_GC Cuts at: 2879 3775 2879 Size: 896 5462 Bme1580I G_kGCm'C Cuts at: 1790 3202 3577 4419 5575 1790 Size: 1412 375 842 1156 2573 Fragments arranged by size: 2573 1412 1156 842 375 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6358 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2095 2605 3294 Size: 416 53 83 399 24 678 510 689 Cuts at: 3294 3412 4089 5047 442 Size: 118 677 958 1753 Fragments arranged by size: 1753 958 689 678 677 510 416 399 118 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6358 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2167 2519 2609 2683 2692 3008 3256 3614 Size: 928 352 90 74 9 316 248 358 Cuts at: 3614 5018 1239 Size: 1404 2579 Fragments arranged by size: 2579 1404 928 358 352 316 248 90 74 9 Bpu10I CC'TnA_GC Cuts at: 2756 3036 3471 2756 Size: 280 435 5643 Fragments arranged by size: 5643 435 280 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2546 4483 5348 5646 2546 Size: 1937 865 298 3258 Fragments arranged by size: 3258 1937 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2429 5021 882 Size: 617 930 2592 2219 Fragments arranged by size: 2592 2219 930 617 BsaAI yAC'GTr Cuts at: 493 1204 2431 4149 493 Size: 711 1227 1718 2702 Fragments arranged by size: 2702 1718 1227 711 BsaBI GATnn'nnATC Cuts at: 2363 6352 2363 Size: 3989 2369 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2029 2901 3252 4165 Size: 53 83 186 936 496 872 351 913 Cuts at: 4165 4547 5182 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 913 872 635 496 382 351 186 83 53 BsaWI w'CCGG_w Cuts at: 4791 5189 5463 5610 6021 4791 Size: 398 274 147 411 5128 Fragments arranged by size: 5128 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2886 2916 1178 Size: 30 165 30 1483 30 4620 Fragments arranged by size: 4620 1483 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2039 2555 2735 2910 3296 3512 1186 Size: 844 9 516 180 175 386 216 4032 Fragments arranged by size: 4032 844 516 386 216 180 175 9 BseYI C'CCAG_C Cuts at: 2457 2874 3317 3411 3491 5561 6057 2457 Size: 417 443 94 80 2070 496 2758 Fragments arranged by size: 2758 2070 496 443 417 94 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3244 1163 Size: 573 1508 4277 Fragments arranged by size: 4277 1508 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3808 4569 5597 1540 Size: 275 186 1807 761 1028 2301 Fragments arranged by size: 2301 1807 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2885 4419 4504 5575 2885 Size: 1534 85 1071 3668 Fragments arranged by size: 3668 1534 1071 85 BsmI GAATG_Cn' Cuts at: 3869 3962 3869 Size: 93 6265 BsmBI CGTCTCn'nnnn_ Cuts at: 2585 2892 2585 Size: 307 6051 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2164 2281 2669 2885 3042 3202 3577 Size: 399 374 117 388 216 157 160 375 Cuts at: 3577 3677 4419 4504 5575 1391 Size: 100 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 388 375 374 216 160 157 117 100 85 BspEI T'CCGG_A Cuts at: 6021 6021 Size: 6358 BspHI T'CATG_A Cuts at: 4247 5977 4247 Size: 1730 4628 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3291 3713 844 Size: 2447 422 3489 Fragments arranged by size: 3489 2447 422 BsrBI CCG'CTC Cuts at: 1394 1823 4245 1394 Size: 429 2422 3507 Fragments arranged by size: 3507 2422 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4853 5027 65 Size: 1183 3605 174 1396 Fragments arranged by size: 3605 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3353 5002 5189 1700 Size: 110 1543 1649 187 2869 Fragments arranged by size: 2869 1649 1543 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6358 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6358 BssSI C'ACGA_G Cuts at: 2747 4418 5430 2747 Size: 1671 1012 3675 Fragments arranged by size: 3675 1671 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6358 BstBI TT'CG_AA Cuts at: 5162 5162 Size: 6358 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6358 