Restriction map for
pFN21AA2011
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII AccIII AclI AflII AgeI
AscI AseI AsnI AspI BbrPI
BbvCI BcgI BclI BfrI BglII
BsaAI BsaBI BseAI BsiEI BsiWI
BspEI BspHI BspLU11I BsrFI BssHII
Bst1107I BstEII BstPI BstZ17I Cfr10I
CpoI DraI EagI EclXI Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1405 GGTACC G^GTACC
AccI 2269 GTCTAC GT^[AC][GT]AC
AcsI 721 GAATTC [GA]^AATT[CT]
AcyI 1945 GGCGCC G[GA]^CG[CT]C
AflIII 183 ACGTGT A^C[GA][CT]GT
AhdI 1633 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2299 GTGCAC G^TGCAC
AocI 876 CCTGAGG CC^T[ACGT]AGG
ApaLI 2299 GTGCAC G^TGCAC
ApoI 721 GAATTC [GA]^AATT[CT]
Asp700I 2349 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1405 GGTACC G^GTACC
AspEI 1633 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2074 CCTAGG C^CTAGG
BamHI 2363 GGATCC G^GATCC
BbeI 1945 GGCGCC GGCGC^C
BciVI 657 GGATAC GTATCC(6/5)
BlnI 2074 CCTAGG C^CTAGG
BsaHI 1945 GGCGCC G[GA]^CG[CT]C
BsaI 1861 GAGACC GGTCTC(1/5)
BsgI 1346 CTGCAC GTGCAG(16/14)
BsmI 2442 GAATGC GAATGC(1/-1)
BspDI 901 ATCGAT AT^CGAT
BsrDI 1043 GCAATG GCAATG(2/0)
BstAPI 2015 GCATGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1216 TTCGAA TT^CGAA
Bsu36I 876 CCTGAGG CC^T[ACGT]AGG
ClaI 901 ATCGAT AT^CGAT
DraIII 224 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2527 GACTGGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1633 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 876 CCTGAGG CC^T[ACGT]AGG
EcoRI 721 GAATTC G^AATTC
Hin1I 1945 GGCGCC G[GA]^CG[CT]C
KasI 1945 GGCGCC G^GCGCC
KpnI 1405 GGTACC GGTAC^C
MseI 1852 TTAA T^TAA
NarI 1945 GGCGCC GG^CGCC
NcoI 2738 CCATGG C^CATGG
NspI 113 GCATGT [GA]CATG^[CT]
NspV 1216 TTCGAA TT^CGAA
PaeR7I 164 CTCGAG C^TCGAG
ScaI 845 AGTACT AGT^ACT
SexAI 661 ACCTGGT A^CC[AT]GGT
SfoI 1945 GGCGCC GGC^GCC
SfuI 1216 TTCGAA TT^CGAA
Tru9I 1852 TTAA T^TAA
Tsp509I 722 AATT ^AATT
XhoI 164 CTCGAG C^TCGAG
XmnI 2349 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 889 CGCG CG^CG
1879 CGCG
Aor51HI 2677 AGCGCT AGC^GCT
2868 AGCGCT
AviII 1671 TGCGCA TGC^GCA
2516 TGCGCA
BsaWI 437 ACCGGA [AT]^CCGG[AT]
1395 ACCGGA
BsmBI 2151 GAGACG CGTCTC(1/5)
2428 GAGACG
BsrBI 919 GAGCGG CCGCTC(-3/-3)
1609 GAGCGG
BssSI 2719 CACGAG CACGAG(-5/-1)
2474 CTCGTG
BstUI 889 CGCG CG^CG
1879 CGCG
BstXI 64 CCACGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
578 CCAATGGGCTGG
Eco47III 2677 AGCGCT AGC^GCT
2868 AGCGCT
FspI 1671 TGCGCA TGC^GCA
2516 TGCGCA
HgaI 2153 GACGC GACGC(5/10)
3021 GCGTC
MaeII 183 ACGT A^CGT
2504 ACGT
MslI 53 CACCACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1800 CACGGGCATG
MvnI 889 CGCG CG^CG
1879 CGCG
PpuMI 159 AGGTCCT [GA]G^G[AT]CC[CT]
624 AGGACCT
SacI 291 GAGCTC GAGCT^C
2434 GAGCTC
SapI 2349 GAAGAGC GCTCTTC(1/4)
2806 GAAGAGC
SfiI 930 GGCCGACTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1719 GGCCGATGTGGCC
XcmI 1466 CCAGAGCCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2228 CCAGCCCTTTCCTGG
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XhoII
XmaCI XmaI