(Circular) (Six-base) MAPSORT of: pFN21AB1529.seq Check: 6283 from: 1 to: 8611 pFN21AB1529 8611 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2090 2090 Size: 8611 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6421 278 Size: 53 83 186 936 4885 2468 Fragments arranged by size: 4885 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4229 5954 1937 Size: 2292 1725 4594 Fragments arranged by size: 4594 2292 1725 Acc65I G'GTAC_C Cuts at: 2768 5932 2768 Size: 3164 5447 AclI AA'CG_TT Cuts at: 1563 6738 7111 1563 Size: 5175 373 3063 Fragments arranged by size: 5175 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2694 2781 3207 4231 4293 4509 5630 6674 Size: 1062 87 426 1024 62 216 1121 1044 Cuts at: 6674 8058 8326 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1121 1062 1044 1024 426 268 216 87 62 AflII C'TTAA_G Cuts at: 820 1017 4544 820 Size: 197 3527 4887 Fragments arranged by size: 4887 3527 197 AflIII A'CryG_T Cuts at: 1203 5012 7510 8487 1203 Size: 3809 2498 977 1327 Fragments arranged by size: 3809 2498 1327 977 AgeI A'CCGG_T Cuts at: 3196 7442 3196 Size: 4246 4365 AhdI GACnn_n'nnGTC Cuts at: 3263 7340 3263 Size: 4077 4534 AleI CACnn'nnGTG Cuts at: 1166 5579 1166 Size: 4413 4198 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4620 1184 Size: 3436 5175 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2597 2629 2597 Size: 32 8579 AlwNI CAG_nnn'CTG Cuts at: 2024 3230 3701 3956 4981 5608 6354 7926 2024 Size: 1206 471 255 1025 627 746 1572 2709 Fragments arranged by size: 2709 1572 1206 1025 746 627 471 255 ApaLI G'TGCA_C Cuts at: 6668 7824 6668 Size: 1156 7455 ApoI r'AATT_y Cuts at: 1493 2527 3683 4019 4352 5111 5920 6146 6298 Size: 1034 1156 336 333 759 809 226 152 Cuts at: 6298 8407 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1156 1034 809 759 336 333 226 152 AseI AT'TA_AT Cuts at: 160 1032 7165 160 Size: 872 6133 1606 Fragments arranged by size: 6133 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8611 AvaI C'yCGr_G Cuts at: 1870 1943 5770 5936 1870 Size: 73 3827 166 4545 Fragments arranged by size: 4545 3827 166 73 AvrII C'CTAG_G Cuts at: 2290 2692 2290 Size: 402 8209 BamHI G'GATC_C Cuts at: 2851 3407 4757 5941 6308 2851 Size: 556 1350 1184 367 5154 Fragments arranged by size: 5154 1350 1184 556 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2768 5036 5826 5932 7387 Size: 325 217 627 981 2268 790 106 1455 Cuts at: 7387 618 Size: 1842 Fragments arranged by size: 2268 1842 1455 981 790 627 325 217 106 BanII G_rGCy'C Cuts at: 1391 3239 5930 1391 Size: 1848 2691 4072 Fragments arranged by size: 4072 2691 1848 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4237 4293 4425 928 Size: 971 2338 56 132 5114 Fragments arranged by size: 5114 2338 971 132 56 BbvCI CC'TCA_GC Cuts at: 3248 4765 3248 Size: 1517 7094 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6802 6836 1903 Size: 34 4865 34 3678 Fragments arranged by size: 4865 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2727 2979 6503 7713 2727 Size: 252 3524 1210 3625 Fragments arranged by size: 3625 3524 1210 252 BclI T'GATC_A Cuts at: 1548 5166 1548 Size: 3618 4993 BfrBI ATG'CAT Cuts at: 5387 5387 Size: 8611 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7222 136 Size: 107 122 71 631 605 5550 1525 Fragments arranged by size: 5550 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3689 4444 4540 8606 3689 Size: 755 96 4066 3694 Fragments arranged by size: 4066 3694 755 96 BlpI GC'TnA_GC Cuts at: 2859 6028 2859 Size: 3169 5442 Bme1580I G_kGCm'C Cuts at: 1790 3490 5419 5829 6672 7828 1790 Size: 1700 1929 410 843 1156 2573 Fragments arranged by size: 2573 1929 1700 1156 843 410 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8611 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2715 6342 7300 Size: 416 53 83 399 24 1298 3627 958 Cuts at: 7300 442 Size: 1753 Fragments arranged by size: 3627 1753 1298 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8611 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5771 5771 Size: 8611 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2307 2325 2553 3621 4330 5250 5582 7271 Size: 1068 18 228 1068 709 920 332 1689 Cuts at: 7271 1239 Size: 2579 Fragments arranged by size: 2579 1689 1068 1068 920 709 332 228 18 Bpu10I CC'TnA_GC Cuts at: 3025 3248 4765 5730 3025 Size: 223 1517 965 5906 Fragments arranged by size: 5906 1517 965 223 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2103 5028 5484 6736 7601 7899 2103 Size: 2925 456 1252 865 298 2815 Fragments arranged by size: 2925 2815 1252 865 456 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2167 4871 5283 7274 882 Size: 617 668 2704 412 1991 2219 Fragments arranged by size: 2704 2219 1991 668 617 412 BsaAI yAC'GTr Cuts at: 493 1204 5013 6402 493 Size: 711 3809 1389 2702 Fragments arranged by size: 3809 2702 1389 711 BsaBI GATnn'nnATC Cuts at: 8605 8605 Size: 8611 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2425 6418 6800 7435 Size: 53 83 186 936 892 3993 382 635 Cuts at: 7435 275 Size: 1451 Fragments arranged by size: 3993 1451 936 892 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3196 7044 7442 7716 7863 8274 3196 Size: 3848 398 274 147 411 3533 Fragments arranged by size: 3848 3533 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2597 2627 2923 2953 4328 Size: 30 165 30 1194 30 296 30 1375 Cuts at: 4328 4358 4997 5027 1178 Size: 30 639 30 4762 Fragments arranged by size: 4762 1375 1194 639 296 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2621 4551 5233 5788 1186 Size: 1435 1930 682 555 4009 Fragments arranged by size: 4009 1930 1435 682 555 BseYI C'CCAG_C Cuts at: 3145 3471 4888 5018 5023 5560 7814 8310 3145 Size: 326 1417 130 5 537 2254 496 3446 Fragments arranged by size: 3446 2254 1417 537 496 326 130 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5605 1163 Size: 573 3869 4169 Fragments arranged by size: 4169 3869 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6061 6822 7850 1540 Size: 275 186 4060 761 1028 2301 Fragments arranged by size: 4060 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4673 6672 6757 7828 4673 Size: 1999 85 1071 5456 Fragments arranged by size: 5456 1999 1071 85 BsmI GAATG_Cn' Cuts at: 2286 2874 5783 6122 6215 2286 Size: 588 2909 339 93 4682 Fragments arranged by size: 4682 2909 588 339 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3239 3490 4673 5419 5829 5930 6672 Size: 399 1449 251 1183 746 410 101 742 Cuts at: 6672 6757 7828 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1449 1183 1071 746 742 410 399 251 101 85 BspEI T'CCGG_A Cuts at: 8274 8274 Size: 8611 BspHI T'CATG_A Cuts at: 6500 8230 6500 Size: 1730 6881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2090 4989 5913 5966 844 Size: 1246 2899 924 53 3489 Fragments arranged by size: 3489 2899 1246 924 53 BsrBI CCG'CTC Cuts at: 1394 1823 6498 1394 Size: 429 4675 3507 Fragments arranged by size: 4675 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3123 3524 7106 7280 65 Size: 1183 1875 401 3582 174 1396 Fragments arranged by size: 3582 1875 1396 1183 401 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3196 7255 7442 1700 Size: 110 1386 4059 187 2869 Fragments arranged by size: 4059 2869 1386 187 110 BsrGI T'GTAC_A Cuts at: 96 5535 96 Size: 5439 3172 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8611 BssSI C'ACGA_G Cuts at: 6671 7683 6671 Size: 1012 7599 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8611 BstBI TT'CG_AA Cuts at: 7415 7415 Size: 8611 BstEII G'GTnAC_C Cuts at: 1184 5542 1184 Size: 4358 4253 BstXI CCAn_nnnn'nTGG Cuts at: 2728 4186 5554 2728 Size: 1458 1368 5785 Fragments arranged by size: 5785 1458 1368 BstYI r'GATC_y Cuts at: 1972 2851 3407 3604 3689 4444 4540 4757 5941 Size: 879 556 197 85 755 96 217 1184 Cuts at: 5941 6308 6693 6710 8162 8248 8260 8606 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1184 879 755 556 385 367 346 217 197 96 86 85 17 12 Bsu36I CC'TnA_GG Cuts at: 3793 3793 Size: 8611 BtgI C'CryG_G Cuts at: 513 1065 2004 2046 513 Size: 552 939 42 7078 Fragments arranged by size: 7078 939 552 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2559 487 Size: 683 56 1333 6539 Fragments arranged by size: 6539 1333 683 56 BtsI GCAGTG_nn' Cuts at: 2473 2775 4401 4474 4671 5574 5586 5593 6127 Size: 302 1626 73 197 903 12 7 534 Cuts at: 6127 6921 6941 2473 Size: 794 20 4143 Fragments arranged by size: 4143 1626 903 794 534 302 197 73 20 12 7 ClaI AT'CG_AT Cuts at: 1554 5619 1554 Size: 4065 4546 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8576 DraI TTT'AAA Cuts at: 3135 3556 3724 4906 5915 3135 Size: 421 168 1182 1009 5831 Fragments arranged by size: 5831 1182 1009 421 168 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4225 7618 809 Size: 507 2909 3393 1802 Fragments arranged by size: 3393 2909 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2015 2680 2730 Size: 54 1425 50 275 18 185 665 50 Cuts at: 2730 4591 6058 6947 8576 8 Size: 1861 1467 889 1629 43 Fragments arranged by size: 1861 1629 1467 1425 889 665 275 185 54 50 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6058 1537 Size: 275 4246 4090 Fragments arranged by size: 4246 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2361 2430 2486 2814 4875 6541 1353 Size: 1008 69 56 328 2061 1666 3423 Fragments arranged by size: 3423 2061 1666 1008 328 69 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2056 7198 7572 7718 387 Size: 1064 138 467 5142 374 146 1280 Fragments arranged by size: 5142 1280 1064 467 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2307 2325 2553 2694 2781 3207 3621 Size: 393 675 18 228 141 87 426 414 Cuts at: 3621 4231 4293 4330 4509 5250 5582 5630 6674 Size: 610 62 37 179 741 332 48 1044 Cuts at: 6674 7271 8058 8326 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1044 787 741 675 610 597 426 414 393 332 268 228 179 141 87 62 48 37 18 EcoNI CCTnn'n_nnAGG Cuts at: 2097 2121 2289 4151 2097 Size: 24 168 1862 6557 Fragments arranged by size: 6557 1862 168 24 EcoO109I rG'GnC_Cy Cuts at: 2228 2594 3227 4978 2228 Size: 366 633 1751 5861 Fragments arranged by size: 5861 1751 633 366 EcoRI G'AATT_C Cuts at: 3683 4019 5920 6298 3683 Size: 336 1901 378 5996 Fragments arranged by size: 5996 1901 378 336 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2938 3083 4886 5271 2938 Size: 145 1803 385 6278 Fragments arranged by size: 6278 1803 385 145 FspI TGC'GCA Cuts at: 7117 7117 Size: 8611 HaeII r_GCGC'y Cuts at: 1678 2012 7758 1678 Size: 334 5746 2531 Fragments arranged by size: 5746 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3249 3281 3563 3595 3725 Size: 32 135 32 1677 32 282 32 130 Cuts at: 3725 3757 4328 4360 4412 4444 4484 4516 5666 Size: 32 571 32 52 32 40 32 1150 Cuts at: 5666 5681 5698 5713 7252 7284 7326 7358 1373 Size: 15 17 15 1539 32 42 32 2626 Fragments arranged by size: 2626 1677 1539 1150 571 282 135 130 52 42 40 32 32 32 32 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 3948 5955 6201 669 Size: 988 281 2010 2007 246 3079 Fragments arranged by size: 3079 2010 2007 988 281 246 HindIII A'AGCT_T Cuts at: 748 3673 748 Size: 2925 5686 HpaI GTT'AAC Cuts at: 6201 6201 Size: 8611 KpnI G_GTAC'C Cuts at: 2772 5936 2772 Size: 3164 5447 MfeI C'AATT_G Cuts at: 2814 6210 2814 Size: 3396 5215 MluI A'CGCG_T Cuts at: 8487 8487 Size: 8611 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2966 3185 3265 7725 7909 1305 Size: 1661 219 80 4460 184 2007 Fragments arranged by size: 4460 2007 1661 219 184 80 MscI TGG'CCA Cuts at: 10 64 1489 1832 2682 2732 4593 10 Size: 54 1425 343 850 50 1861 4028 Fragments arranged by size: 4028 1861 1425 850 343 54 50 MslI CAynn'nnrTG Cuts at: 518 1166 2978 4823 5392 5579 6569 6928 7087 Size: 648 1812 1845 569 187 990 359 159 Cuts at: 7087 518 Size: 2042 Fragments arranged by size: 2042 1845 1812 990 648 569 359 187 159 MspA1I CmG'CkG Cuts at: 1606 2048 2276 3569 3908 5844 6027 6704 7852 Size: 442 228 1293 339 1936 183 677 1148 Cuts at: 7852 8097 1606 Size: 245 2120 Fragments arranged by size: 2120 1936 1293 1148 677 442 339 245 228 183 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7120 Fragments arranged by size: 7120 939 552 NdeI CA'TA_TG Cuts at: 387 5389 387 Size: 5002 3609 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8611 NotI GC'GGCC_GC Cuts at: 6058 6058 Size: 8611 NsiI A_TGCA'T Cuts at: 5389 5389 Size: 8611 NspI r_CATG'y Cuts at: 1132 1229 2317 2350 4553 5969 7514 1132 Size: 97 1088 33 2203 1416 1545 2229 Fragments arranged by size: 2229 2203 1545 1416 1088 97 33 PasI CC'CwG_GG Cuts at: 1348 2997 4381 1348 Size: 1649 1384 5578 Fragments arranged by size: 5578 1649 1384 PciI A'CATG_T Cuts at: 7510 7510 Size: 8611 PflMI CCAn_nnn'nTGG Cuts at: 1108 2024 3296 3512 5856 1108 Size: 916 1272 216 2344 3863 Fragments arranged by size: 3863 2344 1272 916 216 PfoI T'CCnGG_A Cuts at: 3378 3511 3378 Size: 133 8478 PmeI GTTT'AAAC Cuts at: 5915 5915 Size: 8611 PmlI CAC'GTG Cuts at: 5013 5013 Size: 8611 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2597 2629 6680 6712 8193 8225 2597 Size: 32 4051 32 1481 32 2983 Fragments arranged by size: 4051 2983 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2228 3227 4978 2228 Size: 999 1751 5861 Fragments arranged by size: 5861 1751 999 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8611 PsiI TTA'TAA Cuts at: 6181 6181 Size: 8611 PspXI vC'TCGA_Gb Cuts at: 5770 5770 Size: 8611 PstI C_TGCA'G Cuts at: 830 2782 3307 3697 4612 5581 5963 830 Size: 1952 525 390 915 969 382 3478 Fragments arranged by size: 3478 1952 969 915 525 390 382 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8611 PvuII CAG'CTG Cuts at: 3569 3908 5844 3569 Size: 339 1936 6336 Fragments arranged by size: 6336 1936 339 SacII CC_GC'GG Cuts at: 2049 2049 Size: 8611 SalI G'TCGA_C Cuts at: 1936 5953 1936 Size: 4017 4594 SapI GCTCTTCn'nnn_ Cuts at: 2361 2430 4875 2361 Size: 69 2445 6097 Fragments arranged by size: 6097 2445 69 SbfI CC_TGCA'GG Cuts at: 5963 5963 Size: 8611 ScaI AGT'ACT Cuts at: 6859 8571 6859 Size: 1712 6899 SfcI C'TryA_G Cuts at: 826 1045 2748 2778 3303 3693 4608 4648 5577 Size: 219 1703 30 525 390 915 40 929 Cuts at: 5577 5959 7094 7775 7966 826 Size: 382 1135 681 191 1471 Fragments arranged by size: 1703 1471 1135 929 915 681 525 390 382 219 191 40 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8611 SgrAI Cr'CCGG_yG Cuts at: 1700 3196 1700 Size: 1496 7115 SmaI CCC'GGG Cuts at: 1872 5938 1872 Size: 4066 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2118 4544 5043 5499 5770 6715 Size: 197 926 175 2426 499 456 271 945 Cuts at: 6715 7616 7878 820 Size: 901 262 1553 Fragments arranged by size: 2426 1553 945 926 901 499 456 271 262 197 175 SnaBI TAC'GTA Cuts at: 493 6402 493 Size: 5909 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8611 SphI G_CATG'C Cuts at: 1132 2317 5969 1132 Size: 1185 3652 3774 Fragments arranged by size: 3774 3652 1185 SspI AAT'ATT Cuts at: 5 52 4040 5513 6535 5 Size: 47 3988 1473 1022 2081 Fragments arranged by size: 3988 2081 1473 1022 47 StuI AGG'CCT Cuts at: 1517 2127 1517 Size: 610 8001 StyI C'CwwG_G Cuts at: 513 1065 2004 2224 2290 2601 2692 2876 3253 Size: 552 939 220 66 311 91 184 377 Cuts at: 3253 5230 6050 513 Size: 1977 820 3074 Fragments arranged by size: 3074 1977 939 820 552 377 311 220 184 91 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3284 6638 6655 6808 6993 7438 325 Size: 1255 1704 3354 17 153 185 445 1498 Fragments arranged by size: 3354 1704 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2334 2636 5343 Size: 274 80 33 51 1443 357 302 2707 Cuts at: 5343 5535 6857 8569 96 Size: 192 1322 1712 138 Fragments arranged by size: 2707 1712 1443 1322 357 302 274 192 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2923 2955 3771 3803 4997 5029 5851 5883 8571 Size: 32 816 32 1194 32 822 32 2688 Cuts at: 8571 8603 2923 Size: 32 2931 Fragments arranged by size: 2931 2688 1194 822 816 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8611 XbaI T'CTAG_A Cuts at: 5947 5947 Size: 8611 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3317 3317 Size: 8611 XhoI C'TCGA_G Cuts at: 1943 5770 1943 Size: 3827 4784 XmaI C'CCGG_G Cuts at: 1870 5936 1870 Size: 4066 4545 XmnI GAAnn'nnTTC Cuts at: 5487 6740 5487 Size: 1253 7358 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6419 276 Size: 53 83 186 936 4885 2468 Fragments arranged by size: 4885 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI