Restriction map for
pFN21AA2011
(Linear) (Six-base) MAPSORT of: KIAA2011.seq Check: 5072 from: 1 to: 3072
KIAA2011.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 999 1060 2697 3072
Size: 999 61 1637 375
Fragments arranged by size:
1637 999 375 61
AccI GT'mk_AC
Cuts at: 0 2270 3072
Size: 2270 802
Acc65I G'GTAC_C
Cuts at: 0 1405 3072
Size: 1405 1667
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1133 1695 1740 1858 1943 2752 2825 2908
Size: 1133 562 45 118 85 809 73 83
Cuts at: 2908 3072
Size: 164
Fragments arranged by size:
1133 809 562 164 118 85 83 73
45
AfeI AGC'GCT
Cuts at: 0 2679 2870 3072
Size: 2679 191 202
Fragments arranged by size:
2679 202 191
AflIII A'CryG_T
Cuts at: 0 183 3072
Size: 183 2889
AhdI GACnn_n'nnGTC
Cuts at: 0 1638 3072
Size: 1638 1434
AlwNI CAG_nnn'CTG
Cuts at: 0 979 1680 2311 2635 3072
Size: 979 701 631 324 437
Fragments arranged by size:
979 701 631 437 324
ApaI G_GGCC'C
Cuts at: 0 288 595 970 1122 2286 3072
Size: 288 307 375 152 1164 786
Fragments arranged by size:
1164 786 375 307 288 152
ApaLI G'TGCA_C
Cuts at: 0 2299 3072
Size: 2299 773
ApoI r'AATT_y
Cuts at: 0 721 3072
Size: 721 2351
AvaI C'yCGr_G
Cuts at: 0 164 280 674 2251 2330 2553 3011 3072
Size: 164 116 394 1577 79 223 458 61
Fragments arranged by size:
1577 458 394 223 164 116 79 61
AvrII C'CTAG_G
Cuts at: 0 2074 3072
Size: 2074 998
BamHI G'GATC_C
Cuts at: 0 2363 3072
Size: 2363 709
BanI G'GyrC_C
Cuts at: 0 10 90 249 870 1249 1405 1733 1945
Size: 10 80 159 621 379 156 328 212
Cuts at: 1945 3072
Size: 1127
Fragments arranged by size:
1127 621 379 328 212 159 156 80
10
BanII G_rGCy'C
Cuts at: 0 103 288 295 595 805 817 970 1122
Size: 103 185 7 300 210 12 153 152
Cuts at: 1122 1180 1312 1496 2286 2438 3018 3072
Size: 58 132 184 790 152 580 54
Fragments arranged by size:
790 580 300 210 185 184 153 152
152 132 103 58 54 12 7
BbeI G_GCGC'C
Cuts at: 0 1949 3072
Size: 1949 1123
BbsI GAAGACnn'nnnn_
Cuts at: 0 1788 2329 2499 2529 3072
Size: 1788 541 170 30 543
Fragments arranged by size:
1788 543 541 170 30
BciVI GTATCCnnnnn_n'
Cuts at: 0 651 3072
Size: 651 2421
BglI GCCn_nnn'nGGC
Cuts at: 0 937 974 1477 1726 3072
Size: 937 37 503 249 1346
Fragments arranged by size:
1346 937 503 249 37
BlpI GC'TnA_GC
Cuts at: 0 761 917 3017 3072
Size: 761 156 2100 55
Fragments arranged by size:
2100 761 156 55
Bme1580I G_kGCm'C
Cuts at: 0 95 252 288 595 875 970 1122 1160
Size: 95 157 36 307 280 95 152 38
Cuts at: 1160 2286 2303 3072
Size: 1126 17 769
Fragments arranged by size:
1126 769 307 280 157 152 95 95
38 36 17
BmgBI CAC'GTC
Cuts at: 0 184 2505 3072
Size: 184 2321 567
Fragments arranged by size:
2321 567 184
BmrI ACTGGGnnnn_n'
Cuts at: 0 187 275 3072
Size: 187 88 2797
Fragments arranged by size:
2797 187 88
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 951 2174 3072
Size: 951 1223 898
Fragments arranged by size:
1223 951 898
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 1249 1297 1684 2065 2211 2581 2854
Size: 341 908 48 387 381 146 370 273
Cuts at: 2854 2958 2985 3072
Size: 104 27 87
Fragments arranged by size:
908 387 381 370 341 273 146 104
87 48 27
Bpu10I CC'TnA_GC
Cuts at: 0 599 753 1564 3072
Size: 599 154 811 1508
Fragments arranged by size:
1508 811 599 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 789 1129 1768 2071 2353 2422 3072
Size: 387 402 340 639 303 282 69 650
Fragments arranged by size:
650 639 402 387 340 303 282 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 1855 3072
Size: 1855 1217
BsaHI Gr'CG_yC
Cuts at: 0 1946 3072
Size: 1946 1126
BsaWI w'CCGG_w
Cuts at: 0 437 1395 3072
Size: 437 958 1677
Fragments arranged by size:
1677 958 437
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 389 500 558 675 940 1462 1465 1468
Size: 389 111 58 117 265 522 3 3
Cuts at: 1468 2155 2158 2413 2716 2917 3072
Size: 687 3 255 303 201 155
Fragments arranged by size:
687 522 389 303 265 255 201 155
117 111 58 3 3 3
BseYI C'CCAG_C
Cuts at: 0 105 377 414 585 748 955 1027 1530
Size: 105 272 37 171 163 207 72 503
Cuts at: 1530 1771 1898 2628 2674 2771 2775 3072
Size: 241 127 730 46 97 4 297
Fragments arranged by size:
730 503 297 272 241 207 171 163
127 105 97 72 46 37 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1331 3072
Size: 1331 1741
BsiHKAI G_wGCw'C
Cuts at: 0 295 2303 2438 2762 2947 3072
Size: 295 2008 135 324 185 125
Fragments arranged by size:
2008 324 295 185 135 125
BsmI GAATG_Cn'
Cuts at: 0 2448 3072
Size: 2448 624
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2145 2422 3072
Size: 2145 277 650
Fragments arranged by size:
2145 650 277
Bsp1286I G_dGCh'C
Cuts at: 0 95 103 252 288 295 595 805 817
Size: 95 8 149 36 7 300 210 12
Cuts at: 817 875 970 1122 1160 1180 1312 1496 2286
Size: 58 95 152 38 20 132 184 790
Cuts at: 2286 2303 2438 2762 2947 3018 3072
Size: 17 135 324 185 71 54
Fragments arranged by size:
790 324 300 210 185 184 152 149
135 132 95 95 71 58 54 38
36 20 17 12 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 142 360 636 999 1060 2029 2396 2522
Size: 142 218 276 363 61 969 367 126
Cuts at: 2522 2697 3072
Size: 175 375
Fragments arranged by size:
969 375 367 363 276 218 175 142
126 61
BsrBI CCG'CTC
Cuts at: 0 921 1611 3072
Size: 921 690 1461
Fragments arranged by size:
1461 921 690
BsrDI GCAATG_nn'
Cuts at: 0 1050 3072
Size: 1050 2022
BsrGI T'GTAC_A
Cuts at: 0 199 519 2096 3072
Size: 199 320 1577 976
Fragments arranged by size:
1577 976 320 199
BssSI C'ACGA_G
Cuts at: 0 2474 2719 3072
Size: 2474 245 353
Fragments arranged by size:
2474 353 245
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2021 3072
Size: 2021 1051
BstBI TT'CG_AA
Cuts at: 0 1217 3072
Size: 1217 1855
BstXI CCAn_nnnn'nTGG
Cuts at: 0 71 585 3072
Size: 71 514 2487
Fragments arranged by size:
2487 514 71
BstYI r'GATC_y
Cuts at: 0 381 1986 2363 3072
Size: 381 1605 377 709
Fragments arranged by size:
1605 709 381 377
Bsu36I CC'TnA_GG
Cuts at: 0 877 3072
Size: 877 2195
BtgI C'CryG_G
Cuts at: 0 997 1992 2358 2738 3072
Size: 997 995 366 380 334
Fragments arranged by size:
997 995 380 366 334
BtsI GCAGTG_nn'
Cuts at: 0 977 1044 2131 2220 2226 2904 2921 3060
Size: 977 67 1087 89 6 678 17 139
Cuts at: 3060 3072
Size: 12
Fragments arranged by size:
1087 977 678 139 89 67 17 12
6
ClaI AT'CG_AT
Cuts at: 0 902 3072
Size: 902 2170
DraIII CAC_nnn'GTG
Cuts at: 0 229 3072
Size: 229 2843
DrdI GACnn_nn'nnGTC
Cuts at: 0 2533 3072
Size: 2533 539
EaeI y'GGCC_r
Cuts at: 0 608 938 1123 1682 1995 2171 2468 3040
Size: 608 330 185 559 313 176 297 572
Cuts at: 3040 3072
Size: 32
Fragments arranged by size:
608 572 559 330 313 297 185 176
32
EarI CTCTTCn'nnn_
Cuts at: 0 261 622 2344 2548 2801 3072
Size: 261 361 1722 204 253 271
Fragments arranged by size:
1722 361 271 261 253 204
EcoICRI GAG'CTC
Cuts at: 0 293 2436 3072
Size: 293 2143 636
Fragments arranged by size:
2143 636 293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 1133 1249 1297 1684 1695 1740 1858
Size: 341 792 116 48 387 11 45 118
Cuts at: 1858 1943 2065 2211 2581 2752 2825 2854 2908
Size: 85 122 146 370 171 73 29 54
Cuts at: 2908 2958 2985 3072
Size: 50 27 87
Fragments arranged by size:
792 387 370 341 171 146 122 118
116 87 85 73 54 50 48 45
29 27 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 356 649 2392 2545 2695 3041 3072
Size: 356 293 1743 153 150 346 31
Fragments arranged by size:
1743 356 346 293 153 150 31
EcoO109I rG'GnC_Cy
Cuts at: 0 160 284 591 592 625 777 966 1118
Size: 160 124 307 1 33 152 189 152
Cuts at: 1118 2049 2282 2283 2418 2498 2550 3072
Size: 931 233 1 135 80 52 522
Fragments arranged by size:
931 522 307 233 189 160 152 152
135 124 80 52 33 1 1
EcoRI G'AATT_C
Cuts at: 0 721 3072
Size: 721 2351
FspI TGC'GCA
Cuts at: 0 1673 2518 3072
Size: 1673 845 554
Fragments arranged by size:
1673 845 554
HaeII r_GCGC'y
Cuts at: 0 606 1680 1949 2681 2872 3072
Size: 606 1074 269 732 191 200
Fragments arranged by size:
1074 732 606 269 200 191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 366 398 649 681 1573 1605 1624 1656
Size: 366 32 251 32 892 32 19 32
Cuts at: 1656 3072
Size: 1416
Fragments arranged by size:
1416 892 366 251 32 32 32 32
19
KasI G'GCGC_C
Cuts at: 0 1945 3072
Size: 1945 1127
KpnI G_GTAC'C
Cuts at: 0 1409 3072
Size: 1409 1663
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 458 528 1604 3072
Size: 352 106 70 1076 1468
Fragments arranged by size:
1468 1076 352 106 70
MscI TGG'CCA
Cuts at: 0 610 1684 2470 3072
Size: 610 1074 786 602
Fragments arranged by size:
1074 786 610 602
MslI CAynn'nnrTG
Cuts at: 0 57 1804 3072
Size: 57 1747 1268
Fragments arranged by size:
1747 1268 57
MspA1I CmG'CkG
Cuts at: 0 71 1572 2207 2524 2632 2775 2814 3072
Size: 71 1501 635 317 108 143 39 258
Fragments arranged by size:
1501 635 317 258 143 108 71 39
NarI GG'CG_CC
Cuts at: 0 1946 3072
Size: 1946 1126
NcoI C'CATG_G
Cuts at: 0 2738 3072
Size: 2738 334
NspI r_CATG'y
Cuts at: 0 117 3072
Size: 117 2955
PasI CC'CwG_GG
Cuts at: 0 6 245 1483 1620 3072
Size: 6 239 1238 137 1452
Fragments arranged by size:
1452 1238 239 137 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 343 421 1015 2466 3072
Size: 343 78 594 1451 606
Fragments arranged by size:
1451 606 594 343 78
PfoI T'CCnGG_A
Cuts at: 0 1226 2237 2575 3072
Size: 1226 1011 338 497
Fragments arranged by size:
1226 1011 497 338
PpuMI rG'GwC_Cy
Cuts at: 0 160 625 3072
Size: 160 465 2447
Fragments arranged by size:
2447 465 160
PspOMI G'GGCC_C
Cuts at: 0 284 591 966 1118 2282 3072
Size: 284 307 375 152 1164 790
Fragments arranged by size:
1164 790 375 307 284 152
PspXI vC'TCGA_Gb
Cuts at: 0 164 3072
Size: 164 2908
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1771 1803 3072
Size: 1771 32 1269
Fragments arranged by size:
1771 1269 32
PstI C_TGCA'G
Cuts at: 0 2126 2320 2393 2990 3072
Size: 2126 194 73 597 82
Fragments arranged by size:
2126 597 194 82 73
PvuII CAG'CTG
Cuts at: 0 1572 2524 2632 2775 3072
Size: 1572 952 108 143 297
Fragments arranged by size:
1572 952 297 143 108
SacI G_AGCT'C
Cuts at: 0 295 2438 3072
Size: 295 2143 634
Fragments arranged by size:
2143 634 295
SapI GCTCTTCn'nnn_
Cuts at: 0 2344 2801 3072
Size: 2344 457 271
Fragments arranged by size:
2344 457 271
ScaI AGT'ACT
Cuts at: 0 847 3072
Size: 847 2225
SexAI A'CCwGG_T
Cuts at: 0 661 3072
Size: 661 2411
SfcI C'TryA_G
Cuts at: 0 2122 2316 2389 2487 2853 2986 3072
Size: 2122 194 73 98 366 133 86
Fragments arranged by size:
2122 366 194 133 98 86 73
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 937 1726 3072
Size: 937 789 1346
Fragments arranged by size:
1346 937 789
SfoI GGC'GCC
Cuts at: 0 1947 3072
Size: 1947 1125
SmaI CCC'GGG
Cuts at: 0 282 676 3013 3072
Size: 282 394 2337 59
Fragments arranged by size:
2337 394 282 59
SmlI C'TyrA_G
Cuts at: 0 164 366 804 1144 1783 2086 2368 2437
Size: 164 202 438 340 639 303 282 69
Cuts at: 2437 3072
Size: 635
Fragments arranged by size:
639 635 438 340 303 282 202 164
69
StuI AGG'CCT
Cuts at: 0 1108 1282 2092 2827 3072
Size: 1108 174 810 735 245
Fragments arranged by size:
1108 810 735 245 174
StyI C'CwwG_G
Cuts at: 0 420 737 1283 1429 1524 1545 2074 2186
Size: 420 317 546 146 95 21 529 112
Cuts at: 2186 2738 3072
Size: 552 334
Fragments arranged by size:
552 546 529 420 334 317 146 112
95 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 276 376 1846 3072
Size: 276 100 1470 1226
Fragments arranged by size:
1470 1226 276 100
TatI w'GTAC_w
Cuts at: 0 199 519 845 2096 2402 3072
Size: 199 320 326 1251 306 670
Fragments arranged by size:
1251 670 326 320 306 199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1473 2235 3072
Size: 1473 762 837
Fragments arranged by size:
1473 837 762
XhoI C'TCGA_G
Cuts at: 0 164 3072
Size: 164 2908
XmaI C'CCGG_G
Cuts at: 0 280 674 3011 3072
Size: 280 394 2337 61
Fragments arranged by size:
2337 394 280 61
XmnI GAAnn'nnTTC
Cuts at: 0 2353 3072
Size: 2353 719
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AlwNI
ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtsI ClaI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I KasI
KpnI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PflMI PfoI PpuMI PspOMI PspXI PsrI PstI
PvuII SacI SapI ScaI SexAI SfcI SfiI SfoI
SmaI SmlI StuI StyI TaqII TatI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AleI AlfI AloI AscI
AseI AsiSI BaeI BbvCI BcgI BclI BfrBI BglII
BmtI BsaAI BsaBI BsaXI BsiEI BsiWI BspEI BspHI
BsrFI BssHII BstEII BstZ17I BtgZI CspCI DraI EagI
EciI EcoRV FalI FseI FspAI HincII HindIII HpaI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PvuI RsrII SacII SalI SanDI SbfI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI ZraI