BstYI r'GATC_y Cuts at: 1972 2580 2967 3688 4055 4440 4457 5909 5995 Size: 608 387 721 367 385 17 1452 86 Cuts at: 5995 6007 6353 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 721 608 387 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2321 2321 Size: 6358 BtgI C'CryG_G Cuts at: 513 1065 2004 2729 2819 513 Size: 552 939 725 90 4052 Fragments arranged by size: 4052 939 725 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2362 487 Size: 683 56 1136 4483 Fragments arranged by size: 4483 1136 683 56 BtsI GCAGTG_nn' Cuts at: 3330 3352 3874 4668 4688 3330 Size: 22 522 794 20 5000 Fragments arranged by size: 5000 794 522 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6358 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6323 DraI TTT'AAA Cuts at: 3662 3662 Size: 6358 DraIII CAC_nnn'GTG Cuts at: 3655 3655 Size: 6358 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5365 809 Size: 507 4049 1802 Fragments arranged by size: 4049 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2195 2361 2737 Size: 54 1425 50 275 18 365 166 376 Cuts at: 2737 3192 3805 4694 6323 8 Size: 455 613 889 1629 43 Fragments arranged by size: 1629 1425 889 613 455 376 365 275 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3805 1537 Size: 275 1993 4090 Fragments arranged by size: 4090 1993 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2923 3296 3354 4288 1353 Size: 1570 373 58 934 3423 Fragments arranged by size: 3423 1570 934 373 58 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4945 5319 5465 387 Size: 1064 138 3356 374 146 1280 Fragments arranged by size: 3356 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2883 2883 Size: 6358 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2167 2519 2609 2674 2683 2692 2846 Size: 393 535 352 90 65 9 9 154 Cuts at: 2846 2858 2926 3008 3256 3614 4421 5018 5805 Size: 12 68 82 248 358 807 597 787 Cuts at: 5805 6073 1239 Size: 268 1524 Fragments arranged by size: 1524 807 787 597 535 393 358 352 268 248 154 90 82 68 65 12 9 9 EcoNI CCTnn'n_nnAGG Cuts at: 2643 2714 3020 3237 3544 2643 Size: 71 306 217 307 5457 Fragments arranged by size: 5457 307 306 217 71 EcoO109I rG'GnC_Cy Cuts at: 2545 2833 3406 3422 3502 3573 3574 3640 2545 Size: 288 573 16 80 71 1 66 5263 Fragments arranged by size: 5263 573 288 80 71 66 16 1 EcoRI G'AATT_C Cuts at: 3667 4045 3667 Size: 378 5980 FspI TGC'GCA Cuts at: 4864 4864 Size: 6358 HaeII r_GCGC'y Cuts at: 1678 5505 1678 Size: 3827 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2118 2150 2886 2918 3029 Size: 32 135 32 546 32 736 32 111 Cuts at: 3029 3061 3149 3181 4999 5031 5073 5105 1373 Size: 32 88 32 1818 32 42 32 2626 Fragments arranged by size: 2626 1818 736 546 135 111 88 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2308 3702 3948 669 Size: 988 281 370 1394 246 3079 Fragments arranged by size: 3079 1394 988 370 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6358 HpaI GTT'AAC Cuts at: 3948 3948 Size: 6358 KpnI G_GTAC'C Cuts at: 3683 3683 Size: 6358 MfeI C'AATT_G Cuts at: 3957 3957 Size: 6358 MluI A'CGCG_T Cuts at: 6234 6234 Size: 6358 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5472 5656 1305 Size: 4167 184 2007 Fragments arranged by size: 4167 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 10 Size: 54 1425 343 531 4005 Fragments arranged by size: 4005 1425 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2783 4316 4675 4834 518 Size: 648 1617 1533 359 159 2042 Fragments arranged by size: 2042 1617 1533 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2084 2760 2878 3010 3075 3774 4451 5599 Size: 478 676 118 132 65 699 677 1148 Cuts at: 5599 5844 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 699 677 676 478 245 132 118 65 NcoI C'CATG_G Cuts at: 513 1065 2004 2819 513 Size: 552 939 815 4052 Fragments arranged by size: 4052 939 815 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6358 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6358 NotI GC'GGCC_GC Cuts at: 3805 3805 Size: 6358 NspI r_CATG'y Cuts at: 1132 1229 2112 3716 5261 1132 Size: 97 883 1604 1545 2229 Fragments arranged by size: 2229 1604 1545 883 97 PasI CC'CwG_GG Cuts at: 1348 3338 3418 3519 1348 Size: 1990 80 101 4187 Fragments arranged by size: 4187 1990 101 80 PciI A'CATG_T Cuts at: 5257 5257 Size: 6358 PflMI CCAn_nnn'nTGG Cuts at: 1108 2683 2730 3402 3418 1108 Size: 1575 47 672 16 4048 Fragments arranged by size: 4048 1575 672 47 16 PfoI T'CCnGG_A Cuts at: 2367 3111 2367 Size: 744 5614 PmeI GTTT'AAAC Cuts at: 3662 3662 Size: 6358 PmlI CAC'GTG Cuts at: 2431 2431 Size: 6358 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4427 4459 5940 5972 4427 Size: 32 1481 32 4813 Fragments arranged by size: 4813 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2833 3406 3502 3640 2833 Size: 573 96 138 5551 Fragments arranged by size: 5551 573 138 96 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6358 PsiI TTA'TAA Cuts at: 3928 3928 Size: 6358 PspOMI G'GGCC_C Cuts at: 3573 3573 Size: 6358 PstI C_TGCA'G Cuts at: 830 2083 2169 2707 2807 3021 3231 3710 830 Size: 1253 86 538 100 214 210 479 3478 Fragments arranged by size: 3478 1253 538 479 214 210 100 86 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6358 PvuII CAG'CTG Cuts at: 2878 3010 3075 2878 Size: 132 65 6161 Fragments arranged by size: 6161 132 65 SacI G_AGCT'C Cuts at: 2885 2885 Size: 6358 SalI G'TCGA_C Cuts at: 1936 3700 1936 Size: 1764 4594 SanDI GG'GwC_CC Cuts at: 3406 3406 Size: 6358 SapI GCTCTTCn'nnn_ Cuts at: 3296 3296 Size: 6358 SbfI CC_TGCA'GG Cuts at: 3710 3710 Size: 6358 ScaI AGT'ACT Cuts at: 4606 6318 4606 Size: 1712 4646 SexAI A'CCwGG_T Cuts at: 3447 3447 Size: 6358 SfcI C'TryA_G Cuts at: 826 1045 2079 2165 2703 2803 3017 3227 3706 Size: 219 1034 86 538 100 214 210 479 Cuts at: 3706 4841 5522 5713 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1034 681 538 479 219 214 210 191 100 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6358 SmaI CCC'GGG Cuts at: 1872 3685 1872 Size: 1813 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2122 2561 3049 4462 5363 5625 Size: 197 926 179 439 488 1413 901 262 Cuts at: 5625 820 Size: 1553 Fragments arranged by size: 1553 1413 926 901 488 439 262 197 179 SnaBI TAC'GTA Cuts at: 493 4149 493 Size: 3656 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6358 SphI G_CATG'C Cuts at: 1132 3716 1132 Size: 2584 3774 SspI AAT'ATT Cuts at: 5 52 4282 5 Size: 47 4230 2081 Fragments arranged by size: 4230 2081 47 StuI AGG'CCT Cuts at: 1517 3134 3550 1517 Size: 1617 416 4325 Fragments arranged by size: 4325 1617 416 StyI C'CwwG_G Cuts at: 513 1065 2004 2710 2819 3120 3797 513 Size: 552 939 706 109 301 677 3074 Fragments arranged by size: 3074 939 706 677 552 301 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2624 3217 4385 4402 4555 4740 5185 Size: 1255 1044 593 1168 17 153 185 445 Cuts at: 5185 325 Size: 1498 Fragments arranged by size: 1498 1255 1168 1044 593 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2787 3144 4604 Size: 274 80 33 51 1443 810 357 1460 Cuts at: 4604 6316 96 Size: 1712 138 Fragments arranged by size: 1712 1460 1443 810 357 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6318 6350 6318 Size: 32 6326 Tth111I GACn'n_nGTC Cuts at: 1316 2312 2518 1316 Size: 996 206 5156 Fragments arranged by size: 5156 996 206 XbaI T'CTAG_A Cuts at: 3694 3694 Size: 6358 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2532 2532 Size: 6358 XhoI C'TCGA_G Cuts at: 1943 2122 1943 Size: 179 6179 XmaI C'CCGG_G Cuts at: 1870 3683 1870 Size: 1813 4545 XmnI GAAnn'nnTTC Cuts at: 4487 4487 Size: 6358 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2902 4166 276 Size: 53 83 186 936 1368 1264 2468 Fragments arranged by size: 2468 1368 1264 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI