(Circular) (Six-base) MAPSORT of: pFN21AB0170.seq Check: 7957 from: 1 to: 7918 pFN21AB0170 7918 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5072 5072 Size: 7918 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2345 5728 278 Size: 53 83 186 936 809 3383 2468 Fragments arranged by size: 3383 2468 936 809 186 83 53 AccI GT'mk_AC Cuts at: 1937 2193 4164 5261 1937 Size: 256 1971 1097 4594 Fragments arranged by size: 4594 1971 1097 256 Acc65I G'GTAC_C Cuts at: 5239 5239 Size: 7918 AclI AA'CG_TT Cuts at: 1563 6045 6418 1563 Size: 4482 373 3063 Fragments arranged by size: 4482 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2819 3099 3710 3863 4544 4910 5981 7365 Size: 1187 280 611 153 681 366 1071 1384 Cuts at: 7365 7633 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1187 1071 681 611 366 280 268 153 AflII C'TTAA_G Cuts at: 820 1017 2993 4949 820 Size: 197 1976 1956 3789 Fragments arranged by size: 3789 1976 1956 197 AflIII A'CryG_T Cuts at: 1203 3975 6817 7794 1203 Size: 2772 2842 977 1327 Fragments arranged by size: 2842 2772 1327 977 AgeI A'CCGG_T Cuts at: 6749 6749 Size: 7918 AhdI GACnn_n'nnGTC Cuts at: 6647 6647 Size: 7918 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7918 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2193 1184 Size: 1009 6909 AlwNI CAG_nnn'CTG Cuts at: 2416 5661 7233 2416 Size: 3245 1572 3101 Fragments arranged by size: 3245 3101 1572 ApaLI G'TGCA_C Cuts at: 3930 5975 7131 3930 Size: 2045 1156 4717 Fragments arranged by size: 4717 2045 1156 ApoI r'AATT_y Cuts at: 1493 2207 2247 2606 2647 3105 3332 3358 3481 Size: 714 40 359 41 458 227 26 123 Cuts at: 3481 3683 3716 3843 4140 4327 5075 5227 5453 Size: 202 33 127 297 187 748 152 226 Cuts at: 5453 5605 7714 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 748 714 458 359 297 227 226 202 187 152 152 127 123 41 40 33 26 AseI AT'TA_AT Cuts at: 160 1032 2741 3260 6472 160 Size: 872 1709 519 3212 1606 Fragments arranged by size: 3212 1709 1606 872 519 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7918 AvaI C'yCGr_G Cuts at: 1870 1943 5243 1870 Size: 73 3300 4545 Fragments arranged by size: 4545 3300 73 BamHI G'GATC_C Cuts at: 5248 5615 5248 Size: 367 7551 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5239 6694 618 Size: 325 217 627 3452 1455 1842 Fragments arranged by size: 3452 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2128 3676 5237 1391 Size: 737 1548 1561 4072 Fragments arranged by size: 4072 1561 1548 737 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3698 5094 928 Size: 971 1799 1396 3752 Fragments arranged by size: 3752 1799 1396 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3781 3815 6109 6143 1903 Size: 34 50 34 1760 34 2294 34 3678 Fragments arranged by size: 3678 2294 1760 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3337 5810 7020 3337 Size: 2473 1210 4235 Fragments arranged by size: 4235 2473 1210 BclI T'GATC_A Cuts at: 1548 3004 3310 1548 Size: 1456 306 6156 Fragments arranged by size: 6156 1456 306 BfrBI ATG'CAT Cuts at: 3529 3724 4828 4832 3529 Size: 195 1104 4 6615 Fragments arranged by size: 6615 1104 195 4 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6529 136 Size: 107 122 71 631 605 4857 1525 Fragments arranged by size: 4857 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7913 7913 Size: 7918 BlpI GC'TnA_GC Cuts at: 5335 5335 Size: 7918 Bme1580I G_kGCm'C Cuts at: 1790 3934 5979 7135 1790 Size: 2144 2045 1156 2573 Fragments arranged by size: 2573 2144 2045 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7918 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5649 6607 442 Size: 416 53 83 399 24 4232 958 1753 Fragments arranged by size: 4232 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7918 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4757 6578 1239 Size: 3518 1821 2579 Fragments arranged by size: 3518 2579 1821 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2127 2545 4965 6043 6908 7206 2127 Size: 418 2420 1078 865 298 2839 Fragments arranged by size: 2839 2420 1078 865 418 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2726 3722 6581 882 Size: 617 1227 996 2859 2219 Fragments arranged by size: 2859 2219 1227 996 617 BsaAI yAC'GTr Cuts at: 493 1204 5709 493 Size: 711 4505 2702 Fragments arranged by size: 4505 2702 711 BsaBI GATnn'nnATC Cuts at: 7912 7912 Size: 7918 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2342 5725 6107 6742 Size: 53 83 186 936 809 3383 382 635 Cuts at: 6742 275 Size: 1451 Fragments arranged by size: 3383 1451 936 809 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6351 6749 7023 7170 7581 6351 Size: 398 274 147 411 6688 Fragments arranged by size: 6688 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3309 3339 1178 Size: 30 165 30 1906 30 5757 Fragments arranged by size: 5757 1906 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7918 BseYI C'CCAG_C Cuts at: 2127 4381 4585 7121 7617 2127 Size: 2254 204 2536 496 2428 Fragments arranged by size: 2536 2428 2254 496 204 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2968 1163 Size: 573 1232 6113 Fragments arranged by size: 6113 1232 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5368 6129 7157 1540 Size: 275 186 3367 761 1028 2301 Fragments arranged by size: 3367 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3676 3934 5979 6064 7135 3676 Size: 258 2045 85 1071 4459 Fragments arranged by size: 4459 2045 1071 258 85 BsmI GAATG_Cn' Cuts at: 4493 4589 5429 5522 4493 Size: 96 840 93 6889 Fragments arranged by size: 6889 840 96 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2128 3676 3934 5237 5979 6064 7135 Size: 399 338 1548 258 1303 742 85 1071 Cuts at: 7135 1391 Size: 2174 Fragments arranged by size: 2174 1548 1303 1071 742 399 338 258 85 BspEI T'CCGG_A Cuts at: 7581 7581 Size: 7918 BspHI T'CATG_A Cuts at: 3336 5807 7537 3336 Size: 2471 1730 3717 Fragments arranged by size: 3717 2471 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5072 5273 844 Size: 4228 201 3489 Fragments arranged by size: 4228 3489 201 BsrBI CCG'CTC Cuts at: 1394 1823 5805 1394 Size: 429 3982 3507 Fragments arranged by size: 3982 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2608 2687 3531 6413 6587 65 Size: 1183 1360 79 844 2882 174 1396 Fragments arranged by size: 2882 1396 1360 1183 844 174 79 BsrFI r'CCGG_y Cuts at: 1700 1810 6562 6749 1700 Size: 110 4752 187 2869 Fragments arranged by size: 4752 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2668 3639 96 Size: 2572 971 4375 Fragments arranged by size: 4375 2572 971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7918 BssSI C'ACGA_G Cuts at: 5978 6990 5978 Size: 1012 6906 BstAPI GCAn_nnn'nTGC Cuts at: 794 2036 4595 794 Size: 1242 2559 4117 Fragments arranged by size: 4117 2559 1242 BstBI TT'CG_AA Cuts at: 2785 3714 6722 2785 Size: 929 3008 3981 Fragments arranged by size: 3981 3008 929 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7918 BstXI CCAn_nnnn'nTGG Cuts at: 4030 4208 4030 Size: 178 7740 BstYI r'GATC_y Cuts at: 1972 3571 4045 5248 5615 6000 6017 7469 7555 Size: 1599 474 1203 367 385 17 1452 86 Cuts at: 7555 7567 7913 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1599 1452 1203 474 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4165 4165 Size: 7918 BtgI C'CryG_G Cuts at: 513 1065 2113 513 Size: 552 1048 6318 Fragments arranged by size: 6318 1048 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7179 Fragments arranged by size: 7179 683 56 BtsI GCAGTG_nn' Cuts at: 5434 6228 6248 5434 Size: 794 20 7104 Fragments arranged by size: 7104 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7918 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3648 3683 529 Size: 35 3084 35 4764 Fragments arranged by size: 4764 3084 35 35 DraI TTT'AAA Cuts at: 2163 2586 2904 3103 4272 5222 2163 Size: 423 318 199 1169 950 4859 Fragments arranged by size: 4859 1169 950 423 318 199 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6925 809 Size: 507 5609 1802 Fragments arranged by size: 5609 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2441 4416 5365 Size: 54 1425 50 275 18 611 1975 949 Cuts at: 5365 6254 7883 8 Size: 889 1629 43 Fragments arranged by size: 1975 1629 1425 949 889 611 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5365 1537 Size: 275 3553 4090 Fragments arranged by size: 4090 3553 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3528 5106 5139 5848 1353 Size: 2175 1578 33 709 3423 Fragments arranged by size: 3423 2175 1578 709 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6505 6879 7025 387 Size: 1064 138 4916 374 146 1280 Fragments arranged by size: 4916 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3674 3674 Size: 7918 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2819 3099 3710 3863 4544 4757 4910 Size: 393 1187 280 611 153 681 213 153 Cuts at: 4910 5981 6578 7365 7633 1239 Size: 1071 597 787 268 1524 Fragments arranged by size: 1524 1187 1071 787 681 611 597 393 280 268 213 153 153 EcoNI CCTnn'n_nnAGG Cuts at: 5132 5132 Size: 7918 EcoO109I rG'GnC_Cy Cuts at: 3052 3052 Size: 7918 EcoRI G'AATT_C Cuts at: 2606 3332 3716 5227 5605 2606 Size: 726 384 1511 378 4919 Fragments arranged by size: 4919 1511 726 384 378 EcoRV GAT'ATC Cuts at: 2077 2077 Size: 7918 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4284 4975 4284 Size: 691 7227 FspI TGC'GCA Cuts at: 6424 6424 Size: 7918 HaeII r_GCGC'y Cuts at: 1678 7065 1678 Size: 5387 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3015 3047 4732 4764 6559 Size: 32 135 32 1443 32 1685 32 1795 Cuts at: 6559 6591 6633 6665 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1795 1685 1443 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2194 3197 3703 4429 4909 5262 Size: 988 281 256 1003 506 726 480 353 Cuts at: 5262 5508 669 Size: 246 3079 Fragments arranged by size: 3079 1003 988 726 506 480 353 281 256 246 HindIII A'AGCT_T Cuts at: 748 3440 748 Size: 2692 5226 HpaI GTT'AAC Cuts at: 3703 4909 5508 3703 Size: 1206 599 6113 Fragments arranged by size: 6113 1206 599 KpnI G_GTAC'C Cuts at: 5243 5243 Size: 7918 MfeI C'AATT_G Cuts at: 5517 5517 Size: 7918 MluI A'CGCG_T Cuts at: 7794 7794 Size: 7918 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2446 3796 4090 4326 7032 7216 1305 Size: 1141 1350 294 236 2706 184 2007 Fragments arranged by size: 2706 2007 1350 1141 294 236 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2443 4418 10 Size: 54 1425 343 611 1975 3510 Fragments arranged by size: 3510 1975 1425 611 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2175 3204 5876 6235 6394 518 Size: 648 1009 1029 2672 359 159 2042 Fragments arranged by size: 2672 2042 1029 1009 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 4385 4454 5334 6011 7159 7404 1606 Size: 444 2335 69 880 677 1148 245 2120 Fragments arranged by size: 2335 2120 1148 880 677 444 245 69 NcoI C'CATG_G Cuts at: 513 1065 2113 513 Size: 552 1048 6318 Fragments arranged by size: 6318 1048 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7918 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7918 NotI GC'GGCC_GC Cuts at: 5365 5365 Size: 7918 NsiI A_TGCA'T Cuts at: 3531 3726 4830 4834 3531 Size: 195 1104 4 6615 Fragments arranged by size: 6615 1104 195 4 NspI r_CATG'y Cuts at: 1132 1229 2599 2675 3979 4832 5276 6821 1132 Size: 97 1370 76 1304 853 444 1545 2229 Fragments arranged by size: 2229 1545 1370 1304 853 444 97 76 PasI CC'CwG_GG Cuts at: 1348 2716 1348 Size: 1368 6550 PciI A'CATG_T Cuts at: 3975 6817 3975 Size: 2842 5076 PflMI CCAn_nnn'nTGG Cuts at: 1108 2071 2439 3751 4641 1108 Size: 963 368 1312 890 4385 Fragments arranged by size: 4385 1312 963 890 368 PfoI T'CCnGG_A Cuts at: 3744 4894 3744 Size: 1150 6768 PmeI GTTT'AAAC Cuts at: 5222 5222 Size: 7918 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5987 6019 7500 7532 5987 Size: 32 1481 32 6373 Fragments arranged by size: 6373 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2189 1613 Size: 576 7342 PsiI TTA'TAA Cuts at: 2760 3566 5488 2760 Size: 806 1922 5190 Fragments arranged by size: 5190 1922 806 PstI C_TGCA'G Cuts at: 830 4195 4735 5270 830 Size: 3365 540 535 3478 Fragments arranged by size: 3478 3365 540 535 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7918 PvuII CAG'CTG Cuts at: 2050 4385 4454 2050 Size: 2335 69 5514 Fragments arranged by size: 5514 2335 69 SacI G_AGCT'C Cuts at: 3676 3676 Size: 7918 SalI G'TCGA_C Cuts at: 1936 2192 5260 1936 Size: 256 3068 4594 Fragments arranged by size: 4594 3068 256 SbfI CC_TGCA'GG Cuts at: 5270 5270 Size: 7918 ScaI AGT'ACT Cuts at: 2151 4411 6166 7878 2151 Size: 2260 1755 1712 2191 Fragments arranged by size: 2260 2191 1755 1712 SfcI C'TryA_G Cuts at: 826 1045 3145 3696 4191 4731 5266 6401 7082 Size: 219 2100 551 495 540 535 1135 681 Cuts at: 7082 7273 826 Size: 191 1471 Fragments arranged by size: 2100 1471 1135 681 551 540 535 495 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7918 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7918 SmaI CCC'GGG Cuts at: 1872 5245 1872 Size: 3373 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2142 2524 2993 4949 4980 6022 Size: 197 926 199 382 469 1956 31 1042 Cuts at: 6022 6923 7185 820 Size: 901 262 1553 Fragments arranged by size: 1956 1553 1042 926 901 469 382 262 199 197 31 SnaBI TAC'GTA Cuts at: 493 5709 493 Size: 5216 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7918 SphI G_CATG'C Cuts at: 1132 4832 5276 1132 Size: 3700 444 3774 Fragments arranged by size: 3774 3700 444 SspI AAT'ATT Cuts at: 5 52 2914 4186 4470 5842 5 Size: 47 2862 1272 284 1372 2081 Fragments arranged by size: 2862 2081 1372 1272 284 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7918 StyI C'CwwG_G Cuts at: 513 1065 2113 2288 2679 5357 513 Size: 552 1048 175 391 2678 3074 Fragments arranged by size: 3074 2678 1048 552 391 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4279 5945 5962 6115 6300 6745 325 Size: 1255 2699 1666 17 153 185 445 1498 Fragments arranged by size: 2699 1666 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2149 2668 3639 Size: 274 80 33 51 1443 172 519 971 Cuts at: 3639 3949 3972 4409 4880 6164 7876 96 Size: 310 23 437 471 1284 1712 138 Fragments arranged by size: 1712 1443 1284 971 519 471 437 310 274 172 138 80 51 33 23 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3309 3341 7878 7910 3309 Size: 32 4537 32 3317 Fragments arranged by size: 4537 3317 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7918 XbaI T'CTAG_A Cuts at: 5254 5254 Size: 7918 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7918 XmaI C'CCGG_G Cuts at: 1870 5243 1870 Size: 3373 4545 XmnI GAAnn'nnTTC Cuts at: 4547 6047 4547 Size: 1500 6418 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2343 5726 276 Size: 53 83 186 936 809 3383 2468 Fragments arranged by size: 3383 2468 936 809 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI