(Circular) (Six-base) MAPSORT of: pFN21AA1839.seq Check: 8580 from: 1 to: 6256 pFN21AA1839 6256 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2112 2112 Size: 6256 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3599 1937 Size: 1662 4594 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2165 2325 2786 2907 3084 3144 4319 5703 Size: 533 160 461 121 177 60 1175 1384 Cuts at: 5703 5971 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1175 533 461 268 177 160 121 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 3292 5155 6132 1203 Size: 2089 1863 977 1327 Fragments arranged by size: 2089 1863 1327 977 AgeI A'CCGG_T Cuts at: 5087 5087 Size: 6256 AhdI GACnn_n'nnGTC Cuts at: 4985 4985 Size: 6256 AleI CACnn'nnGTG Cuts at: 1166 2881 1166 Size: 1715 4541 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2043 1184 Size: 859 5397 AlwNI CAG_nnn'CTG Cuts at: 2348 3999 5571 2348 Size: 1651 1572 3033 Fragments arranged by size: 3033 1651 1572 ApaLI G'TGCA_C Cuts at: 4313 5469 4313 Size: 1156 5100 ApoI r'AATT_y Cuts at: 1493 2297 2464 2584 2969 3198 3267 3280 3565 Size: 804 167 120 385 229 69 13 285 Cuts at: 3565 3791 3943 6052 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 804 385 285 229 226 167 152 120 69 13 AseI AT'TA_AT Cuts at: 160 1032 2783 4810 160 Size: 872 1751 2027 1606 Fragments arranged by size: 2027 1751 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2073 3581 1870 Size: 73 72 58 1508 4545 Fragments arranged by size: 4545 1508 73 72 58 BamHI G'GATC_C Cuts at: 3586 3953 3586 Size: 367 5889 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2100 3577 5032 618 Size: 325 217 627 313 1477 1455 1842 Fragments arranged by size: 1842 1477 1455 627 325 313 217 BanII G_rGCy'C Cuts at: 1391 2054 2161 3575 1391 Size: 663 107 1414 4072 Fragments arranged by size: 4072 1414 663 107 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2640 3147 928 Size: 971 741 507 4037 Fragments arranged by size: 4037 971 741 507 BbvCI CC'TCA_GC Cuts at: 2124 2676 2124 Size: 552 5704 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4447 4481 1903 Size: 34 2510 34 3678 Fragments arranged by size: 3678 2510 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4148 5358 4148 Size: 1210 5046 BclI T'GATC_A Cuts at: 1548 1548 Size: 6256 BfrBI ATG'CAT Cuts at: 3074 3074 Size: 6256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4867 136 Size: 107 122 71 631 605 3195 1525 Fragments arranged by size: 3195 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6251 6251 Size: 6256 BlpI GC'TnA_GC Cuts at: 2112 3673 2112 Size: 1561 4695 Bme1580I G_kGCm'C Cuts at: 1790 4317 5473 1790 Size: 2527 1156 2573 Fragments arranged by size: 2573 2527 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3987 4945 442 Size: 416 53 83 399 24 2570 958 1753 Fragments arranged by size: 2570 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4916 1239 Size: 3677 2579 Bpu10I CC'TnA_GC Cuts at: 2124 2676 2124 Size: 552 5704 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2459 4381 5246 5544 2459 Size: 1922 865 298 3171 Fragments arranged by size: 3171 1922 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4919 882 Size: 617 3420 2219 Fragments arranged by size: 3420 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4047 493 Size: 711 2843 2702 Fragments arranged by size: 2843 2702 711 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4063 4445 5080 275 Size: 53 83 186 936 2530 382 635 1451 Fragments arranged by size: 2530 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4689 5087 5361 5508 5919 4689 Size: 398 274 147 411 5026 Fragments arranged by size: 5026 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6031 Fragments arranged by size: 6031 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2044 2047 2141 2664 3143 1186 Size: 858 3 94 523 479 4299 Fragments arranged by size: 4299 858 523 479 94 3 BseYI C'CCAG_C Cuts at: 5459 5955 5459 Size: 496 5760 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5683 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2083 3706 4467 5495 1540 Size: 275 186 82 1623 761 1028 2301 Fragments arranged by size: 2301 1623 1028 761 275 186 82 BsiHKAI G_wGCw'C Cuts at: 2054 4317 4402 5473 2054 Size: 2263 85 1071 2837 Fragments arranged by size: 2837 2263 1071 85 BsmI GAATG_Cn' Cuts at: 3767 3860 3767 Size: 93 6163 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2161 3575 4317 4402 5473 1391 Size: 399 264 107 1414 742 85 1071 2174 Fragments arranged by size: 2174 1414 1071 742 399 264 107 85 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 3046 4145 5875 3046 Size: 1099 1730 3427 Fragments arranged by size: 3427 1730 1099 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2112 3064 3611 844 Size: 1268 952 547 3489 Fragments arranged by size: 3489 1268 952 547 BsrBI CCG'CTC Cuts at: 1394 1823 4143 1394 Size: 429 2320 3507 Fragments arranged by size: 3507 2320 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2645 4751 4925 65 Size: 1183 1397 2106 174 1396 Fragments arranged by size: 2106 1397 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2107 4900 5087 1700 Size: 110 297 2793 187 2869 Fragments arranged by size: 2869 2793 297 187 110 BsrGI T'GTAC_A Cuts at: 96 2554 96 Size: 2458 3798 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6256 BssSI C'ACGA_G Cuts at: 2047 4316 5328 2047 Size: 2269 1012 2975 Fragments arranged by size: 2975 2269 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6256 BstYI r'GATC_y Cuts at: 1972 3529 3586 3953 4338 4355 5807 5893 5905 Size: 1557 57 367 385 17 1452 86 12 Cuts at: 5905 6251 1972 Size: 346 1977 Fragments arranged by size: 1977 1557 1452 385 367 346 86 57 17 12 Bsu36I CC'TnA_GG Cuts at: 2685 3534 2685 Size: 849 5407 BtgI C'CryG_G Cuts at: 513 1065 2004 2443 3489 513 Size: 552 939 439 1046 3280 Fragments arranged by size: 3280 1046 939 552 439 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5517 Fragments arranged by size: 5517 683 56 BtsI GCAGTG_nn' Cuts at: 3772 4566 4586 3772 Size: 794 20 5442 Fragments arranged by size: 5442 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2581 2616 529 Size: 35 2017 35 4169 Fragments arranged by size: 4169 2017 35 35 DraI TTT'AAA Cuts at: 2463 3560 2463 Size: 1097 5159 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3703 4592 6221 Size: 54 1425 50 275 18 1873 889 1629 Cuts at: 6221 8 Size: 43 Fragments arranged by size: 1873 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3703 1537 Size: 275 1891 4090 Fragments arranged by size: 4090 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2664 4186 1353 Size: 1311 1522 3423 Fragments arranged by size: 3423 1522 1311 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4843 5217 5363 387 Size: 1064 138 3254 374 146 1280 Fragments arranged by size: 3254 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2052 2052 Size: 6256 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2325 2786 2907 3084 3144 4319 Size: 393 533 160 461 121 177 60 1175 Cuts at: 4319 4916 5703 5971 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1175 787 597 533 461 393 268 177 160 121 60 EcoNI CCTnn'n_nnAGG Cuts at: 3537 3537 Size: 6256 EcoO109I rG'GnC_Cy Cuts at: 2173 2560 3307 2173 Size: 387 747 5122 Fragments arranged by size: 5122 747 387 EcoRI G'AATT_C Cuts at: 2969 3565 3943 2969 Size: 596 378 5282 Fragments arranged by size: 5282 596 378 EcoRV GAT'ATC Cuts at: 2034 2034 Size: 6256 FspI TGC'GCA Cuts at: 4762 4762 Size: 6256 HaeII r_GCGC'y Cuts at: 1678 2836 5403 1678 Size: 1158 2567 2531 Fragments arranged by size: 2567 2531 1158 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2705 2737 3329 3361 4897 Size: 32 135 32 1133 32 592 32 1536 Cuts at: 4897 4929 4971 5003 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1536 1133 592 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3337 3600 3846 669 Size: 988 281 1399 263 246 3079 Fragments arranged by size: 3079 1399 988 281 263 246 HindIII A'AGCT_T Cuts at: 748 3408 748 Size: 2660 3596 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5370 5554 1305 Size: 4065 184 2007 Fragments arranged by size: 4065 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4434 Fragments arranged by size: 4434 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2881 4214 4573 4732 518 Size: 648 1715 1333 359 159 2042 Fragments arranged by size: 2042 1715 1333 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2996 3358 3672 4349 5497 5742 1606 Size: 1390 362 314 677 1148 245 2120 Fragments arranged by size: 2120 1390 1148 677 362 314 245 NaeI GCC'GGC Cuts at: 2109 2109 Size: 6256 NcoI C'CATG_G Cuts at: 513 1065 2004 2443 3489 513 Size: 552 939 439 1046 3280 Fragments arranged by size: 3280 1046 939 552 439 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NgoMIV G'CCGG_C Cuts at: 2107 2107 Size: 6256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NsiI A_TGCA'T Cuts at: 3076 3076 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 2654 2992 3296 3614 5159 1132 Size: 97 1425 338 304 318 1545 2229 Fragments arranged by size: 2229 1545 1425 338 318 304 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6256 PciI A'CATG_T Cuts at: 3292 5155 3292 Size: 1863 4393 PflMI CCAn_nnn'nTGG Cuts at: 1108 2444 1108 Size: 1336 4920 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2173 2560 3307 2173 Size: 387 747 5122 Fragments arranged by size: 5122 747 387 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PstI C_TGCA'G Cuts at: 830 3608 830 Size: 2778 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 PvuII CAG'CTG Cuts at: 2996 2996 Size: 6256 SacI G_AGCT'C Cuts at: 2054 2054 Size: 6256 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SbfI CC_TGCA'GG Cuts at: 3608 3608 Size: 6256 ScaI AGT'ACT Cuts at: 2150 4504 6216 2150 Size: 2354 1712 2190 Fragments arranged by size: 2354 2190 1712 SfcI C'TryA_G Cuts at: 826 1045 2949 3262 3604 4739 5420 5611 826 Size: 219 1904 313 342 1135 681 191 1471 Fragments arranged by size: 1904 1471 1135 681 342 313 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 3583 1872 Size: 1711 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2474 4360 5261 5523 820 Size: 197 926 531 1886 901 262 1553 Fragments arranged by size: 1886 1553 926 901 531 262 197 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 2654 3614 1132 Size: 1522 960 3774 Fragments arranged by size: 3774 1522 960 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6256 StyI C'CwwG_G Cuts at: 513 1065 2004 2443 2529 3489 3695 513 Size: 552 939 439 86 960 206 3074 Fragments arranged by size: 3074 960 939 552 439 206 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2097 2680 2778 4283 4300 4453 4638 Size: 1255 517 583 98 1505 17 153 185 Cuts at: 4638 5083 325 Size: 445 1498 Fragments arranged by size: 1505 1498 1255 583 517 445 185 153 98 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 2554 4502 Size: 274 80 33 51 1443 171 406 1948 Cuts at: 4502 6214 96 Size: 1712 138 Fragments arranged by size: 1948 1712 1443 406 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6216 6248 6216 Size: 32 6224 Tth111I GACn'n_nGTC Cuts at: 1316 2375 1316 Size: 1059 5197 XbaI T'CTAG_A Cuts at: 3173 3203 3592 3173 Size: 30 389 5837 Fragments arranged by size: 5837 389 30 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3112 3112 Size: 6256 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6256 XmaI C'CCGG_G Cuts at: 1870 3581 1870 Size: 1711 4545 XmnI GAAnn'nnTTC Cuts at: 2150 4385 2150 Size: 2235 4021 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI