(Circular) (Six-base) MAPSORT of: pFN21AA2011.seq Check: 5905 from: 1 to: 7777 pFN21AA2011 7777 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3004 3065 4702 3004 Size: 61 1637 6079 Fragments arranged by size: 6079 1637 61 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5587 278 Size: 53 83 186 936 4051 2468 Fragments arranged by size: 4051 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4275 5120 1937 Size: 2338 845 4594 Fragments arranged by size: 4594 2338 845 Acc65I G'GTAC_C Cuts at: 3410 5098 3410 Size: 1688 6089 AclI AA'CG_TT Cuts at: 1563 5904 6277 1563 Size: 4341 373 3063 Fragments arranged by size: 4341 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3138 3700 3745 3863 3948 4757 4830 4913 Size: 1506 562 45 118 85 809 73 83 Cuts at: 4913 5840 7224 7492 1632 Size: 927 1384 268 1917 Fragments arranged by size: 1917 1506 1384 927 809 562 268 118 85 83 73 45 AfeI AGC'GCT Cuts at: 4684 4875 4684 Size: 191 7586 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7580 AflIII A'CryG_T Cuts at: 1203 2188 6676 7653 1203 Size: 985 4488 977 1327 Fragments arranged by size: 4488 1327 985 977 AgeI A'CCGG_T Cuts at: 6608 6608 Size: 7777 AhdI GACnn_n'nnGTC Cuts at: 3643 6506 3643 Size: 2863 4914 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7777 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7777 AlwNI CAG_nnn'CTG Cuts at: 2984 3685 4316 4640 5520 7092 2984 Size: 701 631 324 880 1572 3669 Fragments arranged by size: 3669 1572 880 701 631 324 ApaI G_GGCC'C Cuts at: 2293 2600 2975 3127 4291 2293 Size: 307 375 152 1164 5779 Fragments arranged by size: 5779 1164 375 307 152 ApaLI G'TGCA_C Cuts at: 4304 5834 6990 4304 Size: 1530 1156 5091 Fragments arranged by size: 5091 1530 1156 ApoI r'AATT_y Cuts at: 1493 2726 5086 5312 5464 7573 1493 Size: 1233 2360 226 152 2109 1697 Fragments arranged by size: 2360 2109 1697 1233 226 152 AseI AT'TA_AT Cuts at: 160 1032 6331 160 Size: 872 5299 1606 Fragments arranged by size: 5299 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7777 AvaI C'yCGr_G Cuts at: 1870 1943 2169 2285 2679 4256 4335 4558 5016 Size: 73 226 116 394 1577 79 223 458 Cuts at: 5016 5102 1870 Size: 86 4545 Fragments arranged by size: 4545 1577 458 394 226 223 116 86 79 73 AvrII C'CTAG_G Cuts at: 4079 4079 Size: 7777 BamHI G'GATC_C Cuts at: 4368 5107 5474 4368 Size: 739 367 6671 Fragments arranged by size: 6671 739 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2015 2095 2254 2875 3254 Size: 325 217 627 228 80 159 621 379 Cuts at: 3254 3410 3738 3950 5098 6553 618 Size: 156 328 212 1148 1455 1842 Fragments arranged by size: 1842 1455 1148 627 621 379 328 325 228 217 212 159 156 80 BanII G_rGCy'C Cuts at: 1391 2108 2293 2300 2600 2810 2822 2975 3127 Size: 717 185 7 300 210 12 153 152 Cuts at: 3127 3185 3317 3501 4291 4443 5023 5096 1391 Size: 58 132 184 790 152 580 73 4072 Fragments arranged by size: 4072 790 717 580 300 210 185 184 153 152 152 132 73 58 12 7 BbeI G_GCGC'C Cuts at: 3954 3954 Size: 7777 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3793 4334 4504 4534 928 Size: 971 1894 541 170 30 4171 Fragments arranged by size: 4171 1894 971 541 170 30 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5968 6002 1903 Size: 34 4031 34 3678 Fragments arranged by size: 4031 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2656 5669 6879 2656 Size: 3013 1210 3554 Fragments arranged by size: 3554 3013 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7777 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2942 2979 3482 Size: 107 122 71 631 605 1270 37 503 Cuts at: 3482 3731 6388 136 Size: 249 2657 1525 Fragments arranged by size: 2657 1525 1270 631 605 503 249 122 107 71 37 BglII A'GATC_T Cuts at: 7772 7772 Size: 7777 BlpI GC'TnA_GC Cuts at: 2766 2922 5022 5194 2766 Size: 156 2100 172 5349 Fragments arranged by size: 5349 2100 172 156 Bme1580I G_kGCm'C Cuts at: 1790 2100 2257 2293 2600 2880 2975 3127 3165 Size: 310 157 36 307 280 95 152 38 Cuts at: 3165 4291 4308 5838 6994 1790 Size: 1126 17 1530 1156 2573 Fragments arranged by size: 2573 1530 1156 1126 310 307 280 157 152 95 38 36 17 BmgBI CAC'GTC Cuts at: 1318 2189 4510 1318 Size: 871 2321 4585 Fragments arranged by size: 4585 2321 871 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2192 2280 5508 Size: 416 53 83 399 24 775 88 3228 Cuts at: 5508 6466 442 Size: 958 1753 Fragments arranged by size: 3228 1753 958 775 416 399 88 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7777 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2956 4179 2956 Size: 1223 6554 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2346 3254 3302 3689 4070 4216 4586 4859 Size: 1107 908 48 387 381 146 370 273 Cuts at: 4859 4963 4990 6437 1239 Size: 104 27 1447 2579 Fragments arranged by size: 2579 1447 1107 908 387 381 370 273 146 104 48 27 Bpu10I CC'TnA_GC Cuts at: 2604 2758 3569 2604 Size: 154 811 6812 Fragments arranged by size: 6812 811 154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2392 2794 3134 3773 4076 4358 4427 5902 6767 Size: 402 340 639 303 282 69 1475 865 Cuts at: 6767 7065 2392 Size: 298 3104 Fragments arranged by size: 3104 1475 865 639 402 340 303 298 282 69 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3860 6440 882 Size: 617 2361 2580 2219 Fragments arranged by size: 2580 2361 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5568 493 Size: 711 4364 2702 Fragments arranged by size: 4364 2702 711 BsaBI GATnn'nnATC Cuts at: 7771 7771 Size: 7777 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3951 5584 5966 6601 Size: 53 83 186 936 2418 1633 382 635 Cuts at: 6601 275 Size: 1451 Fragments arranged by size: 2418 1633 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2442 3400 6210 6608 6882 7029 7440 2442 Size: 958 2810 398 274 147 411 2779 Fragments arranged by size: 2810 2779 958 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7552 Fragments arranged by size: 7552 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2394 2505 2563 2680 2945 3467 3470 3473 Size: 1208 111 58 117 265 522 3 3 Cuts at: 3473 4160 4163 4418 4721 4922 1186 Size: 687 3 255 303 201 4041 Fragments arranged by size: 4041 1208 687 522 303 265 255 201 117 111 58 3 3 3 BseYI C'CCAG_C Cuts at: 2110 2382 2419 2590 2753 2960 3032 3535 3776 Size: 272 37 171 163 207 72 503 241 Cuts at: 3776 3903 4633 4679 4776 4780 6980 7476 2110 Size: 127 730 46 97 4 2200 496 2411 Fragments arranged by size: 2411 2200 730 503 496 272 241 207 171 163 127 97 72 46 37 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3336 1163 Size: 573 1600 5604 Fragments arranged by size: 5604 1600 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5227 5988 7016 1540 Size: 275 186 3226 761 1028 2301 Fragments arranged by size: 3226 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2300 4308 4443 4767 4952 5838 5923 6994 2300 Size: 2008 135 324 185 886 85 1071 3083 Fragments arranged by size: 3083 2008 1071 886 324 185 135 85 BsmI GAATG_Cn' Cuts at: 4453 5288 5381 4453 Size: 835 93 6849 Fragments arranged by size: 6849 835 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4150 4427 4150 Size: 277 7500 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2108 2257 2293 2300 2600 2810 Size: 399 310 8 149 36 7 300 210 Cuts at: 2810 2822 2880 2975 3127 3165 3185 3317 3501 Size: 12 58 95 152 38 20 132 184 Cuts at: 3501 4291 4308 4443 4767 4952 5023 5096 5838 Size: 790 17 135 324 185 71 73 742 Cuts at: 5838 5923 6994 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 790 742 399 324 310 300 210 185 184 152 149 135 132 95 85 73 71 58 38 36 20 17 12 8 7 BspEI T'CCGG_A Cuts at: 7440 7440 Size: 7777 BspHI T'CATG_A Cuts at: 5666 7396 5666 Size: 1730 6047 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2147 2365 2641 3004 3065 4034 4401 4527 Size: 1303 218 276 363 61 969 367 126 Cuts at: 4527 4702 5132 844 Size: 175 430 3489 Fragments arranged by size: 3489 1303 969 430 367 363 276 218 175 126 61 BsrBI CCG'CTC Cuts at: 1394 1823 2926 3616 5664 1394 Size: 429 1103 690 2048 3507 Fragments arranged by size: 3507 2048 1103 690 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3055 6272 6446 65 Size: 1183 1807 3217 174 1396 Fragments arranged by size: 3217 1807 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6421 6608 1700 Size: 110 4611 187 2869 Fragments arranged by size: 4611 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2204 2524 4101 96 Size: 2108 320 1577 3772 Fragments arranged by size: 3772 2108 1577 320 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7777 BssSI C'ACGA_G Cuts at: 4479 4724 5837 6849 4479 Size: 245 1113 1012 5407 Fragments arranged by size: 5407 1113 1012 245 BstAPI GCAn_nnn'nTGC Cuts at: 794 4026 794 Size: 3232 4545 BstBI TT'CG_AA Cuts at: 3222 6581 3222 Size: 3359 4418 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7777 BstXI CCAn_nnnn'nTGG Cuts at: 2076 2590 2076 Size: 514 7263 BstYI r'GATC_y Cuts at: 1972 2386 3991 4368 5107 5474 5859 5876 7328 Size: 414 1605 377 739 367 385 17 1452 Cuts at: 7328 7414 7426 7772 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1605 1452 739 414 385 377 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2882 2882 Size: 7777 BtgI C'CryG_G Cuts at: 513 1065 2004 3002 3997 4363 4743 513 Size: 552 939 998 995 366 380 3547 Fragments arranged by size: 3547 998 995 939 552 380 366 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7038 Fragments arranged by size: 7038 683 56 BtsI GCAGTG_nn' Cuts at: 2982 3049 4136 4225 4231 4909 4926 5065 5293 Size: 67 1087 89 6 678 17 139 228 Cuts at: 5293 6087 6107 2982 Size: 794 20 4652 Fragments arranged by size: 4652 1087 794 678 228 139 89 67 20 17 6 ClaI AT'CG_AT Cuts at: 1554 2907 1554 Size: 1353 6424 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7742 DraI TTT'AAA Cuts at: 5081 5081 Size: 7777 DraIII CAC_nnn'GTG Cuts at: 2234 2234 Size: 7777 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4538 6784 809 Size: 507 3222 2246 1802 Fragments arranged by size: 3222 2246 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2613 2943 3128 Size: 54 1425 50 275 18 783 330 185 Cuts at: 3128 3687 4000 4176 4473 5045 5224 6113 7742 Size: 559 313 176 297 572 179 889 1629 Cuts at: 7742 8 Size: 43 Fragments arranged by size: 1629 1425 889 783 572 559 330 313 297 275 185 179 176 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5224 1537 Size: 275 3412 4090 Fragments arranged by size: 4090 3412 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2266 2627 4349 4553 4806 5707 1353 Size: 913 361 1722 204 253 901 3423 Fragments arranged by size: 3423 1722 913 901 361 253 204 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5083 6364 6738 6884 387 Size: 1064 138 3494 1281 374 146 1280 Fragments arranged by size: 3494 1281 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2298 4441 2298 Size: 2143 5634 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2346 3138 3254 3302 3689 3700 3745 Size: 393 714 792 116 48 387 11 45 Cuts at: 3745 3863 3948 4070 4216 4586 4757 4830 4859 Size: 118 85 122 146 370 171 73 29 Cuts at: 4859 4913 4963 4990 5840 6437 7224 7492 1239 Size: 54 50 27 850 597 787 268 1524 Fragments arranged by size: 1524 850 792 787 714 597 393 387 370 268 171 146 122 118 116 85 73 54 50 48 45 29 27 11 EcoNI CCTnn'n_nnAGG Cuts at: 2361 2654 4397 4550 4700 5046 2361 Size: 293 1743 153 150 346 5092 Fragments arranged by size: 5092 1743 346 293 153 150 EcoO109I rG'GnC_Cy Cuts at: 2165 2289 2596 2597 2630 2782 2971 3123 4054 Size: 124 307 1 33 152 189 152 931 Cuts at: 4054 4287 4288 4423 4503 4555 2165 Size: 233 1 135 80 52 5387 Fragments arranged by size: 5387 931 307 233 189 152 152 135 124 80 52 33 1 1 EcoRI G'AATT_C Cuts at: 2726 5086 5464 2726 Size: 2360 378 5039 Fragments arranged by size: 5039 2360 378 FspI TGC'GCA Cuts at: 3678 4523 6283 3678 Size: 845 1760 5172 Fragments arranged by size: 5172 1760 845 HaeII r_GCGC'y Cuts at: 1678 2611 3685 3954 4686 4877 6924 1678 Size: 933 1074 269 732 191 2047 2531 Fragments arranged by size: 2531 2047 1074 933 732 269 191 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2371 2403 2654 2686 3578 Size: 32 135 32 799 32 251 32 892 Cuts at: 3578 3610 3629 3661 6418 6450 6492 6524 1373 Size: 32 19 32 2757 32 42 32 2626 Fragments arranged by size: 2757 2626 892 799 251 135 42 32 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 5121 5367 669 Size: 988 281 3183 246 3079 Fragments arranged by size: 3183 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7777 HpaI GTT'AAC Cuts at: 5367 5367 Size: 7777 KasI G'GCGC_C Cuts at: 3950 3950 Size: 7777 KpnI G_GTAC'C Cuts at: 3414 5102 3414 Size: 1688 6089 MfeI C'AATT_G Cuts at: 5376 5376 Size: 7777 MluI A'CGCG_T Cuts at: 7653 7653 Size: 7777 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2357 2463 2533 3609 6891 7075 1305 Size: 1052 106 70 1076 3282 184 2007 Fragments arranged by size: 3282 2007 1076 1052 184 106 70 MscI TGG'CCA Cuts at: 10 64 1489 1832 2615 3689 4475 10 Size: 54 1425 343 783 1074 786 3312 Fragments arranged by size: 3312 1425 1074 786 783 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2062 3809 5735 6094 6253 518 Size: 648 896 1747 1926 359 159 2042 Fragments arranged by size: 2042 1926 1747 896 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2076 3577 4212 4529 4637 4780 4819 5193 Size: 470 1501 635 317 108 143 39 374 Cuts at: 5193 5870 7018 7263 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1501 1148 677 635 470 374 317 245 143 108 39 NarI GG'CG_CC Cuts at: 3951 3951 Size: 7777 NcoI C'CATG_G Cuts at: 513 1065 2004 4743 513 Size: 552 939 2739 3547 Fragments arranged by size: 3547 2739 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7777 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7777 NotI GC'GGCC_GC Cuts at: 5224 5224 Size: 7777 NspI r_CATG'y Cuts at: 1132 1229 2122 5135 6680 1132 Size: 97 893 3013 1545 2229 Fragments arranged by size: 3013 2229 1545 893 97 PasI CC'CwG_GG Cuts at: 1348 2011 2250 3488 3625 1348 Size: 663 239 1238 137 5500 Fragments arranged by size: 5500 1238 663 239 137 PciI A'CATG_T Cuts at: 6676 6676 Size: 7777 PflMI CCAn_nnn'nTGG Cuts at: 1108 2348 2426 3020 4471 1108 Size: 1240 78 594 1451 4414 Fragments arranged by size: 4414 1451 1240 594 78 PfoI T'CCnGG_A Cuts at: 3231 4242 4580 3231 Size: 1011 338 6428 Fragments arranged by size: 6428 1011 338 PmeI GTTT'AAAC Cuts at: 5081 5081 Size: 7777 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5846 5878 7359 7391 5846 Size: 32 1481 32 6232 Fragments arranged by size: 6232 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2165 2630 2165 Size: 465 7312 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7777 PsiI TTA'TAA Cuts at: 5347 5347 Size: 7777 PspOMI G'GGCC_C Cuts at: 2289 2596 2971 3123 4287 2289 Size: 307 375 152 1164 5779 Fragments arranged by size: 5779 1164 375 307 152 PspXI vC'TCGA_Gb Cuts at: 2169 2169 Size: 7777 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3776 3808 3776 Size: 32 7745 PstI C_TGCA'G Cuts at: 830 4131 4325 4398 4995 5129 830 Size: 3301 194 73 597 134 3478 Fragments arranged by size: 3478 3301 597 194 134 73 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7777 PvuII CAG'CTG Cuts at: 3577 4529 4637 4780 3577 Size: 952 108 143 6574 Fragments arranged by size: 6574 952 143 108 SacI G_AGCT'C Cuts at: 2300 4443 2300 Size: 2143 5634 SalI G'TCGA_C Cuts at: 1936 5119 1936 Size: 3183 4594 SapI GCTCTTCn'nnn_ Cuts at: 4349 4806 4349 Size: 457 7320 SbfI CC_TGCA'GG Cuts at: 5129 5129 Size: 7777 ScaI AGT'ACT Cuts at: 2852 6025 7737 2852 Size: 3173 1712 2892 Fragments arranged by size: 3173 2892 1712 SexAI A'CCwGG_T Cuts at: 2666 2666 Size: 7777 SfcI C'TryA_G Cuts at: 826 1045 4127 4321 4394 4492 4858 4991 5125 Size: 219 3082 194 73 98 366 133 134 Cuts at: 5125 6260 6941 7132 826 Size: 1135 681 191 1471 Fragments arranged by size: 3082 1471 1135 681 366 219 194 191 134 133 98 73 SfiI GGCCn_nnn'nGGCC Cuts at: 2942 3731 2942 Size: 789 6988 SfoI GGC'GCC Cuts at: 3952 3952 Size: 7777 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7777 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7777 SmaI CCC'GGG Cuts at: 1872 2287 2681 5018 5104 1872 Size: 415 394 2337 86 4545 Fragments arranged by size: 4545 2337 415 394 86 SmlI C'TyrA_G Cuts at: 820 1017 1943 2169 2371 2809 3149 3788 4091 Size: 197 926 226 202 438 340 639 303 Cuts at: 4091 4373 4442 5881 6782 7044 820 Size: 282 69 1439 901 262 1553 Fragments arranged by size: 1553 1439 926 901 639 438 340 303 282 262 226 202 197 69 SnaBI TAC'GTA Cuts at: 493 5568 493 Size: 5075 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7777 SphI G_CATG'C Cuts at: 1132 5135 1132 Size: 4003 3774 SspI AAT'ATT Cuts at: 5 52 5701 5 Size: 47 5649 2081 Fragments arranged by size: 5649 2081 47 StuI AGG'CCT Cuts at: 1517 3113 3287 4097 4832 1517 Size: 1596 174 810 735 4462 Fragments arranged by size: 4462 1596 810 735 174 StyI C'CwwG_G Cuts at: 513 1065 2004 2425 2742 3288 3434 3529 3550 Size: 552 939 421 317 546 146 95 21 Cuts at: 3550 4079 4191 4743 5216 513 Size: 529 112 552 473 3074 Fragments arranged by size: 3074 939 552 552 546 529 473 421 317 146 112 95 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2281 2381 3851 5804 5821 5974 6159 Size: 1255 701 100 1470 1953 17 153 185 Cuts at: 6159 6604 325 Size: 445 1498 Fragments arranged by size: 1953 1498 1470 1255 701 445 185 153 100 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2204 2524 2850 Size: 274 80 33 51 1443 227 320 326 Cuts at: 2850 4101 4407 6023 7735 96 Size: 1251 306 1616 1712 138 Fragments arranged by size: 1712 1616 1443 1251 326 320 306 274 227 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7737 7769 7737 Size: 32 7745 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7777 XbaI T'CTAG_A Cuts at: 5113 5113 Size: 7777 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3478 4240 3478 Size: 762 7015 XhoI C'TCGA_G Cuts at: 1943 2169 1943 Size: 226 7551 XmaI C'CCGG_G Cuts at: 1870 2285 2679 5016 5102 1870 Size: 415 394 2337 86 4545 Fragments arranged by size: 4545 2337 415 394 86 XmnI GAAnn'nnTTC Cuts at: 4358 5906 4358 Size: 1548 6229 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5585 276 Size: 53 83 186 936 4051 2468 Fragments arranged by size: 4051 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbvCI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI RsrII SacII SanDI SrfI SwaI